BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,385,680	A→C	5.2%	intergenic (‑113/+22)	PP_t67 ← / ← PP_t66	tRNA‑Met/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,385,680	0	A	C	5.2%	647.7 / 14.5	204	intergenic (‑113/+22)	PP_t67/PP_t66	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base A (112/76); new base C (5/7); total (117/87)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

GCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAGGCTT  >  NC_002947/1385535‑1385829

gCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTGTGTATGTCAAGCGCTAGGCTTTGAGTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAg                                                                                                                                                   >  1:5472930/1‑150 (MQ=255)

  ccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACCGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                                 <  2:2495988/150‑1 (MQ=255)

  ccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                                 <  1:5379785/150‑1 (MQ=255)

   cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAG‑AAAAGGCCACTGTTAACACAGTGGc                                                                                                                                               <  1:1396053/150‑1 (MQ=18)

    gacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGAGGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGc                                                                                                                                               >  2:1308652/1‑150 (MQ=255)

      cgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCt                                                                                                                                             <  1:4332803/150‑1 (MQ=255)

      cgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCt                                                                                                                                             <  1:1898865/150‑1 (MQ=255)

       gaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGCCtt                                                                                                                                            <  2:2106141/150‑1 (MQ=255)

         gCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGAATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCtttt                                                                                                                                          <  1:1982103/150‑1 (MQ=255)

          cTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTc                                                                                                                                         <  1:2090860/150‑1 (MQ=255)

            aCCAGGCTGCTCCAGCCCGCGACAGTGGGGCGGATTCTACAGGGTGTGGTTTGTATGTCAAGCGCTAGGCGTTGCTCTTTCGACGTTTGTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACGGTGTTAACAGTGGCCTTTTCtt                                                                                                                                       <  2:3133336/150‑1 (MQ=18)

              cAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGCCtttttcttt                                                                                                                                     <  1:2858584/150‑6 (MQ=18)

              cAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGa                                                                                                                                     <  2:4868773/150‑1 (MQ=255)

               aGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGaa                                                                                                                                    <  1:3516991/150‑1 (MQ=255)

                ggCTGCTCCAGCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAAt                                                                                                                                   <  2:4658941/150‑1 (MQ=255)

                   tgctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTTGATATGAGGCGTTTGTTCTCGTGACCTTTTCACCAGGCAATGGTAAAGGCCAGTGTGTTAACTGTGGGCTTGTCTTggcggtg                                                                                                                                >  2:1271602/1‑144 (MQ=9)

                    gctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTCGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTgg                                                                                                                               <  1:5277937/150‑1 (MQ=255)

                    gctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTgg                                                                                                                               <  1:2453671/150‑1 (MQ=255)

                        cATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAATGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGc                                                                                                                           >  1:3084267/1‑150 (MQ=255)

                        cATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTGTCGACGTTTAATCTCGAATGCATATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGTCCTTTTCTTGATTCTGGTTGc                                                                                                                           >  1:913943/1‑150 (MQ=18)

                         aTCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCg                                                                                                                          >  2:5438709/1‑150 (MQ=255)

                             cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGAGGTTTTTTCGATAATGCTTATCAACAGGCAAAGAAAAAGGACACAGTGATAACAGAGTCAATTTCTAGAATCTCGTTGCGGGAg                                                                                                                      >  2:5292959/1‑150 (MQ=14)

                              gcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGc                                                                                                                     >  1:4228522/1‑150 (MQ=255)

                               cgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGcc                                                                                                                    <  2:3087592/150‑1 (MQ=255)

                                 tCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCgg                                                                                                                  >  1:4202384/1‑150 (MQ=255)

                                    gTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAtt                                                                                                               <  2:2214253/150‑1 (MQ=255)

                                     tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                              <  1:3884510/150‑1 (MQ=255)

                                       gggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGa                                                                                                            >  2:5185832/1‑150 (MQ=255)

                                       gggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGa                                                                                                            >  2:1022844/1‑150 (MQ=255)

                                        ggCGGAGTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGaa                                                                                                           <  1:1654430/150‑1 (MQ=255)

                                         gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTg                                                                                                                                <  2:1908357/128‑1 (MQ=255)

                                         gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                                          <  1:5447024/150‑1 (MQ=255)

                                         gCGGAGTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGATTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTATCTTGAATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                                          <  2:2279464/150‑1 (MQ=255)

                                           ggATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcg                                                                                                        >  1:992176/1‑150 (MQ=255)

                                            gATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcga                                                                                                       <  1:3695842/150‑1 (MQ=255)

                                              ttCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcgacg                                                                                                     >  1:837993/1‑150 (MQ=255)

                                                cTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCgacgac                                                                                                   <  2:565811/150‑1 (MQ=255)

                                                cTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCgacgac                                                                                                   >  1:635000/1‑150 (MQ=255)

                                                 tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGAcc                                                                                                  >  2:2757932/1‑150 (MQ=255)

                                                 tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGAcc                                                                                                  >  1:655737/1‑150 (MQ=255)

                                                 tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATAAGCAGGCAAAGAAAAAGGCAACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCAGGATTTGAACCGACGAcc                                                                                                  >  2:2591770/1‑150 (MQ=255)

                                                  aCAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCt                                                                                                 >  2:2883454/1‑150 (MQ=255)

                                                  aCAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCt                                                                                                 >  2:2043565/1‑150 (MQ=255)

                                                    aGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTc                                                                                               <  2:1626546/150‑1 (MQ=255)

                                                    aGTGTGAGGTATGTATGTCAAGCGCTAGGCTTTGAGTTTTCGACGATTTGACGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCATGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTc                                                                                               <  2:5456423/150‑1 (MQ=18)

                                                     gtgtgtGGTTTGTATGTCAAGCGCTATGCTTTGATTTTTCGACGTTATTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTACCAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCg                                                                                              <  1:388544/150‑1 (MQ=255)

                                                     gtgtgtGGTTTGTATGTCAAGCGCTAGGCTGTGATTTTTCGACGTTTTTTCGCTAATGCTTATCAGCAGGCAAAGCAATAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCg                                                                                              <  2:4573639/150‑1 (MQ=255)

                                                      tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                             >  2:858290/1‑150 (MQ=255)

                                                        tgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGt                                                                                           <  2:4405080/150‑1 (MQ=255)

                                                         gtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGtt                                                                                          >  1:2615947/1‑150 (MQ=255)

                                                          tgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGCCGTTTTTTCGCGTTTGCTTATCAGCAGGCATTGTTAAAGGCGACTGTGTTCAGTGTGGCGTTTTGTTGAATATGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTa                                                                                         <  2:4052102/150‑1 (MQ=18)

                                                           ggTTTGTATGTCAAGCGCTAGGCTTTTATTTTTCGAAGTTTTTTGGCGATTGCTTTTCAGCAGGCAAATAAATAGGGCACTGTTTTAACCGGTGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                        <  1:1863584/150‑1 (MQ=17)

                                                            gTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATg                                                                                       >  1:975639/1‑150 (MQ=255)

                                                            gTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATg                                                                                       <  2:4856829/150‑1 (MQ=255)

                                                             tttGTATGTCAAGCGCGAGGCTTTGATTGATCGACGTTTTGTCTCGAAGTCTTATCAGCAGTCAATGAAAAAGGCCTCTGTGTTTAGAGTGGGCTTTTGTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGa                                                                                      <  2:3576052/150‑1 (MQ=17)

                                                                 tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                                  >  2:944384/1‑150 (MQ=255)

                                                                 tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGAAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                                  >  2:1241105/1‑150 (MQ=255)

                                                                  aTGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcg                                                                                 >  2:4932717/1‑150 (MQ=255)

                                                                     tCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacg                                                                              >  2:29868/1‑150 (MQ=255)

                                                                      cAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacga                                                                             >  2:611162/1‑150 (MQ=255)

                                                                        atcgcTAGGCTTTGATTTTTCGCCGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTTACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGc                                                                           <  2:757766/148‑1 (MQ=255)

                                                                        aGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGc                                                                           >  1:328132/1‑150 (MQ=255)

                                                                            cTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAcc                                                                       <  2:1606768/150‑1 (MQ=255)

                                                                               ggCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAgg                                                                    <  1:2957037/150‑1 (MQ=255)

                                                                                  tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctg                                                                 >  2:2286301/1‑150 (MQ=255)

                                                                                  tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctg                                                                 >  1:645599/1‑150 (MQ=255)

                                                                                     gATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTACGGGTTATGAGCCCGACGAGCTACCAGGCTGCTc                                                              >  2:2689171/1‑150 (MQ=255)

                                                                                       tttttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCa                                                            >  1:837145/1‑150 (MQ=255)

                                                                                        ttttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGTATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCAt                                                           <  2:2389322/150‑1 (MQ=255)

                                                                                              aCGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcgcg                                                     >  1:4117885/1‑150 (MQ=255)

                                                                                              aCGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCGGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcgcg                                                     >  2:149431/1‑150 (MQ=255)

                                                                                                gTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTc                                                   >  1:568522/1‑150 (MQ=255)

                                                                                                gTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTc                                                   >  1:4434500/1‑150 (MQ=255)

                                                                                                     ttCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCAGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgg                                              >  1:3355130/1‑150 (MQ=255)

                                                                                                      tCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGaa                                                                                                           >  2:3761007/1‑88 (MQ=255)

                                                                                                       tttgcctGCTGATAAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgggg                                            >  2:660935/7‑150 (MQ=255)

                                                                                                       cgcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACTGTGGCCTTTTCTTGAATCTGGTTTCTGGAGCCGGATTTGATCCGACGAGCTTCTGGTTTTGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgggg                                            <  2:5472930/150‑1 (MQ=17)

                                                                                                       cgcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCTGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgggg                                            <  2:2650893/150‑1 (MQ=255)

                                                                                                          gAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGCGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCgg                                         >  1:3050737/1‑150 (MQ=255)

                                                                                                              gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTc                                     <  2:3421858/150‑1 (MQ=255)

                                                                                                                ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTa                                   >  2:1220181/1‑150 (MQ=255)

                                                                                                                 tATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGACAGTGTGGCGGATTCTAc                                  >  1:1435588/1‑150 (MQ=255)

                                                                                                                  aTCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCGGTGGGGGGGATTCTAcg                                 >  1:4101874/1‑149 (MQ=255)

                                                                                                                        agGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCtt                                                                                                >  2:5027702/1‑81 (MQ=39)

                                                                                                                        agGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCgtgt                           >  1:3474302/1‑150 (MQ=255)

                                                                                                                        agGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCgtgt                           >  1:107214/1‑150 (MQ=255)

                                                                                                                            aaaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctgc                                                                <  2:1049273/109‑1 (MQ=35)

                                                                                                                            aaaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctgc                                                                >  1:1049273/1‑109 (MQ=35)

                                                                                                                               gAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                             >  1:1487688/1‑77 (MQ=39)

                                                                                                                               gAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacga                                                                             >  2:555924/1‑93 (MQ=37)

                                                                                                                               gAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtat                    <  1:5516212/150‑1 (MQ=255)

                                                                                                                               gAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTAGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                             <  2:1487688/77‑1 (MQ=21)

                                                                                                                                gaaaaGGCCACTGTTAACACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtata                   >  1:3617466/2‑150 (MQ=255)

                                                                                                                                aaaaaGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACTACCTTCTGGTTATTAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtata                   <  2:4213277/150‑1 (MQ=255)

                                                                                                                                   aaGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGt                >  1:2687320/1‑150 (MQ=255)

                                                                                                                                           tgtgTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                        >  1:1190509/1‑70 (MQ=40)

                                                                                                                                           tgtgTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGt                >  1:1003894/1‑142 (MQ=255)

                                                                                                                                               ttAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                        >  2:3662463/1‑66 (MQ=35)

                                                                                                                                               ttAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                        <  1:3662463/66‑1 (MQ=35)

                                                                                                                                                 aaCAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCAGGATTTTAACCGTCGAGCTTGGTGTTATGAGCCCTTCGAGCTAGATGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAGGCtt  <  1:1271602/150‑1 (MQ=17)

GCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAGGCTT  >  NC_002947/1385535‑1385829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`