BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	537,454	T→C	5.7%	noncoding (47/66 nt)	PP_mr07 →	P26 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	537,454	0	T	C	5.7%	791.7 / 22.4	244	noncoding (47/66 nt)	PP_mr07	P26 ncRNA
Reads supporting (aligned to +/- strand): ref base T (105/125); new base C (8/6); total (113/131)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 256 total aligned reads displayed.

ATGGCGCTCCTCAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAG  >  NC_002947/537305‑537603

 tGGCGCTCCTCAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCtg                                                                                                                                                      >  2:1389110/1‑150 (MQ=255)

   gcgcTCCTCAGGTTGTAGCTGAAGGCGTTTCGACAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCtggt                                                                                                                                                    <  2:261120/150‑1 (MQ=255)

     gcTCCTCAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCtggtgg                                                                                                                                                  >  2:2565938/1‑150 (MQ=255)

        cctcAGGTTGTAGATGAAGGCTTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTTAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCtt                                                                                                                                               <  1:1100629/150‑1 (MQ=255)

         ctcAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTa                                                                                                                                              >  1:765404/1‑150 (MQ=255)

          tcAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTAt                                                                                                                                             >  2:104556/1‑150 (MQ=255)

          tcAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTAt                                                                                                                                             >  2:3271005/1‑150 (MQ=255)

            aGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGc                                                                                                                                           <  2:5600062/150‑1 (MQ=255)

              gTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCa                                                                                                                                         <  1:2237347/150‑1 (MQ=255)

                 ttAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGACGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCCGGTGGCATAAGCCAcca                                                                                                                                      <  1:2705271/149‑2 (MQ=255)

                   aGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGc                                                                                                                                    >  1:2635290/1‑150 (MQ=255)

                    gCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCCGGTGGCATAAGCCACCAGcc                                                                                                                                   <  2:1126108/150‑1 (MQ=255)

                        aaGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCtttt                                                                                                                               >  2:4512255/1‑150 (MQ=255)

                          ggCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTc                                                                                                                             <  1:4679118/150‑1 (MQ=255)

                           gCGTTTCGAAAGATGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTcc                                                                                                                            <  1:5438715/150‑1 (MQ=255)

                           gCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTcc                                                                                                                            <  1:2105433/150‑1 (MQ=255)

                           gCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCCGGTGGCATAAGCCACCAGccatcagcc                                                                                                                            <  2:756749/150‑8 (MQ=255)

                                tCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTAt                                                                                                                       <  1:50638/150‑1 (MQ=255)

                                 cGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTAtt                                                                                                                      <  2:5280360/150‑1 (MQ=255)

                                  gAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATtg                                                                                                                     >  1:4816751/1‑150 (MQ=255)

                                  gAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTGTTTCCGTTATtg                                                                                                                     >  2:1524318/1‑150 (MQ=255)

                                   aaaGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATtgg                                                                                                                    >  1:5605788/1‑150 (MQ=255)

                                   aaaGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATtgg                                                                                                                    >  1:426973/1‑150 (MQ=255)

                                    aagaagCCGCTGAAGACGCTAAGAAGAAGCCGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATtggt                                                                                                                   <  1:4509093/150‑1 (MQ=255)

                                           cGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGt                                                                                                            >  1:2799779/1‑150 (MQ=255)

                                                aaGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGc                                                                                                       <  2:4887340/150‑1 (MQ=255)

                                                  gACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCg                                                                                                     >  2:3052613/1‑150 (MQ=255)

                                                  gACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCg                                                                                                     <  2:4010128/150‑1 (MQ=255)

                                                      cTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTcc                                                                                                 >  1:1286740/1‑150 (MQ=255)

                                                       tAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTAtt                                                                                                                      >  2:4613837/1‑128 (MQ=255)

                                                       tAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTccc                                                                                                >  1:484385/1‑150 (MQ=255)

                                                       tAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTccc                                                                                                >  1:607735/1‑150 (MQ=255)

                                                        aagaagaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCg                                                                                               >  2:3450446/1‑150 (MQ=255)

                                                          gaagaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGAt                                                                                             <  1:4239704/150‑1 (MQ=255)

                                                           aagaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATa                                                                                            <  2:2309306/150‑1 (MQ=255)

                                                               agCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGa                                                                                        <  2:3986832/150‑1 (MQ=255)

                                                                gCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAg                                                                                       >  2:2780989/1‑150 (MQ=255)

                                                                 cTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGc                                                                                      <  2:533887/150‑1 (MQ=255)

                                                                  tGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCt                                                                                                                                  <  2:3296439/105‑1 (MQ=255)

                                                                    gaagaaGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGc                                                                                   >  2:277170/1‑150 (MQ=255)

                                                                    gaagaaGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGc                                                                                   <  1:70967/150‑1 (MQ=255)

                                                                    gaagaaGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGc                                                                                   >  2:2494152/1‑150 (MQ=255)

                                                                      agaagCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCaa                                                                                 <  2:920058/150‑1 (MQ=255)

                                                                      agaagCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCaa                                                                                 <  2:4816751/150‑1 (MQ=255)

                                                                      agaagCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCaa                                                                                 <  1:4942946/150‑1 (MQ=255)

                                                                        aagCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGa                                                                               <  1:3005532/150‑1 (MQ=255)

                                                                          gCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGaca                                                                             <  1:1832364/150‑1 (MQ=255)

                                                                           cAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGacac                                                                            <  2:343828/150‑1 (MQ=255)

                                                                                gcTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTCGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCAtg                                                                       >  1:3809651/1‑150 (MQ=255)

                                                                                 cTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCAtgt                                                                      <  1:1060468/150‑1 (MQ=255)

                                                                                 cTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCAtgt                                                                      >  2:3800248/1‑150 (MQ=255)

                                                                                    aaGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATgtggg                                                                   >  2:2140382/1‑150 (MQ=255)

                                                                                    aaGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATgtggg                                                                   >  1:327948/1‑150 (MQ=255)

                                                                                     aGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATgtgggt                                                                  <  1:1873422/150‑1 (MQ=255)

                                                                                      gTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATgtgggtg                                                                 <  1:2484355/150‑1 (MQ=255)

                                                                                         gAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGgcg                                                              <  1:3904011/150‑1 (MQ=255)

                                                                                          aGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGgcgc                                                             <  2:4312710/150‑1 (MQ=255)

                                                                                            cTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCaa                                                           >  1:4310689/1‑150 (MQ=255)

                                                                                             tGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCaaa                                                          >  1:3715850/1‑150 (MQ=255)

                                                                                               aaGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAAcc                                                        >  1:845268/1‑150 (MQ=255)

                                                                                                  tAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAg                                                     <  1:5470131/150‑1 (MQ=255)

                                                                                                    aTCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAggg                                                   <  1:4651389/150‑1 (MQ=255)

                                                                                                     tctcGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAgggg                                                  >  1:1673501/1‑150 (MQ=255)

                                                                                                      ctcGACGTTGCGATGACAGCCTGAGCGTTGAGCGATAGGCTGATGGCTGGTGTCTTATGCCACCGGACTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGTTGGCGCAAACATAGGGGt                                                 <  2:123175/150‑1 (MQ=255)

                                                                                                      ctcGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGt                                                 <  2:3757468/150‑1 (MQ=255)

                                                                                                       tcGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGtt                                                >  2:821823/1‑150 (MQ=255)

                                                                                                       tcGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGtt                                                <  1:3563042/150‑1 (MQ=255)

                                                                                                            cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAggg                                                   >  1:2922376/1‑142 (MQ=255)

                                                                                                            cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCAc                                           >  1:4045367/1‑150 (MQ=255)

                                                                                                            cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCAc                                           >  2:2906301/1‑150 (MQ=255)

                                                                                                            cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCAc                                           >  2:60912/1‑150 (MQ=255)

                                                                                                            cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCAc                                           >  1:4045497/1‑150 (MQ=255)

                                                                                                            cTTCCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCAc                                           >  1:2391870/1‑150 (MQ=255)

                                                                                                             ttGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACg                                          >  1:290924/1‑150 (MQ=255)

                                                                                                              tGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGa                                         <  2:635519/150‑1 (MQ=255)

                                                                                                                 gATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGAttt                                      >  1:1435914/1‑150 (MQ=255)

                                                                                                                  aTGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGGGGGTGGCGCAAACCAAGGGGTTTGCACGAtttt                                     >  1:4091867/1‑150 (MQ=255)

                                                                                                                      cAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTc                                                                                                           >  1:1336831/1‑76 (MQ=255)

                                                                                                                        gCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGgct                               >  1:934183/1‑150 (MQ=255)

                                                                                                                          cTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCAGGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGgctgc                             <  2:3235099/150‑1 (MQ=255)

                                                                                                                              gCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTccc                         <  1:411266/150‑1 (MQ=255)

                                                                                                                                  tGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCccgccg                     <  1:1605997/150‑1 (MQ=255)

                                                                                                                                   gAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg                    <  1:3424000/150‑1 (MQ=255)

                                                                                                                                        aaaGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa               <  1:5552005/150‑1 (MQ=255)

                                                                                                                                        aaaGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa               <  2:4414777/150‑1 (MQ=255)

                                                                                                                                          aGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGagcagc             <  2:2581742/150‑1 (MQ=255)

                                                                                                                                          aGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGagcagc             <  2:1937467/150‑1 (MQ=255)

                                                                                                                                          aGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGagcagc             <  1:3052613/150‑1 (MQ=255)

                                                                                                                                           ggCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGcc            <  2:2218627/150‑1 (MQ=255)

                                                                                                                                            gCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGAGCAGCCa           <  1:3248777/150‑1 (MQ=255)

                                                                                                                                                aaggCCGGTGGCATAAGCCACCAGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACa       >  1:812868/3‑150 (MQ=255)

                                                                                                                                                 aggCCGGTGGCATAAGCCACCAGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCAt                                                                        <  1:5113476/83‑1 (MQ=38)

                                                                                                                                                  ggCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAg     <  1:5319596/150‑1 (MQ=255)

                                                                                                                                                  ggCCGGTGGCATAAGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAg     >  2:3630637/1‑150 (MQ=255)

                                                                                                                                                  ggCCGGTGGCATAAGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAg     >  2:1294866/1‑150 (MQ=255)

                                                                                                                                                  ggCCGGTGGCATAAGCCACCAGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAg     >  1:1987941/1‑150 (MQ=255)

                                                                                                                                                    cTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCACGACACCCATGTGGGTGGCGCAAACCAAGGGGTGTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCa   >  2:1806208/1‑150 (MQ=255)

                                                                                                                                                     tggtggCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAg  >  1:5226018/1‑150 (MQ=255)

                                                                                                                                                     tggtggCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAg  <  1:4552965/150‑1 (MQ=255)

                                                                                                                                                     tggtggCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAg  >  1:2574163/1‑150 (MQ=255)

ATGGCGCTCCTCAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAG  >  NC_002947/537305‑537603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`