BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,580,189	G→A	8.2%	intergenic (‑184/+26)	pcaP ← / ← galP‑II	porin/porin‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,580,189	0	G	A	8.2%	802.7 / 41.3	266	intergenic (‑184/+26)	pcaP/galP‑II	porin/porin‑like protein
Reads supporting (aligned to +/- strand): ref base G (128/116); new base A (11/11); total (139/127)
Fisher's exact test for biased strand distribution p-value = 8.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

CCTGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGC  >  NC_002947/1580042‑1580338

ccTGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGtt                                                                                                                                                     <  1:2989954/150‑1 (MQ=255)

 cTGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGttt                                                                                                                                                    >  2:4488348/1‑150 (MQ=255)

  tGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTa                                                                                                                                                   >  1:4247010/1‑150 (MQ=255)

  tGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTa                                                                                                                                                   >  2:2088124/1‑150 (MQ=255)

   gATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTAt                                                                                                                                                  >  1:1077996/1‑150 (MQ=255)

   gATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTAt                                                                                                                                                  >  1:3905804/1‑150 (MQ=255)

   gATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTAt                                                                                                                                                  >  2:4690084/1‑150 (MQ=255)

        ataTCAGGATAGCCTGCAGTCACCACCATCATCAGCTCATAGAATGTCGTCAGCTCGCACTTGTCAATTAATGGCAGGGTTTTCATCACGCTGTTGCCAAAGATGCCTTCAAAAGGGAGGGAGTCTGGCCTATTCGCGGGTTTATCcgag                                                                                                                                             >  2:2599353/3‑148 (MQ=255)

         gATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGa                                                                                                                                            <  2:2641538/150‑1 (MQ=255)

         gATCAGGAGAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGa                                                                                                                                            <  2:3369061/150‑1 (MQ=255)

          aTCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGaa                                                                                                                                           <  1:4551258/150‑1 (MQ=255)

            cAGGATAGCCTGCAGTCACCACCATCATCAGCTCGGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGATAAACCCGCGaata                                                                                                                                         <  1:1923280/150‑3 (MQ=255)

            cAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAaga                                                                                                                                         >  2:752410/1‑150 (MQ=255)

              ggATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAATAgg                                                                                                                                       <  1:808079/150‑1 (MQ=255)

              ggATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAgg                                                                                                                                       <  1:5540086/150‑1 (MQ=255)

              ggATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAgg                                                                                                                                       >  1:2209357/1‑150 (MQ=255)

               gATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGc                                                                                                                                      <  2:1280579/150‑1 (MQ=255)

                aTAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAATAGGcc                                                                                                                                     <  1:2289469/150‑1 (MQ=255)

                   gCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAt                                                                                                                                  >  2:3301052/1‑150 (MQ=255)

                    ccTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                                 <  1:1168688/150‑1 (MQ=255)

                    ccTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                                 <  1:5241293/150‑1 (MQ=255)

                     cTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTg                                                                                                                                >  2:2716072/1‑150 (MQ=255)

                      tGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGa                                                                                                                               >  2:1879299/1‑150 (MQ=255)

                      tGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGa                                                                                                                               <  1:1009813/150‑1 (MQ=255)

                        cAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGt                                                                                                                             <  1:2577439/150‑1 (MQ=255)

                            caccacCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTcaca                                                                                                                         <  2:2865490/150‑1 (MQ=255)

                             accaccATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACag                                                                                                                        >  1:3013658/1‑150 (MQ=255)

                               caccaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACagaa                                                                                                                      <  1:1635662/150‑1 (MQ=255)

                                accaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                                 >  2:2939016/1‑138 (MQ=255)

                                accaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                                 <  1:2939016/138‑1 (MQ=255)

                                accaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACagaag                                                                                                                     >  2:2184321/1‑150 (MQ=255)

                                accaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACagaag                                                                                                                     >  1:2340162/1‑150 (MQ=255)

                                 ccaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACagaaga                                                                                                                    >  1:1431534/1‑150 (MQ=255)

                                    tcatcaGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTc                                                                                                                 >  2:1022756/1‑150 (MQ=255)

                                      atcaGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                               <  1:2075743/150‑1 (MQ=255)

                                      atcaGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                               <  1:2425715/150‑1 (MQ=255)

                                      atcaGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                               <  2:4561117/150‑1 (MQ=255)

                                       tcagctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGc                                                                                                              >  2:2354164/1‑150 (MQ=255)

                                            tcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAatag                                                                                                                                        >  1:5183550/1‑119 (MQ=38)

                                            tcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAatag                                                                                                                                        <  2:5183550/119‑1 (MQ=38)

                                                cGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTc                                                                                                     >  1:3751788/1‑150 (MQ=255)

                                                 gAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTcac                                                                                                                          <  2:4984641/128‑1 (MQ=255)

                                                 gAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTcac                                                                                                                          >  1:4984542/1‑128 (MQ=255)

                                                   aTGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAATAGGCCagac                                                                                                                                 >  1:289565/1‑116 (MQ=37)

                                                       cggcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCa                                                                                              >  1:30435/1‑150 (MQ=255)

                                                              tCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGtt                                                                                       >  2:912159/1‑150 (MQ=255)

                                                                gCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATaa                                                                                                        <  2:2287335/131‑1 (MQ=255)

                                                                 cACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTtctc                                                                                    <  2:1979952/150‑1 (MQ=255)

                                                                    tGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATc                                                                                 <  1:3439748/150‑1 (MQ=255)

                                                                        cAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAAc                                                                             <  2:1590975/150‑1 (MQ=255)

                                                                            tCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGt                                                                         >  1:3788376/1‑150 (MQ=255)

                                                                             cATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGtt                                                                        <  1:5595167/150‑1 (MQ=255)

                                                                                ggCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAatag                                                                                                                                        >  2:2835817/1‑83 (MQ=25)

                                                                                   aGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCt                                                                  >  2:1725936/1‑150 (MQ=255)

                                                                                   aGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGACTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAGTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGATGGTGCt                                                                  >  1:461177/1‑150 (MQ=255)

                                                                                    gggTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                                 <  1:4374070/86‑1 (MQ=255)

                                                                                      gTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCACGCGGTTCTCATCGAACGCGTTGGTGCTGaa                                                               <  2:4043377/150‑1 (MQ=255)

                                                                                             tcaCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGa                                                        >  2:1823966/1‑150 (MQ=255)

                                                                                             tcaCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGa                                                        <  2:2138458/150‑1 (MQ=255)

                                                                                              caCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGAc                                                       <  2:3898273/150‑1 (MQ=255)

                                                                                               aCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACg                                                      >  2:4296062/1‑150 (MQ=255)

                                                                                                   tGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATg                                                  <  2:4966302/150‑1 (MQ=255)

                                                                                                        ccAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGa                                             >  2:4875595/1‑150 (MQ=255)

                                                                                                        ccAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGa                                             >  2:4168992/1‑150 (MQ=255)

                                                                                                        ccAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGa                                             <  1:4140334/150‑1 (MQ=255)

                                                                                                         cAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAg                                            >  2:356415/1‑150 (MQ=255)

                                                                                                           atgatgCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGtt                                          >  1:586820/1‑150 (MQ=255)

                                                                                                             gatgCCTTCAACAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgc                                        <  2:5361010/150‑1 (MQ=255)

                                                                                                             gatgCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgc                                        <  1:3275138/150‑1 (MQ=255)

                                                                                                             gatgCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTCgc                                        <  1:773599/150‑1 (MQ=255)

                                                                                                              atgCCTTCAAACGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGACCGCGTTGGTGCTGAAGTCGCGACGCAGGGTCGAGTTgcg                                       <  2:3032346/150‑1 (MQ=255)

                                                                                                               tgCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgcgc                                      <  1:2839282/150‑1 (MQ=255)

                                                                                                                  cTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAt                                   >  1:5114143/1‑150 (MQ=255)

                                                                                                                  cTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAt                                   <  1:389666/150‑1 (MQ=255)

                                                                                                                   ttCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAtt                                  >  1:2615442/1‑150 (MQ=255)

                                                                                                                    tCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAttt                                 <  2:1311336/150‑1 (MQ=255)

                                                                                                                      aaaaGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCa                               <  2:1077996/150‑1 (MQ=255)

                                                                                                                       aaaGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAg                              <  2:1431534/150‑1 (MQ=255)

                                                                                                                        aaGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGc                             <  1:2571598/150‑1 (MQ=255)

                                                                                                                              actcaattGGCCTCTTCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAAATCACAATCAAGCGGTTCTCATCGAAAGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGAGCCATTTCAGCGACAgg                       >  2:570301/8‑150 (MQ=37)

                                                                                                                                gCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACATAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTc                     <  1:1575630/150‑1 (MQ=255)

                                                                                                                                 cGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTcc                    <  2:516952/150‑1 (MQ=255)

                                                                                                                                 cGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTcc                    <  1:2716072/150‑1 (MQ=255)

                                                                                                                                  gtatGGCCTATTCTAGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATAAATCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCt                   <  1:3141280/147‑1 (MQ=255)

                                                                                                                                  aattGGCCTCTTCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCt                   >  2:1969370/4‑150 (MQ=39)

                                                                                                                                     tGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGa                >  2:5085111/1‑150 (MQ=255)

                                                                                                                                     tGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGa                >  2:644341/1‑150 (MQ=255)

                                                                                                                                     tGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGa                >  1:1249371/1‑150 (MQ=255)

                                                                                                                                      ggCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGaa               <  1:260570/150‑1 (MQ=255)

                                                                                                                                       gCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAg              >  1:4365753/1‑150 (MQ=255)

                                                                                                                                         ctcttCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCt            >  1:4433112/4‑150 (MQ=255)

                                                                                                                                         cTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCt            >  1:3430145/1‑150 (MQ=255)

                                                                                                                                          tATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTg           >  1:2478585/1‑150 (MQ=255)

                                                                                                                                             tCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGccc        >  1:475729/1‑150 (MQ=255)

                                                                                                                                               gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTcg                                                           >  2:3818123/1‑97 (MQ=255)

                                                                                                                                               gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTcg                                                           <  1:3818123/97‑1 (MQ=255)

                                                                                                                                               gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGa      >  2:3855468/1‑150 (MQ=255)

                                                                                                                                               gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGa      >  1:4933584/1‑150 (MQ=255)

                                                                                                                                                   gTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGc  >  1:1464962/1‑150 (MQ=255)

                                                                                                                                                   gTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGc  >  1:500616/1‑150 (MQ=255)

CCTGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGC  >  NC_002947/1580042‑1580338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`