BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,755,435	A→T	5.2%	L378H (CTT→CAT)	thiI ←	tRNA sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,755,435	0	A	T	5.2%	971.5 / 24.2	295	L378H (CTT→CAT)	thiI	tRNA sulfurtransferase
Reads supporting (aligned to +/- strand): ref base A (153/122); new base T (9/7); total (162/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

CCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCGGTGGCCTGG  >  NC_002947/5755286‑5755584

  aGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAaga                                                                                                                                                     >  1:959394/1‑150 (MQ=255)

  aGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAaga                                                                                                                                                     <  2:108242/150‑1 (MQ=255)

  aGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAaga                                                                                                                                                     <  1:4834383/150‑1 (MQ=255)

    cACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgc                                                                                                                                                   <  2:122500/150‑1 (MQ=255)

     aCCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcg                                                                                                                                                  >  1:855621/1‑150 (MQ=255)

       cAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcgcg                                                                                                                                                >  1:210353/1‑150 (MQ=255)

        agagGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcgcgc                                                                                                                                               >  2:4230949/1‑150 (MQ=255)

         gagGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCTTGAgcgcgcc                                                                                                                                              <  1:4751241/150‑2 (MQ=255)

           ggTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCTTGAgcgcgccaa                                                                                                                                            <  2:5075370/150‑4 (MQ=255)

            gTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCa                                                                                                                                           >  1:2160330/1‑150 (MQ=255)

            gTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCa                                                                                                                                           <  1:5118784/150‑1 (MQ=255)

              tCGTCTTCCTGGGCATGGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCTTGAgcgcgccaagtt                                                                                                                                         <  1:7758/150‑7 (MQ=255)

                  cTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACgg                                                                                                                                     <  1:365571/150‑1 (MQ=255)

                  cTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACgg                                                                                                                                     <  2:2841524/150‑1 (MQ=255)

                    tCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGcc                                                                                                                                   <  2:1973921/150‑1 (MQ=255)

                    gccTGGGCATCGGCGTGGCGACTGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCAGCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGcc                                                                                                                                   <  2:5467854/149‑1 (MQ=255)

                     ccTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCa                                                                                                                                  >  1:1645507/1‑150 (MQ=255)

                     ccTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCa                                                                                                                                  >  1:4049990/1‑150 (MQ=255)

                     ccTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCa                                                                                                                                  <  1:3878322/150‑1 (MQ=255)

                       tGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATg                                                                                                                                >  1:3496879/1‑150 (MQ=255)

                       tGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATg                                                                                                                                >  2:4730398/1‑150 (MQ=255)

                        gggCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGt                                                                                                                               <  1:3441688/150‑1 (MQ=255)

                              cGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACt                                                                                                                         <  2:2342275/150‑1 (MQ=255)

                                gggTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGc                                                                                                                       <  1:2402916/150‑1 (MQ=255)

                                gggTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGc                                                                                                                       >  1:489662/1‑150 (MQ=255)

                                   tGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCttt                                                                                                                    >  2:5422627/1‑150 (MQ=255)

                                     gCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttc                                                                                                                  <  2:294360/150‑1 (MQ=255)

                                       gAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttctt                                                                                                                >  2:1905276/1‑150 (MQ=255)

                                        aaTGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAATGGAACTTGGCGCGCTCACGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttcttc                                                                                                               <  1:3324989/150‑1 (MQ=255)

                                        aaTGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttcttc                                                                                                               <  2:2764458/150‑1 (MQ=255)

                                        aaTGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTttcttc                                                                                                               <  2:3056309/150‑1 (MQ=255)

                                          tGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGt                                                                                                             >  2:3863676/1‑150 (MQ=255)

                                            tCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGc                                                                                                           <  1:3724917/150‑1 (MQ=255)

                                                   cGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGc                                                                                                    <  2:3939372/150‑1 (MQ=255)

                                                    gATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGc                                                                                                                                    <  1:3844291/117‑1 (MQ=255)

                                                        tggcctggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGa                                                                                               >  1:1136641/1‑150 (MQ=255)

                                                          gcctggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACg                                                                                             <  2:2464157/150‑1 (MQ=255)

                                                           cctggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCTTGCTCCATGCGGTGACGc                                                                                            >  1:1724985/1‑150 (MQ=255)

                                                             tggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCtt                                                                                          >  1:3220232/1‑150 (MQ=255)

                                                                 aGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCa                                                                                      >  1:5270120/1‑150 (MQ=255)

                                                                 aGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCa                                                                                      >  2:498296/1‑150 (MQ=255)

                                                                  gcgcCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCAt                                                                                     <  2:3410414/150‑1 (MQ=255)

                                                                     ccTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATggg                                                                                  >  2:2032808/1‑150 (MQ=255)

                                                                     ccTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATggg                                                                                  >  2:2544340/1‑150 (MQ=255)

                                                                     ccTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATggg                                                                                  >  2:1567963/1‑150 (MQ=255)

                                                                      cTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGggt                                                                                 <  2:4680896/150‑1 (MQ=255)

                                                                      cTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGggt                                                                                 <  1:1962166/150‑1 (MQ=255)

                                                                       tCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGggtg                                                                                <  2:3319424/150‑1 (MQ=255)

                                                                          gCCACTTCCTCGATTTCGATGTCCGTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTc                                                                             <  2:5296119/150‑1 (MQ=255)

                                                                            cACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCgg                                                                           <  1:5336439/150‑1 (MQ=255)

                                                                             aCTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCggg                                                                          >  1:14072/1‑150 (MQ=255)

                                                                               ttCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGtt                                                                        >  1:2409521/1‑150 (MQ=255)

                                                                                   tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                                                    >  1:5569802/1‑150 (MQ=255)

                                                                                   tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                                                    >  2:4112734/1‑150 (MQ=255)

                                                                                   tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                                                    >  1:2782078/1‑150 (MQ=255)

                                                                                   tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                                                    <  2:1334468/150‑1 (MQ=255)

                                                                                   tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                                                    >  1:1023674/1‑150 (MQ=255)

                                                                                     gATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCga                                                                  <  2:1634791/150‑1 (MQ=255)

                                                                                        ttCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGAt                                                               >  2:5133575/1‑150 (MQ=255)

                                                                                                 cTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCa                                                      >  2:5508082/1‑150 (MQ=255)

                                                                                                  ttGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAg                                                     >  1:1518845/1‑150 (MQ=255)

                                                                                                      ccAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTAtt                                                 >  2:183352/1‑150 (MQ=255)

                                                                                                         gCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCg                                              >  1:1294168/1‑150 (MQ=255)

                                                                                                           ccATCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGc                                            >  1:681004/2‑150 (MQ=255)

                                                                                                            cATCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCa                                           >  2:5085708/1‑150 (MQ=255)

                                                                                                            cATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCa                                           <  2:2966362/150‑1 (MQ=255)

                                                                                                             aTCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt                                          >  1:5150744/1‑150 (MQ=255)

                                                                                                             aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt                                          >  1:2977127/1‑150 (MQ=255)

                                                                                                             aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt                                          <  2:61371/150‑1 (MQ=255)

                                                                                                             aTCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAt                                          <  1:4471998/150‑1 (MQ=255)

                                                                                                              tCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAtg                                         >  2:2415628/1‑150 (MQ=255)

                                                                                                              tCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCAtg                                         >  1:2320727/1‑150 (MQ=255)

                                                                                                                 aTCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGc                                      <  1:361585/150‑1 (MQ=255)

                                                                                                                   cacaTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCtt                                    <  2:3978629/150‑1 (MQ=255)

                                                                                                                    acaTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTg                                   <  1:1777620/150‑1 (MQ=255)

                                                                                                                    acaTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTg                                   <  2:727614/150‑1 (MQ=255)

                                                                                                                        gATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCaa                               >  2:5280811/1‑150 (MQ=255)

                                                                                                                         aTCGATGGAACTGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCaaa                              <  2:2913859/150‑1 (MQ=255)

                                                                                                                         aTCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCaaa                              <  1:2032852/150‑1 (MQ=255)

                                                                                                                             aTGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGTAAAGTcc                          >  2:2258824/1‑150 (MQ=255)

                                                                                                                             aTGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTcc                          >  1:1874457/1‑150 (MQ=255)

                                                                                                                              tGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCcg                         <  2:487585/150‑1 (MQ=255)

                                                                                                                                gAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCcgcg                       <  1:5419868/150‑1 (MQ=255)

                                                                                                                                gAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCcgcg                       >  1:2541664/1‑150 (MQ=255)

                                                                                                                                 aaaTGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCgg                      <  2:2160330/150‑1 (MQ=255)

                                                                                                                                  aaTGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGt                     >  2:1584484/1‑150 (MQ=255)

                                                                                                                                       aCTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGa                <  1:4220805/150‑1 (MQ=255)

                                                                                                                                        tggagcgcgcTCTTGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGAt               >  1:550254/5‑150 (MQ=255)

                                                                                                                                          gagcgcgcTCTTGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGAttt             >  2:3823205/3‑150 (MQ=255)

                                                                                                                                          gagcgcgcTCTTGAGCGCGCTCCAGCACGGCCATGTCGAACTGCGTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGACGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTCCCGaggt             >  2:4642272/3‑147 (MQ=255)

                                                                                                                                            gcgcgcTCTTGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCaaa                              <  2:1767118/131‑1 (MQ=255)

                                                                                                                                            gcgcgcTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCg           <  1:3789158/150‑1 (MQ=255)

                                                                                                                                            gcgcgcTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCg           <  2:447178/150‑1 (MQ=255)

                                                                                                                                            gcgcgcTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCg           <  1:393004/150‑1 (MQ=255)

                                                                                                                                            gcgcgcTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCg           <  2:5602464/150‑1 (MQ=255)

                                                                                                                                             cgcgcTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCgg          <  2:210353/150‑1 (MQ=255)

                                                                                                                                              gcgcTCACGAGCGCGCTCCAGCACGGCCAAGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCACAGTCCGCGGTACCGATTTCGGt         <  2:3634955/150‑1 (MQ=255)

                                                                                                                                               cgcTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCCTGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCGGTg        <  2:3209836/150‑1 (MQ=255)

                                                                                                                                                  tCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCGGTGGcc     >  2:368313/1‑150 (MQ=255)

                                                                                                                                                  tCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGAGCTCCATGCGGTGACGCTTGGCAGGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGCTTTCGGTGccc     >  2:3471225/1‑150 (MQ=255)

CCAGCACCAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCATGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCGGTGGCCTGG  >  NC_002947/5755286‑5755584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`