BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,794,028	C→A	5.7%	C60* (TGC→TGA) ‡	ynfM →	efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,794,028	0	C	A	5.7%	745.0 / 22.4	220	C60* (TGC→TGA) ‡	ynfM	efflux transporter
Reads supporting (aligned to +/- strand): ref base C (117/90); new base A (10/3); total (127/93)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

CCCGTGAAAACTGCAGTAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACGCAAGCCCGTGA  >  NC_002947/5793879‑5794176

        aaCTGCAGTAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGcc                                                                                                                                              <  1:2503965/150‑1 (MQ=255)

        aaCTGCAGGAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGcc                                                                                                                                              <  1:2562582/150‑1 (MQ=255)

          cTGCAGTAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACGCAGTACAGcaag                                                                                                                                            <  2:1000775/150‑4 (MQ=255)

           tGCAGTAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCgat                                                                                                                                           >  1:3293567/1‑150 (MQ=255)

           tGCAGTAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCgat                                                                                                                                           >  1:2091449/1‑150 (MQ=255)

             cAGTAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCgatga                                                                                                                                         <  2:1220413/150‑1 (MQ=255)

             cAGTAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATCAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCgatga                                                                                                                                         <  2:378534/150‑1 (MQ=255)

              aGTAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCgatgat                                                                                                                                        <  1:3367751/150‑1 (MQ=255)

              aGTAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCAAGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCgatgat                                                                                                                                        >  1:3571313/1‑150 (MQ=255)

              aGTAGCCCCCCTCCTCCTTGAGCCAAAACCAGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCgatgat                                                                                                                                        <  2:5627979/150‑1 (MQ=255)

               gTAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGatgatg                                                                                                                                       >  2:5183843/1‑150 (MQ=255)

                tAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGc                                                                                                                                      <  2:1955949/150‑1 (MQ=255)

                tAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGc                                                                                                                                      >  1:4424383/1‑150 (MQ=255)

                   ccccccTCCTCCTTGAGCCAAAGCCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCctc                                                                                                                                   <  1:305555/150‑1 (MQ=255)

                   ccccccTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCctc                                                                                                                                   >  2:1835898/1‑150 (MQ=255)

                   ccccccTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCG‑AAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCctct                                                                                                                                  <  1:4892380/150‑1 (MQ=255)

                    cccccTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCctct                                                                                                                                  >  2:1646848/1‑150 (MQ=255)

                      cccTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTctgc                                                                                                                                >  1:1874468/1‑150 (MQ=255)

                      cccTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTctgc                                                                                                                                >  1:3948015/1‑150 (MQ=255)

                       cctcctcctTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTctgct                                                                                                                               >  2:1458614/1‑150 (MQ=255)

                          cctcctTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTc                                                                                                                            >  1:2358155/1‑150 (MQ=255)

                           ctcctTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTcc                                                                                                                           <  1:2496043/150‑1 (MQ=255)

                           ctcctTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTcc                                                                                                                           >  1:513223/1‑150 (MQ=255)

                           ctcctTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTcc                                                                                                                           <  2:3400143/150‑1 (MQ=255)

                             cctTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCg                                                                                                                         <  1:3041523/150‑1 (MQ=255)

                               ttGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCggg                                                                                                                       <  1:274244/150‑1 (MQ=255)

                                tGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGa                                                                                                                      <  1:5598454/150‑1 (MQ=255)

                                tGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGa                                                                                                                      <  1:1257575/150‑1 (MQ=255)

                                   gCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGtt                                                                                                                   >  1:615889/1‑150 (MQ=255)

                                    ccACAACCTGCCATCCTGACCGAAATGTGGATCGAAAAATGCACACCCGCCTTCATGATAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGttt                                                                                                                  <  2:3010602/150‑1 (MQ=255)

                                    ccAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGttt                                                                                                                  >  1:3143802/1‑150 (MQ=255)

                                    ccAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGttt                                                                                                                  >  2:5588829/1‑150 (MQ=255)

                                    ccAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGttt                                                                                                                  <  1:4368276/150‑1 (MQ=255)

                                    ccAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGttt                                                                                                                  >  1:5385358/1‑150 (MQ=255)

                                     cAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTg                                                                                                                 >  1:787036/1‑150 (MQ=255)

                                      aaaaCCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGc                                                                                                                >  2:311264/1‑150 (MQ=255)

                                      aaaaCCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGc                                                                                                                >  2:3316913/1‑150 (MQ=255)

                                      aaaaCCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGc                                                                                                                >  2:1962964/1‑150 (MQ=255)

                                      aaaaCCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGc                                                                                                                <  2:1156779/150‑1 (MQ=255)

                                      aaaaCCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGc                                                                                                                >  2:529788/1‑150 (MQ=255)

                                                aTCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAAcgcg                                                                                                      <  1:3626086/150‑1 (MQ=255)

                                                     gACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCGTCAACGCGGcaca                                                                                                 <  2:4030100/150‑1 (MQ=255)

                                                      aCCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTTAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACag                                                                                                >  2:4497656/1‑150 (MQ=255)

                                                      aCCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACag                                                                                                >  1:5084864/1‑150 (MQ=255)

                                                       ccGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACaga                                                                                               <  1:2893129/150‑1 (MQ=255)

                                                        cGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACagag                                                                                              >  1:5553770/1‑150 (MQ=255)

                                                         gAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGagc                                                                                             >  2:4365074/1‑150 (MQ=255)

                                                          aaaTGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGagca                                                                                            <  1:1835898/150‑1 (MQ=255)

                                                                ggATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTgg                                                                                      >  1:3100791/1‑150 (MQ=255)

                                                                 gATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGt                                                                                     >  2:3263592/1‑150 (MQ=255)

                                                                 gATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGt                                                                                     <  1:365598/150‑1 (MQ=255)

                                                                    tcgaaaaGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCt                                                                                  >  1:3161544/4‑150 (MQ=255)

                                                                    cGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCt                                                                                  >  1:3196457/1‑150 (MQ=255)

                                                                      aaaaaGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACaga                                                                                               >  2:3045618/1‑135 (MQ=255)

                                                                        aaaGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCg                                                                              <  1:279446/150‑1 (MQ=255)

                                                                          aGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGgt                                                                            <  1:5530461/150‑1 (MQ=255)

                                                                          aGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGgt                                                                            <  1:1458614/150‑1 (MQ=255)

                                                                            gCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGgtgt                                                                          >  1:4199039/1‑150 (MQ=255)

                                                                            gCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGgtgt                                                                          >  2:2564498/1‑150 (MQ=255)

                                                                            gCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGgtgt                                                                          >  1:4616583/1‑150 (MQ=255)

                                                                             cacaCCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTc                                                                         <  2:5237529/150‑1 (MQ=255)

                                                                             cacaCCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTc                                                                         <  1:1962436/150‑1 (MQ=255)

                                                                                  ccGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCg                                                                    >  1:4925907/1‑150 (MQ=255)

                                                                                        tCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGc                                                              >  2:3938644/1‑150 (MQ=255)

                                                                                        tCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGc                                                              <  1:2465157/150‑1 (MQ=255)

                                                                                               aaCCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGtt                                                       <  1:3932272/150‑1 (MQ=255)

                                                                                                aCCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTc                                                      <  1:3207249/150‑1 (MQ=255)

                                                                                                 ccGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCg                                                     <  2:4710773/150‑1 (MQ=255)

                                                                                                       tGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCATAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCctgc                                               >  1:1412950/1‑150 (MQ=255)

                                                                                                           cTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTc                                                                         >  1:3859108/1‑120 (MQ=255)

                                                                                                           cTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTCTCCACCGCCATGCTGGCGTTCGGCCTGCTGAt                                           >  2:5538816/1‑150 (MQ=255)

                                                                                                            ttGTTCAGCGGCGGTTTCGCGACCTTGGCCTAGCTGTACTGCGTACAGCCGATCATGCCTCTGCTGTCCCGGGAGTATGCCATCAACGCGCCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCAGATc                                          >  2:503362/1‑150 (MQ=255)

                                                                                                            ttGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATc                                          >  2:75966/1‑150 (MQ=255)

                                                                                                              gTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCAc                                        <  1:5618796/150‑1 (MQ=255)

                                                                                                                tCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCg                                      >  1:4480168/1‑150 (MQ=255)

                                                                                                                  aGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTCGCCATCAACGCGGcaca                                                                                                 >  2:4568153/1‑89 (MQ=255)

                                                                                                                   gcggcgGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGcc                                   >  2:4835892/1‑150 (MQ=255)

                                                                                                                    cggcggTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGccc                                  >  1:1294056/1‑150 (MQ=255)

                                                                                                                        ggTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCAtt                              >  2:2119549/1‑150 (MQ=255)

                                                                                                                         gTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCAttt                             <  1:5335253/150‑1 (MQ=255)

                                                                                                                             cGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGa                         <  2:1346368/150‑1 (MQ=255)

                                                                                                                              gCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGAc                        >  1:471113/1‑150 (MQ=255)

                                                                                                                              gCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGAc                        >  2:3747396/1‑150 (MQ=255)

                                                                                                                                 aCCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGc                     >  1:2744890/1‑150 (MQ=255)

                                                                                                                                    ttCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATc                  >  2:4682572/1‑150 (MQ=255)

                                                                                                                                       catcggCTGTACGCAGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGa               >  1:5452487/6‑150 (MQ=255)

                                                                                                                                        ccTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGAc              <  1:5611678/150‑1 (MQ=255)

                                                                                                                                        ccTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGAc              <  1:1091236/150‑1 (MQ=255)

                                                                                                                                         tcggCTGTACGCAGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATGTCTGACCGCATCGGACg             >  2:5373749/4‑150 (MQ=255)

                                                                                                                                         cTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACg             >  2:27359/1‑150 (MQ=255)

                                                                                                                                           ggCTGTACGCAGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACGCa           >  1:2980873/2‑150 (MQ=255)

                                                                                                                                           ggCTGTACGCAGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACGCa           >  2:2346241/2‑150 (MQ=255)

                                                                                                                                            gCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACGCaa          <  1:5198901/150‑1 (MQ=255)

                                                                                                                                            gCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACGCaa          <  1:3263592/150‑1 (MQ=255)

                                                                                                                                            gCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACGCaa          >  2:3139175/1‑150 (MQ=255)

                                                                                                                                            gCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACGCaa          <  1:2009120/150‑1 (MQ=255)

                                                                                                                                            gCTGTACGCAGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACGCaa          >  1:2877034/1‑150 (MQ=255)

                                                                                                                                                tACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACGCAAGccc      >  2:4084582/1‑150 (MQ=255)

                                                                                                                                                  cTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACGCAAGCCCGt    >  2:2429847/1‑150 (MQ=255)

                                                                                                                                                    gCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACGCAAGCCCGTGa  <  2:3019731/150‑1 (MQ=255)

CCCGTGAAAACTGCAGTAGCCCCCCTCCTCCTTGAGCCAAAACCTGCCATCCTGACCGAAATGTGGATCGAAAAAGGCACACCCGCCTTCATGAAAACCGTGCTGGCCTTGTTCAGCGGCGGTTTCGCCACCTTCGCCTTGCTGTACTGCGTACAGCCGATGATGCCTCTGCTGTCCCGGGAGTTTGCCATCAACGCGGCACAGAGCAGCCTGGTGCTGTCGGTGTCCACCGCCATGCTGGCGTTCGGCCTGCTGATCACCGGCCCCATTTCTGACCGCATCGGACGCAAGCCCGTGA  >  NC_002947/5793879‑5794176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`