Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,603,819 A→G 7.4% intergenic (‑67/+64) dctB ← / ← bglX C4‑dicarboxylate transport sensor protein/beta‑D‑glucoside glucohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,603,8190AG 7.4% 629.4 / 24.7 202intergenic (‑67/+64)dctB/bglXC4‑dicarboxylate transport sensor protein/beta‑D‑glucoside glucohydrolase
Reads supporting (aligned to +/- strand):  ref base A (82/105);  new base G (5/10);  total (87/115)
Fisher's exact test for biased strand distribution p-value = 5.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

CCAGCCGGCGCTGAGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCCGCT  >  NC_002947/1603672‑1603968
                                                                                                                                                   |                                                                                                                                                     
ccAGCCGGCGCTGAGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTAct                                                                                                                                                     >  1:2460508/1‑150 (MQ=255)
 cAGCCGGCGCTGAGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTActc                                                                                                                                                    >  2:3038506/1‑150 (MQ=255)
     cGGCGCTGAGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTgca                                                                                                                                                <  2:1849623/150‑1 (MQ=255)
       gcgcTGAGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTGctgcagagt                                                                                                                                              <  1:5084516/150‑8 (MQ=255)
           tGAGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCt                                                                                                                                          >  1:3479517/1‑150 (MQ=255)
           tGAGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCt                                                                                                                                          <  1:3396197/150‑1 (MQ=255)
           cgAGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCt                                                                                                                                          <  2:546924/149‑1 (MQ=255)
             aGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGt                                                                                                                                        <  1:3790881/150‑1 (MQ=255)
             aGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGt                                                                                                                                        <  1:2905603/150‑1 (MQ=255)
              gTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTa                                                                                                                                       <  1:320300/150‑1 (MQ=255)
               tGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTGCTGCAGAGTACCTGTAg                                                                                                                                      <  1:5432495/150‑1 (MQ=38)
               tGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAg                                                                                                                                      <  1:1360611/150‑1 (MQ=255)
               tGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAg                                                                                                                                      <  2:4551666/150‑1 (MQ=255)
                gCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAAACCGCGCCTACAGGTGCTGCAGAGTACCTGTAgg                                                                                                                                     <  2:3161472/150‑1 (MQ=38)
                 cGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGgc                                                                                                                                    <  1:3114833/150‑1 (MQ=255)
                 cGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGgc                                                                                                                                    <  1:1441888/150‑1 (MQ=255)
                   gTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGgcgc                                                                                                                                  <  1:5532791/150‑1 (MQ=255)
                   gTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGgcgc                                                                                                                                  <  2:3244377/150‑1 (MQ=255)
                    tACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGcgcg                                                                                                                                 <  1:1794280/150‑1 (MQ=255)
                      cccAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGt                                                                                                                               <  2:5508132/150‑1 (MQ=255)
                       ccAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGtt                                                                                                                              >  2:4092191/1‑150 (MQ=255)
                       ccAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGtt                                                                                                                              <  1:5283142/150‑1 (MQ=255)
                          gCACGATCAGCTGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAAACCGCGCCTACAGGTGCTGCAGAGTACGTGTAGGCGCGGGTTTa                                                                                                                           <  1:4174237/150‑1 (MQ=25)
                          gCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCGGCCTTTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTa                                                                                                                           <  1:453570/150‑1 (MQ=255)
                           cACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTAc                                                                                                                          <  2:3469485/150‑1 (MQ=255)
                           cACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAAACCGCGCCTACAGGTGCTGCAGAGTACCTGTAGGCGCGGGTTTAc                                                                                                                          <  2:2604115/150‑1 (MQ=25)
                           cACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAAACCGCGCCTACAGGTGCTGCAGAGTACCTGTAGGCGCGGGTTTAc                                                                                                                          <  2:554815/150‑1 (MQ=25)
                            aCGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTAcc                                                                                                                         >  2:5081100/1‑150 (MQ=255)
                               aTCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCcgc                                                                                                                      <  2:1017118/150‑1 (MQ=255)
                                   gcagGGTGATCAGCCCCAGACGCCGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTGGTACCCGCGaca                                                                                                                  >  2:4973624/1‑148 (MQ=255)
                                    cagGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGaaaa                                                                                                                 <  2:2139949/150‑1 (MQ=255)
                                     agGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAg                                                                                                                <  1:3036703/150‑1 (MQ=255)
                                     agGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAg                                                                                                                >  2:4643242/1‑150 (MQ=255)
                                       ggTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGc                                                                                                              <  2:726166/150‑1 (MQ=255)
                                        gTGATCAGCCCCCGACGCAGGGCGCTAAAGGAAATGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCACTTCGCGGGTAAACCCGCGCCTCCAGGTACTCTGCAGCACCTGTAGGCGCGGGTTTACCCGCGAAGAGGca                                                                                                             <  2:3828165/150‑2 (MQ=38)
                                        gTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGcc                                                                                                             <  2:138320/150‑1 (MQ=255)
                                        gTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGcc                                                                                                             <  1:562160/150‑1 (MQ=255)
                                         tGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCg                                                                                                            <  1:5061763/150‑1 (MQ=255)
                                              aGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAGGGCCGGAACa                                                                                                       >  2:4391187/1‑150 (MQ=255)
                                               gCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAg                                                                                                      >  2:5027278/1‑150 (MQ=255)
                                                ccccAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAgg                                                                                                     <  1:2797594/150‑1 (MQ=255)
                                                  ccAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAggcg                                                                                                   >  2:3010319/1‑150 (MQ=255)
                                                  ccAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCGACAGGTACTCTGCAGCACCAGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAggcg                                                                                                   >  1:2882927/1‑150 (MQ=255)
                                                   cAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTActct                                                                                                                                                   <  1:3739752/101‑1 (MQ=255)
                                                   cAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTActct                                                                                                                                                   >  2:3739752/1‑101 (MQ=255)
                                                    aGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAggcggc                                                                                                 <  2:1917573/150‑1 (MQ=255)
                                                         cAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCtttt                                                                                            <  1:1193657/150‑1 (MQ=255)
                                                         cAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCtttt                                                                                            <  1:5388895/150‑1 (MQ=255)
                                                           gggCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTc                                                                                          <  1:399427/150‑1 (MQ=255)
                                                            ggCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCa                                                                                         <  1:2374289/150‑1 (MQ=255)
                                                            ggCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGCTACTCTGCAGCACCTGTAGGCGCGGTATTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCa                                                                                         <  2:4057900/150‑1 (MQ=255)
                                                                  aaaGGAAAATGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCATGCCTCTTCGCGGGTAAAACCGCGCCTACAGGTACACTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAATGCCGGAACAGGCGGCCTTTTTCAGTCaaa                                                                                   >  2:5280540/1‑150 (MQ=39)
                                                                   aaGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAg                                                                                  >  2:5389605/1‑150 (MQ=255)
                                                                      gAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCaa                                                                               >  2:2188777/1‑150 (MQ=255)
                                                                       aaaaGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAAt                                                                              >  1:4787199/1‑150 (MQ=255)
                                                                         aaGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTc                                                                            <  2:5119686/150‑1 (MQ=255)
                                                                               aTCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCttt                                                                                             >  2:3263482/1‑127 (MQ=255)
                                                                               aTCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCGGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGct                                                                      >  1:3312455/1‑150 (MQ=255)
                                                                               aTCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAAACCGCGCCTACAGGTGCTGCAGAGTACCTGTAGGCGCgggt                                                                                                                              <  2:2124784/94‑3 (MQ=17)
                                                                                tCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGctc                                                                     >  1:4590046/1‑150 (MQ=255)
                                                                                   ggggCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTctgc                                                                  >  1:2754849/1‑150 (MQ=255)
                                                                                    gggCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTctgct                                                                 >  1:2117542/1‑150 (MQ=255)
                                                                                              ggtggtGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGc                                                       >  1:5004826/1‑150 (MQ=255)
                                                                                                 ggtggGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTg                                                    <  1:3655416/150‑1 (MQ=255)
                                                                                                  gtggGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTgg                                                   <  1:198100/150‑1 (MQ=255)
                                                                                                    gggATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGa                                                 >  2:3077906/1‑150 (MQ=255)
                                                                                                       aTGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCAACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAGTc                                              >  1:715196/1‑150 (MQ=255)
                                                                                                         gTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCcgcg                                                                                                                     >  1:875992/1‑77 (MQ=255)
                                                                                                         gTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTATTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCa                                            <  1:3596272/150‑1 (MQ=255)
                                                                                                            ggggCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGcc                                         >  1:1295337/1‑150 (MQ=255)
                                                                                                              ggCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCt                                       >  2:3959601/1‑150 (MQ=255)
                                                                                                                cctgcctCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTAc                                     <  2:3588860/150‑1 (MQ=255)
                                                                                                                 ctgcctCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTAcc                                    >  1:4919223/1‑150 (MQ=255)
                                                                                                                  tgcctCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCt                                   >  2:2290447/1‑150 (MQ=255)
                                                                                                                  tgcctCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCt                                   <  1:1426198/150‑1 (MQ=255)
                                                                                                                     ctctTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCAGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCAGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGa                                >  2:808407/1‑150 (MQ=255)
                                                                                                                      tctTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCCGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTAAAATCACAGCTGCTGCACCGCCGGGGAATCCAGCCCTACCTGGAc                               >  1:4250123/1‑150 (MQ=38)
                                                                                                                         tCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACAtt                            <  1:3731186/150‑1 (MQ=255)
                                                                                                                            cgGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGaa                         <  1:4434316/150‑1 (MQ=255)
                                                                                                                            cgGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGaa                         <  2:1295337/150‑1 (MQ=255)
                                                                                                                            cgGGTAAACCCGCGCCTACAGGTACGCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGaa                         <  1:5257311/150‑1 (MQ=255)
                                                                                                                             gggTAAACCCGCGCCTACAGGTACTCTGCCGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGACCAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCCCCGCCTGGGAATCCAGCCCTACCTGGACCGTGAAc                        >  2:1057665/1‑150 (MQ=255)
                                                                                                                             gggTAAAACCGCGCCTACAGGTGCTGCAGAGTACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAAc                        >  2:3538618/1‑150 (MQ=37)
                                                                                                                              ggTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACt                       >  2:3448090/1‑150 (MQ=255)
                                                                                                                               gTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTc                      <  1:860417/150‑1 (MQ=255)
                                                                                                                               gTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTc                      <  1:1625231/150‑1 (MQ=255)
                                                                                                                                 aaaaCCGCGCCTACAGGTGCTGCAGAGTACCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTccc                    >  2:4922066/1‑150 (MQ=25)
                                                                                                                                 aaaCCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTccc                    <  1:5369585/150‑1 (MQ=255)
                                                                                                                                  aaCCCGCGCCTACCGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTcccc                   <  2:2460508/150‑1 (MQ=255)
                                                                                                                                      cgcgCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGTAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGt               <  1:2406520/150‑1 (MQ=255)
                                                                                                                                         gcCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCg            <  1:5503738/150‑1 (MQ=255)
                                                                                                                                         gcCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCATTTACAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCg            <  2:4185233/150‑1 (MQ=255)
                                                                                                                                           cTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGc          >  1:4886773/1‑150 (MQ=255)
                                                                                                                                            tACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACCTTGAACTCCCCTGGTTCGGCa         >  1:395752/1‑150 (MQ=255)
                                                                                                                                            tACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCa         >  2:4900509/1‑150 (MQ=255)
                                                                                                                                             aCAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCaa        >  2:1959567/1‑150 (MQ=255)
                                                                                                                                             aCAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCaa        <  1:3038506/150‑1 (MQ=255)
                                                                                                                                                 gTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCCg    >  2:3483136/1‑150 (MQ=255)
                                                                                                                                                  tACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCCGc   >  2:2371411/1‑150 (MQ=255)
                                                                                                                                                   aCTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCCGCt  <  1:4077228/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCAGCCGGCGCTGAGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCCGCT  >  NC_002947/1603672‑1603968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑