BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,385,679	T→A	5.5%	intergenic (‑112/+23)	PP_t67 ← / ← PP_t66	tRNA‑Met/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,385,679	0	T	A	5.5%	639.3 / 19.8	202	intergenic (‑112/+23)	PP_t67/PP_t66	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base T (111/79); new base A (5/6); total (116/86)
Fisher's exact test for biased strand distribution p-value = 5.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01

Only 100 of 205 total aligned reads displayed.

TATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTC  >  NC_002947/1385530‑1385816

tATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGtt                                                                                                                                           <  1:74762/150‑1 (MQ=255)

     gCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAg                                                                                                                                      >  1:2650893/1‑150 (MQ=255)

       ccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACCGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                    <  2:2495988/150‑1 (MQ=255)

         gacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGAGGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGc                                                                                                                                  >  2:1308652/1‑150 (MQ=255)

           cgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCt                                                                                                                                <  1:4332838/150‑1 (MQ=255)

            gaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGCCtt                                                                                                                               <  2:2106141/150‑1 (MQ=255)

               cTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTc                                                                                                                            >  1:4213277/1‑150 (MQ=255)

               cTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTc                                                                                                                            <  1:1524420/150‑1 (MQ=255)

               cTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTc                                                                                                                            >  1:3421858/1‑150 (MQ=255)

                 aCCAGGCTGCTCCAGCCCGCGACAGTGGGGCGGATTCTACAGGGTGTGGTTTGTATGTCAAGCGCTAGGCGTTGCTCTTTCGACGTTTGTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACGGTGTTAACAGTGGCCTTTTCtt                                                                                                                          <  2:3133336/150‑1 (MQ=18)

                   cAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGa                                                                                                                        <  2:4868773/150‑1 (MQ=255)

                     ggCTGCTCCAGCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAAt                                                                                                                      <  2:4658941/150‑1 (MQ=255)

                        tgctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTTGATATGAGGCGTTTGTTCTCGTGACCTTTTCACCAGGCAATGGTAAAGGCCAGTGTGTTAACTGTGGGCTTGTCTTggcggtg                                                                                                                   >  2:1271602/1‑144 (MQ=9)

                           tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGtt                                                                                                                >  1:1478388/1‑150 (MQ=255)

                           tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGATAATGCTTATCATCAGGCAAAGAAAATGGCAACTTTGTTAACAGTGGCATTTTATTGAATCTGGtt                                                                                                                >  1:3241330/1‑150 (MQ=17)

                             cATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGc                                                                                                              >  1:1353345/1‑150 (MQ=255)

                             cATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTGTCGACGTTTAATCTCGAATGCATATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGTCCTTTTCTTGATTCTGGTTGc                                                                                                              >  1:913943/1‑150 (MQ=18)

                              aTCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCg                                                                                                             >  2:5438709/1‑150 (MQ=255)

                               tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGATAATGCTTATCAGAATGCAAAGAAAAAGGCAACTGTGTTAACATTTGACTTTTCTTGAATCTGGTTGctg                                                                                                            >  1:628147/1‑148 (MQ=17)

                                 ccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAAAGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTGTCTTGAATCTGGTTGCGGGa                                                                                                          >  1:1877818/1‑150 (MQ=255)

                                  cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGAGGTTTTTTCGATAATGCTTATCAACAGGCAAAGAAAAAGGACACAGTGATAACAGAGTCAATTTCTAGAATCTCGTTGCGGGAg                                                                                                         >  2:5292959/1‑150 (MQ=14)

                                    cgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGcc                                                                                                       <  2:3087592/150‑1 (MQ=255)

                                         gTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAtt                                                                                                  <  2:2214253/150‑1 (MQ=255)

                                          tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                 <  1:3884767/150‑1 (MQ=255)

                                          tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAtt                                                                                                  >  1:2596222/1‑149 (MQ=255)

                                           ggggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTg                                                                                                <  1:5504601/150‑1 (MQ=255)

                                            gggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGa                                                                                               >  2:5185832/1‑150 (MQ=255)

                                            gggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGa                                                                                               >  2:1022844/1‑150 (MQ=255)

                                              gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTg                                                                                                                   >  1:1908357/1‑128 (MQ=255)

                                              gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTg                                                                                                                   <  2:1908357/128‑1 (MQ=255)

                                              gCGGAGTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGATTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTATCTTGAATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                             <  2:2279464/150‑1 (MQ=255)

                                                ggATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcg                                                                                           >  1:992176/1‑150 (MQ=255)

                                                   ttCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcgacg                                                                                        >  1:837993/1‑150 (MQ=255)

                                                     cTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCgacgac                                                                                      <  2:565811/150‑1 (MQ=255)

                                                      tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGCCtttttcttt                                                                                                                        >  1:4117082/1‑110 (MQ=21)

                                                      tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGAcc                                                                                     >  2:2757932/1‑150 (MQ=255)

                                                      tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGAcc                                                                                     >  1:655718/1‑150 (MQ=255)

                                                      tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATAAGCAGGCAAAGAAAAAGGCAACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCAGGATTTGAACCGACGAcc                                                                                     >  2:2591770/1‑150 (MQ=255)

                                                       aCAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCt                                                                                    >  2:2043565/1‑150 (MQ=255)

                                                       aCAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCt                                                                                    >  2:2883454/1‑150 (MQ=255)

                                                        cAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCtt                                                                                   >  1:5213740/1‑150 (MQ=255)

                                                         aGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTc                                                                                  <  2:1626546/150‑1 (MQ=255)

                                                         aGTGTGAGGTATGTATGTCAAGCGCTAGGCTTTGAGTTTTCGACGATTTGACGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCATGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTc                                                                                  <  2:5456423/150‑1 (MQ=18)

                                                          gtgtgtGGTTTGTATGTCAAGCGCTAGGCTGTGATTTTTCGACGTTTTTTCGCTAATGCTTATCAGCAGGCAAAGCAATAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCg                                                                                 <  2:4573639/150‑1 (MQ=255)

                                                           tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                <  1:5369341/150‑1 (MQ=255)

                                                           tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                >  2:858290/1‑150 (MQ=255)

                                                             tgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGt                                                                              <  2:4405080/150‑1 (MQ=255)

                                                               tgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGCCGTTTTTTCGCGTTTGCTTATCAGCAGGCATTGTTAAAGGCGACTGTGTTCAGTGTGGCGTTTTGTTGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTa                                                                            <  2:4052102/150‑1 (MQ=18)

                                                                 gTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATg                                                                          >  1:975639/1‑150 (MQ=255)

                                                                 gTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATg                                                                          <  2:4856829/150‑1 (MQ=255)

                                                                  tttGTATGTCAAGCGCGAGGCTTTGATTGATCGACGTTTTGTCTCGAAGTCTTATCAGCAGTCAATGAAAAAGGCCTCTGTGTTTAGAGTGGGCTTTTGTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGa                                                                         <  2:3576052/150‑1 (MQ=17)

                                                                      tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                     >  2:944384/1‑150 (MQ=255)

                                                                      tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                     <  1:5516878/150‑1 (MQ=255)

                                                                      tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAAGAGccc                                                                     >  1:3619721/1‑150 (MQ=255)

                                                                      tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGAAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                     >  2:1241105/1‑150 (MQ=255)

                                                                       aTGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcg                                                                    >  2:4932717/1‑150 (MQ=255)

                                                                         gTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCTCGAATGCTTATCAGCAGGCATAGATAAAGGCCACTGTTTTAACAGTGGCCTTTTATTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgac                                                                  <  1:2970260/150‑1 (MQ=17)

                                                                          tCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacg                                                                 >  2:29868/1‑150 (MQ=255)

                                                                           cAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacga                                                                >  2:611162/1‑150 (MQ=255)

                                                                            aaGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAg                                                               >  1:3547415/1‑150 (MQ=255)

                                                                             atcgcTAGGCTTTGATTTTTCGCCGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTTACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGc                                                              <  2:757766/148‑1 (MQ=255)

                                                                                 cTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAcc                                                          <  2:1606768/150‑1 (MQ=255)

                                                                                  tAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCa                                                         >  1:2823676/1‑150 (MQ=255)

                                                                                       tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctg                                                    >  1:645590/1‑150 (MQ=255)

                                                                                       tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctg                                                    >  2:2286301/1‑150 (MQ=255)

                                                                                        ttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctgc                                                   >  1:3491935/1‑150 (MQ=255)

                                                                                          gATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTACGGGTTATGAGCCCGACGAGCTACCAGGCTGCTc                                                 >  2:2689171/1‑150 (MQ=255)

                                                                                            tttttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCa                                               >  1:3666533/1‑150 (MQ=255)

                                                                                            tttttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCa                                               >  1:2129053/1‑150 (MQ=255)

                                                                                            tttttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCa                                               >  1:837145/1‑150 (MQ=255)

                                                                                             ttttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGTATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCAt                                              <  2:2389322/150‑1 (MQ=255)

                                                                                                   aCGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcgcg                                        >  1:4118931/1‑150 (MQ=255)

                                                                                                   aCGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCGGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcgcg                                        >  2:149431/1‑150 (MQ=255)

                                                                                                     gTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCAGACGGGCTAACAGCAAGCACCAACCCGAGTc                                      >  1:3071786/1‑150 (MQ=17)

                                                                                                          ttCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgg                                 >  1:3354740/1‑150 (MQ=255)

                                                                                                           tCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGaa                                                                                              <  1:3761007/88‑1 (MQ=255)

                                                                                                           tCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGaa                                                                                              >  2:3761007/1‑88 (MQ=255)

                                                                                                            tttgcctGCTGATAAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgggg                               >  2:660935/7‑150 (MQ=255)

                                                                                                            cgcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACTGTGGCCTTTTCTTGAATCTGGTTTCTGGAGCCGGATTTGATCCGACGAGCTTCTGGTTTTGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgggg                               <  2:5472930/150‑1 (MQ=17)

                                                                                                            cgcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCTGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgggg                               <  2:2650893/150‑1 (MQ=255)

                                                                                                             gcgAATGCTTATCAGCAGGCAAAGAAAATGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGc                              >  1:1665623/1‑150 (MQ=255)

                                                                                                                   gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTc                        <  2:3421858/150‑1 (MQ=255)

                                                                                                                     ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTa                      >  2:1220181/1‑150 (MQ=255)

                                                                                                                     ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTa                      >  1:208749/1‑150 (MQ=255)

                                                                                                                     ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTa                      >  1:5412867/1‑150 (MQ=255)

                                                                                                                             agGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCtt                                                                                   >  2:5027702/1‑81 (MQ=39)

                                                                                                                             agGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCgtgt              >  1:107214/1‑150 (MQ=255)

                                                                                                                             agGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTCGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                <  1:5454334/84‑1 (MQ=14)

                                                                                                                                 aaaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctgc                                                   <  2:1049273/109‑1 (MQ=35)

                                                                                                                                 aaaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctgc                                                   >  1:1049273/1‑109 (MQ=35)

                                                                                                                                    gAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                >  1:4323977/1‑77 (MQ=39)

                                                                                                                                    gAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacga                                                                >  2:555924/1‑93 (MQ=37)

                                                                                                                                    gAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTAGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                <  2:1487688/77‑1 (MQ=21)

                                                                                                                                     gaaaaGGCCACTGTTAACACAGTGGCCTTTTTTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCATCGGGTTATGACCCCGAACAGCAACCAGCCTGCTCCATACACCGTAGGGGGGGCGGAATCTACAGCGTGGAGGTtatt      >  1:4072016/2‑149 (MQ=9)

                                                                                                                                     aaaaaGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACTACCTTCTGGTTATTAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtata      <  2:4213277/150‑1 (MQ=255)

                                                                                                                                         aGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTc  <  1:2883454/150‑1 (MQ=255)

                                                                                                                                             cACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCt                                                             >  1:1418025/1‑87 (MQ=38)

                                                                                                                                                tgtgTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                           >  1:1190509/1‑70 (MQ=40)

                                                                                                                                                tgtgTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGt   >  1:1003894/1‑142 (MQ=255)

                                                                                                                                                    ttAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                           >  2:3662463/1‑66 (MQ=35)

TATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTC  >  NC_002947/1385530‑1385816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`