Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	883,866	A→C	20.2%	intergenic (+101/‑227)	PP_0766 → / → PP_0767	hypothetical protein/LuxR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	883,866	0	A	C	20.2%	172.7 / 5.4	77	intergenic (+101/‑227)	PP_0766/PP_0767	hypothetical protein/LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (38/20);  new base C (5/12);  total (43/34)
Fisher's exact test for biased strand distribution p-value = 1.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGCCCATCAT  >  NC_002947/883717‑884015                                                                                                                                                      |                                                                                                                                                      cAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACa                                                                                                                                                       <  2:4883136/150‑1 (MQ=255) cAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACa                                                                                                                                                       <  2:615102/150‑1 (MQ=255) cAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACa                                                                                                                                                       <  1:924121/150‑1 (MQ=255) cAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACa                                                                                                                                                       <  2:4082341/150‑1 (MQ=255) cAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACa                                                                                                                                                       <  2:3855234/150‑1 (MQ=255) cAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGGGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCCATTTCCGGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACa                                                                                                                                                       <  1:942277/150‑1 (MQ=255)          tttCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCcgcg                                                                                                                                              <  2:2578397/150‑1 (MQ=255)          tttCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCACCGCCCGGTGCcgcg                                                                                                                                              >  2:4289401/1‑150 (MQ=255)           ttCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCgg                                                                                                                                             <  1:740337/150‑1 (MQ=255)            tCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGt                                                                                                                                            <  2:1462816/150‑1 (MQ=255)              cAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAg                                                                                                                                          <  1:993141/150‑1 (MQ=255)               aGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGGGGACCCGCGCCGACCGGGGCCGCGGGAgg                                                                                                                                         >  2:369577/1‑150 (MQ=255)                gCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGa                                                                                                                                        >  2:3190704/1‑150 (MQ=255)                  ccGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGc                                                                                                                                      <  2:1838098/150‑1 (MQ=255)                    gCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACCGGTTCCGCGGTAGGAGCgg                                                                                                                                    <  2:4405082/150‑1 (MQ=255)                    gCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCcgcg                                                                                                                                              >  2:4737074/1‑140 (MQ=255)                    gCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCcgcg                                                                                                                                              <  1:4737074/140‑1 (MQ=255)                    gCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCgg                                                                                                                                    <  2:4546765/150‑1 (MQ=255)                    gCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCgg                                                                                                                                    <  2:4164825/150‑1 (MQ=255)                    gCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCCCTCCAACACGTTCCGCGGGAGGAGCgg                                                                                                                                    >  2:3135422/1‑150 (MQ=255)                     cAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCggg                                                                                                                                   <  2:3878957/150‑1 (MQ=255)                      aGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGt                                                                                                                                  <  2:761792/150‑1 (MQ=255)                       gcgcTGCGTCAGTCGGGTATTCGCTAAGCTGACATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTAGTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGtt                                                                                                                                 <  2:5051919/150‑1 (MQ=255)                       gcgcTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTAGTTCCTGACCCGGCCGCTTCGCGGGTGAACCCGCGCCGCCCGGGTCCGCGGGAGGAGCGGGtg                                                                                                                                 >  2:1374678/1‑149 (MQ=255)                        cgcTGCGTCCGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCTCTCCTACCGGTTCCGCTGTAGGAGCGGGTTc                                                                                                                                <  2:4727663/150‑1 (MQ=255)                         gcTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGGACCCCCGCCAACAGGGTGCGCGGGGGGAGCGGGGTCa                                                                                                                               >  1:4135406/1‑150 (MQ=255)                            gCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCACCTAAAGGTTGCGCGGGAGGGGCGGGGTGAccc                                                                                                                            >  1:4211480/1‑150 (MQ=255)                                 aGTCGGGTATTCGATAAGCTTAAATCAGTGAACGCCGTGTGATCCCCACAACGCCCGGGCTCGCGGGGGGGTTTATATTTCCTGCCCCGGCCTCTTCGCGGGTGAACCCGCTCCTACCGGTTCCGCGGTTGGAGCGGTTTCACCCGCGaa                                                                                                                       <  1:2216047/150‑1 (MQ=37)                                   tCGGGTCTTCGCTAAGCTGAAATCACTGAACGCCGTGTGACCCCCACCACGCCCCGGCTCGCCGGGGCGTTTATATTGCCGCACCCGGCCTCTGCGCGGGTGAACCCGCGCCTACCGGTTCCGGGGTTGTAGGGGGTACACCCGCGAaga                                                                                                                     <  2:3365696/150‑1 (MQ=255)                                   tCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACCGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAaga                                                                                                                     <  2:5627599/150‑1 (MQ=255)                                   gcGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCATAACGCCCCGGCTCGCCGGGGCGTTTATATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACCGGTCCCGCGGTAGTAGCGGTTTCACCCGCGAaga                                                                                                                     <  2:4852238/149‑1 (MQ=38)                                     gggTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGTCCGCGGTATGAGCGGGTTCACCCGCGAAgggg                                                                                                                   >  2:4148103/1‑150 (MQ=255)                                                      aacccccTGACCGCCGTGTGAGCCCCCGACCGCCCCGGCGCGCGGGGGCGTTTCTTTTGCCGGCCCGGGCCTCTGCGGGGGTGACCCCGCCCCTCCGGGTTCGGCGGTTGGCGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCaa                                                                                                  <  2:1981880/144‑1 (MQ=255)                                                                  gCCGTGTGATCCCCAGACCGCCCCGGCTGGCCGGGGCGTTTCTTTTTCCTGACCCGGCCTCTTCGGGGTTGAACCCGCTCCTACCGGTTCCGCGGTAGGAGGGGGTACACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATAc                                                                                      <  2:4313858/150‑1 (MQ=38)                                                                  gCCGTGTGAGCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATGTCCTGACCCGGCCTCTGCGCGGGTGAACCCGCGCCTACCGGTTCCGCGGTAGGAGGGGTTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATAc                                                                                      <  2:4313949/150‑1 (MQ=38)                                                                       gtgtTCCCCAGACCGCCCCGGGTCGCGGTGGCTTTTCTATTTCCTGCCCGGGCCTGTGCGTGGGTGTACCGGATCCTAACGTTTCCGCGTTTGTTGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTGCTGAATACCtctc                                                                                 <  2:311845/150‑1 (MQ=255)                                                                                gaaaCGCCCCGGCGAGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGa                                                                                                                                        >  2:3944607/3‑86 (MQ=255)                                                                                gaaaCGCCCCGGCGAGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGa                                                                                                                                        <  1:3944607/84‑1 (MQ=255)                                                                                  aaCGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGGGGACCCGCTCCGACCGGTTCCGCGGGAGGGGCGGGTGCACCCGCGGAGCGGCCGGGCAAGGGGAGCAACCTCCGGGATACCACTCCCAAccccccc                                                                      >  2:3120384/1‑148 (MQ=255)                                                                                      ccccGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCGTCGCGGGTGAACCCGCACCGACAGGTTCCGCGGTGGGAGCGGGTTCACCCCCGAACAGGCCGGGCAAGGCACTCAACCGCCAGAAAACCGCACCAAACCCCCGCCCCa                                                                  >  2:405260/1‑150 (MQ=255)                                                                                       cccGGCGAGCCGGGGCG‑TTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCgg                                                                                                                                    >  1:5123376/1‑82 (MQ=25)                                                                                       cccGGCGAGCCGGGGCG‑TTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCgg                                                                                                                                    <  2:5123376/82‑1 (MQ=25)                                                                                         cGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGTACCCCCTCCAAAAGGTGACGCGGTAGGCGCGGGGTGACCCGCCCAGAGGCCCGGCAAGGGAAGCAACCAACAGAAGACCTCAGCAAAACCCCCCCCCAccc                                                               >  1:4660135/1‑150 (MQ=255)                                                                                                        tttCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCa                                                <  2:4150029/150‑1 (MQ=255)                                                                                                              gttCCTGCCCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGc                                          <  2:3152194/149‑1 (MQ=255)                                                                                                               tgccTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACCGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCt                                         <  2:4463421/148‑1 (MQ=255)                                                                                                                      cgggcccTGTTCGGGGTTTAACCCTGTCCTCCTGGTTGGGCGGTTGGCGGGTTTTAACCGGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAACCCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTc                                  <  1:3135422/145‑1 (MQ=255)                                                                                                                       ccGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCGCCTGAATACCGCTCCAAACCCCCGCCCCAGCCCCGGTCCATCAGCCATGGTGCTTTTTGTCg                                 >  2:1761176/1‑150 (MQ=255)                                                                                                                            ctctGCGCGGGTGAACCCGCTCCTCCCGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTc                            <  1:5105960/150‑1 (MQ=255)                                                                                                                                tCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCtttt                        <  2:4211480/150‑1 (MQ=255)                                                                                                                                  gcgGGTGAACCCGCTCCTACCGCGGAACCTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTcc                      >  2:4325745/1‑150 (MQ=25)                                                                                                                                    gggTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCt                                                            <  2:4441367/110‑1 (MQ=255)                                                                                                                                    gggTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCt                                                            >  1:4441367/1‑110 (MQ=255)                                                                                                                                    gggTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCcgc                    >  1:3770774/1‑150 (MQ=255)                                                                                                                                      gTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGc                                          <  1:1758257/126‑1 (MQ=255)                                                                                                                                      gTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGc                                          >  2:1758257/1‑126 (MQ=255)                                                                                                                                      gTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCgcgc                  >  1:3349078/1‑150 (MQ=255)                                                                                                                                         aaCCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTa               >  2:5625405/1‑150 (MQ=255)                                                                                                                                         aaCCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTa               >  2:5625258/1‑150 (MQ=255)                                                                                                                                           cccGCTCCTCCCGGTTCCGCGGTAGGCGGGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTAtt             <  1:369577/150‑1 (MQ=255)                                                                                                                                           cccGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTAtt             <  2:5029991/150‑1 (MQ=255)                                                                                                                                           cccGCTCCTACAGGTTCCGCGGTAGGAGCGGGTCCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTAtt             >  2:240061/1‑150 (MQ=255)                                                                                                                                            ccGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTg            >  2:9727/1‑150 (MQ=255)                                                                                                                                            ccGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTg            >  1:1907802/1‑150 (MQ=255)                                                                                                                                            ccGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTg            >  2:3574306/1‑150 (MQ=255)                                                                                                                                            ccGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTg            >  1:2023302/1‑150 (MQ=255)                                                                                                                                            ccGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTg            >  1:4855484/1‑150 (MQ=255)                                                                                                                                            ccGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTg            >  2:1399159/1‑150 (MQ=255)                                                                                                                                            ccGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTg            >  2:1399119/1‑150 (MQ=255)                                                                                                                                            ccGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTg            >  2:4237896/1‑150 (MQ=255)                                                                                                                                              gCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAAcctcct                                                                                            >  1:5244122/1‑68 (MQ=255)                                                                                                                                              gCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAAcctcct                                                                                            <  2:5244122/68‑1 (MQ=255)                                                                                                                                              gCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTgcg          >  2:3443526/1‑150 (MQ=255)                                                                                                                                                tCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGcc        >  1:2147378/1‑150 (MQ=255)                                                                                                                                                tCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGcc        >  2:3496816/1‑150 (MQ=255)                                                                                                                                                 ccTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGccc       >  1:4163397/1‑150 (MQ=255)                                                                                                                                                  cTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGCCca      >  2:2615665/1‑150 (MQ=255)                                                                                                                                                  cTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGCCca      >  1:2537670/1‑150 (MQ=255)                                                                                                                                                   tACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGCCcat     >  1:77506/1‑150 (MQ=255)                                                                                                                                                   tACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGCCcat     >  2:1272346/1‑150 (MQ=255)                                                                                                                                                   tACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGCCcat     >  1:4528843/1‑150 (MQ=255)                                                                                                                                                   tACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGCCcat     >  1:3054602/1‑150 (MQ=255)                                                                                                                                                    aCAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGccccgc    >  2:996178/1‑147 (MQ=255)                                                                                                                                                      aGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGCCcatcat  >  2:1206523/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                      CAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAGCCCCTGTCCATCAGCCATGTTGCTTTTTGTCGCAGTCTTTTCCGCGCCTATTGCGCCCATCAT  >  NC_002947/883717‑884015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑