BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,249,325	C→T	6.2%	G39S (GGT→AGT) ‡	rnt ←	ribonuclease T

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,249,325	0	C	T	6.2%	806.2 / 28.5	256	G39S (GGT→AGT) ‡	rnt	ribonuclease T
Reads supporting (aligned to +/- strand): ref base C (134/106); new base T (3/13); total (137/119)
Fisher's exact test for biased strand distribution p-value = 4.34e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 281 total aligned reads displayed.

TCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGTTTTTC  >  NC_002947/1249177‑1249474

tCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCg                                                                                                                                                      >  1:4993734/1‑150 (MQ=255)

   aGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGtt                                                                                                                                                   >  1:2183121/1‑150 (MQ=255)

     tGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGATCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTc                                                                                                                                                 <  1:2736522/150‑1 (MQ=255)

       cggccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCGGTGGTTTCaa                                                                                                                                               <  2:963436/150‑1 (MQ=255)

       cggccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCaa                                                                                                                                               >  1:2693085/1‑150 (MQ=255)

        ggccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCACTTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAAc                                                                                                                                              >  2:588436/1‑150 (MQ=255)

         gccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCGGTGGTTTCAACa                                                                                                                                             <  2:3559058/150‑1 (MQ=255)

         gccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCGGTGGTTTCAACa                                                                                                                                             <  1:3246104/150‑1 (MQ=255)

         gccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACa                                                                                                                                             <  1:1035666/150‑1 (MQ=255)

           cggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATc                                                                                                                                           <  1:2903577/150‑1 (MQ=255)

            ggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATcc                                                                                                                                          >  1:1007278/1‑150 (MQ=255)

              cTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATccac                                                                                                                                        >  1:4288069/1‑150 (MQ=255)

                         cgcCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCa                                                                                                                             <  1:1129736/150‑1 (MQ=255)

                         cgcCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCa                                                                                                                             <  2:237832/150‑1 (MQ=255)

                           cccTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGa                                                                                                                           <  2:939132/150‑1 (MQ=255)

                            ccTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCGTCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGAt                                                                                                                          <  1:898395/150‑1 (MQ=255)

                            ccTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGAt                                                                                                                          >  2:3696557/1‑150 (MQ=255)

                             cTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATa                                                                                                                         <  1:5579112/150‑1 (MQ=255)

                             cTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATa                                                                                                                         >  1:3707337/1‑150 (MQ=255)

                              tCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAg                                                                                                                        >  1:3850584/1‑150 (MQ=255)

                                  aCGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCcgc                                                                                                                    <  2:5015937/150‑1 (MQ=255)

                                      cTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGaa                                                                                                                <  2:3128476/150‑1 (MQ=255)

                                         cACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACAACCACCACGACCGGCAGATAGCCGCGGAAgcg                                                                                                             >  2:816889/1‑150 (MQ=255)

                                          aCTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAgcgc                                                                                                            >  1:1333578/1‑150 (MQ=255)

                                          aCTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAgcgc                                                                                                            >  1:2027498/1‑150 (MQ=255)

                                          aCTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCAATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAgcgc                                                                                                            >  1:5164534/1‑150 (MQ=255)

                                            tCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAAc                                                                                                                                              >  2:1163723/1‑114 (MQ=255)

                                            tCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTc                                                                                                          >  2:3942910/1‑150 (MQ=255)

                                            tCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTc                                                                                                          >  1:2888838/1‑150 (MQ=255)

                                              ggTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCgg                                                                                                        >  1:4713836/1‑150 (MQ=255)

                                              ggTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCgg                                                                                                        <  2:2150182/150‑1 (MQ=255)

                                              ggTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCgg                                                                                                        <  2:318318/150‑1 (MQ=255)

                                               gTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGc                                                                                                       >  1:2955974/1‑150 (MQ=255)

                                                     gTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCgg                                                                                                 <  1:1200542/150‑1 (MQ=255)

                                                      tAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCggg                                                                                                <  2:1006539/150‑1 (MQ=255)

                                                          tATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGc                                                                                            <  2:2277755/150‑1 (MQ=255)

                                                          tATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGc                                                                                            <  1:863735/150‑1 (MQ=255)

                                                            tGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGa                                                                                          <  2:1232741/150‑1 (MQ=255)

                                                             gTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAg                                                                                         <  1:1601624/150‑1 (MQ=255)

                                                                    gAACAGGAAACCTTTCTCGTCCATGCTAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCgct                                                                                  >  2:2547347/1‑150 (MQ=255)

                                                                    gAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCgct                                                                                  >  1:5294558/1‑150 (MQ=255)

                                                                          gAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGcc                                                                            >  2:2495382/1‑150 (MQ=255)

                                                                          gAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGcc                                                                            >  1:1092523/1‑150 (MQ=255)

                                                                             aCCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTg                                                                         <  1:1887312/150‑1 (MQ=255)

                                                                              ccgttCTCGTCCATGCCAATGGTGACGGCGGCAATGTCCAGCAATGCATCGGGAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                        <  2:5215575/147‑1 (MQ=255)

                                                                              ccTTTCTCGTCCATGCCGATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                        <  1:4240928/150‑1 (MQ=255)

                                                                              ccTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                        <  1:4981788/150‑1 (MQ=255)

                                                                              ccTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                        <  1:4317764/150‑1 (MQ=255)

                                                                              ccTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                        <  2:1658350/150‑1 (MQ=255)

                                                                              ccTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                        <  2:2989822/150‑1 (MQ=255)

                                                                              ccTGTCTCGTCCATGCCGATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                        <  1:1402386/150‑1 (MQ=255)

                                                                                tttCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGgt                                                                      >  1:829998/1‑150 (MQ=255)

                                                                                tttCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGgt                                                                      >  1:4787781/1‑150 (MQ=255)

                                                                                  tctcGTCCAAGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGgtgt                                                                    >  1:3813317/1‑150 (MQ=255)

                                                                                    tcGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTcc                                                                  >  1:93688/1‑150 (MQ=255)

                                                                                    tcGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGCGTGGCGAGGACCGCTGCTGCCTTGGGTGTcc                                                                  >  1:2309621/1‑150 (MQ=255)

                                                                                      gTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAg                                                                <  1:1468593/150‑1 (MQ=255)

                                                                                        ccATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGACGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGgt                                                              >  1:3089311/1‑150 (MQ=255)

                                                                                           tGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGgtcgt                                                           >  1:616417/1‑150 (MQ=255)

                                                                                            gCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGgtcgtc                                                          <  2:789789/150‑1 (MQ=255)

                                                                                              cAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCtt                                                        >  1:5504838/1‑150 (MQ=255)

                                                                                              cAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCGTGGGTGTCCAGGTCGTCtt                                                        >  1:3543845/1‑150 (MQ=255)

                                                                                               aaTGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTc                                                       <  2:1868050/150‑1 (MQ=255)

                                                                                                aTGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCg                                                      <  2:322064/150‑1 (MQ=255)

                                                                                                 tGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGt                                                     >  2:4523134/1‑150 (MQ=255)

                                                                                                 tGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGt                                                     <  1:3345597/150‑1 (MQ=255)

                                                                                                   gTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTaa                                                   <  1:1264091/150‑1 (MQ=255)

                                                                                                      aCGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAgg                                                >  1:3241571/1‑150 (MQ=255)

                                                                                                        ggcggcAATTTCCAGCAATGCATCGGTAGCGCTGTGGAACCCCCCGGTGTCAACATCCACCACGACCGGCAGATAGCCGCGGACGCGCTCGGCCAGCGGGTGGCGAGGACCGCTGCTCCCTAGGGGGTCCAGGGCGTCTTCGTCAACGTc                                              >  2:1746096/1‑150 (MQ=255)

                                                                                                           ggcAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTc                                           <  1:238144/150‑1 (MQ=255)

                                                                                                                tttCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCa                                      >  2:5351655/1‑150 (MQ=255)

                                                                                                                   ccAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCAcgc                                   >  1:4559697/1‑150 (MQ=255)

                                                                                                                    cAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCAcgcg                                  >  2:1484072/1‑150 (MQ=255)

                                                                                                                         aTGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTGCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGttttt                             <  2:1965377/150‑1 (MQ=255)

                                                                                                                             aTCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTcc                         <  2:1351974/150‑1 (MQ=255)

                                                                                                                             aTCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTcc                         >  1:3190678/1‑150 (MQ=255)

                                                                                                                                ggTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                        >  2:546143/1‑100 (MQ=255)

                                                                                                                                ggTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCcagc                      >  1:5443976/1‑150 (MQ=255)

                                                                                                                                 gTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCcagca                     >  2:3862404/1‑150 (MQ=255)

                                                                                                                                    gcgcTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGgc                  <  1:5318147/150‑1 (MQ=255)

                                                                                                                                     cgcTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGgcg                 >  1:2342941/1‑150 (MQ=255)

                                                                                                                                      gcTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGgcgc                >  1:2397831/1‑150 (MQ=255)

                                                                                                                                        tGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCa              >  1:4173901/1‑150 (MQ=255)

                                                                                                                                         gTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAg             <  2:4348601/150‑1 (MQ=255)

                                                                                                                                          ttGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAgc            >  1:2753344/1‑150 (MQ=255)

                                                                                                                                          ttGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAgc            >  2:5408562/1‑150 (MQ=255)

                                                                                                                                          ttGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAgc            >  2:4868885/1‑150 (MQ=255)

                                                                                                                                            gAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAgcgc          >  1:4135001/1‑150 (MQ=255)

                                                                                                                                            gAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAgcgc          >  2:2258406/1‑150 (MQ=255)

                                                                                                                                             aaaCCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCa         <  2:2735628/150‑1 (MQ=255)

                                                                                                                                              aaCCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCGTGGGTGTCCAGGTCGTCTTCGTAAAGGACTTCGCTCACGCGTTATTCTCCAGCAGGCGCCAGCGCAg        >  2:3522503/1‑150 (MQ=255)

                                                                                                                                               accaccGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGt       >  2:4594081/1‑150 (MQ=255)

                                                                                                                                                 caccGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGttt     >  1:5480294/1‑150 (MQ=255)

                                                                                                                                                 caccGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGttt     >  2:648211/1‑150 (MQ=255)

                                                                                                                                                 caccGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGttt     >  1:1169369/1‑150 (MQ=255)

                                                                                                                                                 cacaGGTTTAAAAATCCAACACGAAAGGAAGATAGCAGCTGAATCGCTCGGACATCGGTTGGCGAGGAACGCTGCTGCATTGGGTGTCCAGGTCGTCTTCTTAAATGTCTTCGCTCACGCGTTTTTATCCAGCAGGCGCCAGCGCAGttt     >  2:40803/1‑150 (MQ=255)

                                                                                                                                                  accGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGtttt    >  2:4939304/1‑150 (MQ=255)

                                                                                                                                                    cGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGTTTTTc  >  2:1297281/1‑150 (MQ=255)

                                                                                                                                                    cGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGTTTTTc  <  2:2940002/150‑1 (MQ=255)

                                                                                                                                                    cGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGTTTTTc  >  1:459797/1‑150 (MQ=255)

TCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGTTTTTC  >  NC_002947/1249177‑1249474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`