Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,071,287 T→C 5.7% S89G (AGC→GGC)  PP_0924 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,071,2870TC 5.7% 792.7 / 22.4 244S89G (AGC→GGC) PP_0924hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (116/114);  new base C (4/10);  total (120/124)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 258 total aligned reads displayed.

CTCGGCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAATTCTGC  >  NC_002947/1071138‑1071434
                                                                                                                                                     |                                                                                                                                                   
cTCGGCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCTGGCTGAATTCTTCTTCGCTGTAGTAACGGCCCAGgct                                                                                                                                                     >  1:1470721/1‑150 (MQ=255)
cTCGGCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGgct                                                                                                                                                     >  1:556985/1‑150 (MQ=255)
cTCGGCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTACAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGgct                                                                                                                                                     >  2:3553803/1‑150 (MQ=255)
cTCGGCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTACAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGgct                                                                                                                                                     >  2:3258695/1‑150 (MQ=255)
 tCGGCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGgctg                                                                                                                                                    >  2:3200273/1‑150 (MQ=255)
   ggCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGgctgct                                                                                                                                                  <  1:5551298/150‑1 (MQ=255)
    gCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGctgctg                                                                                                                                                 >  2:871648/1‑150 (MQ=255)
           cGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCt                                                                                                                                          <  2:688285/150‑1 (MQ=255)
           cGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCt                                                                                                                                          <  1:3877555/150‑1 (MQ=255)
           cGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCt                                                                                                                                          <  2:3326672/150‑1 (MQ=255)
            gCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCACCAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTg                                                                                                                                         <  2:4136995/150‑1 (MQ=255)
            gCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTg                                                                                                                                         <  2:4744990/150‑1 (MQ=255)
            gCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTg                                                                                                                                         <  2:4318074/150‑1 (MQ=255)
             ccccAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCCGCCAGCAGCCTgg                                                                                                                                        <  2:948395/150‑1 (MQ=255)
              cccAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGt                                                                                                                                       >  2:2570521/1‑150 (MQ=255)
              cccAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGt                                                                                                                                       >  1:3109137/1‑150 (MQ=255)
              cccAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGt                                                                                                                                       >  2:1507786/1‑150 (MQ=255)
              cccAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGt                                                                                                                                       >  2:4491751/1‑150 (MQ=255)
                cAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGa                                                                                                                                     <  1:2255841/150‑1 (MQ=255)
                cAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCCGCCAGCAGCCTgggcc                                                                                                                                     <  1:3681818/150‑4 (MQ=255)
                      aCGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGc                                                                                                                               <  2:3828116/150‑1 (MQ=255)
                        ggCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAg                                                                                                                             >  1:5474213/1‑150 (MQ=255)
                          cGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGccgaa                                                                                                                           <  2:4090541/150‑5 (MQ=255)
                              ggTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGggt                                                                                                                       <  1:4601801/150‑1 (MQ=255)
                                tACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGggtgg                                                                                                                     >  1:5472856/1‑150 (MQ=255)
                                  ccgccgTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGgtggtg                                                                                                                   <  2:3462102/150‑1 (MQ=255)
                                  ccgccgTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTACTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGgtggtg                                                                                                                   <  2:3213616/150‑1 (MQ=255)
                                   cgccgTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGtggtgg                                                                                                                  >  2:40547/1‑150 (MQ=255)
                                   cgccgTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGtggtgg                                                                                                                  >  1:660276/1‑150 (MQ=255)
                                      cgTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCaa                                                                                                               <  1:4886529/150‑1 (MQ=255)
                                            ggTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCCGtt                                                                                                         >  2:3173179/1‑150 (MQ=255)
                                            ggTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGtt                                                                                                         >  2:1424398/1‑150 (MQ=255)
                                                gTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGcc                                                                                                     <  1:1590947/150‑1 (MQ=255)
                                                gTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGcc                                                                                                     <  2:3625079/150‑1 (MQ=255)
                                                 tCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCg                                                                                                    >  2:2363226/1‑150 (MQ=255)
                                                    ggggCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTg                                                                                                 <  2:800850/150‑1 (MQ=255)
                                                         gACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAg                                                                                            >  1:582718/1‑150 (MQ=255)
                                                         gACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAg                                                                                            <  2:171083/150‑1 (MQ=255)
                                                         gACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAg                                                                                            >  2:2265562/1‑150 (MQ=255)
                                                          aCGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAgg                                                                                           >  2:2062753/1‑150 (MQ=255)
                                                              aGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTcgc                                                                                       <  1:3495782/150‑1 (MQ=255)
                                                              aGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTcgc                                                                                       <  2:4102124/150‑1 (MQ=255)
                                                               ggTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTcgcg                                                                                      >  2:1219984/1‑150 (MQ=255)
                                                                     gCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACAcc                                                                                >  2:5499449/1‑150 (MQ=255)
                                                                        cAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAAt                                                                             >  1:2367766/1‑150 (MQ=255)
                                                                        cAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAAt                                                                             >  1:4419473/1‑150 (MQ=255)
                                                                        cAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAAt                                                                             <  2:5218099/150‑1 (MQ=255)
                                                                               aGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTggcgg                                                                      <  2:1983145/150‑1 (MQ=255)
                                                                                ggATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTggcggc                                                                     >  1:3557426/1‑150 (MQ=255)
                                                                                    cGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTg                                                                 >  1:740769/1‑150 (MQ=255)
                                                                                     gggCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGc                                                                <  2:1171543/150‑1 (MQ=255)
                                                                                      ggCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCa                                                               >  2:3323441/1‑150 (MQ=255)
                                                                                         cTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAcgt                                                            >  2:1540476/1‑150 (MQ=255)
                                                                                          ttGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAcgtc                                                           <  2:1093434/150‑1 (MQ=255)
                                                                                          ttGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAcgtc                                                           >  2:2661729/1‑150 (MQ=255)
                                                                                                  gCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAgtcgt                                                   <  1:2817829/150‑1 (MQ=255)
                                                                                                   cAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTACTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAgtcgtc                                                  <  2:4857378/150‑1 (MQ=255)
                                                                                                    aaCAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTcc                                                 <  1:961437/150‑1 (MQ=255)
                                                                                                     aCAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCa                                                <  2:1320875/150‑1 (MQ=255)
                                                                                                      cAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCAt                                               >  2:3362899/1‑150 (MQ=255)
                                                                                                         gCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTc                                            >  1:851833/1‑150 (MQ=255)
                                                                                                         gCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTc                                            >  2:4220591/1‑150 (MQ=255)
                                                                                                         gCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTc                                            <  1:4491772/150‑1 (MQ=255)
                                                                                                           cGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCa                                          >  1:4728150/1‑150 (MQ=255)
                                                                                                             tGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAgg                                        >  1:4386253/1‑150 (MQ=255)
                                                                                                                  gCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTg                                   <  1:565877/150‑1 (MQ=255)
                                                                                                                    tGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCt                                 <  1:3381492/150‑1 (MQ=255)
                                                                                                                    tGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCt                                 >  1:1026234/1‑150 (MQ=255)
                                                                                                                      aaTTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCg                               >  2:559788/1‑150 (MQ=255)
                                                                                                                        ttcttcttcGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGc                             <  1:3968791/150‑1 (MQ=255)
                                                                                                                        ttcttcttcGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGc                             <  1:1294018/150‑1 (MQ=255)
                                                                                                                        ttcttcttcGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGc                             >  1:4992579/1‑150 (MQ=255)
                                                                                                                           ttcttcGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCAcc                          >  1:3537247/1‑150 (MQ=255)
                                                                                                                           ttcttcGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCAcc                          >  1:2655500/1‑150 (MQ=255)
                                                                                                                            tcttcGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCt                         <  1:3258695/150‑1 (MQ=255)
                                                                                                                              ttcGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCttt                       <  1:1507786/150‑1 (MQ=255)
                                                                                                                              ttcGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCttt                       <  1:2261682/150‑1 (MQ=255)
                                                                                                                                cGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGa                     <  1:1079623/150‑1 (MQ=255)
                                                                                                                                 gCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAg                    >  1:306548/1‑150 (MQ=255)
                                                                                                                                  cTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGc                   >  1:1346630/1‑150 (MQ=255)
                                                                                                                                     tAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTg                >  2:3981679/1‑150 (MQ=255)
                                                                                                                                     tAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTg                <  2:585080/150‑1 (MQ=255)
                                                                                                                                       gAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGc              >  2:3426788/1‑150 (MQ=255)
                                                                                                                                         aaCGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCt            >  2:1971494/1‑150 (MQ=255)
                                                                                                                                         aaCGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCt            >  2:51434/1‑150 (MQ=255)
                                                                                                                                          gatcaccAGGCCGCCAGCAGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTGTGAGCTTGGCCtt           >  2:2919753/6‑150 (MQ=255)
                                                                                                                                          aCGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCtt           <  1:2570521/150‑1 (MQ=255)
                                                                                                                                          aCGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCtt           <  1:4275636/150‑1 (MQ=255)
                                                                                                                                          aCGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCtt           <  2:3109137/150‑1 (MQ=255)
                                                                                                                                            ggCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGa         <  2:4504196/150‑1 (MQ=255)
                                                                                                                                             gCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGaa        >  1:1945901/1‑150 (MQ=255)
                                                                                                                                             gCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGaa        >  1:3939554/1‑150 (MQ=255)
                                                                                                                                             gCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTACACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGaa        >  1:1703409/1‑150 (MQ=255)
                                                                                                                                              cccAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAAt       >  1:2990837/1‑150 (MQ=255)
                                                                                                                                              accAGGCCGCCAGCAGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAAt       >  1:2894751/2‑150 (MQ=255)
                                                                                                                                               ccAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAAtt      >  1:5319055/1‑150 (MQ=255)
                                                                                                                                                 aGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAATTCt    <  2:624062/150‑1 (MQ=255)
                                                                                                                                                 aGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAATTCt    >  2:5011530/1‑150 (MQ=255)
                                                                                                                                                 aGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAATTCt    >  2:336188/1‑150 (MQ=255)
                                                                                                                                                   gctgctGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAATTCTgc  >  1:416465/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTCGGCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAATTCTGC  >  NC_002947/1071138‑1071434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑