Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 955,155 T→A 23.8% intergenic (+106/‑207) cyoE → / → alaC protoheme IX farnesyltransferase/aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947955,1550TA23.8% 207.6 / 74.8 122intergenic (+106/‑207)cyoE/alaCprotoheme IX farnesyltransferase/aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (40/51);  new base A (15/14);  total (57/65)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-01

Only 100 of 123 total aligned reads displayed.

AGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAGGCCG  >  NC_002947/955008‑955304
                                                                                                                                                     |                                                                                                                                                      
aGCCAGACCGCTGCGGATGTGCTGATGACCTTCGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTc                                                                                                                                                      <  2:5178167/150‑1 (MQ=255)
aGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTc                                                                                                                                                      <  1:4536046/150‑1 (MQ=255)
aGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTc                                                                                                                                                      <  1:4883569/150‑1 (MQ=255)
aGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTc                                                                                                                                                      <  2:2642360/150‑1 (MQ=255)
 gCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTc                                                                                                                                                      <  1:307008/150‑1 (MQ=255)
 gCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTcc                                                                                                                                                     <  2:1607750/150‑1 (MQ=255)
  ccAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCt                                                                                                                                                    <  1:3073119/150‑1 (MQ=255)
  ccAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCt                                                                                                                                                    <  2:1606734/150‑1 (MQ=255)
  ccAGACCGCTGCGGATGTGCTGATGACCTACACACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCGACAAGGTTccg                                                                                                                                                    >  2:5504990/1‑149 (MQ=255)
   cAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTg                                                                                                                                                   <  2:4465677/150‑1 (MQ=255)
    aGACCGCTGCGGATGTGGTGATGACCTACTCACGCTTAAACTGCTGCCTGTTTCTCGAAAACGCCGGCCACTGTGCCGTGG‑TTTTTTTTTGGTGGTATTTTTCCTGTGCCTGCCTCTTCGCGGGTAAA‑CCCGCTCATACAAGTTTCCTGt                                                                                                                                                  <  2:5026714/150‑1 (MQ=255)
     gACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTa                                                                                                                                                 <  1:2058413/150‑1 (MQ=255)
     gACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTa                                                                                                                                                 <  2:3844266/150‑1 (MQ=255)
      aCCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAg                                                                                                                                                <  2:4577350/150‑1 (MQ=255)
      aCCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑‑TTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAgg                                                                                                                                               <  1:4464718/150‑1 (MQ=255)
         gCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TATTATTTTGGGGGTATTTTT‑CTGTGCTGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAGGAACCTTGTAGGAga                                                                                                                                            <  2:238713/150‑2 (MQ=25)
          cTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAGGAACCTTGTAGGAGc                                                                                                                                            <  1:2730839/150‑1 (MQ=38)
          cTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGc                                                                                                                                            >  2:4643412/1‑150 (MQ=255)
          cTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGc                                                                                                                                            <  1:3494738/150‑1 (MQ=255)
          cTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCGGTAGGAGc                                                                                                                                            <  2:1678479/150‑1 (MQ=255)
           tGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCGCCAACCAGGTTGCGGTAGGGGCg                                                                                                                                           >  2:1939530/1‑150 (MQ=255)
           tGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGGAAA‑CCCGCGCCTACAAGGTTGCGGTAGGAGCg                                                                                                                                           >  2:2049229/1‑150 (MQ=255)
           tGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTT‑CTGTGCTGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAGGAACCTTGTAGGAGCgg                                                                                                                                          <  2:1711058/150‑1 (MQ=25)
            gCGGATGTGCTGATGACCTACGCACGCTGACACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TATTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACCAGGTTCCTGTAGGAGCgg                                                                                                                                          <  1:2609220/150‑1 (MQ=255)
            gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAGGAACCTTGTAGGAGCgg                                                                                                                                          <  1:963216/150‑1 (MQ=38)
            gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAGGAACCTTGTAGGAGCgg                                                                                                                                          <  2:832771/150‑1 (MQ=38)
            gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAGGAACCTTGTAGGAGCgg                                                                                                                                          <  2:2811046/150‑1 (MQ=38)
            gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCgg                                                                                                                                          <  2:4841917/150‑1 (MQ=255)
            gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCgg                                                                                                                                          <  2:3871242/150‑1 (MQ=255)
            gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTT‑CTGTGCTGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAGGAACCTTGTAGGAGCggg                                                                                                                                         <  1:929228/150‑1 (MQ=25)
            gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTCCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCgg                                                                                                                                          <  2:1830476/150‑1 (MQ=255)
            gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCGGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAGGAACCTTGTAGGAGCgg                                                                                                                                          <  2:2856621/150‑1 (MQ=37)
             cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCggg                                                                                                                                         <  1:4772386/150‑1 (MQ=255)
             cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCggg                                                                                                                                         <  2:2509922/150‑1 (MQ=255)
             cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCggg                                                                                                                                         <  1:324404/150‑1 (MQ=255)
             cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCGTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCggg                                                                                                                                         <  1:2695266/150‑1 (MQ=255)
               gATGTACTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCCCGCCGACCAGGTTCCGGGAGGAGCGGGtt                                                                                                                                       >  2:1913899/1‑150 (MQ=255)
                 tgtgCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTa                                                                                                                                     <  1:2861260/150‑1 (MQ=255)
                        atgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTGGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGGAAA‑CCCGCTCCGCCCAGGGTCCGGTAGGAGCGGGTTTGCCcgcgc                                                                                                                              >  2:303870/1‑149 (MQ=255)
                          gaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCAACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAag                                                                                                                            >  1:5556210/1‑150 (MQ=255)
                             cTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑ACCGCACCCACAGGGACCGGGTTGGCGCGGGTTTAACCCCCAAgggg                                                                                                                         >  1:5062700/1‑150 (MQ=255)
                                cgcacgcTGAAACTGCTGCCTGTGTCACGAAACCCCCGGCCCCTGGGCCGGGG‑TTTTTTTTTGGGGGTATTTTGCCTGGGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGccg                                                                                                                      <  2:5062700/150‑2 (MQ=21)
                                          acctgctgCGTGTGTCACGAAACCCCCGGCCACTGTGCCGGGG‑TTTTTTTTGGGGGGTATTTTTCCTGTGCCGGCCTCTGCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑Gaaaa                                                                                                           <  2:4357458/148‑1 (MQ=37)
                                           aCTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTT‑CTGTGCTGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACA‑Ggaaaaa                                                                                                         <  2:5557505/150‑2 (MQ=17)
                                                  ccTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCGCCGACAAGGTTGCGGTAGGAGCGGGGTTACCCGCGCAGAGGCCAGCACA‑Gaaaaataaaaaa                                                                                                   >  2:4731305/1‑149 (MQ=38)
                                                  ccTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTGTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCGCCGCCCATGGTCCGGTCGGGGCGGGGTTTGCCGCGCATGGTCCAGCCCA‑Gacaaattaaact                                                                                                   >  2:4731156/1‑148 (MQ=255)
                                                            gAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCGGTTGGGGCGGGTTTTCCCGCGAAGGGGCCCGCACA‑GTAAAATAAAAATACCTGAtttt                                                                                         >  2:3063388/1‑150 (MQ=38)
                                                              aaaCCCCGGCCCCTGTGCCGGGT‑TTTTTTTTGGGGGGTATTTTTCCGGGGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACACGGTTCCTGTCGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCa                                                                                       <  1:4192554/150‑1 (MQ=37)
                                                                 ccccGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCCCGAAGAGGCCAGCACA‑GAAAAATAAAAATAACAGATTTTAaaaa                                                                                    >  2:5273588/1‑150 (MQ=255)
                                                                  cccGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCCCACCAAAAAGGTTGCGGGAGGAGCGGGTTTACACGCGAACAGGGCAGCACA‑GAAAAAAAAAAAAAACAGATTTTGaaaaa                                                                                   >  2:2044172/1‑150 (MQ=255)
                                                                  cccGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑ACCGCTCCTACAAGGTTCCGGTAGGAGCCGGGTTACCCGCGGAGAGGCCAGCCCC‑GAAAAATAAAAAAACCTGATTTTCaaaaa                                                                                   >  2:2044083/1‑150 (MQ=38)
                                                                  cccGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTAGCGGGTAAA‑ACAGCGCCAACAAGGTTCCTGTAGGTGCGGGGTTACACGCGAGTAGGCCAGCACA‑CAAAAATAAAAAAAACGGATTTTaaaaaa                                                                                   >  2:1424603/1‑150 (MQ=255)
                                                                    cGGCCCGTGGGCCGGGT‑TTTTTTTTTGGGGTTATTTTTCCTGGGCCGGCCTCTCCGCGGGTAAA‑CCCGCTCCTACATGGTTCCTTTTGGAGGGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAAt                                                                                 <  2:4696876/150‑1 (MQ=25)
                                                                    cGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑ACCGCTCCTACAAGGTTACGGGGGGTGCGGGTTTTCCCGCGTAGAGGGCAGCACA‑GAAAAATAAAAATAACTGATTTTGaaaaaaa                                                                                 >  2:1944787/1‑149 (MQ=37)
                                                                     ggCCACTGGGCGGGGG‑TTTTTTTGTGGGGTTATTTTTCCTGTGCCGCCCTCTTCGCGGGTAAC‑CCCGCTCCTCCACTGTTCCTGTAGGAGGGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAACAAATa                                                                                <  2:247695/150‑1 (MQ=25)
                                                                          ctggcccGGGG‑ATTTTTTTGGGGGGTATTTTTCCGGGGCCGGCCCGTGCG‑GGGTAAACCCCGCCCCCACCCGGTGCCTGCGGGCGGGGGTTAACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCt                                                                           <  1:741407/145‑1 (MQ=255)
                                                                                   gg‑TTTTTTTTTGGGGGTATTTTTCCGGTGCCGGCGTCTTCGCGGGTAAA‑CCCGCTCCTCCACGGTTCGTGTAGGAGCGGGTTTCCCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTc                                                                  <  2:281057/150‑1 (MQ=38)
                                                                                    gttttttttttGGGGTTATTTTCCCTGGGCGGGCCCGTGGGCGGTAAAA‑CCCGCACCAACAGGTTGCCTGTCGGCGCGGTTTAACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTc                                                                  <  1:2030073/148‑1 (MQ=255)
                                                                                       tttttttgGGGTTTATTTTCCCTGGGCGGGCCTCTCCGCGGGAAAC‑CCCCCCCCAACACGTTCCCTGTCGGTGGGGTTTAACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCtaa                                                               <  1:1391381/150‑1 (MQ=255)
                                                                                              tGGGGTTATTCTGCCTGTGCCGCCCACTTCGGGGGAAAC‑CCCGCCCCCACAAGTTTCCTTATGGTGGGGTTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACa                                                        <  1:5361724/150‑1 (MQ=255)
                                                                                                        tttCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATc                                              <  2:3517668/150‑1 (MQ=255)
                                                                                                               tgCCGGCCTCTTCGGGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCGGTTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACa                                       <  2:1037927/150‑1 (MQ=255)
                                                                                                               ggCCGGCCTCTGCGGGGGTAAA‑CCCGCTCCTACCCGGTTCCTGGAGGAGGGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACa                                       <  2:512062/149‑1 (MQ=38)
                                                                                                                         ttCGCGGGTAAA‑ACCGCTCCTACAAGGTTCCGGGAGGAGAGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTAAAAAAATAGATCAGAAAAGTTCTAATAAAACAGGAAATTAACCTTTAAAGGCATCCTGt                             >  2:1685743/1‑150 (MQ=38)
                                                                                                                            gcgGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCCTCCTGTTTc                          >  2:4653611/1‑150 (MQ=255)
                                                                                                                              gggTACA‑CCCGCTCCTCCACGGTCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCgg                        <  1:5504990/150‑1 (MQ=255)
                                                                                                                               gtcaaaccccGCTCCAAACCGGTGCGTGTTGGCGGGGTTTCACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCgg                        <  1:1903268/143‑1 (MQ=255)
                                                                                                                                gTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCa                      >  1:837404/1‑150 (MQ=255)
                                                                                                                                 tAAA‑CCCGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCAc                     >  2:652168/1‑150 (MQ=37)
                                                                                                                                    a‑CCCGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAt                  >  1:4363236/1‑150 (MQ=37)
                                                                                                                                    a‑CCCGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAt                  >  1:495000/1‑150 (MQ=37)
                                                                                                                                      cccGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc                 >  2:885187/1‑150 (MQ=38)
                                                                                                                                      cccGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc                 >  1:2065325/1‑150 (MQ=38)
                                                                                                                                      cccGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc                 >  1:3193356/1‑150 (MQ=38)
                                                                                                                                      cccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc                 >  2:68563/1‑150 (MQ=255)
                                                                                                                                      cccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc                 <  2:5556210/150‑1 (MQ=255)
                                                                                                                                       ccGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGAAAAATACAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc                 >  1:4948865/1‑150 (MQ=25)
                                                                                                                                       ccGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGAt                                                                             <  2:549604/89‑1 (MQ=18)
                                                                                                                                       ccGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGAt                                                                             >  1:549604/1‑89 (MQ=18)
                                                                                                                                       ccGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATGCTTTACAGGCATCCTGTTTCGGCACTAtct                >  2:4799909/1‑150 (MQ=37)
                                                                                                                                       ccGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCAGCGAAGAGGCCAGCACA‑GAAATATCAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACCGGAAATTATCCGTTACAGGCATCCTGTTTCGGCACTAtct                >  2:3526283/1‑150 (MQ=37)
                                                                                                                                       ccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAACTTATCCTTTACAGGCATCCTGTTTCGGCACTAgct                >  2:1976664/1‑150 (MQ=255)
                                                                                                                                       ccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct                >  1:5094165/1‑150 (MQ=255)
                                                                                                                                        cGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtctt               >  1:3881085/1‑150 (MQ=38)
                                                                                                                                         gCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc              >  1:383822/1‑150 (MQ=255)
                                                                                                                                         gCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc              >  1:4005166/1‑150 (MQ=255)
                                                                                                                                         gCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc              <  2:1239313/150‑1 (MQ=255)
                                                                                                                                          cTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttct             >  1:249253/1‑150 (MQ=255)
                                                                                                                                           tCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTg            >  1:2204079/1‑150 (MQ=255)
                                                                                                                                            ccTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGa           <  2:2427303/150‑1 (MQ=255)
                                                                                                                                            ccTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGa           <  2:1314005/150‑1 (MQ=255)
                                                                                                                                              tACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt         >  1:3581994/1‑150 (MQ=255)
                                                                                                                                              tACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt         >  1:2924987/1‑150 (MQ=255)
                                                                                                                                              tACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt         >  2:2413348/1‑150 (MQ=255)
                                                                                                                                              tACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt         >  1:1504781/1‑150 (MQ=255)
                                                                                                                                               aCAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTc        >  1:3310559/1‑150 (MQ=255)
                                                                                                                                               aCAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTc        >  1:1826997/1‑150 (MQ=255)
                                                                                                                                                    gTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAGgcc   >  2:113445/1‑150 (MQ=255)
                                                                                                                                                    gTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAGTCAGgcc   >  1:3073621/1‑150 (MQ=255)
                                                                                                                                                     ttCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAGgccg  >  2:3681233/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAA‑CCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACA‑GAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCAGGCCG  >  NC_002947/955008‑955304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑