BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	878,088	A→C	5.2%	F32V (TTC→GTC)	hprA ←	glycerate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	878,088	0	A	C	5.2%	823.2 / 23.6	240	F32V (TTC→GTC)	hprA	glycerate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (105/121); new base C (4/9); total (109/131)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 243 total aligned reads displayed.

GGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCATGC  >  NC_002947/877939‑878236

 gTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAAc                                                                                                                                                     <  2:3880353/150‑1 (MQ=255)

       gTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCg                                                                                                                                               <  1:15228/150‑1 (MQ=255)

          gcggcCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAAc                                                                                                                                            <  2:2610320/150‑1 (MQ=255)

               caccaGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGACCAGTTCGAActgttc                                                                                                                                       <  1:4538266/150‑4 (MQ=255)

               caccaGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGACCAGTTCGAActgttc                                                                                                                                       <  1:1822028/150‑4 (MQ=255)

                 ccaGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTgctgct                                                                                                                                     >  1:89798/1‑150 (MQ=255)

                 ccaGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTgctgct                                                                                                                                     >  2:192044/1‑150 (MQ=255)

                  caGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCtt                                                                                                                                    >  1:1649430/1‑150 (MQ=255)

                   aGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTc                                                                                                                                   <  2:3585238/150‑1 (MQ=255)

                      aTCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAgg                                                                                                                                <  2:3122134/150‑1 (MQ=255)

                       tCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAggg                                                                                                                               >  2:1656868/1‑150 (MQ=255)

                           cTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGa                                                                                                                           >  1:223419/1‑150 (MQ=255)

                           cTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGa                                                                                                                           >  2:3002576/1‑150 (MQ=255)

                           cTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGa                                                                                                                           >  2:1142764/1‑150 (MQ=255)

                               cgtggCGGGTTGGCGGCCAGGGGGGCGGCGTCGAGCAGGACCGTGGTGCTGACCACGGCCCCGGCGCCCTGCACGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGCACAGTTCGACCTGGTCGACCTGCGGCTTCAGGGGCGAAagg                                                                                                                       <  2:241002/146‑1 (MQ=255)

                                 ttGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtc                                                                                                                     <  1:3048183/150‑1 (MQ=255)

                                  tGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtcg                                                                                                                    <  2:3342054/150‑1 (MQ=255)

                                   gCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCACGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtcga                                                                                                                   <  2:3331880/150‑1 (MQ=255)

                                   gCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtcga                                                                                                                   <  2:5375405/150‑1 (MQ=255)

                                   gCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtcga                                                                                                                   <  2:1758163/150‑1 (MQ=255)

                                    cGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtcgag                                                                                                                  >  2:5480075/1‑150 (MQ=255)

                                     gggTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtcgagg                                                                                                                 >  1:5310002/1‑150 (MQ=255)

                                         tGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGc                                                                                                             >  2:4313984/1‑150 (MQ=255)

                                           gcggcCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGt                                                                                                                                                 <  1:4305743/112‑1 (MQ=255)

                                           gcggcCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGt                                                                                                                                                 >  2:4305743/1‑112 (MQ=255)

                                            cggcCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCaa                                                                                                          <  2:1431966/150‑1 (MQ=255)

                                             ggcCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCTCTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAg                                                                                                         <  2:2701809/150‑1 (MQ=255)

                                             ggcCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAg                                                                                                         >  2:1604553/1‑150 (MQ=255)

                                                     ttGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAggg                                                                                                 >  2:2972336/1‑150 (MQ=255)

                                                        cggcgTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGAtt                                                                                              <  2:2447118/150‑1 (MQ=255)

                                                         ggcgTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTg                                                                                             <  2:3195023/150‑1 (MQ=255)

                                                          gcgTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTgg                                                                                            <  1:105428/150‑1 (MQ=255)

                                                          gcgTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTgg                                                                                            <  1:343569/150‑1 (MQ=255)

                                                           cgTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAATTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGt                                                                                           <  1:5156355/150‑1 (MQ=255)

                                                             tCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGa                                                                                         <  2:4079262/150‑1 (MQ=255)

                                                               gAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATc                                                                                       <  1:102229/150‑1 (MQ=255)

                                                               gAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGCCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATc                                                                                       >  2:4022691/1‑150 (MQ=255)

                                                                 gCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCga                                                                                     <  2:3728713/150‑1 (MQ=255)

                                                                 gCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCga                                                                                     <  2:3009992/150‑1 (MQ=255)

                                                                   aTGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCgaga                                                                                   <  2:765316/150‑1 (MQ=255)

                                                                   aTGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCgaga                                                                                   <  2:3618112/150‑1 (MQ=255)

                                                                    tGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGaa                                                                                  <  1:1163993/150‑1 (MQ=255)

                                                                     gagcTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTGTGGGGCGAAAGGTCGAGGTCGCGAAGGTCCAGGGATTGGTGATCGAGaaa                                                                                 >  1:4565539/4‑150 (MQ=255)

                                                                     gACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGTGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtc                                                                                                                     <  1:3919398/114‑1 (MQ=255)

                                                                     gACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggta                                                                                                                     >  2:3919398/1‑113 (MQ=255)

                                                                        cTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAacac                                                                              >  1:2687581/1‑150 (MQ=255)

                                                                         ttgttgCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGt                                                                                           >  2:3457356/1‑136 (MQ=255)

                                                                         ttgttgCTGACCACTGCCACGGCGCCCGGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGt                                                                                           <  1:3457356/136‑1 (MQ=255)

                                                                          tgttgCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACgg                                                                            <  1:1408894/150‑1 (MQ=255)

                                                                           gttgCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGgc                                                                           <  1:599037/150‑1 (MQ=255)

                                                                           gttgCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGgc                                                                           >  2:4696496/1‑150 (MQ=255)

                                                                           gttgCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGgc                                                                           >  1:3623538/1‑150 (MQ=255)

                                                                              gCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGcgcg                                                                        >  2:4569357/1‑150 (MQ=255)

                                                                                tGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGAc                                                                      >  2:1884954/1‑150 (MQ=255)

                                                                                  aCCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGAcgc                                                                    >  1:1704258/1‑150 (MQ=255)

                                                                                    cACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGTGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCgg                                                                  >  2:3027694/1‑150 (MQ=255)

                                                                                     aCTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCggg                                                                 >  1:451616/1‑150 (MQ=255)

                                                                                              gcgcCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATg                                                        >  1:60718/1‑150 (MQ=255)

                                                                                               cgcCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGa                                                       <  1:5428714/150‑1 (MQ=255)

                                                                                                gcCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGaa                                                      <  1:3737236/150‑1 (MQ=255)

                                                                                                gcCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGaa                                                      >  1:3026178/1‑150 (MQ=255)

                                                                                                gcCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGaa                                                      <  1:3127811/150‑1 (MQ=255)

                                                                                                gcCCTGCAAACGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGaa                                                      <  2:2069826/150‑1 (MQ=255)

                                                                                                     gCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCCAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGAtct                                                 >  2:4480570/1‑150 (MQ=255)

                                                                                                      cAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGAtctc                                                >  1:2330906/1‑150 (MQ=255)

                                                                                                      cAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGAtctc                                                >  1:4132980/1‑150 (MQ=255)

                                                                                                      cAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGAtctc                                                >  1:4065580/1‑150 (MQ=255)

                                                                                                       aaGCGTTCCGCGACCTGTTCCGGGCGGGTGGCTGCGAACAGTTCGAACTGGTCGAACTGCTGCTACAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTcc                                               <  2:5597395/150‑1 (MQ=255)

                                                                                                       aaGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGaa                                                      >  1:4664965/1‑143 (MQ=255)

                                                                                                          cGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGt                                            <  1:3536019/150‑1 (MQ=255)

                                                                                                           gTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTg                                           >  2:5068814/1‑150 (MQ=255)

                                                                                                            ttCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTgg                                          >  2:5048350/1‑150 (MQ=255)

                                                                                                                gcgACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGGAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGCTCGAGAAACACGGCGCGACGCGGGCTGGGCCTGAAGTTCTCCGGTGGCagg                                      >  1:2592089/1‑150 (MQ=255)

                                                                                                                gcgACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCagg                                      <  1:408057/150‑1 (MQ=255)

                                                                                                                    ccTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCagggagg                                  <  2:516921/150‑1 (MQ=255)

                                                                                                                     cTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGTATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCTGTGGCagggaggg                                 >  1:3229323/1‑150 (MQ=255)

                                                                                                                     cTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCagggaggg                                 >  1:1281657/1‑150 (MQ=255)

                                                                                                                     cTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCagggaggg                                 <  1:3829655/150‑1 (MQ=255)

                                                                                                                      tGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAgggaggga                                >  2:4763404/1‑150 (MQ=255)

                                                                                                                      tGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAgggaggga                                >  2:130488/1‑150 (MQ=255)

                                                                                                                      acagccGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAgggaggga                                >  2:1445972/5‑150 (MQ=255)

                                                                                                                         tCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATaa                             <  1:2019793/150‑1 (MQ=255)

                                                                                                                          ccGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAg                            <  1:1443274/150‑1 (MQ=255)

                                                                                                                           cgggcgggTGGCGGCTAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAgg                           <  2:1249427/150‑1 (MQ=255)

                                                                                                                           cgggcgggTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAATGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAgg                           >  1:813874/1‑150 (MQ=255)

                                                                                                                           cgggcgggTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAgg                           <  1:987528/150‑1 (MQ=255)

                                                                                                                                       ggcgAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTtgtg                   >  1:1320634/1‑146 (MQ=255)

                                                                                                                                       ggcgAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTtgtg                   <  2:1320634/146‑1 (MQ=255)

                                                                                                                                       ggcgAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCa               >  2:4251836/1‑150 (MQ=255)

                                                                                                                                         gcagcAGTTCGACCAGTTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCAtt             >  1:473470/5‑150 (MQ=255)

                                                                                                                                            gcAGTTCGACCAGTTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGc          >  1:5508519/2‑150 (MQ=255)

                                                                                                                                            aCAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGc          >  1:4177804/1‑150 (MQ=255)

                                                                                                                                             cAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCg         >  1:5069038/1‑150 (MQ=255)

                                                                                                                                              aGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCgg        <  1:4366733/150‑1 (MQ=255)

                                                                                                                                               gTTCGACCAGTTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCggg       >  1:3151479/1‑150 (MQ=255)

                                                                                                                                                ttCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGc      >  2:2697671/1‑150 (MQ=255)

                                                                                                                                                  cGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCAt    >  2:5083450/1‑150 (MQ=255)

                                                                                                                                                   gAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCATg   >  2:5535120/1‑150 (MQ=255)

                                                                                                                                                    aaCTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCATGc  <  1:192044/150‑1 (MQ=255)

                                                                                                                                                    aaCTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCATGc  >  2:2209313/1‑150 (MQ=255)

GGTGCCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCATGC  >  NC_002947/877939‑878236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`