BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,554,891	C→T	5.3%	V100V (GTC→GTT)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,554,891	0	C	T	5.3%	838.2 / 22.2	261	V100V (GTC→GTT)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand): ref base C (112/135); new base T (3/11); total (115/146)
Fisher's exact test for biased strand distribution p-value = 9.96e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

AAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGTACCA  >  NC_002947/1554742‑1555039

aaGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTc                                                                                                                                                      >  1:4483069/1‑150 (MQ=255)

aaGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTc                                                                                                                                                      >  1:5175826/1‑150 (MQ=255)

  ggACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGTCATCGTCaa                                                                                                                                                    >  2:2929197/1‑150 (MQ=255)

   gACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAAc                                                                                                                                                   <  1:2211822/150‑1 (MQ=255)

   gACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAAc                                                                                                                                                   >  2:2464401/1‑150 (MQ=255)

    aCGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACg                                                                                                                                                  <  1:3403030/150‑1 (MQ=255)

     cGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCTTCGTTGACGa                                                                                                                                                 <  1:2571581/150‑1 (MQ=255)

      ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGaa                                                                                                                                                >  1:3085000/1‑150 (MQ=255)

         gTTTCCTTCGCCACAGAAAGCGAGCTTAAAGACGCTTTCGAAATAATGGGCTCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCATCGACGCTGCCGGTGACGGCTCCACCACCGCTACCGTTCTGGC‑‑TCAGGCCTTCGTTGACGATGGc                                                                                                                                             <  2:319785/150‑1 (MQ=255)

         gTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGc                                                                                                                                             <  2:1376026/150‑1 (MQ=255)

         gTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGc                                                                                                                                             <  2:4763274/150‑1 (MQ=255)

          tttCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCTTCGTTGACGATGGcc                                                                                                                                            <  1:500816/150‑1 (MQ=255)

           ttCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCt                                                                                                                                           <  1:1711458/150‑1 (MQ=255)

           ttCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCt                                                                                                                                           <  1:4381499/150‑1 (MQ=255)

             ccGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCTTCGTTGACGATGGCCTGa                                                                                                                                         <  2:3536371/150‑1 (MQ=255)

             ccGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCTTCGTTGACGATGGCCTGa                                                                                                                                         <  2:5119383/150‑1 (MQ=255)

              cGTCGCCACAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGaa                                                                                                                                        <  2:1791875/150‑1 (MQ=255)

                 cGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTTTCAGGCCTTCGTTGACGATGGCCTGagc                                                                                                                                       <  1:3219704/150‑3 (MQ=255)

                  gCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGcc                                                                                                                                    <  2:3096230/150‑1 (MQ=255)

                  gCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGcc                                                                                                                                    >  2:3481575/1‑150 (MQ=255)

                   ccAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCg                                                                                                                                   >  2:1928988/1‑150 (MQ=255)

                    cAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGt                                                                                                                                  >  2:2629497/1‑150 (MQ=255)

                     aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                                                                                                 >  2:4102457/1‑150 (MQ=255)

                     aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                                                                                                 >  1:3453394/1‑150 (MQ=255)

                      aaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCg                                                                                                                                <  2:4779406/150‑1 (MQ=255)

                                gCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCAt                                                                                                                      <  1:751751/150‑1 (MQ=255)

                                gCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCAt                                                                                                                      >  1:4269923/1‑150 (MQ=255)

                                  tGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGa                                                                                                                    >  1:4881430/1‑150 (MQ=255)

                                   gAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGaa                                                                                                                   >  1:4923381/1‑150 (MQ=255)

                                    aaaGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAAc                                                                                                                  <  1:845294/150‑1 (MQ=255)

                                     aaGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAAcc                                                                                                                 <  2:412458/150‑1 (MQ=255)

                                       gACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCg                                                                                                               >  2:4236567/1‑150 (MQ=255)

                                       gACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCg                                                                                                               >  2:1397381/1‑150 (MQ=255)

                                        aCGCTTTCGAAACCATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGACACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGa                                                                                                              <  1:289221/150‑1 (MQ=255)

                                        aCGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGa                                                                                                              >  2:5594013/1‑150 (MQ=255)

                                          gCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATg                                                                                                            <  1:4239742/150‑1 (MQ=255)

                                            tttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGa                                                                                                          >  2:2193312/1‑150 (MQ=255)

                                            tttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGa                                                                                                          >  2:3676449/1‑150 (MQ=255)

                                             ttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGcc                                                                                                                                    <  1:2807498/123‑1 (MQ=255)

                                             ttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGAc                                                                                                         >  1:1374428/1‑150 (MQ=255)

                                             ttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGAc                                                                                                         <  1:1609525/150‑1 (MQ=255)

                                               cGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCt                                                                                                       <  2:2899333/150‑1 (MQ=255)

                                                gAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTg                                                                                                      >  2:1642828/1‑150 (MQ=255)

                                                  aaaCATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGaa                                                                                                    >  1:5626819/1‑150 (MQ=255)

                                                     cATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcg                                                                                                 >  1:3047173/1‑150 (MQ=255)

                                                      aTGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcgc                                                                                                >  2:1832720/1‑150 (MQ=255)

                                                       tGGGCGCCCAGCTGGTCACGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                                                                                               <  1:3559906/150‑1 (MQ=255)

                                                       tGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                                                                                               <  2:2033970/150‑1 (MQ=255)

                                                        gggCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCgg                                                                                              >  1:1637016/1‑150 (MQ=255)

                                                          gcgcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTa                                                                                            >  1:104851/1‑150 (MQ=255)

                                                           cgcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTAt                                                                                           <  2:338630/150‑1 (MQ=255)

                                                                aGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACa                                                                                      >  1:5257051/1‑150 (MQ=255)

                                                                     gTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAg                                                                                                   >  2:1266597/1‑132 (MQ=255)

                                                                     gTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAg                                                                                                   <  1:1266597/132‑1 (MQ=255)

                                                                      tCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCa                                                                                <  2:4645337/150‑1 (MQ=255)

                                                                      tCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCa                                                                                >  2:60882/1‑150 (MQ=255)

                                                                        aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct                                                                              <  2:4327354/150‑1 (MQ=255)

                                                                        aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct                                                                              >  2:2780570/1‑150 (MQ=255)

                                                                        aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct                                                                              >  1:2059486/1‑150 (MQ=255)

                                                                        aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct                                                                              >  1:3399962/1‑150 (MQ=255)

                                                                               ttGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcg                                                                       >  2:4937596/1‑150 (MQ=255)

                                                                                  cTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGt                                                                                             >  2:4452287/1‑125 (MQ=255)

                                                                                   ttCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcgtcgt                                                                   >  1:2412259/1‑150 (MQ=255)

                                                                                   ttCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcgtcgt                                                                   >  1:5298503/1‑150 (MQ=255)

                                                                                      cAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGc                                                                <  2:2287405/150‑1 (MQ=255)

                                                                                      cAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGc                                                                <  2:5227217/150‑1 (MQ=255)

                                                                                       aaGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGcc                                                               >  2:775430/1‑150 (MQ=255)

                                                                                       aaGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGcc                                                               >  1:4501090/1‑150 (MQ=255)

                                                                                        aGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCg                                                              <  1:4023215/150‑1 (MQ=255)

                                                                                          gCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGaa                                                            <  1:5432089/150‑1 (MQ=255)

                                                                                           ccAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAAc                                                           <  2:2920205/150‑1 (MQ=255)

                                                                                            catcgacgCTGCCGGTGACGGCACCACCACCGCTTCCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACt                                                          <  2:5403446/147‑1 (MQ=255)

                                                                                             aaCGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTg                                                         >  1:4479946/1‑150 (MQ=255)

                                                                                             aaCGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTg                                                         >  1:68601/1‑150 (MQ=255)

                                                                                              acgacgCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTga                                                        <  1:2972954/150‑1 (MQ=255)

                                                                                               cgacgCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTgaa                                                       <  2:2722315/150‑1 (MQ=255)

                                                                                                  cgCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTgaagaa                                                    <  1:1187245/150‑1 (MQ=255)

                                                                                                   gCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGTAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAAc                                                   >  1:3787351/1‑150 (MQ=255)

                                                                                                     tGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCt                                                 >  2:576963/1‑150 (MQ=255)

                                                                                                     tGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCt                                                 <  1:90945/150‑1 (MQ=255)

                                                                                                       ccGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGt                                               <  2:5175826/150‑1 (MQ=255)

                                                                                                        cGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTc                                              <  2:1054894/150‑1 (MQ=255)

                                                                                                         ggTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTcc                                             <  2:4506387/150‑1 (MQ=255)

                                                                                                              cGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGcc                                        <  1:2464401/150‑1 (MQ=255)

                                                                                                                  accaccaccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATgc                                    <  2:3786/150‑1 (MQ=255)

                                                                                                                  accaccaccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATgc                                    >  2:722736/1‑150 (MQ=255)

                                                                                                                    caccaccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGc                                         <  1:4111439/143‑1 (MQ=255)

                                                                                                                      ccaccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCg                                >  1:4818386/1‑150 (MQ=255)

                                                                                                                       caccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGa                               <  2:3648861/150‑1 (MQ=255)

                                                                                                                         ccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACt                             >  1:146281/1‑150 (MQ=255)

                                                                                                                          cGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTc                            >  2:2894810/1‑150 (MQ=255)

                                                                                                                               ccGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAgg                       >  2:1967198/1‑150 (MQ=255)

                                                                                                                                 gTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGcc                     >  2:1265153/1‑150 (MQ=255)

                                                                                                                                       gC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGcc               <  1:4121359/150‑1 (MQ=255)

                                                                                                                                       gC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGcc               <  1:1928988/150‑1 (MQ=255)

                                                                                                                                        c‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGccc              <  2:918822/150‑1 (MQ=255)

                                                                                                                                              ggCCTTCGTTGACGATGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGaa                                                                                                    >  2:399129/1‑60 (MQ=25)

                                                                                                                                                  aTCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggtaggt      <  1:2340766/150‑1 (MQ=255)

                                                                                                                                                  aTCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggtaggt      <  1:1827329/150‑1 (MQ=255)

                                                                                                                                                      tCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGTCAAGGCCAGTGCTGCGGTCGTTGCCGAACTGAAGAACCTGTGGAAGCCATGCGCCGACTCGAAGGCCATCGCCCtggtaggttcgt  >  2:5329524/1‑146 (MQ=255)

AAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGTACCA  >  NC_002947/1554742‑1555039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`