BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	878,092	G→T	5.4%	D30E (GAC→GAA)	hprA ←	glycerate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	878,092	0	G	T	5.4%	820.6 / 22.5	241	D30E (GAC→GAA)	hprA	glycerate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (108/120); new base T (4/9); total (112/129)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 244 total aligned reads displayed.

CCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCATGCCCGCG  >  NC_002947/877943‑878241

 cGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGt                                                                                                                                                      <  1:347054/150‑1 (MQ=255)

  ggTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTc                                                                                                                                                     <  2:5111784/150‑1 (MQ=255)

   gTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCg                                                                                                                                                    <  1:15228/150‑1 (MQ=255)

     ggcggcCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGACCAGTTCGaa                                                                                                                                                  <  2:1673658/150‑1 (MQ=255)

     ggcggcCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCGGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGACCAGTTCGAACTGTTCgcc                                                                                                                                                  <  2:2145453/150‑3 (MQ=255)

      gcggcCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAAc                                                                                                                                                 <  2:2610320/150‑1 (MQ=255)

      gcggcCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAAc                                                                                                                                                 <  2:1212194/150‑1 (MQ=255)

        ggcCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAActg                                                                                                                                               <  1:5577042/150‑1 (MQ=255)

          ccaccaGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGACCAGTTCGAActgtt                                                                                                                                             <  2:1040420/150‑3 (MQ=255)

           caccaGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGACCAGTTCGAActgttc                                                                                                                                            <  1:4538266/150‑4 (MQ=255)

           caccaGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGACCAGTTCGAActgttc                                                                                                                                            <  1:1822028/150‑4 (MQ=255)

              caGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCtt                                                                                                                                         >  1:1649430/1‑150 (MQ=255)

              caGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCtt                                                                                                                                         >  2:4988160/1‑150 (MQ=255)

               aGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTc                                                                                                                                        <  2:3585238/150‑1 (MQ=255)

                 gATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAg                                                                                                                                      <  2:4472034/150‑1 (MQ=255)

                  aTCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAgg                                                                                                                                     <  2:3122134/150‑1 (MQ=255)

                       cTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGa                                                                                                                                >  1:223419/1‑150 (MQ=255)

                       cTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGa                                                                                                                                >  2:3002708/1‑150 (MQ=255)

                       cTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGa                                                                                                                                >  2:3002576/1‑150 (MQ=255)

                             ttGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtc                                                                                                                          <  1:3048183/150‑1 (MQ=255)

                              tGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtcg                                                                                                                         <  2:3342054/150‑1 (MQ=255)

                               gCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCACGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtcga                                                                                                                        <  2:3331880/150‑1 (MQ=255)

                               gCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtcga                                                                                                                        <  2:5375405/150‑1 (MQ=255)

                                cGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtcgag                                                                                                                       >  2:5480075/1‑150 (MQ=255)

                                 gggTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtcgagg                                                                                                                      >  1:5310019/1‑150 (MQ=255)

                                     tGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGc                                                                                                                  >  2:4313984/1‑150 (MQ=255)

                                       gcggcCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGt                                                                                                                                                      <  1:4305743/112‑1 (MQ=255)

                                         ggcCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCTCTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAg                                                                                                              <  2:2701809/150‑1 (MQ=255)

                                                    cggcgTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGAtt                                                                                                   <  2:2447118/150‑1 (MQ=255)

                                                     ggcgTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTg                                                                                                  <  2:3195023/150‑1 (MQ=255)

                                                      gcgTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTgg                                                                                                 <  1:105428/150‑1 (MQ=255)

                                                      gcgTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTgg                                                                                                 <  1:343569/150‑1 (MQ=255)

                                                       cgTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCACCAAGGTCCAGGGATTGGt                                                                                                <  1:870962/150‑1 (MQ=255)

                                                       cgTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAATTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGt                                                                                                <  1:5156355/150‑1 (MQ=255)

                                                         tCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGa                                                                                              <  2:4079262/150‑1 (MQ=255)

                                                          cGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGAt                                                                                             <  2:4178915/150‑1 (MQ=255)

                                                          cGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGAt                                                                                             <  2:1326333/150‑1 (MQ=255)

                                                          cGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGAt                                                                                             <  2:2970195/150‑1 (MQ=255)

                                                           gAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATc                                                                                            <  1:102229/150‑1 (MQ=255)

                                                           gAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATc                                                                                            <  1:2832810/150‑1 (MQ=255)

                                                               aTGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCgaga                                                                                        <  2:765316/150‑1 (MQ=255)

                                                                tGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGaa                                                                                       <  1:1163993/150‑1 (MQ=255)

                                                                 gACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGTGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggtc                                                                                                                          <  1:3919398/114‑1 (MQ=255)

                                                                 gACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAaggta                                                                                                                          >  2:3919398/1‑113 (MQ=255)

                                                                   ccTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAaca                                                                                    >  2:1973472/1‑150 (MQ=255)

                                                                     ttgttgCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGt                                                                                                >  2:3457356/1‑136 (MQ=255)

                                                                      tgttgCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACgg                                                                                 <  1:1408894/150‑1 (MQ=255)

                                                                          gCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGcgcg                                                                             >  2:4569357/1‑150 (MQ=255)

                                                                              aCCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGAcgc                                                                         <  1:923841/150‑1 (MQ=255)

                                                                              aCCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGAcgc                                                                         >  1:1704258/1‑150 (MQ=255)

                                                                                cACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGTGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCgg                                                                       >  2:3027694/1‑150 (MQ=255)

                                                                                 aCTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCggg                                                                      >  1:451616/1‑150 (MQ=255)

                                                                                          gcgcCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATg                                                             >  1:60718/1‑150 (MQ=255)

                                                                                            gcCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGaa                                                           <  1:3737236/150‑1 (MQ=255)

                                                                                            gcCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGaa                                                           >  1:3026178/1‑150 (MQ=255)

                                                                                            gcCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGaa                                                           <  1:3127811/150‑1 (MQ=255)

                                                                                            gcCCTGCAAACGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGaa                                                           <  2:4671701/150‑1 (MQ=255)

                                                                                                  cAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGAtctc                                                     >  1:2330906/1‑150 (MQ=255)

                                                                                                  cAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGAtctc                                                     >  1:4132980/1‑150 (MQ=255)

                                                                                                  cAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGAtctc                                                     >  1:4065580/1‑150 (MQ=255)

                                                                                                   aaGCGTTCCGCGACCTGTTCCGGGCGGGTGGCTGCGAACAGTTCGAACTGGTCGAACTGCTGCTACAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTcc                                                    <  2:5597395/150‑1 (MQ=255)

                                                                                                   aaGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGaa                                                           >  1:4664965/1‑143 (MQ=255)

                                                                                                   aaGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTcc                                                    >  1:3656199/1‑150 (MQ=255)

                                                                                                      cGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGt                                                 <  1:3536019/150‑1 (MQ=255)

                                                                                                       gTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTg                                                >  2:5068814/1‑150 (MQ=255)

                                                                                                        ttCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTgg                                               <  2:2245322/150‑1 (MQ=255)

                                                                                                         tCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAggg                                                                                                                                    <  1:5175634/64‑1 (MQ=255)

                                                                                                         tCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGc                                              <  1:4569357/150‑1 (MQ=255)

                                                                                                            gcgACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGGAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGCTCGAGAAACACGGCGCGACGCGGGCTGGGCCTGAAGTTCTCCGGTGGCagg                                           >  1:2592089/1‑150 (MQ=255)

                                                                                                            gcgACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCagg                                           <  2:3993850/150‑1 (MQ=255)

                                                                                                            gcgACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCagg                                           <  2:1455047/150‑1 (MQ=255)

                                                                                                                 cTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGTATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCTGTGGCagggaggg                                      >  1:3229323/1‑150 (MQ=255)

                                                                                                                 cTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCagggaggg                                      >  1:1281657/1‑150 (MQ=255)

                                                                                                                 cTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCagggaggg                                      <  1:3829655/150‑1 (MQ=255)

                                                                                                                  tGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAgggaggga                                     >  2:4763404/1‑150 (MQ=255)

                                                                                                                   gTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGAt                                    >  1:4993257/1‑150 (MQ=255)

                                                                                                                     tCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATaa                                  <  1:2019793/150‑1 (MQ=255)

                                                                                                                     tCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATaa                                  >  2:1443498/1‑150 (MQ=255)

                                                                                                                      ccGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAg                                 <  1:1443274/150‑1 (MQ=255)

                                                                                                                       cgggcgggTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAgg                                >  2:1319763/1‑150 (MQ=255)

                                                                                                                            gggTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGtt                           <  2:1649430/150‑1 (MQ=255)

                                                                                                                              gTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTtgt                         <  2:1263084/150‑1 (MQ=255)

                                                                                                                               tGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTtgtg                        >  1:4259280/1‑150 (MQ=255)

                                                                                                                               tGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTtgtg                        >  2:3650106/1‑150 (MQ=255)

                                                                                                                                ggcggcGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTgg                       <  2:190650/150‑1 (MQ=255)

                                                                                                                                  cggcgAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGcc                     >  2:4303664/1‑150 (MQ=255)

                                                                                                                                  cggcgAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGcc                     >  2:3824230/1‑150 (MQ=255)

                                                                                                                                   ggcgAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTtgtg                        >  1:1320634/1‑146 (MQ=255)

                                                                                                                                     gcagcAGTTCGACCAGTTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCAtt                  >  1:473470/5‑150 (MQ=255)

                                                                                                                                        gcAGTTCGACCAGTTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGc               >  1:5508519/2‑150 (MQ=255)

                                                                                                                                        aCAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGc               >  2:1858383/1‑150 (MQ=255)

                                                                                                                                        aCAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGc               <  1:705011/150‑1 (MQ=255)

                                                                                                                                          aGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCgg             <  1:4366733/150‑1 (MQ=255)

                                                                                                                                           gTTCGACCAGTTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCggg            >  1:3151479/1‑150 (MQ=255)

                                                                                                                                            ttCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGc           >  2:2697671/1‑150 (MQ=255)

                                                                                                                                              cGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCAt         >  2:5083450/1‑150 (MQ=255)

                                                                                                                                              cGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCAt         <  1:409719/150‑1 (MQ=255)

                                                                                                                                               gAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCATg        >  2:5535120/1‑150 (MQ=255)

                                                                                                                                                aaCTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCATGc       <  1:192044/150‑1 (MQ=255)

                                                                                                                                                   tGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCATGCCcg    >  2:5251191/1‑150 (MQ=255)

CCGGTGGCGGCCACCAGGATCAGCTTCAGTTGCGGGTTGGCGGCCAGGGTTGCGGCGTCGAGCATGACCTTGTTGCTGACCACTGCCACGGCGCCCTGCAAGCGTTCCGCGACCTGTTCCGGGCGGGTGGCGGCGAACAGTTCGAACTGGTCGAACTGCTGCTTCAGGGGCGAAAGGTCGAGGTCGCCAAGGTCCAGGGATTGGTGATCGAGAAACACGGCGCGACGCGGGCTGGGCATGAAGATCTCCGGTGGCAGGGAGGGATAAGGGCGTTGTGGCCATTTGCGGGCATGCCCGCG  >  NC_002947/877943‑878241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`