BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,289,982	T→C	5.3%	G86G (GGT→GGC)	PP_1122 →	OmpA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,289,982	0	T	C	5.3%	910.2 / 23.9	281	G86G (GGT→GGC)	PP_1122	OmpA family protein
Reads supporting (aligned to +/- strand): ref base T (140/125); new base C (5/10); total (146/135)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

Only 100 of 296 total aligned reads displayed.

TGGTGGCTGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACA  >  NC_002947/1289834‑1290131

 ggtggCTGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCggtgg                                                                                                                                                     >  1:2430300/1‑150 (MQ=255)

  gtggCTGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCggtggt                                                                                                                                                    <  2:4205317/150‑1 (MQ=255)

    ggCTGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGtggtgg                                                                                                                                                  <  1:5087376/150‑1 (MQ=255)

       tGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTTCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGgcgc                                                                                                                                               >  2:4843433/1‑150 (MQ=255)

       tGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGgcgc                                                                                                                                               >  1:1245438/1‑150 (MQ=255)

       tGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGgcgc                                                                                                                                               >  2:345726/1‑150 (MQ=255)

       tGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGgcgc                                                                                                                                               <  1:5184363/150‑1 (MQ=255)

       tGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTAAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGgcgc                                                                                                                                               >  2:3603392/1‑150 (MQ=255)

          cAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCtt                                                                                                                                            >  1:4473426/1‑150 (MQ=255)

             ccTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTggg                                                                                                                                         <  2:2850079/150‑1 (MQ=255)

                  tCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCa                                                                                                                                    <  1:3811801/150‑1 (MQ=255)

                  tCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCa                                                                                                                                    >  1:1503187/1‑150 (MQ=255)

                  tCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCa                                                                                                                                    >  1:4375880/1‑150 (MQ=255)

                  tCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCa                                                                                                                                    >  1:181369/1‑150 (MQ=255)

                    aCGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCCtt                                                                                                                                  >  1:215292/1‑150 (MQ=255)

                      gggCTGCGCTACGCACAGCGATGGCAGCGCGACCCTCATTCAAAGGACCTGCCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGGCGGGGGTGGCGCCTTGGGCGGCCTTGc                                                                                                                                >  1:114502/1‑150 (MQ=255)

                        gCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGCAATGGCGCCCAAGGCGCCaccaccg                                                                                                                              <  1:5297053/150‑7 (MQ=255)

                         cTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGACGGCAATGGCGCCCAAGGCGCCaccaccgg                                                                                                                             <  2:515412/150‑8 (MQ=255)

                            ccgatctcacaGCGATGGCAGCGCGCCCCTCCATCAACGGACCGGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCcggcgg                                                                                                                          <  2:2515806/143‑1 (MQ=255)

                              cTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGc                                                                                                                        <  1:2243342/150‑1 (MQ=255)

                               tACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCt                                                                                                                       >  1:2083099/1‑150 (MQ=255)

                               tACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCt                                                                                                                       >  1:1608946/1‑150 (MQ=255)

                                aCGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGCAATGGCGCCCAAGGCGCCACCACcggcggccca                                                                                                                      <  1:3950677/150‑5 (MQ=37)

                                  gCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAt                                                                                                                    <  2:4724275/150‑1 (MQ=255)

                                    acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                  <  1:2347954/150‑1 (MQ=255)

                                    acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                  <  1:3032211/150‑1 (MQ=255)

                                    acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                  <  1:4820662/150‑1 (MQ=255)

                                    acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                  >  1:5413825/1‑150 (MQ=255)

                                    acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                  <  1:1351842/150‑1 (MQ=255)

                                      aGCGATGGCAGCGCGCCCCTCAATCACCGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGGGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGt                                                                                                                <  1:5265315/150‑1 (MQ=255)

                                      aGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGt                                                                                                                <  1:5567598/150‑1 (MQ=255)

                                      aGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGt                                                                                                                <  1:345726/150‑1 (MQ=255)

                                       gCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGtt                                                                                                               >  2:1186252/1‑150 (MQ=255)

                                            ggCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGcc                                                                                                          >  1:2868221/1‑150 (MQ=255)

                                             gCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGccc                                                                                                         <  1:849943/150‑1 (MQ=255)

                                              cAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCa                                                                                                        <  2:2198470/150‑1 (MQ=255)

                                              cAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCa                                                                                                        <  1:4858542/150‑1 (MQ=255)

                                               aGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAg                                                                                                       <  1:4592954/150‑1 (MQ=255)

                                                    gcCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACgg                                                                                                  >  1:2602205/1‑150 (MQ=255)

                                                      cccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCCGGACGGTg                                                                                                >  2:1137357/1‑150 (MQ=255)

                                                       ccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCGTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGa                                                                                               >  2:3259072/1‑150 (MQ=255)

                                                        cTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTTGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGAc                                                                                              >  2:675987/1‑150 (MQ=255)

                                                        cTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGAc                                                                                              >  2:5213249/1‑150 (MQ=255)

                                                        cTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCGTGGGCGCCCTTGCCGGCGGGCGGATTTGTTACGCCCAGGACGGTGAc                                                                                              >  1:3050057/1‑150 (MQ=255)

                                                            atcaaAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAg                                                                                          <  2:1092899/150‑1 (MQ=255)

                                                             tcaaAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGa                                                                                         >  2:2407483/1‑150 (MQ=255)

                                                             tcaaAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGa                                                                                         <  1:1401886/150‑1 (MQ=255)

                                                                    aCCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGa                                                                                            >  1:4118534/1‑140 (MQ=255)

                                                                    aCCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGa                                                                                            <  2:4118406/140‑1 (MQ=255)

                                                                      cTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACgg                                                                                >  2:3684660/1‑150 (MQ=255)

                                                                         gCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAATATGGTGACGGCAt                                                                             >  1:1075417/1‑150 (MQ=255)

                                                                           ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATGGAGTGTTCTTCCTGGGCCGCCGTTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGATGATGGTGACGGCAttt                                                                           <  1:4648981/150‑1 (MQ=255)

                                                                           ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAttt                                                                           >  2:949483/1‑150 (MQ=255)

                                                                           ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAttt                                                                           >  2:338314/1‑150 (MQ=255)

                                                                           ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAttt                                                                           >  1:3116244/1‑150 (MQ=255)

                                                                           ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAttt                                                                           >  1:4139151/1‑150 (MQ=255)

                                                                             aTCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTc                                                                         <  2:1436021/150‑1 (MQ=255)

                                                                                       tgctgGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTGTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGGTGTTTTGTTACGCCCAGGAGGGTGTCGAAGATGGTGTCGGCATTTTCGAGCGGggcg                                                               >  2:3293655/1‑150 (MQ=255)

                                                                                       tgctgGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGcgcg                                                               >  2:4599813/1‑150 (MQ=255)

                                                                                        gctgGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGa                                                              <  1:1866165/150‑1 (MQ=255)

                                                                                           gggCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCa                                                           <  2:4233325/150‑1 (MQ=255)

                                                                                               ggcTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGc                                                       >  1:1704043/1‑150 (MQ=255)

                                                                                                gcTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCa                                                                                                        <  1:3395165/100‑1 (MQ=255)

                                                                                                gcTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGcc                                                      <  2:173244/150‑1 (MQ=255)

                                                                                                gcTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGcc                                                      <  2:913429/150‑1 (MQ=255)

                                                                                                 cTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGccc                                                     <  1:592603/150‑1 (MQ=255)

                                                                                                  ttGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGcccc                                                    <  2:5547776/150‑1 (MQ=255)

                                                                                                  ttGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGcccc                                                    <  2:1330426/150‑1 (MQ=255)

                                                                                                   tGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAttt                                                                           <  2:2925401/126‑1 (MQ=255)

                                                                                                      tCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCgaga                                                <  1:3594493/150‑1 (MQ=255)

                                                                                                       cGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCGTGGGCGCCAATGCCGCCCGGCTGATTTGTTACGCCCAGGACGGTGACGAATATGGTGACGGCAATTTAGTCCGGCGCGATCACAGCCCCGCGAc                                               >  1:2498710/1‑150 (MQ=255)

                                                                                                        ggtggtGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGTTGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGGGAcc                                              >  2:3106679/1‑150 (MQ=255)

                                                                                                         gtggtgGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGAccc                                             <  2:2659438/150‑1 (MQ=255)

                                                                                                           ggtggCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCg                                           <  1:3656937/150‑1 (MQ=255)

                                                                                                             tggCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGc                                         >  1:5479100/1‑150 (MQ=255)

                                                                                                               gCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCa                                       >  2:5394311/1‑150 (MQ=255)

                                                                                                                  ttGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAaca                                    >  1:835026/1‑150 (MQ=255)

                                                                                                                     gTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACAcgg                                 >  1:2778482/1‑150 (MQ=255)

                                                                                                                         cATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGt                             <  1:3168337/150‑1 (MQ=255)

                                                                                                                          aTCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGtt                            >  2:5591476/1‑150 (MQ=255)

                                                                                                                             gagaGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGAc                         <  1:3724340/150‑1 (MQ=255)

                                                                                                                             gagaGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGAc                         >  2:1536627/1‑150 (MQ=255)

                                                                                                                             gagaGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACAGCCCCGAGACCCCGGCCAACACGGCGGTTGAc                         >  1:4926225/1‑150 (MQ=255)

                                                                                                                              agagTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGAcc                        >  1:4544953/1‑150 (MQ=255)

                                                                                                                              agagTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGAcc                        >  2:4890771/1‑150 (MQ=255)

                                                                                                                                  ttcttcCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACAt                    <  2:2104333/150‑1 (MQ=255)

                                                                                                                                    cttcCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATgg                  <  2:1879205/150‑1 (MQ=255)

                                                                                                                                     ttcCTGGGCCGCCGGTGGTAGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATggg                 >  2:2047011/1‑150 (MQ=255)

                                                                                                                                         tGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGc             >  1:3624760/1‑150 (MQ=255)

                                                                                                                                         tGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGc             >  2:2360987/1‑150 (MQ=255)

                                                                                                                                          gggCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGcc            <  2:400313/150‑1 (MQ=255)

                                                                                                                                          gggCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGcc            <  1:537987/150‑1 (MQ=255)

                                                                                                                                            gccgccGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCa          <  1:674906/150‑1 (MQ=255)

                                                                                                                                            cccgccgGCAATGGCGCCCAAGGCGCCACCACCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCa          >  2:4330287/2‑150 (MQ=38)

                                                                                                                                             ccgccgGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCAc         <  1:3377386/150‑1 (MQ=255)

                                                                                                                                              cgccgGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACt                                                         <  1:2555827/101‑1 (MQ=255)

                                                                                                                                              cgccgGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACt        <  1:192020/150‑1 (MQ=255)

                                                                                                                                              cgccgGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACt        <  2:2778482/150‑1 (MQ=255)

                                                                                                                                                    tggtggCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACa  <  2:4473426/150‑1 (MQ=255)

                                                                                                                                                    tggtggCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACa  <  2:4375880/150‑1 (MQ=255)

TGGTGGCTGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACA  >  NC_002947/1289834‑1290131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`