BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,755,403	C→T	6.9%	V389M (GTG→ATG)	thiI ←	tRNA sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,755,403	0	C	T	6.9%	852.1 / 34.5	275	V389M (GTG→ATG)	thiI	tRNA sulfurtransferase
Reads supporting (aligned to +/- strand): ref base C (134/121); new base T (12/7); total (147/128)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

GTAGAACGGCATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGG  >  NC_002947/5755254‑5755552

gTAGAACGGCATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATcac                                                                                                                                                       >  2:1285797/1‑150 (MQ=255)

    aaCGGCATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGa                                                                                                                                                   <  2:908843/150‑1 (MQ=255)

         cATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGAt                                                                                                                                              <  2:4317863/150‑1 (MQ=255)

             gCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGaa                                                                                                                                          >  2:2500619/1‑150 (MQ=255)

             gCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGaa                                                                                                                                          >  1:727614/1‑150 (MQ=255)

              ccTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGaaa                                                                                                                                         <  2:4004918/150‑1 (MQ=255)

               cTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAAt                                                                                                                                        >  1:1605611/1‑150 (MQ=255)

               cTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGAGGGAAAt                                                                                                                                        >  2:393004/1‑150 (MQ=255)

                tGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCATGTGATCGATgagctgg                                                                                                                                       <  1:440370/150‑7 (MQ=255)

                 ggACGTCGATACCGTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCCCATCGATCATGTGATCGATgagctggg                                                                                                                                      <  2:2840176/150‑8 (MQ=255)

                      ccGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTg                                                                                                                                 <  1:1073204/149‑1 (MQ=255)

                        gATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGgc                                                                                                                               <  2:267141/150‑1 (MQ=255)

                        gATACCGTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCCCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGgc                                                                                                                               <  2:4534797/150‑1 (MQ=255)

                          tACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGgcgc                                                                                                                             <  1:978703/150‑1 (MQ=255)

                             cTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCt                                                                                                                          <  2:185193/150‑1 (MQ=255)

                                ccAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCaa                                                                                                                       <  2:4223756/150‑1 (MQ=255)

                                  aGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAaga                                                                                                                     <  2:2919299/150‑1 (MQ=255)

                                  aGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAaga                                                                                                                     <  2:1571270/150‑1 (MQ=255)

                                  aGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAaga                                                                                                                     <  1:4834383/150‑1 (MQ=255)

                                     aCCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcg                                                                                                                  >  2:5514600/1‑150 (MQ=255)

                                     aCCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcg                                                                                                                  >  1:855621/1‑150 (MQ=255)

                                      ccAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcgc                                                                                                                 <  2:3960813/150‑1 (MQ=255)

                                      ccAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcgc                                                                                                                 >  1:3848797/1‑150 (MQ=255)

                                       cAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcgcg                                                                                                                >  1:210353/1‑150 (MQ=255)

                                        agagGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcgcgc                                                                                                               >  2:234509/1‑150 (MQ=255)

                                         gagGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCTTGAgcgcgcc                                                                                                              <  1:4751241/150‑2 (MQ=255)

                                           ggTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCTTGAgcgcgccaa                                                                                                            <  1:1229361/150‑4 (MQ=255)

                                           ggTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCTTGAgcgcgccaa                                                                                                            <  2:5075370/150‑4 (MQ=255)

                                            gTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGa                                                                                                                                      >  2:3624523/1‑123 (MQ=255)

                                            gTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCa                                                                                                           <  1:5065702/150‑1 (MQ=255)

                                            gTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCa                                                                                                           <  1:5118784/150‑1 (MQ=255)

                                            gTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCa                                                                                                           >  1:2160330/1‑150 (MQ=255)

                                            gTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCa                                                                                                           >  1:2319/1‑150 (MQ=255)

                                                 tCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATgg                                                                                                                                            <  1:1937365/112‑1 (MQ=255)

                                                 tCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACg                                                                                                      >  2:4738735/1‑150 (MQ=255)

                                                  cTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACgg                                                                                                     <  1:365571/150‑1 (MQ=255)

                                                   ttCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGc                                                                                                    <  1:4024466/150‑1 (MQ=255)

                                                    gccTGGGCATCGGGGTGGCGAATGTCGATGACGCTCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGcc                                                                                                   <  2:5468018/149‑1 (MQ=255)

                                                      cTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAt                                                                                                 <  2:5008211/150‑1 (MQ=255)

                                                       tGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTACTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATg                                                                                                >  1:3497275/1‑150 (MQ=255)

                                                                gggTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGc                                                                                       >  1:3701402/1‑150 (MQ=255)

                                                                gggTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGc                                                                                       >  1:489662/1‑150 (MQ=255)

                                                                gggTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGc                                                                                       <  1:2402916/150‑1 (MQ=255)

                                                                  gTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCtt                                                                                     <  2:2964258/150‑1 (MQ=255)

                                                                   tGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCttt                                                                                    >  2:5422791/1‑150 (MQ=255)

                                                                       gAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttctt                                                                                >  2:1905276/1‑150 (MQ=255)

                                                                       gAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttctt                                                                                >  2:1139158/1‑150 (MQ=255)

                                                                        aaTGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttcttc                                                                               <  2:2764458/150‑1 (MQ=255)

                                                                        aaTGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttcttc                                                                               <  2:3056309/150‑1 (MQ=255)

                                                                                   cGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGc                                                                    <  2:3500146/150‑1 (MQ=255)

                                                                                    gATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCCGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCTAGAGCGCGCTCCAGCACGGCCAGGTCGAACTGCTTTTCTTCGTGCTCCATGCg                                                                   >  1:3404217/1‑150 (MQ=255)

                                                                                    gATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGc                                                                                                    >  2:3844291/1‑117 (MQ=255)

                                                                                    gATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCg                                                                   >  1:220137/1‑150 (MQ=255)

                                                                                              ggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTg                                                         <  1:3303283/150‑1 (MQ=255)

                                                                                                 aGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCa                                                      >  1:5270120/1‑150 (MQ=255)

                                                                                                 aGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCa                                                      >  1:3777583/1‑150 (MQ=255)

                                                                                                  gcgcCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCAt                                                     <  2:3410414/150‑1 (MQ=255)

                                                                                                     ccTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATggg                                                  >  2:2032808/1‑150 (MQ=255)

                                                                                                       tCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGggtg                                                >  2:1729202/1‑150 (MQ=255)

                                                                                                       tCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGggtg                                                <  2:3319424/150‑1 (MQ=255)

                                                                                                          gCCACTTCCTCGATTTCGATGTCCGTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTc                                             <  2:5296119/150‑1 (MQ=255)

                                                                                                            cACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCgg                                           <  1:5336439/150‑1 (MQ=255)

                                                                                                             aCTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCggg                                          <  1:3478951/150‑1 (MQ=255)

                                                                                                             aCTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCggg                                          >  1:14072/1‑150 (MQ=255)

                                                                                                              cTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCAGGGGTGGTCGGGt                                         >  2:3167156/1‑150 (MQ=255)

                                                                                                               ttCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGtt                                        >  1:2409521/1‑150 (MQ=255)

                                                                                                                  cTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAc                                     >  2:2552704/1‑150 (MQ=255)

                                                                                                                   tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                    >  2:4112734/1‑150 (MQ=255)

                                                                                                                   tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                    >  1:1023674/1‑150 (MQ=255)

                                                                                                                   tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                    <  1:3623523/150‑1 (MQ=255)

                                                                                                                   tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                    >  1:5569802/1‑150 (MQ=255)

                                                                                                                     gATTTCGCTGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCACGAGCGCGCTCCAGCCCGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCga                                  <  2:2256631/150‑1 (MQ=255)

                                                                                                                     gATTTCGATGTCCTTGCCCAGCTCATCGATCACCTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCga                                  <  2:2080402/150‑1 (MQ=255)

                                                                                                                     gATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCga                                  >  2:1968123/1‑150 (MQ=255)

                                                                                                                        ttCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGAt                               >  2:5133575/1‑150 (MQ=255)

                                                                                                                           gATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAc                            >  2:4659076/1‑150 (MQ=255)

                                                                                                                            aTGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcg                           <  2:4698061/150‑1 (MQ=255)

                                                                                                                             tGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcgc                          >  2:3880697/1‑150 (MQ=255)

                                                                                                                               tCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcgccg                        <  1:2789802/150‑1 (MQ=255)

                                                                                                                                     cccAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTAt                  >  2:539802/1‑150 (MQ=255)

                                                                                                                                      ccAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCGTGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTAtt                 >  2:183173/1‑150 (MQ=255)

                                                                                                                                      ccAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTAtt                 >  1:5426666/1‑150 (MQ=255)

                                                                                                                                         gCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCg              >  1:1294387/1‑150 (MQ=255)

                                                                                                                                           ccATCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGc            >  1:681004/2‑150 (MQ=255)

                                                                                                                                            cATCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCa           >  1:1999395/1‑150 (MQ=255)

                                                                                                                                            cATCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCa           >  2:5085708/1‑150 (MQ=255)

                                                                                                                                            cATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCa           >  2:492411/1‑150 (MQ=255)

                                                                                                                                             aTCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt          >  1:3903899/1‑150 (MQ=255)

                                                                                                                                             aTCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt          >  1:5150744/1‑150 (MQ=255)

                                                                                                                                             aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt          >  1:1524252/1‑150 (MQ=255)

                                                                                                                                             aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt          <  1:4471998/150‑1 (MQ=255)

                                                                                                                                             aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt          >  1:2977127/1‑150 (MQ=255)

                                                                                                                                             aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt          >  1:1702667/1‑150 (MQ=255)

                                                                                                                                              tCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAtg         >  2:2415628/1‑150 (MQ=255)

                                                                                                                                              tCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAtg         >  2:5239537/1‑150 (MQ=255)

                                                                                                                                                    acaTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTg   <  2:1382349/150‑1 (MQ=255)

                                                                                                                                                    acaTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTg   <  2:727614/150‑1 (MQ=255)

                                                                                                                                                    acaTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTg   <  1:1777620/150‑1 (MQ=255)

                                                                                                                                                     caTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTgg  <  2:3701402/150‑1 (MQ=255)

                                                                                                                                                     caTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGATCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTgg  <  2:645428/150‑1 (MQ=255)

GTAGAACGGCATGGCCTGGACTTCGATACCTTCCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGG  >  NC_002947/5755254‑5755552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`