Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 968,878 G→T 8.8% intergenic (‑308/+23) PP_0831 ← / ← PP_t68 hypothetical protein/tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947968,8780GT 8.8% 601.1 / 37.7 193intergenic (‑308/+23)PP_0831/PP_t68hypothetical protein/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (103/72);  new base T (10/7);  total (114/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

Only 100 of 214 total aligned reads displayed.

TAACAATACTTTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAATCGG  >  NC_002947/968729‑969027
                                                                                                                                                     |                                                                                                                                                     
tAACAATACTTTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAaccgaccg                                                                                                                                                       >  1:4776046/1‑150 (MQ=255)
   aaaTACTTGCTGAATATATCTGACTTATGACAGCTTAAGTGCTAGCGCATGGGGCGGCCAATAGACGGCAGGTTTGGGGGGAGGCGCGGGAGTCCGGGGGGCGGCGTCAACGACGTGCGTGATTCGCAGGCACAAAAAAACCGACCGTaa                                                                                                                                                    <  2:711380/149‑1 (MQ=255)
    aaTACTTTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAg                                                                                                                                                   >  1:1534252/1‑150 (MQ=255)
    aaTACTTTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAg                                                                                                                                                   >  1:5308665/1‑150 (MQ=255)
    aaTACTTTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAg                                                                                                                                                   >  2:1275024/1‑150 (MQ=255)
      tACTTTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGt                                                                                                                                                 >  1:4501192/1‑150 (MQ=255)
         tttCGGAATATCTCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATCGAATGCAGGTATGGGGGGAGGCGCGGGAGTCCGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCgg                                                                                                                                              <  2:294341/150‑1 (MQ=255)
         tttCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCgg                                                                                                                                              >  1:3741105/1‑150 (MQ=255)
         ttgcgcaaTATCTCTGACGAATGACAGCTTAAGTGCCCGCGCACGGGGCGGCCAATAGAATGCCGGTCGGGGGGGCGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCgg                                                                                                                                              <  2:1157540/144‑1 (MQ=255)
         cttCGGATTTTAGCTGACGAATGACAGCTTACGTGCTAGCGCACGGGGCGGCCACTAGAATGCCGGTAGGGGGGGAGGCGCGGGAGTCCGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCgg                                                                                                                                              <  2:425392/149‑1 (MQ=255)
          ttCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCACGGGGCGGCCAATAGAATGCTGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCTTAcggtcggt                                                                                                                                             <  2:1060653/150‑1 (MQ=255)
          ttCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCTTAcggtcggt                                                                                                                                             <  2:3681116/150‑1 (MQ=255)
          ttCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGt                                                                                                                                             >  1:3370051/1‑150 (MQ=255)
          ttCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAAGCGCAGGCACAAAAAAACCGACCCTAAGGTCGGt                                                                                                                                             >  2:3614347/1‑150 (MQ=255)
          tgcGGAATATATCGGCCGAATCACCGCGAACGTGCTAGCGCCCGGGGCGCCCGACAGAATGCCGGTCGGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGCCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCTTAcggtcggt                                                                                                                                             <  2:2448198/148‑1 (MQ=255)
            cgcaaTATACCTGACGAATCACACCTTAAGTCTAAGCCCAAGGGGCGGCCAATACCACCCACGTCTGGGGGCACCCCCGGGAGTCCGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGtta                                                                                                                                           <  2:1198451/147‑2 (MQ=255)
            cGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGttt                                                                                                                                           >  1:2329588/1‑150 (MQ=255)
            cGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGttt                                                                                                                                           >  1:2123320/1‑150 (MQ=255)
             gcaaTATATCTGACGAATGACCGCTCACGTGCTAGCGCAAGGGGCGGCCATTAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGtttt                                                                                                                                          <  2:3998963/148‑1 (MQ=255)
              gAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGGGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGttttt                                                                                                                                         >  2:1330561/1‑150 (MQ=255)
                  atatCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCg                                                                                                                                     >  1:3308689/1‑150 (MQ=255)
                  atatCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCg                                                                                                                                     >  2:69865/1‑150 (MQ=255)
                    atCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGa                                                                                                                                   >  1:256579/1‑150 (MQ=255)
                       tGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGAttt                                                                                                                                >  1:4747733/1‑150 (MQ=255)
                       tGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGAttt                                                                                                                                >  1:4317566/1‑150 (MQ=255)
                       tGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGAttt                                                                                                                                >  1:5150841/1‑150 (MQ=255)
                       tGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAAGAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGAttt                                                                                                                                >  2:3048252/1‑150 (MQ=255)
                        gACTAATGACACCTTCAGTGCTCGCGCACGGGGCGTCCATTAGAATGCAGTGCGGGGGTGAGGCCCGGTAGTCTGGGGGGCGGCGTCAACTACGTGCTTGATTCGCAGGCACAAAAACACCGACCGTAAGGTCGGTTTTTTCCGGATTTg                                                                                                                               <  2:3495721/150‑1 (MQ=255)
                        gACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTg                                                                                                                               >  2:3755156/1‑150 (MQ=255)
                         aCGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTgg                                                                                                                              >  2:4529968/1‑150 (MQ=255)
                                       aaGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGgaga                                                                                                                >  2:5232841/1‑150 (MQ=255)
                                          tGCGAGCGCAAGGGGCGCCCAACCGACTGCCGGCATGGGGGGAGGCGCGGGAGTCCGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTc                                                                                                             <  2:856994/150‑1 (MQ=255)
                                           gCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCg                                                                                                            >  1:1673168/1‑150 (MQ=255)
                                                   aaGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCa                                                                                                    >  2:5086704/1‑150 (MQ=255)
                                                              aaTAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGaa                                                                                         >  1:4452139/1‑150 (MQ=255)
                                                              aaTAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGaa                                                                                         >  2:147000/1‑150 (MQ=255)
                                                                tAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGaaaa                                                                                       >  1:481763/1‑150 (MQ=255)
                                                                 aGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGaaatc                                                                                      >  1:2512736/1‑148 (MQ=255)
                                                                 aGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAAc                                                                                      >  1:2955004/1‑150 (MQ=255)
                                                                      gCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGAtt                                                                                 <  2:4605064/150‑1 (MQ=255)
                                                                       ccggTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGAttt                                                                                <  2:4124161/148‑1 (MQ=255)
                                                                        cgggaTGGGGGGCGGCGCGGGCGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGAtttt                                                                               <  2:5350982/146‑1 (MQ=255)
                                                                        cggTATGGGGGGCGGCGCGGGAGTCCGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGAtttt                                                                               <  2:596365/149‑1 (MQ=255)
                                                                        aGGTCTGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCATCAACGAGGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGAtttt                                                                               <  2:2130724/150‑1 (MQ=255)
                                                                        aGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGAtttt                                                                               >  2:503363/1‑150 (MQ=255)
                                                                         ggTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTg                                                                              >  2:4928429/1‑150 (MQ=255)
                                                                          gTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGa                                                                             >  1:28582/1‑150 (MQ=255)
                                                                           tATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGaa                                                                            <  2:466671/150‑1 (MQ=255)
                                                                              ggggggAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTACCACTCCTTTCGAAAACGGATTTTTAATcc                                                                         >  1:3883483/1‑150 (MQ=255)
                                                                                   gAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCcgccgc                                                                    >  2:4718045/1‑150 (MQ=255)
                                                                                      gcgcGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTc                                                                 >  2:3246826/1‑150 (MQ=255)
                                                                                         cgGGAGTCAGGGGGGGGGCGTCAACGACGTGCGTGAATCGCAGGCAAAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTAc                                                              <  2:260295/150‑1 (MQ=255)
                                                                                                  ccgccgcggcgTCAACGACGTGCGTTAACCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGc                                                     <  1:4222241/145‑1 (MQ=255)
                                                                                                   gggggCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGcc                                                    >  1:2671612/1‑150 (MQ=255)
                                                                                                       gcggcgTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCa                                                <  2:4501192/150‑1 (MQ=255)
                                                                                                         ggcgTCAACGAGGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAgg                                              <  1:4864413/150‑1 (MQ=255)
                                                                                                          gcgTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAggg                                             >  1:5206382/1‑150 (MQ=255)
                                                                                                              cAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTg                                         <  2:3854272/150‑1 (MQ=255)
                                                                                                               aaCGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGTCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTgg                                        >  2:2254864/1‑150 (MQ=255)
                                                                                                                acgactTGCGTTAATCGCAGCCAAAAAAAAACCGACCGTAAGGTCGGATTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGgc                                       <  2:2530201/150‑1 (MQ=255)
                                                                                                                acgacgTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGgc                                       <  2:678522/150‑1 (MQ=255)
                                                                                                                acgacgTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGgc                                       <  1:4659563/150‑1 (MQ=255)
                                                                                                                  gacgTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGgcgc                                     >  1:817183/1‑150 (MQ=255)
                                                                                                                  gacgTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGCGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTAACAATTCCGCCATCAGGGCGTGgagc                                     >  2:5626600/1‑150 (MQ=255)
                                                                                                                     gtgcgttAAGCGCAGGAAAAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCa                                  <  1:5184388/150‑1 (MQ=255)
                                                                                                                     gtgcgtgAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCa                                  <  1:3612524/150‑1 (MQ=255)
                                                                                                                     gtgcgtgAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCa                                  <  1:4937019/150‑1 (MQ=255)
                                                                                                                     gggcggtaaTCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGGGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCa                                  <  1:698677/143‑1 (MQ=255)
                                                                                                                        cgtgAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGta                               >  1:1390759/1‑150 (MQ=255)
                                                                                                                         gtgAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGtat                              >  1:3250647/1‑150 (MQ=255)
                                                                                                                          tgAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGtata                             >  1:1554732/1‑150 (MQ=255)
                                                                                                                            aagcGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTGGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATaaa                           <  2:1436719/147‑1 (MQ=255)
                                                                                                                             aTCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAag                          <  1:368335/150‑1 (MQ=255)
                                                                                                                               cGCAGGCACAAAAAAACCGACCGTAGGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAagag                        <  1:5525679/150‑1 (MQ=255)
                                                                                                                                gCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAgg                       >  1:383697/1‑150 (MQ=255)
                                                                                                                                 cAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATATAGAGGt                      >  2:2987734/1‑150 (MQ=255)
                                                                                                                                     cacaAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCg                  >  1:2801343/1‑150 (MQ=255)
                                                                                                                                      acaAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGc                 <  2:2783781/150‑1 (MQ=255)
                                                                                                                                      acaAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGc                 <  1:3755156/150‑1 (MQ=255)
                                                                                                                                      acaAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGc                 >  2:5319394/1‑150 (MQ=255)
                                                                                                                                       caAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCt                >  2:5571387/1‑150 (MQ=255)
                                                                                                                                        aaaaaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCCGTCTAAAGAGGTGCCGCTg               >  1:3309066/1‑150 (MQ=255)
                                                                                                                                        aaaaaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTATACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTg               >  2:198934/1‑150 (MQ=255)
                                                                                                                                         aaaaaaCCGACCTTACGGTCGGTTTTTTGCGGATTTGGTGCCCCGAGGGAGACACGAACACCCACGCCATTCGAAAACGGATTTTTTAAACGCCGCGTCGACCAAATCCGCCATCAGGGCGTGGCGCGCCGTATAAAGAGGTGCCGCTgg              >  1:3967960/1‑150 (MQ=255)
                                                                                                                                         aaaaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTgg              <  1:3048366/150‑1 (MQ=255)
                                                                                                                                           aaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAGGAGGTGCCGCTggtc            >  1:3177786/1‑150 (MQ=255)
                                                                                                                                            aaaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGgcgcgc                                   <  1:1016937/126‑1 (MQ=255)
                                                                                                                                            aaaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGgcgcgc                                   >  2:1016937/1‑126 (MQ=255)
                                                                                                                                            aaaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcg           >  2:5267198/1‑150 (MQ=255)
                                                                                                                                             aaCCGCCGGGAAGGTCGGTTTTTTCCGGATTTGGGGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAACCGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcgg          <  2:4422084/150‑1 (MQ=255)
                                                                                                                                             aaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTTCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGCATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcgg          >  2:1306147/1‑150 (MQ=255)
                                                                                                                                             aaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGCGGGAGACTCGCACTCCCACTCCTGTCGCAACCGGGGTTTGAATCCGCCGCGTGTACCCAATCCGCCCTCCGGGCGTGGCGCGCCGGGGAGAGCGGTGGCGCTgggcgg          >  1:5344906/1‑150 (MQ=255)
                                                                                                                                             aaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACACCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTACGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcgg          >  2:1567636/1‑150 (MQ=255)
                                                                                                                                             aaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcgg          >  1:575119/1‑150 (MQ=255)
                                                                                                                                             aaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcgg          <  1:1447427/150‑1 (MQ=255)
                                                                                                                                              accgaccgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcggt         <  1:69776/150‑1 (MQ=255)
                                                                                                                                              accgaccgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcggt         <  2:3308944/150‑1 (MQ=255)
                                                                                                                                               ccgacctTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATATTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcggtc        >  2:2420597/1‑150 (MQ=255)
                                                                                                                                                    cgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAATCg   >  1:896392/1‑150 (MQ=255)
                                                                                                                                                     gTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAATCgg  <  2:4776046/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAACAATACTTTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAATCGG  >  NC_002947/968729‑969027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑