Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,554,897 A→T 5.1% E102D (GAA→GAT)  groL → chaperonin GroEL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,554,8970AT 5.1% 868.7 / 21.7 255E102D (GAA→GAT) groLchaperonin GroEL
Reads supporting (aligned to +/- strand):  ref base A (111/131);  new base T (3/10);  total (114/141)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 261 total aligned reads displayed.

GGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGTACCATCTCTG  >  NC_002947/1554748‑1555045
                                                                                                                                                       |                                                                                                                                                    
ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGaa                                                                                                                                                      >  1:3085000/1‑150 (MQ=255)
ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCAGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGaa                                                                                                                                                      <  2:3724321/150‑1 (MQ=255)
    tttCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCTTCGTTGACGATGGcc                                                                                                                                                  <  1:1408035/150‑1 (MQ=255)
     ttCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCt                                                                                                                                                 <  1:4381499/150‑1 (MQ=255)
        cGTCGCCACAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGaa                                                                                                                                              <  2:1791875/150‑1 (MQ=255)
         gTCGCCAAAGAAATCGAGCTGAATGACGCTTTCGAAAACAGGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCACCGACGCTGCCGGTGACAGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGaaa                                                                                                                                             <  2:5176079/150‑1 (MQ=255)
           cGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTTTCAGGCCTTCGTTGACGATGGCCTGagc                                                                                                                                             <  1:3219704/150‑3 (MQ=255)
           cGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGc                                                                                                                                           <  1:3275960/150‑1 (MQ=255)
             ccAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCg                                                                                                                                         >  2:1928988/1‑150 (MQ=255)
              cAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGt                                                                                                                                        <  2:1959696/150‑1 (MQ=255)
               aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                                                                                                       >  1:3453394/1‑150 (MQ=255)
                      tCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCg                                                                                                                                <  1:4198123/150‑1 (MQ=255)
                          gCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCAt                                                                                                                            <  1:751751/150‑1 (MQ=255)
                          gCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCAt                                                                                                                            >  1:4269923/1‑150 (MQ=255)
                               aaGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAAcc                                                                                                                       <  2:412458/150‑1 (MQ=255)
                                 gACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCg                                                                                                                     >  2:1397381/1‑150 (MQ=255)
                                  aCGCTTTCGAAACCATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGACACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGa                                                                                                                    <  1:289221/150‑1 (MQ=255)
                                   cGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGAt                                                                                                                   <  2:5174262/150‑1 (MQ=255)
                                    gCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATg                                                                                                                  <  1:5113851/150‑1 (MQ=255)
                                      tttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGa                                                                                                                >  2:2193312/1‑150 (MQ=255)
                                      tttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGa                                                                                                                >  1:3528163/1‑150 (MQ=255)
                                      tttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGa                                                                                                                >  2:5023389/1‑150 (MQ=255)
                                       ttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGcc                                                                                                                                          <  1:2807498/123‑1 (MQ=255)
                                       ttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGAc                                                                                                               <  2:614521/150‑1 (MQ=255)
                                         cGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCt                                                                                                             <  2:2899333/150‑1 (MQ=255)
                                          gAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTg                                                                                                            <  1:84116/150‑1 (MQ=255)
                                          gAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTg                                                                                                            >  1:1524913/1‑150 (MQ=255)
                                               cATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcg                                                                                                       >  1:3047173/1‑150 (MQ=255)
                                               cATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcg                                                                                                       <  1:1934097/150‑1 (MQ=255)
                                                aTGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcgc                                                                                                      <  1:4885497/150‑1 (MQ=255)
                                                aTGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcgc                                                                                                      >  2:1832720/1‑150 (MQ=255)
                                                aTGGGCGCCCAGCGGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCGACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcgc                                                                                                      <  2:3577657/150‑1 (MQ=255)
                                                 tGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                                                                                                     <  2:2033970/150‑1 (MQ=255)
                                                  gggCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCgg                                                                                                    >  1:1637016/1‑150 (MQ=255)
                                                    gcgcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTa                                                                                                  >  1:104851/1‑150 (MQ=255)
                                                     cgcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTAt                                                                                                 <  2:338630/150‑1 (MQ=255)
                                                     cgcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTAt                                                                                                 <  1:1689732/150‑1 (MQ=255)
                                                      gcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATc                                                                                                <  1:4926649/150‑1 (MQ=255)
                                                          aGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACa                                                                                            >  1:5257051/1‑150 (MQ=255)
                                                           gCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACaa                                                                                           <  1:4620695/150‑1 (MQ=255)
                                                               gTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAg                                                                                                         <  1:1266597/132‑1 (MQ=255)
                                                               gTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAg                                                                                                         >  2:1266597/1‑132 (MQ=255)
                                                                tCAAGGAAGTTGCTTCCAAGGCCAACGCCGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCa                                                                                      <  2:4645361/150‑1 (MQ=255)
                                                                  aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct                                                                                    >  1:3400163/1‑150 (MQ=255)
                                                                  aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct                                                                                    >  2:2780570/1‑150 (MQ=255)
                                                                        gTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGcc                                                                              <  1:5595798/150‑1 (MQ=255)
                                                                        gTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGcc                                                                              <  2:5238265/150‑1 (MQ=255)
                                                                            cTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGt                                                                                                   >  2:4452287/1‑125 (MQ=255)
                                                                            cTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGt                                                                                                   <  1:4452287/125‑1 (MQ=255)
                                                                             ttCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcgtcgt                                                                         >  1:5298503/1‑150 (MQ=255)
                                                                             ttCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcgtcgt                                                                         >  1:2412259/1‑150 (MQ=255)
                                                                              tCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGtt                                                                        >  1:1810686/1‑150 (MQ=255)
                                                                               ccAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTg                                                                       >  2:4408286/1‑150 (MQ=255)
                                                                               ccAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTg                                                                       >  2:2620014/1‑150 (MQ=255)
                                                                                cAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGc                                                                      >  2:1314441/1‑150 (MQ=255)
                                                                                 aaGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGcc                                                                     >  2:775430/1‑150 (MQ=255)
                                                                                  aGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCg                                                                    <  1:4023215/150‑1 (MQ=255)
                                                                                    gCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGaa                                                                  <  1:5432089/150‑1 (MQ=255)
                                                                                     ccAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAAc                                                                 <  2:2920205/150‑1 (MQ=255)
                                                                                      cAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACt                                                                >  2:4272912/1‑150 (MQ=255)
                                                                                       aaCGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTg                                                               >  1:68601/1‑150 (MQ=255)
                                                                                        acgacgCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTga                                                              <  1:2972954/150‑1 (MQ=255)
                                                                                            cgCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTgaagaa                                                          <  1:1187245/150‑1 (MQ=255)
                                                                                             gCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGTAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAAc                                                         >  1:3787351/1‑150 (MQ=255)
                                                                                             gCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAAc                                                         <  2:223992/150‑1 (MQ=255)
                                                                                             gCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAAc                                                         <  2:5359611/150‑1 (MQ=255)
                                                                                              cTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAAcc                                                        <  2:574023/150‑1 (MQ=255)
                                                                                               tGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCt                                                       <  1:90945/150‑1 (MQ=255)
                                                                                               tGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCt                                                       >  2:4855626/1‑150 (MQ=255)
                                                                                                  cGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTc                                                    >  2:4601395/1‑150 (MQ=255)
                                                                                                  cGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGGATCGACAAGGCCACGGCTGCCGTCGTTGCCGAACTGAAGAACCTGTc                                                    >  1:1336981/1‑150 (MQ=255)
                                                                                                        cGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGcc                                              <  1:2464401/150‑1 (MQ=255)
                                                                                                        cGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTCCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGcc                                              <  2:1020461/150‑1 (MQ=255)
                                                                                                         ggCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCa                                             >  2:1469633/1‑150 (MQ=255)
                                                                                                            accaccaccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATgc                                          <  2:3786/150‑1 (MQ=255)
                                                                                                            accaccaccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATgc                                          >  2:722736/1‑150 (MQ=255)
                                                                                                              caccaccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGc                                               <  1:4111439/143‑1 (MQ=255)
                                                                                                              caccaccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGc                                               >  2:4111439/1‑143 (MQ=255)
                                                                                                               accaccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGcc                                       <  2:2749994/150‑1 (MQ=255)
                                                                                                                ccaccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCAGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGAATCGACAAGGCCACTGCTGCCGTCGTAGCCGAACTGAAGAACCTGTCCAAGCCATGCGACg                                      >  1:4818473/1‑150 (MQ=255)
                                                                                                                 caccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGa                                     <  2:3648861/150‑1 (MQ=255)
                                                                                                                 caccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGa                                     <  1:4498584/150‑1 (MQ=255)
                                                                                                                   ccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACt                                   >  1:146281/1‑150 (MQ=255)
                                                                                                                    cGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTc                                  >  2:4044780/1‑150 (MQ=255)
                                                                                                                    cGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTc                                  >  2:2894810/1‑150 (MQ=255)
                                                                                                                        aCCGTTCTGGC‑‑CCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACCGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAg                              <  2:118605/150‑1 (MQ=255)
                                                                                                                           gTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGcc                           >  2:4774031/1‑150 (MQ=255)
                                                                                                                                ggctttCAGGCCTTCGTTGACGATGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATc                        >  2:5543276/6‑150 (MQ=255)
                                                                                                                                 gC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGcc                     <  1:4121359/150‑1 (MQ=255)
                                                                                                                                 gC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGcc                     <  1:1928988/150‑1 (MQ=255)
                                                                                                                                  c‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGccc                    <  2:918822/150‑1 (MQ=255)
                                                                                                                                     tCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCa                   <  2:3528163/150‑1 (MQ=255)
                                                                                                                                     tCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCa                   >  2:3252652/1‑150 (MQ=255)
                                                                                                                                     tCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCa                   >  2:586207/1‑150 (MQ=255)
                                                                                                                                     tCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCa                   <  1:216755/150‑1 (MQ=255)
                                                                                                                                     tCAGGACATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCa                   <  2:3165021/150‑1 (MQ=255)
                                                                                                                                         gCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggta               >  1:2516434/1‑150 (MQ=255)
                                                                                                                                            aTCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggtaggt            <  1:2340766/150‑1 (MQ=255)
                                                                                                                                                    cGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGTACCAtctc    >  2:172962/1‑150 (MQ=255)
                                                                                                                                                     gAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGTACCATctct   <  2:3062651/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
GGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGTACCATCTCTG  >  NC_002947/1554748‑1555045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑