Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,109,765 G→A 6.0% H567Y (CAC→TAC) ‡ PP_5355 ← sodium/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,109,7650GA 6.0% 941.8 / 29.5 297H567Y (CAC→TAC) ‡PP_5355sodium/proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (158/121);  new base A (11/7);  total (169/128)
Fisher's exact test for biased strand distribution p-value = 8.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

AAATCGCCAGGCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGATC  >  NC_002947/6109617‑6109910
                                                                                                                                                    |                                                                                                                                                 
aaaTCGCCAGGCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGAGTGc                                                                                                                                                  >  2:3086851/1‑150 (MQ=255)
     gCCAGGCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCAcgcg                                                                                                                                             >  1:1851680/1‑150 (MQ=255)
      ccAGGCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCgg                                                                                                                                            >  2:4719623/1‑150 (MQ=255)
       cAGGCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGa                                                                                                                                           >  1:2145135/1‑150 (MQ=255)
        aGGCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATCCGCGTGCACACGCGCAt                                                                                                                                          <  1:2528869/150‑1 (MQ=255)
         ggCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATg                                                                                                                                         >  1:2858363/1‑150 (MQ=255)
          gCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGa                                                                                                                                        <  1:4458906/150‑1 (MQ=255)
          gCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGa                                                                                                                                        <  1:2972048/150‑1 (MQ=255)
          gCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGa                                                                                                                                        <  2:3243154/150‑1 (MQ=255)
          gCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGa                                                                                                                                        <  1:2837589/150‑1 (MQ=255)
                   gcCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGcc                                                                                                                               >  2:4048324/1‑150 (MQ=255)
                   gcCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGcc                                                                                                                               >  2:1107285/1‑150 (MQ=255)
                    ccTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCca                                                                                                                              <  2:1012217/150‑1 (MQ=255)
                     cTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCcac                                                                                                                             <  1:1065467/150‑1 (MQ=255)
                             aTCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGc                                                                                                                     >  1:2288729/1‑150 (MQ=255)
                             aTCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGc                                                                                                                     >  1:2075720/1‑150 (MQ=255)
                             aTCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGc                                                                                                                     >  1:1952029/1‑150 (MQ=255)
                             aTCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGc                                                                                                                     >  1:4621774/1‑150 (MQ=255)
                                gCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGccac                                                                                                                  <  2:2972993/150‑1 (MQ=255)
                                  tataGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGccacca                                                                                                                >  1:2659385/1‑150 (MQ=255)
                                  tataGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGccacca                                                                                                                >  1:3070144/1‑150 (MQ=255)
                                   ataGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATCCGCGTGCACACGCGCATgcagat                                                                                                                                    <  2:1605959/129‑6 (MQ=255)
                                   ataGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATCCGCGTGCACACGCGCATgcagat                                                                                                                                    >  1:1605959/1‑124 (MQ=255)
                                   ataGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGACAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGccaccac                                                                                                               <  1:827362/150‑1 (MQ=255)
                                          cATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCa                                                                                                        <  2:5311067/150‑1 (MQ=255)
                                          cATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCa                                                                                                        <  2:3184129/150‑1 (MQ=255)
                                             ctcctcGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCt                                                                                                     <  1:3098909/150‑1 (MQ=255)
                                               cctcGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCGGCTAc                                                                                                   >  1:5327587/1‑150 (MQ=255)
                                               cctcGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTAc                                                                                                   <  2:4456245/150‑1 (MQ=255)
                                                 tcGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCa                                                                                                 >  1:4420589/1‑150 (MQ=255)
                                                 tcGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCa                                                                                                 >  1:4224368/1‑150 (MQ=255)
                                                 tcGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCa                                                                                                 >  2:2400239/1‑150 (MQ=255)
                                                    ggcgggcgAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCGGCGCCACAACAACGCCACCACCACCGCAGCTACCACgg                                                                                              <  1:478713/150‑1 (MQ=255)
                                                    ggcgggcgAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACgg                                                                                              <  2:5263531/150‑1 (MQ=255)
                                                        ggcgAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAAt                                                                                          <  2:2574001/150‑1 (MQ=255)
                                                           gagaTACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCAc                                                                                       >  2:3344649/1‑150 (MQ=255)
                                                           gagaTACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCAc                                                                                       >  1:538100/1‑150 (MQ=255)
                                                           gagaTACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCAc                                                                                       >  2:1094480/1‑150 (MQ=255)
                                                            agaTACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCAcc                                                                                      >  1:1023746/1‑150 (MQ=255)
                                                            agaTACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCAcc                                                                                      >  1:3167186/1‑150 (MQ=255)
                                                               tACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACCACGCCACCACCACCGCAGCTACCACGGCAATCACcagc                                                                                   <  2:5356895/150‑1 (MQ=255)
                                                                aCTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACcagca                                                                                  <  2:3863823/150‑1 (MQ=255)
                                                                aCTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACcagca                                                                                  <  1:919055/150‑1 (MQ=255)
                                                                 cTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACcagcag                                                                                 <  1:2603745/150‑1 (MQ=255)
                                                                  tttGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCagcagc                                                                                >  2:2879589/1‑150 (MQ=255)
                                                                       ccGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTc                                                                           >  1:428297/1‑150 (MQ=255)
                                                                       ccGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTc                                                                           >  1:4040424/1‑150 (MQ=255)
                                                                          gggCGGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCt                                                                        <  2:903683/150‑1 (MQ=255)
                                                                           ggCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCtg                                                                       <  2:4443237/150‑1 (MQ=255)
                                                                              aGACCCCCTCAGTGCGAGTTATCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTACACGCGGATGAGCCAGCGCCACAACAACTCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCtggtg                                                                    >  1:400949/1‑150 (MQ=255)
                                                                               gACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCtggtgg                                                                   >  1:5562106/1‑150 (MQ=255)
                                                                                  ccccTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCa                                                                >  2:3426197/1‑150 (MQ=255)
                                                                                  ccccTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCa                                                                >  2:2659532/1‑150 (MQ=255)
                                                                                   cccTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAg                                                               >  1:1353766/1‑150 (MQ=255)
                                                                                   cccTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAg                                                               >  1:4174028/1‑150 (MQ=255)
                                                                                     cTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGaa                                                             >  1:3806413/1‑150 (MQ=255)
                                                                                      tCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAAt                                                            <  2:5407043/150‑1 (MQ=255)
                                                                                       cAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATg                                                           <  1:3714161/150‑1 (MQ=255)
                                                                                         gTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCt                                                         <  1:3564778/150‑1 (MQ=255)
                                                                                           gCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTgg                                                       <  1:745203/150‑1 (MQ=255)
                                                                                            cGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGc                                                      >  2:5526804/1‑150 (MQ=255)
                                                                                             gAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCg                                                     >  2:5111255/1‑150 (MQ=255)
                                                                                                ttCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGAc                                                  >  1:3462329/1‑150 (MQ=255)
                                                                                                    cgcgACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATg                                              <  2:492552/150‑1 (MQ=255)
                                                                                                    cgcgACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATg                                              <  2:1579985/150‑1 (MQ=255)
                                                                                                     gcgACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGc                                             >  2:3100418/1‑150 (MQ=255)
                                                                                                      cgACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGcc                                            <  1:5407555/150‑1 (MQ=255)
                                                                                                      cgACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGcc                                            <  2:790947/150‑1 (MQ=255)
                                                                                                       gACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCg                                           >  1:2241379/1‑150 (MQ=255)
                                                                                                          tGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGTGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAca                                        >  2:3515567/1‑150 (MQ=255)
                                                                                                          tGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACGACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAca                                        >  2:3907324/1‑150 (MQ=255)
                                                                                                          tGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAca                                        >  2:3166286/1‑150 (MQ=255)
                                                                                                          tGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAca                                        >  2:4299595/1‑150 (MQ=255)
                                                                                                          tGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAca                                        <  1:2650016/150‑1 (MQ=255)
                                                                                                          tGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAca                                        >  2:2119627/1‑150 (MQ=255)
                                                                                                           gTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAcag                                       >  1:4950673/1‑150 (MQ=255)
                                                                                                               tcCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGTGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAcagcagc                                   >  2:62955/1‑150 (MQ=255)
                                                                                                               tcCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAcagcagc                                   >  2:1738616/1‑150 (MQ=255)
                                                                                                               tcCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAcagcagc                                   >  2:828279/1‑150 (MQ=255)
                                                                                                               tcCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAcagcagc                                   >  2:4611819/1‑150 (MQ=255)
                                                                                                               tcCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAcagcagc                                   >  2:2182695/1‑150 (MQ=255)
                                                                                                                   gCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGa                               >  2:1974988/1‑150 (MQ=255)
                                                                                                                     gTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGaaa                             <  2:3070144/150‑1 (MQ=255)
                                                                                                                         ccAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCa                         >  2:2221429/1‑150 (MQ=255)
                                                                                                                         ccAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCa                         >  2:1367451/1‑150 (MQ=255)
                                                                                                                          cAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCAc                        >  1:2914996/1‑150 (MQ=255)
                                                                                                                          cAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCAc                        <  1:4580543/150‑1 (MQ=255)
                                                                                                                          cAGCAACGCGATCTGCATGCGCGTGTACACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCAc                        >  1:3848306/1‑150 (MQ=255)
                                                                                                                           aGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCAcc                       <  1:1824364/150‑1 (MQ=255)
                                                                                                                                 gcgATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGc                 >  2:368975/1‑150 (MQ=255)
                                                                                                                                  tcaTCCGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCg                >  1:940830/3‑150 (MQ=255)
                                                                                                                                    aTCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGac              <  1:1896833/150‑1 (MQ=255)
                                                                                                                                       tGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGacaac           >  1:1698207/1‑150 (MQ=255)
                                                                                                                                       tGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGacaac           >  2:1748067/1‑150 (MQ=255)
                                                                                                                                       ctcATCCGCGTGCACACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAACTCACCAACAGCGacaac           >  1:4695172/3‑150 (MQ=255)
                                                                                                                                          aTGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGc        >  1:3974588/1‑150 (MQ=255)
                                                                                                                                           ttcgcgTGTGCTCGAGTATGATCCATAGCCACAACAACGCCACCACCACCGCAGCTTCCACGGGAATCACCAGCAGAAATTCGCTGGTGGGCAGAATGCTGGCGTACTATTCCGACAGCAGCAGGAAAATCACCAACCGCGACAACAGCg       <  2:4677207/148‑1 (MQ=255)
                                                                                                                                              gcgTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGa    >  1:5530210/1‑150 (MQ=255)
                                                                                                                                              gcgTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGa    >  1:5021628/1‑150 (MQ=255)
                                                                                                                                              gcgTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGa    <  1:3275961/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AAATCGCCAGGCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGATC  >  NC_002947/6109617‑6109910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑