BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,261,839	A→T	7.0%	noncoding (431/451 nt)	PP_mr14 ←	cspA regulatory region

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,261,839	0	A	T	7.0%	932.0 / 37.2	298	noncoding (431/451 nt)	PP_mr14	cspA regulatory region
Reads supporting (aligned to +/- strand): ref base A (143/131); new base T (12/10); total (155/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

CATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCTTTGA  >  NC_002947/1261693‑1261988

cATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGc                                                                                                                                                    >  2:2623908/1‑150 (MQ=255)

  tCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCgg                                                                                                                                                  <  1:3679378/150‑1 (MQ=255)

    aGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACAGCGCCGGACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCCGCGCCTATTACGAGCAGGCATAAAAATTCCCGCCAGAAGGCGGGt                                                                                                                                                >  1:497882/1‑150 (MQ=38)

     gCGAAGACTGACCTGCCATACCGAGTGTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGtt                                                                                                                                               <  1:5092866/150‑1 (MQ=255)

       gAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGtttt                                                                                                                                             <  1:1078710/150‑1 (MQ=255)

       gAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGtttt                                                                                                                                             >  2:2904278/1‑150 (MQ=255)

       gAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGtttt                                                                                                                                             >  1:247837/1‑150 (MQ=255)

        aaGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttt                                                                                                                                            >  1:1359298/1‑150 (MQ=255)

          gACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttttt                                                                                                                                          <  1:3269987/150‑1 (MQ=255)

          gACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttttt                                                                                                                                          <  1:5170975/150‑1 (MQ=255)

             tGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGTGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGaa                                                                                                                                       >  1:2450829/1‑150 (MQ=255)

             tGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGaa                                                                                                                                       >  2:3196396/1‑150 (MQ=255)

             tGACCTGCCAGACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGaa                                                                                                                                       <  2:4634897/150‑1 (MQ=255)

              gACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGaaa                                                                                                                                      <  2:2465515/150‑1 (MQ=255)

               aCCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCACAACAAAACCCGCCTTCTGGCGGGtttttttatgcc                                                                                                                                     <  1:1724637/150‑6 (MQ=38)

                  tGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAATCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTa                                                                                                                                  >  2:3062184/1‑150 (MQ=255)

                  tGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTa                                                                                                                                  >  2:568619/1‑150 (MQ=255)

                  tGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTa                                                                                                                                  <  1:1023516/150‑1 (MQ=255)

                          cgcgTTCGCCCGCACACAGCGACTGCGCCGTACTTGACGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTCTATCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTa                                                                                                                          <  2:3042894/147‑1 (MQ=39)

                          cGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTa                                                                                                                          <  2:2245772/150‑1 (MQ=255)

                             gTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGAt                                                                                                                       <  1:2158025/150‑1 (MQ=255)

                                tGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGa                                                                                                                    <  1:2561226/150‑1 (MQ=255)

                                 gCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGAc                                                                                                                   >  2:4051886/1‑150 (MQ=255)

                                   gctcccacagggactgcacaggcttcaagtacggcgcagtcgctgtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACtt                                                                                                                 >  1:4265660‑M2/48‑150 (MQ=255)

                                   ccGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACtt                                                                                                                 >  1:1970890/1‑150 (MQ=255)

                                   ccGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACtt                                                                                                                 >  1:1833904/1‑150 (MQ=255)

                                   ccGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACtt                                                                                                                 >  1:1667803/1‑150 (MQ=255)

                                     gCACACAGCGACTGCGCCGTTCTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCTAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAg                                                                                                               <  1:4520579/150‑1 (MQ=255)

                                     gCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAg                                                                                                               <  2:4205944/150‑1 (MQ=255)

                                       ccacagggactgcacaggcttcaagtacggcgcagtcgctgtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGc                                                                                                             >  1:4212746‑M2/44‑150 (MQ=255)

                                       acacaGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGc                                                                                                             >  2:3621957/1‑150 (MQ=255)

                                       acacaGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGc                                                                                                             >  1:1899947/1‑150 (MQ=255)

                                       acacaGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGGGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGc                                                                                                             <  1:4826126/150‑1 (MQ=255)

                                         ACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGa                                                                                                           >  1:3255623‑M1/1‑150 (MQ=255)

                                            GCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCt                                                                                                        <  1:3897700‑M1/150‑1 (MQ=255)

                                            GCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCt                                                                                                        <  2:715564‑M1/150‑1 (MQ=255)

                                             cGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTg                                                                                                       >  2:5507495/1‑150 (MQ=255)

                                              gACTGCGCCGTACTTGAAGCCTGTGCTGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGa                                                                                                      <  2:168239/150‑1 (MQ=255)

                                              gACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCCAGATCGCCTAAGTCGATCtag                                                                                                      <  1:850319/150‑3 (MQ=255)

                                                 tGCGCCGTACTTGAAGCCTATGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTAAGGCGATCTGAAct                                                                                                   >  2:4001386/1‑150 (MQ=255)

                                                    gcCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGAt                                                                                                          >  2:3281739/1‑140 (MQ=255)

                                                      cGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACttcttc                                                                                              <  1:3562462/150‑1 (MQ=255)

                                                       gTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCa                                                                                             <  1:4562185/150‑1 (MQ=255)

                                                       gTACTGGCAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCa                                                                                             <  2:3747259/150‑1 (MQ=255)

                                                          cTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGcc                                                                                          <  1:5459139/150‑1 (MQ=255)

                                                           ttGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCt                                                                                         >  2:2178528/1‑150 (MQ=255)

                                                                gCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATg                                                                                    >  2:4966476/1‑150 (MQ=255)

                                                                gCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATg                                                                                    >  2:1160110/1‑150 (MQ=255)

                                                                  cTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGcc                                                                                  >  2:179656/1‑150 (MQ=255)

                                                                     tgCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCttt                                                                               <  1:3707216/150‑1 (MQ=255)

                                                                      gCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTc                                                                              <  2:2390215/150‑1 (MQ=255)

                                                                      gCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTc                                                                              >  2:5601011/1‑150 (MQ=255)

                                                                      gCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTc                                                                              <  1:2640050/150‑1 (MQ=255)

                                                                          tCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGc                                                                          >  1:785159/1‑150 (MQ=255)

                                                                          tCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGc                                                                          >  1:9332/1‑150 (MQ=255)

                                                                            ccTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcg                                                                        >  2:474157/1‑150 (MQ=255)

                                                                              tgtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcgcg                                                                      >  2:4083616‑M2/5‑150 (MQ=255)

                                                                              tgtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcgcg                                                                      <  2:4482881‑M2/146‑1 (MQ=255)

                                                                               gtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCgg                                                                     <  1:541603‑M2/147‑1 (MQ=255)

                                                                                   gAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGACGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAg                                                                 <  2:3453559/150‑1 (MQ=255)

                                                                                     gCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGcg                                                               <  1:2213438/150‑1 (MQ=255)

                                                                                      cGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGcga                                                              <  1:3763350/150‑1 (MQ=255)

                                                                                           gAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGaaa                                                         <  2:5581030/150‑1 (MQ=255)

                                                                                           gAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGATTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGaaa                                                         <  2:2624604/150‑1 (MQ=255)

                                                                                            aGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAg                                                        <  2:1653544/150‑1 (MQ=255)

                                                                                                ctcgAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAAc                                                    <  1:3815186/148‑1 (MQ=255)

                                                                                                cgcgAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAAc                                                    >  2:443366/1‑150 (MQ=255)

                                                                                                 gcgAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTGTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCGTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACa                                                   >  2:5005445/1‑150 (MQ=255)

                                                                                                    aaGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGcc                                                >  1:1167960/1‑150 (MQ=255)

                                                                                                     agagGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCt                                               <  2:1956788/150‑1 (MQ=255)

                                                                                                      gagGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTg                                              <  2:5114033/150‑1 (MQ=255)

                                                                                                      gagGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTg                                              >  1:3127207/1‑150 (MQ=255)

                                                                                                         gCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGAGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGa                                                                                                                    <  2:3112464/77‑1 (MQ=255)

                                                                                                          ccAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCct                                          <  2:1359298/150‑1 (MQ=255)

                                                                                                           cAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCctt                                         >  1:2034546/1‑150 (MQ=255)

                                                                                                           cAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCctt                                         >  1:1010147/1‑150 (MQ=255)

                                                                                                             gcgcCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCcttct                                       <  2:247837/150‑1 (MQ=255)

                                                                                                                 cTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCa                                   <  2:3926269/150‑1 (MQ=255)

                                                                                                                 cTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCa                                   <  1:4051886/150‑1 (MQ=255)

                                                                                                                 cTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCa                                   <  2:637769/150‑1 (MQ=255)

                                                                                                                 cTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCa                                   >  1:1944122/1‑150 (MQ=255)

                                                                                                                  tATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGTTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAg                                  <  1:2867778/150‑1 (MQ=255)

                                                                                                                  tATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAg                                  <  1:3449335/150‑1 (MQ=255)

                                                                                                                    tttCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTGTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTGTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAggg                                >  2:1457562/1‑150 (MQ=255)

                                                                                                                      tCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTCGCGACGAAGGTAACAGCCTGGCCTTCTTTCGGGGtt                              >  2:5314607/1‑150 (MQ=255)

                                                                                                                        gAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGtttt                            <  1:4331466/150‑1 (MQ=255)

                                                                                                                              tttcaaaaaaaCCCGCCTTCTGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGcc                      >  1:1032068/5‑150 (MQ=255)

                                                                                                                              gCATACAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGcc                      <  1:4630120/150‑1 (MQ=255)

                                                                                                                                aTAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACttcttc                                                                                              <  1:5215894/76‑1 (MQ=255)

                                                                                                                                aTAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGt                    >  1:3005238/1‑150 (MQ=255)

                                                                                                                                    aaaaaCCCGCCTTCTGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGc                >  2:1892325/1‑150 (MQ=255)

                                                                                                                                        aCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTgg            <  2:197588/150‑1 (MQ=255)

                                                                                                                                         cccGCCAGAAGGCGGGTTTTTTTGAACGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGa           <  2:825959/150‑1 (MQ=255)

                                                                                                                                          ccGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGAt          <  2:2459233/150‑1 (MQ=255)

                                                                                                                                            gCCAGAAGGCTTGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATgg        <  1:2913849/150‑1 (MQ=255)

                                                                                                                                             ccAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGc       <  1:4175950/150‑1 (MQ=255)

                                                                                                                                             ccAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGc       <  1:2623908/150‑1 (MQ=255)

                                                                                                                                             ccAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGc       >  1:3223011/1‑150 (MQ=255)

                                                                                                                                              cAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCt      >  2:4826164/1‑150 (MQ=255)

                                                                                                                                                gAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCttt    <  2:1088405/150‑1 (MQ=255)

CATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCTTTGA  >  NC_002947/1261693‑1261988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`