BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,084,426	G→T	7.2%	intergenic (‑51/+24)	PP_t55 ← / ← PP_t54	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,084,426	0	G	T	7.2%	433.0 / 14.3	145	intergenic (‑51/+24)	PP_t55/PP_t54	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base G (65/68); new base T (2/10); total (67/78)
Fisher's exact test for biased strand distribution p-value = 3.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

AAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAAGCAGCCTT  >  NC_002947/5084277‑5084575

  gCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCCCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACAAGATTACCGCTGGTTGACTGAAAAGGCTTTGCa                                                                                                                                                     >  2:5284146/1‑150 (MQ=255)

    aGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATAACCGATGGTTGAATGAAATGGCTTTGCTAg                                                                                                                                                   >  2:3238791/1‑150 (MQ=255)

     gAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGc                                                                                                                                                  <  1:2953652/150‑1 (MQ=255)

         cACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCAATCCATCTGAGCTATTAGGCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGAGTGACGCGGCGTTGCAAGCAGag                                                                                                                                              >  1:5348765/1‑149 (MQ=14)

            ttCAAGACAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCa                                                                                                                                           <  2:581111/150‑1 (MQ=255)

                tgtttttGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTc                                                                                                                                       <  2:4021746/144‑1 (MQ=255)

                agctataggCGGACCCACCACGAATCCAACCATCCACCGCCCGGTTCGTACGCCACTAACCAAACCACCCGACCCATGACGACGCTATTGGCAGTTTTACACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTc                                                                                                                                       <  2:5525690/143‑1 (MQ=255)

                    aGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAACCAGAACCACTTCAAGa                                                                                                                                   <  2:1995610/150‑1 (MQ=255)

                         cGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTg                                                                                                                              <  2:1641925/150‑1 (MQ=255)

                          ggACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTgg                                                                                                                             <  1:43661/150‑1 (MQ=255)

                          ggACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTAGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTgg                                                                                                                             <  2:13170/150‑1 (MQ=255)

                           gACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGc                                                                                                                            <  1:323442/150‑1 (MQ=255)

                            aCTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCg                                                                                                                           <  1:3679762/150‑1 (MQ=255)

                              tCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTATACCAGATAACCGATGTTTGAATGAGATGGCTTTGAAAGAATAACAAGTAAAAGAAAGAGGCGGa                                                                                                                         >  2:5199554/1‑150 (MQ=14)

                                  cattaTTCGAACCTTCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTca                                                                                                                     <  1:1470690/146‑1 (MQ=255)

                                  cagGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTca                                                                                                                     <  1:3749484/150‑1 (MQ=255)

                                     gATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTcagca                                                                                                                  <  1:2303684/150‑1 (MQ=255)

                                          gAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACAACTTCAAGAAAGTGGCGGACTCGGCAGGAtg                                                                                                             >  2:2128553/1‑149 (MQ=255)

                                           aaCCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTc                                                                                                            <  2:2715168/150‑1 (MQ=255)

                                           aaCCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTc                                                                                                            <  1:1111619/150‑1 (MQ=255)

                                           aaCCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAAAGGCTTTGCAAGCAGAACCACTTCAAGAAGGTGGCGGACTCAGCAGGATTc                                                                                                            >  2:3503907/1‑150 (MQ=255)

                                               tGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAAc                                                                                                        >  1:2384347/1‑150 (MQ=255)

                                                 cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCt                                                                                                      <  2:3399042/150‑1 (MQ=255)

                                                 cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCt                                                                                                      <  1:4742114/150‑1 (MQ=255)

                                                     cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGa                                                                                                  >  1:3908167/1‑150 (MQ=255)

                                                     cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGa                                                                                                  <  1:3921157/150‑1 (MQ=255)

                                                       cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGAGGACTCAGCAGGATTCGAACCTGCGAcc                                                                                                >  2:4576235/1‑150 (MQ=255)

                                                           gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTc                                                                                            >  1:536864/1‑150 (MQ=255)

                                                           gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTc                                                                                            >  1:195204/1‑150 (MQ=255)

                                                               gTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGtt                                                                                        <  1:2128553/150‑1 (MQ=255)

                                                                gaGCCGTGTACTCGATACAGCTGATCTATGTGTCCGCGTTTGGCAGTTTTATACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTc                                                                                       <  2:5155391/149‑1 (MQ=17)

                                                                 aGCCGAGTACTCTATCCAGCTGAGCTTTGAGTCCGCGTTTGGCAGTTTTACACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCg                                                                                      <  2:2522330/150‑1 (MQ=255)

                                                                  gCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGt                                                                                     >  1:3372637/1‑150 (MQ=255)

                                                                        ttgactATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGa                                                                               <  2:2450704/146‑1 (MQ=255)

                                                                        tACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGa                                                                               <  2:56516/150‑1 (MQ=255)

                                                                        tACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGa                                                                               <  1:1310845/150‑1 (MQ=255)

                                                                          ctctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGt                                                                             >  1:625731/1‑150 (MQ=255)

                                                                           tctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCTAGAAAGTGGCGGACTCAGCAGGATTCGAACCTg                                                                                                     <  1:3150751/125‑1 (MQ=255)

                                                                           tctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCGAGAAAGTGGCGGACTCAGCAGGATTCGAACCTg                                                                                                     >  2:3150751/1‑125 (MQ=255)

                                                                           tctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTa                                                                            >  1:1943875/1‑150 (MQ=255)

                                                                             tATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTAct                                                                          >  1:4686389/1‑150 (MQ=255)

                                                                             tATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTAct                                                                          <  2:1392314/150‑1 (MQ=255)

                                                                                ccAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                                                       <  1:5437979/150‑1 (MQ=255)

                                                                                     tGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                  >  1:4343869/1‑150 (MQ=255)

                                                                                         ccaTGAGTCCGCGATTGGCAGTTTTAACCCAGATTTCCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                              <  2:4378096/148‑1 (MQ=255)

                                                                                          tATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                             >  1:3268551/1‑150 (MQ=255)

                                                                                          tATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGAACTCTATCCAGCTGAg                                                             >  1:3847113/1‑150 (MQ=255)

                                                                                          tATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGAACTCTATACAGCTGAg                                                             >  1:3847105/1‑150 (MQ=255)

                                                                                          tATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTAGAACCTGCGACCCCACGGTTCGTAGCCCAGTACTCTATCCAGcagtg                                                             >  1:2339399/1‑146 (MQ=17)

                                                                                           aTGTGTCCGCGTTTGGCAGTTTTACACCAGTTTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                                                            <  2:3970502/150‑1 (MQ=255)

                                                                                              aggccGCGTTTGGCAGTTTTATACCAGATTACCTCTGGTTGACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt                                                         <  2:1078220/147‑1 (MQ=14)

                                                                                                  cgcgATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGt                                                     >  1:3212021/1‑150 (MQ=255)

                                                                                                   gcgATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTc                                                    >  1:3988255/1‑150 (MQ=255)

                                                                                                   gcgATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTc                                                    >  1:2503860/1‑150 (MQ=255)

                                                                                                       ttGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCa                                                >  1:1253583/1‑150 (MQ=255)

                                                                                                          gCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAAGCTCTTCAAGTAAGTGGCGGACTCTGCAGGATTCGATCCTGCGAGCGGTGGGTTCGTTGCCGAGTACTCTATGCTGGTGTGCTATGAGTCCGGAAtt                                             >  2:4139973/1‑150 (MQ=255)

                                                                                                          gCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCgatagt                                             >  1:5623988/1‑145 (MQ=255)

                                                                                                          gCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGcgagt                                             >  2:4072032/1‑146 (MQ=255)

                                                                                                          gCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAtt                                             >  1:3036968/1‑150 (MQ=255)

                                                                                                          gCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAtt                                             >  2:5099799/1‑150 (MQ=255)

                                                                                                          gCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAtt                                             <  2:2384347/150‑1 (MQ=255)

                                                                                                           cAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCaaaag                                            >  1:5137359/1‑147 (MQ=255)

                                                                                                            aGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTgg                                           >  1:5001395/1‑150 (MQ=255)

                                                                                                            aGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTgg                                           >  1:1062677/1‑150 (MQ=255)

                                                                                                                ttAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGt                                       >  1:5094655/1‑150 (MQ=255)

                                                                                                                ttAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGATCAGCTATGAGACCGAAATTggaaca                                       >  1:3433441/1‑146 (MQ=14)

                                                                                                                   caCCAGATGACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCTCTTCAAGAACGTGGCGGCCTCAGCAGGCTTCGACCCTGCGACCGCTCGGTTCGTTGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCTGtttt                                    <  2:3860180/149‑1 (MQ=14)

                                                                                                                   aaCCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGCCAGtttt                                    >  2:4966617/1‑150 (MQ=255)

                                                                                                                    aCCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCaa                                               <  2:691336/138‑1 (MQ=255)

                                                                                                                    aCCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCCAATGGCAgataaa                                   >  2:1835215/1‑145 (MQ=255)

                                                                                                                    aCCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTa                                   <  2:4686389/150‑1 (MQ=255)

                                                                                                                    aCCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAGTGGCAggtgta                                   >  2:1835175/1‑145 (MQ=255)

                                                                                                                      cAGCTTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGa                                 <  2:2084084/150‑1 (MQ=255)

                                                                                                                       aGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGAc                                <  2:1788764/150‑1 (MQ=255)

                                                                                                                         aTTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAtt                                             >  1:1850875/1‑135 (MQ=255)

                                                                                                                         aTTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAtt                                             <  2:1851122/135‑1 (MQ=255)

                                                                                                                              cGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCg                                                                                      <  1:3550866/89‑1 (MQ=255)

                                                                                                                                  ggcttaCTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCt                     <  2:3856583/145‑1 (MQ=37)

                                                                                                                                  ggattaCTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCt                     <  1:976903/145‑1 (MQ=19)

                                                                                                                                    ctGACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTgg                   <  1:4251537/149‑1 (MQ=38)

                                                                                                                                     tGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACGGATGGt                  >  2:1655178/1‑150 (MQ=255)

                                                                                                                                         tGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt                                                         <  2:2823104/107‑1 (MQ=32)

                                                                                                                                         tGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt                                                         >  1:2823104/1‑107 (MQ=32)

                                                                                                                                         tGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgat              <  2:195204/150‑1 (MQ=255)

                                                                                                                                         tGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCGATGAGTCCGCAATTGGCAGTGTTAGACCAGATACCGCTGGttgat              >  1:4931234/1‑150 (MQ=255)

                                                                                                                                          gAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgatt             <  1:2529633/150‑1 (MQ=38)

                                                                                                                                          gAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgatt             <  1:3238791/150‑1 (MQ=255)

                                                                                                                                          gAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgatt             <  1:1795139/150‑1 (MQ=255)

                                                                                                                                           aaaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGa                                 <  2:2138848/129‑1 (MQ=14)

                                                                                                                                            aaTGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgattga           >  2:4678333/1‑150 (MQ=255)

                                                                                                                                             aTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGaa          <  2:5314111/150‑1 (MQ=38)

                                                                                                                                             aTGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                                     <  1:633612/91‑1 (MQ=33)

                                                                                                                                              tGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGtt                 <  2:1706311/142‑1 (MQ=25)

                                                                                                                                              tGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGtt                 >  1:1706311/1‑142 (MQ=25)

                                                                                                                                               ggCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCCATTGGCAGTTTTAGACCAGATACCGCGGGTTGAATGGaga        >  2:2569008/1‑149 (MQ=11)

                                                                                                                                                gCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAagca       >  1:5178010/1‑150 (MQ=255)

                                                                                                                                                 cTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCTATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAagcag      <  1:2845091/150‑1 (MQ=255)

                                                                                                                                                 cTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAagcag      <  1:1244546/150‑1 (MQ=255)

                                                                                                                                                 cTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAagcag      <  1:5069039/150‑1 (MQ=255)

                                                                                                                                                     gCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAAGCAGCCtt  <  1:1686759/150‑1 (MQ=255)

AAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAAGCAGCCTT  >  NC_002947/5084277‑5084575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`