BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,102,244	A→C	9.1%	intergenic (+238/+54)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,102,244	0	A	C	9.1%	305.6 / 8.0	107	intergenic (+238/+54)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (65/30); new base C (4/8); total (69/38)
Fisher's exact test for biased strand distribution p-value = 2.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCG  >  NC_002947/5102095‑5102391

tCCGATGCACCGAATCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                     <  1:3254362/150‑1 (MQ=255)

tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGGTTGCCCCCGAATGGGTGGGGTCAGGGGACAGCG‑TAGCCTGaa                                                                                                                                                     >  1:4887048/1‑150 (MQ=255)

tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                     <  2:1479583/150‑1 (MQ=255)

tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                     >  2:5610061/1‑150 (MQ=255)

tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                     <  1:3254476/150‑1 (MQ=255)

tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCGTTCCCGCGAATGCGTGAGGTCAGGGGGCCGCT‑TAGCCTGaa                                                                                                                                                     >  1:3770987/1‑150 (MQ=255)

    aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGa                                                                                                                                                 <  2:4579068/150‑1 (MQ=255)

    aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCGGAACTGa                                                                                                                                                 >  1:2330917/1‑150 (MQ=255)

    aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCGTTCCCGCGAAATCGGGAGGGCAGGGGGCCGCG‑TAGCCTGAACTGa                                                                                                                                                 >  1:4865364/1‑150 (MQ=255)

     tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                                <  1:1004385/150‑1 (MQ=255)

     tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                                <  2:2424552/150‑1 (MQ=255)

             aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                                                                                                                                        <  1:734083/150‑1 (MQ=255)

             aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                                                                                                                                        <  1:552710/150‑1 (MQ=255)

              aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAGGAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                                       <  2:628858/150‑1 (MQ=18)

              aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                       <  2:4278259/150‑1 (MQ=255)

               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                      <  2:4496184/150‑1 (MQ=255)

               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                      <  1:1746098/150‑1 (MQ=255)

               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                      <  2:1676035/150‑1 (MQ=255)

               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                      <  2:3978454/150‑1 (MQ=255)

                gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGGATGCGGGAGGTCAGGGGACAGCT‑TAGCCGGAACGGACGCAGTCGCcgg                                                                                                                                     >  1:4896608/1‑150 (MQ=255)

                gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                                                                     <  1:644707/150‑1 (MQ=255)

                   ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCaa                                                                                                                                  <  1:2664845/150‑1 (MQ=255)

                     ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                                >  2:3853404/1‑150 (MQ=255)

                                 tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCCGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAg                                                                                                                    >  2:3682296/1‑150 (MQ=255)

                                 tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCA‑TAGCCAGAAAAGACGCAATCGCGGGCAAGCCCGCTCCTGCAg                                                                                                                    >  2:1690456/1‑150 (MQ=255)

                                  ccggcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGGTTGCCCGCGAAATCGTTATGTGGTGGGTCAGCT‑TATGCTTAAAATACGCAAAAGCGGGCAAACCCGCTCCTGCaag                                                                                                                   >  1:1964990/4‑148 (MQ=255)

                                   gcgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGGCCGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCGGCAggg                                                                                                                  >  2:3167210/1‑150 (MQ=255)

                                   gcgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGGCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACCGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCACCTGCAggg                                                                                                                  >  2:3167204/1‑150 (MQ=255)

                                        ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCGTGCCCCCGAAAGCGTGTGGTCCGGGGACAGCT‑TAGCCTGGACAGACGCAATAGCCGGCAAACCCCCTCCAGCAGGGGGGAt                                                                                                             >  1:3909874/1‑150 (MQ=255)

                                               tgtgCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGGCAGGGGACAGCT‑TAGCCGGAACGGACGCATTCGCGGGCCAGCCCGCTCCTGCAGGGGGGATGGCCggg                                                                                                      >  2:5367857/1‑150 (MQ=255)

                                                 tgCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                                    >  2:3747845/1‑150 (MQ=255)

                                                 tgCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCAGCAGGGGGTATTGCCGGGaa                                                                                                    >  2:2485257/1‑150 (MQ=255)

                                                 ggCGGGGCCGGTGACAGAACCGCACCCGCGGTTTGT‑GGGTAACCCCGCCAACCGGGGGGTGGCCCGCGACGGGGGGCGGTCCGGGGACCGCT‑TCGCCTCACCTGCCGCTTTCGGGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                                    <  2:4982169/149‑1 (MQ=255)

                                                   cGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAgg                                                                                                  >  2:1263218/1‑150 (MQ=255)

                                                    ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAATGCGGGCTTGCCCGCGAATGCGTGTGGGCAGGGGACCGCT‑TAGCCGGAACTGACGCATTCGCGGGCCAGCCCGCGCCGGCCGGGGGTGTTGCGGGGGAggg                                                                                                 >  2:4729954/1‑150 (MQ=255)

                                                    ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGGTTGCCCGCGAAAGCGGGAGGTGAGGGGACAGCG‑TATCCTGGAATGACGCATTAGCGGGCAAGCCCGCTCCGGCAGGGGGTATTTGCGGGAAggg                                                                                                 >  1:730128/1‑150 (MQ=255)

                                                          ggTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGGGGTCAGGGGGCAGCT‑TAGCCGGTACTGACGCAATAGCGGGCCAGCCCGCTCCGGCAGGGGGTGTTGCCGGGAAGGGGTTaag                                                                                           >  1:4024954/1‑148 (MQ=255)

                                                              aCAGAACATTCCCCGATTTTTGTCGGTTAACAATG‑AAAACGGGGGGTTGCCCGCGATTGCGTGTGGACTGGGGACAGCT‑TAGCCTGACGTCCCGCTTTCGCGGCCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGa                                                                                       <  2:4919072/150‑1 (MQ=255)

                                                               ccgAACATTAACCTATGTTTGTCGGGCAACAAGC‑CAAACGCGGGCTGGCCCGCGAAGGGGTGAGTTCAGGGGACCGCT‑TAGCCTGAACTGACGCATTCGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGaa                                                                                      <  2:2729917/148‑1 (MQ=255)

                                                                aGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGGATTGCCGGGAAGGGTTTACGCCGAAg                                                                                     >  2:3970917/1‑150 (MQ=255)

                                                                aGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCGTGCCCGCGAATGCGTGAGGTCAGGGGCCACGG‑TAGCCTGACCGGACGCATTCGCGGGCCAACCCGCGCCTGCAGGGGGTTTTTCCGGGGCGGGTTTACGCCGAAg                                                                                     >  1:2937210/1‑150 (MQ=255)

                                                                   aCAGTAACCGATGATTGTCGGGTAACAAGG‑CAAACGCGGGCTTGCCCGCGATGGCGTGTGGTCAGGGGACAGCT‑TAGCCTGACCTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCa                                                                                  <  2:4637955/150‑1 (MQ=255)

                                                                     aGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCGTGCCCCCGAATGCGGGAGGTCCGGGGACAGCT‑TAGCCGGAACTGACGCAATAGCGGGCAAGCCCCCTCCGGCAGGGGGTATTGCCGGGAAGGGTTTAAGCCCAAGCCAgg                                                                                >  1:864290/1‑150 (MQ=255)

                                                                       tAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCCCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCGGAACAGACGCAAACGCCGGCAAGCCCCCGCCAGCAGGGGGTATTGCCGGGAAGGGGTTACGCCGAAGCCAGGtt                                                                              >  1:4671513/1‑150 (MQ=255)

                                                                        aaCCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACAGACGCATTCGCGGGCAAGCCCGCTCCAGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTc                                                                             >  2:2842918/1‑150 (MQ=255)

                                                                         cccGATGATGGTCGGTTAACAATG‑CAAACGGGGGGTTGCCCGCGAAGGCGTGAGGTCCGGGGACATCT‑TAGCCTGAACTGCCGCTTTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCa                                                                            <  2:2220205/149‑1 (MQ=255)

                                                                         cccGAGGATTGGCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGGGCGGTCCGGGGCCATCT‑TAGCCTGAACTGACGCATACGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCa                                                                            <  2:2220179/149‑1 (MQ=255)

                                                                          ccGATGATTGTCGGGTAACACTG‑CAAAAGCGGGCTTGCCGGCGAATGCGTGAGGTCCGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCAt                                                                           <  2:5065089/150‑1 (MQ=255)

                                                                          ccGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                       <  2:326303/90‑1 (MQ=255)

                                                                          ccGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                       >  1:326303/1‑90 (MQ=255)

                                                                           cGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTTCCCCCGAATTCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAATGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCCGGAAGGGTTTAAGCCGAAGCCCGGTTAATc                                                                          >  2:1430519/1‑150 (MQ=255)

                                                                           cGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGCAAGCGTGAGGGCAGGGGACAGCT‑TAGCCAGAACTGACCCAATCGCGGGCAAGCCCGCACCAGCAGGGGGTATTGCCGGGCAGGGTTTACGCCGAAGCCAGGTTCaaa                                                                          >  2:1430473/1‑148 (MQ=255)

                                                                           cGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCCCGAAAGCGTGATGTCAGGGGCCAGCG‑TAGCCAGAAAGGACCCATACGCGGGCAAGCCCCCACCAGCACGGGGGATTGCCGGGAAGGGGTTACGCCCAACCCCGGTTCATc                                                                          >  2:3017685/1‑150 (MQ=255)

                                                                                    tcggtaaaCATTG‑CAAACGCGGGGTTGCCCGCGAATGGGTGAGGTCAGGGGACAGCT‑TAGCCCCAACTGCCGCATTCGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                                 <  2:3131418/144‑1 (MQ=255)

                                                                                      gggTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACTGGCGCATTCGCGGGCAAGCCCGCTCCTGCCGGGGGTCTTGGCGGGAAGGGTTTACGCCGAAGCCCGGTTCATCGCCTTGTGGGt                                                               >  2:1750876/1‑150 (MQ=255)

                                                                                      gggTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCATGTACTGACGCATTCGCGGGCAAGCCCGCTCCTGCCGGGGGTATTTCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGt                                                               >  2:1267429/1‑150 (MQ=255)

                                                                                       ggTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACCGCT‑TAGCCTGAACGGACGCATTCGCGGGCAAGCCCGCGCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTa                                                              >  2:4487554/1‑150 (MQ=255)

                                                                                       ggTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TATCCGGAACAGACGCATTCGCGGGCAAGCCCGCTCCGGGAGGGGGTATTGCCGGGAAGGGGTTACGCCGAAGCCAGGTTCATCGCCGTGTGGGTa                                                              >  2:1640248/1‑150 (MQ=255)

                                                                                       ggTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACACCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCAGCAGGGGGTATTGCCGGGCAGGGGTTACGCCGAAGCCAGGTTCATAGCCTTGTGGGTa                                                              >  2:4487327/1‑150 (MQ=39)

                                                                                              attccAAACGCGGGCTGGCCGGCGAATGGGTGCGGCCGGGGGACAGAT‑TCGCCTGAAGCCACGCATGCGGGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATc                                                        <  2:3859662/146‑1 (MQ=255)

                                                                                                g‑CAAACGCGGGGGTGCCCGCGAATGCGTGAGGTCAGGGCACAGCT‑TAGCCTGAACGGACGCATGCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAgg                                                     <  2:3156462/150‑1 (MQ=255)

                                                                                                  caaccgCGGGCTTGCCCGCTACTGCGTGCGGTCTGGGGACCGCT‑TAGCCTCACCTGCCGCTTCCGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGt                                                    <  2:579887/145‑1 (MQ=255)

                                                                                                     acgggggCTTGCCCGCGAATGCGTGTGGTCTGGGGACAGCT‑TAGCCCGACCTCCCGCTTTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCt                                                 <  2:1759125/146‑1 (MQ=255)

                                                                                                           gCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                                           >  1:3094746/1‑150 (MQ=18)

                                                                                                                cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                                      >  2:3404244/1‑150 (MQ=255)

                                                                                                                 ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                                     >  1:2401842/1‑150 (MQ=255)

                                                                                                                 ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                                     >  1:652510/1‑150 (MQ=255)

                                                                                                                  cgcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                                    >  2:2464582/1‑150 (MQ=255)

                                                                                                                  cgcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                                    >  1:1414898/1‑150 (MQ=21)

                                                                                                                   gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                                   >  1:2778227/1‑150 (MQ=255)

                                                                                                                    cgatggggtgtGGTCGGGGCACCGCT‑TAGCCTGAAGTGCCCCTTGCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGt                                  <  2:603929/143‑1 (MQ=255)

                                                                                                                       aTGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAg                               >  1:2571579/1‑150 (MQ=255)

                                                                                                                           gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                           >  2:5355450/1‑150 (MQ=255)

                                                                                                                           gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                           >  2:590087/1‑150 (MQ=255)

                                                                                                                           gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                           >  2:529906/1‑150 (MQ=255)

                                                                                                                                gTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg                      >  1:3653682/1‑150 (MQ=255)

                                                                                                                                 tctggggACTGCT‑TCGCCTCAATGGCCGCTTTGGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                     <  1:5082286/147‑1 (MQ=255)

                                                                                                                                 tctggggACCGCT‑TCGCCTCACCTGCCGCATGGGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                     <  2:2848845/147‑1 (MQ=255)

                                                                                                                                 tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                     >  1:1226334/1‑150 (MQ=255)

                                                                                                                                   ggggtACAGTT‑TCGCCTTAACTGCGGCATTCGGGGCAAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga                   <  2:3770987/149‑1 (MQ=255)

                                                                                                                                   aGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga                   >  1:307061/1‑150 (MQ=255)

                                                                                                                                     gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata                 >  2:5020782/1‑150 (MQ=255)

                                                                                                                                     gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGAAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata                 >  1:802641/1‑150 (MQ=255)

                                                                                                                                      ggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGatat                >  2:2295901/1‑150 (MQ=255)

                                                                                                                                          aGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc            >  1:206346/1‑150 (MQ=255)

                                                                                                                                            cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa          >  2:523784/1‑150 (MQ=255)

                                                                                                                                            cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa          >  2:523985/1‑150 (MQ=255)

                                                                                                                                            cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa          >  2:523793/1‑150 (MQ=255)

                                                                                                                                            cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa          >  2:1433527/1‑150 (MQ=255)

                                                                                                                                             t‑tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg         >  1:4781107/1‑150 (MQ=255)

                                                                                                                                               tAGCGTGAAGTGACTATTTCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGATGGGTTTACGCCGAAGCCTGGTTCATCGCCTTGTGGGTATCTATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAgg        <  2:2923296/150‑1 (MQ=255)

                                                                                                                                               tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAgg        >  2:4697367/1‑150 (MQ=255)

                                                                                                                                                aGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAggg       >  1:4812461/1‑150 (MQ=255)

                                                                                                                                                 gCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCAAGGGc      >  2:2051399/1‑150 (MQ=255)

                                                                                                                                                    tGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTg                                                >  1:3449898/1‑105 (MQ=255)

                                                                                                                                                    tGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTg                                                <  2:3449898/105‑1 (MQ=255)

                                                                                                                                                    tGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtc   >  2:1082247/1‑150 (MQ=255)

                                                                                                                                                    tGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtc   >  2:1875435/1‑150 (MQ=255)

                                                                                                                                                    tGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtc   >  1:2088803/1‑150 (MQ=255)

                                                                                                                                                    tGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtc   >  1:2088277/1‑150 (MQ=255)

TCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATG‑CAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCG  >  NC_002947/5102095‑5102391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`