BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,102,237	A→C	6.4%	intergenic (+231/+61)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,102,237	0	A	C	6.4%	299.8 / 5.2	109	intergenic (+231/+61)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (63/36); new base C (3/6); total (67/42)
Fisher's exact test for biased strand distribution p-value = 8.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

TGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGG  >  NC_002947/5102088‑5102386

tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                                       <  1:5154352/150‑1 (MQ=255)

tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                                       <  1:5494108/150‑1 (MQ=255)

tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                                       <  1:4215536/150‑1 (MQ=255)

tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                                       <  1:2804184/150‑1 (MQ=255)

tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCT‑GCGCCCCGAACCTGTGCGGGACCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAg                                                                                                                                                      <  1:4832683/150‑1 (MQ=255)

 gCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAg                                                                                                                                                      <  2:2629457/150‑1 (MQ=255)

   tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGGTTTCCCCCGCATTCGGGTGGGCCGGGGGCAGCG‑TAGcc                                                                                                                                                    >  1:935381/1‑150 (MQ=255)

     aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                                                  >  2:4486613/1‑150 (MQ=255)

       tCCGATGCACCGAATCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                <  1:3254362/150‑1 (MQ=255)

       tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                <  1:3254476/150‑1 (MQ=255)

       tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                <  2:1479583/150‑1 (MQ=255)

       tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                >  2:5610061/1‑150 (MQ=255)

       tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCGTTCCCGCGAATGCGTGAGGTCAGGGGGCCGCT‑TAGCCTGaa                                                                                                                                                >  1:3770987/1‑150 (MQ=255)

       tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCGTGCCCCCGAAAGCGGGAGGTCAGGGGACAGCT‑TTGCCTggc                                                                                                                                                >  1:3429531/1‑148 (MQ=255)

           aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGa                                                                                                                                            <  2:4579068/150‑1 (MQ=255)

           aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCGGAACTGa                                                                                                                                            >  1:2330917/1‑150 (MQ=255)

           aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCGTTCCCGCGAAATCGGGAGGGCAGGGGGCCGCG‑TAGCCTGAACTGa                                                                                                                                            >  1:4865364/1‑150 (MQ=255)

            tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                           <  1:1004385/150‑1 (MQ=255)

            tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                           <  2:2424552/150‑1 (MQ=255)

                    aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                                                                                                                                   <  1:734083/150‑1 (MQ=255)

                    aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                                                                                                                                   <  1:552710/150‑1 (MQ=255)

                     aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAGGAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                                  <  2:628858/150‑1 (MQ=18)

                     aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                  <  2:4278259/150‑1 (MQ=255)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                 <  1:2280064/150‑1 (MQ=255)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                 <  2:3978454/150‑1 (MQ=255)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                 <  2:4496184/150‑1 (MQ=255)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                 <  1:1746098/150‑1 (MQ=255)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                 <  2:1676035/150‑1 (MQ=255)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                 <  2:1676715/150‑1 (MQ=255)

                       gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGGATGCGGGAGGTCAGGGGACAGCT‑TAGCCGGAACGGACGCAGTCGCcgg                                                                                                                                >  1:4896608/1‑150 (MQ=255)

                       gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                                                                <  1:644707/150‑1 (MQ=255)

                          ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCaa                                                                                                                             <  1:2664845/150‑1 (MQ=255)

                            ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGCATGCGGGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                           >  2:3001066/1‑150 (MQ=255)

                            ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                           >  2:3853404/1‑150 (MQ=255)

                                  gTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTc                                                                                                                     >  1:5094062/1‑150 (MQ=255)

                                        tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCCGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAg                                                                                                               >  2:3682296/1‑150 (MQ=255)

                                         ccggcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGGTTGCCCGCGAAATCGTTATGTGGTGGGTCAGCT‑TATGCTTAAAATACGCAAAAGCGGGCAAACCCGCTCCTGCaag                                                                                                              >  1:1964990/4‑148 (MQ=255)

                                          gcgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGGCCGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCGGCAggg                                                                                                             >  2:3167210/1‑150 (MQ=255)

                                          gcgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGGCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACCGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCACCTGCAggg                                                                                                             >  2:3167204/1‑150 (MQ=255)

                                               ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCGTGCCCCCGAAAGCGTGTGGTCCGGGGACAGCT‑TAGCCTGGACAGACGCAATAGCCGGCAAACCCCCTCCAGCAGGGGGGAt                                                                                                        >  1:3909874/1‑150 (MQ=255)

                                                 gAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGTCCGGGGGCAGCG‑TAGCCGGAACTGACGCATTCGCGGGCAAGCCCGCACCAGCAGGGGGTATTg                                                                                                      >  2:490835/1‑150 (MQ=255)

                                                      tgtgCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGGCAGGGGACAGCT‑TAGCCGGAACGGACGCATTCGCGGGCCAGCCCGCTCCTGCAGGGGGGATGGCCggg                                                                                                 >  2:5367857/1‑150 (MQ=255)

                                                        ggCGGGGCCGGTGACAGAACCGCACCCGCGGTTTGT‑GGGTAACCCCGCCAACCGGGGGGTGGCCCGCGACGGGGGGCGGTCCGGGGACCGCT‑TCGCCTCACCTGCCGCTTTCGGGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                               <  2:4982169/149‑1 (MQ=255)

                                                          cGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAgg                                                                                             >  2:1263218/1‑150 (MQ=255)

                                                           ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAATGCGGGCTTGCCCGCGAATGCGTGTGGGCAGGGGACCGCT‑TAGCCGGAACTGACGCATTCGCGGGCCAGCCCGCGCCGGCCGGGGGTGTTGCGGGGGAggg                                                                                            >  2:4729954/1‑150 (MQ=255)

                                                           ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGGTTGCCCGCGAAAGCGGGAGGTGAGGGGACAGCG‑TATCCTGGAATGACGCATTAGCGGGCAAGCCCGCTCCGGCAGGGGGTATTTGCGGGAAggg                                                                                            >  1:730128/1‑150 (MQ=255)

                                                                      ccgAACATTAACCTATGTTTGTCGGGCAACAAGC‑CAAACGCGGGCTGGCCCGCGAAGGGGTGAGTTCAGGGGACCGCT‑TAGCCTGAACTGACGCATTCGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGaa                                                                                 <  2:2729917/148‑1 (MQ=255)

                                                                       aGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGGATTGCCGGGAAGGGTTTACGCCGAAg                                                                                >  2:3970917/1‑150 (MQ=255)

                                                                       aGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCGTGCCCGCGAATGCGTGAGGTCAGGGGCCACGG‑TAGCCTGACCGGACGCATTCGCGGGCCAACCCGCGCCTGCAGGGGGTTTTTCCGGGGCGGGTTTACGCCGAAg                                                                                >  1:2937210/1‑150 (MQ=255)

                                                                          aCAGTAACCGATGATTGTCGGGTAACAAGG‑CAAACGCGGGCTTGCCCGCGATGGCGTGTGGTCAGGGGACAGCT‑TAGCCTGACCTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCa                                                                             <  2:4637955/150‑1 (MQ=255)

                                                                            aGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCGTGCCCCCGAATGCGGGAGGTCCGGGGACAGCT‑TAGCCGGAACTGACGCAATAGCGGGCAAGCCCCCTCCGGCAGGGGGTATTGCCGGGAAGGGTTTAAGCCCAAGCCAgg                                                                           >  1:864290/1‑150 (MQ=255)

                                                                              tAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCCCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCGGAACAGACGCAAACGCCGGCAAGCCCCCGCCAGCAGGGGGTATTGCCGGGAAGGGGTTACGCCGAAGCCAGGtt                                                                         >  1:4671513/1‑150 (MQ=255)

                                                                               aaCCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACAGACGCATTCGCGGGCAAGCCCGCTCCAGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTc                                                                        >  2:2842918/1‑150 (MQ=255)

                                                                                cccGATGATGGTCGGTTAACAATG‑CAAACGGGGGGTTGCCCGCGAAGGCGTGAGGTCCGGGGACATCT‑TAGCCTGAACTGCCGCTTTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCa                                                                       <  2:2220205/149‑1 (MQ=255)

                                                                                cccGAGGATTGGCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGGGCGGTCCGGGGCCATCT‑TAGCCTGAACTGACGCATACGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCa                                                                       <  2:2220179/149‑1 (MQ=255)

                                                                                 ccGATGATTGTCGGGTAACACTG‑CAAAAGCGGGCTTGCCGGCGAATGCGTGAGGTCCGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCAt                                                                      <  2:5065089/150‑1 (MQ=255)

                                                                                 ccGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                  <  2:326303/90‑1 (MQ=255)

                                                                                 ccGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                  >  1:326303/1‑90 (MQ=255)

                                                                                  cGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTTCCCCCGAATTCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAATGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCCGGAAGGGTTTAAGCCGAAGCCCGGTTAATc                                                                     >  2:1430519/1‑150 (MQ=255)

                                                                                  cGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGCAAGCGTGAGGGCAGGGGACAGCT‑TAGCCAGAACTGACCCAATCGCGGGCAAGCCCGCACCAGCAGGGGGTATTGCCGGGCAGGGTTTACGCCGAAGCCAGGTTCaaa                                                                     >  2:1430473/1‑148 (MQ=255)

                                                                                  cGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCCCGAAAGCGTGATGTCAGGGGCCAGCG‑TAGCCAGAAAGGACCCATACGCGGGCAAGCCCCCACCAGCACGGGGGATTGCCGGGAAGGGGTTACGCCCAACCCCGGTTCATc                                                                     >  2:3017685/1‑150 (MQ=255)

                                                                                             gggTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACTGGCGCATTCGCGGGCAAGCCCGCTCCTGCCGGGGGTCTTGGCGGGAAGGGTTTACGCCGAAGCCCGGTTCATCGCCTTGTGGGt                                                          >  2:1750876/1‑150 (MQ=255)

                                                                                              ggTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACCGCT‑TAGCCTGAACGGACGCATTCGCGGGCAAGCCCGCGCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTa                                                         >  2:4487554/1‑150 (MQ=255)

                                                                                              ggTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TATCCGGAACAGACGCATTCGCGGGCAAGCCCGCTCCGGGAGGGGGTATTGCCGGGAAGGGGTTACGCCGAAGCCAGGTTCATCGCCGTGTGGGTa                                                         >  2:1640248/1‑150 (MQ=255)

                                                                                                     attccAAACGCGGGCTGGCCGGCGAATGGGTGCGGCCGGGGGACAGAT‑TCGCCTGAAGCCACGCATGCGGGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATc                                                   <  2:3859662/146‑1 (MQ=255)

                                                                                                       g‑CAAACGCGGGGGTGCCCGCGAATGCGTGAGGTCAGGGCACAGCT‑TAGCCTGAACGGACGCATGCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAgg                                                <  2:3156462/150‑1 (MQ=255)

                                                                                                         caaccgCGGGCTTGCCCGCTACTGCGTGCGGTCTGGGGACCGCT‑TAGCCTCACCTGCCGCTTCCGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGt                                               <  2:579887/145‑1 (MQ=255)

                                                                                                         cAAACGGGGGCTGGCCCGCTAATGGTTGTGGTCAGGGGACCGCT‑TAGCCTTACCTGACGCATTCCCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGt                                               <  2:3457350/150‑1 (MQ=255)

                                                                                                            acgggggCTTGCCCGCGAATGCGTGTGGTCTGGGGACAGCT‑TAGCCCGACCTCCCGCTTTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCt                                            <  2:1759125/146‑1 (MQ=255)

                                                                                                                  gCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                                      >  1:3094746/1‑150 (MQ=18)

                                                                                                                       cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                                 >  2:3404244/1‑150 (MQ=255)

                                                                                                                         cgcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                               >  2:2464582/1‑150 (MQ=255)

                                                                                                                         cgcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGACGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                               >  1:4080327/1‑150 (MQ=21)

                                                                                                                         cgcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                               >  1:1414898/1‑150 (MQ=21)

                                                                                                                          gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCTTTCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                              <  2:2240806/150‑1 (MQ=255)

                                                                                                                          gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                              >  1:2778227/1‑150 (MQ=255)

                                                                                                                           cgatggggtgtGGTCGGGGCACCGCT‑TAGCCTGAAGTGCCCCTTGCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGt                             <  2:603929/143‑1 (MQ=255)

                                                                                                                              aTGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAg                          >  1:2571579/1‑150 (MQ=255)

                                                                                                                                  gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTCTTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                      >  2:1191128/1‑150 (MQ=255)

                                                                                                                                  gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                      >  2:5355450/1‑150 (MQ=255)

                                                                                                                                  gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                      >  2:529906/1‑150 (MQ=255)

                                                                                                                                  gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                      >  2:590087/1‑150 (MQ=255)

                                                                                                                                       gTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg                 >  1:3653682/1‑150 (MQ=255)

                                                                                                                                        tctggggACTGCT‑TCGCCTCAATGGCCGCTTTGGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                <  1:5082286/147‑1 (MQ=255)

                                                                                                                                        tctggggACCGCT‑TCGCCTCACCTGCCGCATGGGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                <  2:2848845/147‑1 (MQ=255)

                                                                                                                                        tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                >  1:1226334/1‑150 (MQ=255)

                                                                                                                                          ggggtACAGTT‑TCGCCTTAACTGCGGCATTCGGGGCAAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga              <  2:3770987/149‑1 (MQ=255)

                                                                                                                                          aGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga              >  1:307061/1‑150 (MQ=255)

                                                                                                                                            gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata            >  2:5020782/1‑150 (MQ=255)

                                                                                                                                            gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGAAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata            >  1:802641/1‑150 (MQ=255)

                                                                                                                                             ggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGatat           >  2:2295901/1‑150 (MQ=255)

                                                                                                                                                 aGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc       >  1:206346/1‑150 (MQ=255)

                                                                                                                                                   cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa     >  2:523784/1‑150 (MQ=255)

                                                                                                                                                   cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa     >  2:523793/1‑150 (MQ=255)

                                                                                                                                                   cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa     >  2:523985/1‑150 (MQ=255)

                                                                                                                                                   cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa     >  2:1433527/1‑150 (MQ=255)

                                                                                                                                                    t‑tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg    >  1:4781107/1‑150 (MQ=255)

                                                                                                                                                      tAGCGTGAAGTGACTATTTCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGATGGGTTTACGCCGAAGCCTGGTTCATCGCCTTGTGGGTATCTATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAgg   <  2:2923296/150‑1 (MQ=255)

                                                                                                                                                      tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAgg   >  2:4697367/1‑150 (MQ=255)

                                                                                                                                                       aGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAggg  >  1:4812461/1‑150 (MQ=255)

TGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGG  >  NC_002947/5102088‑5102386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`