BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,144,629	C→A	5.4%	Q162H (CAG→CAT)	PP_5481 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,144,629	0	C	A	5.4%	821.7 / 24.3	241	Q162H (CAG→CAT)	PP_5481	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (108/120); new base A (8/5); total (116/125)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

TTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAGCTCGTCGGG  >  NC_002947/2144480‑2144778

ttCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCAc                                                                                                                                                       <  2:5489363/150‑1 (MQ=255)

   tAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACtac                                                                                                                                                    >  2:4903673/1‑148 (MQ=255)

   tAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGc                                                                                                                                                    <  1:1218313/150‑1 (MQ=255)

     ggggTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCg                                                                                                                                                  <  2:4494944/150‑1 (MQ=255)

     ggggTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCg                                                                                                                                                  <  1:1632599/150‑1 (MQ=255)

      gggTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCgg                                                                                                                                                 >  2:5182456/1‑150 (MQ=255)

       ggTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGc                                                                                                                                                <  1:3368504/150‑1 (MQ=255)

        gtgtCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCGCCGGCAGTGGCGTCGTCGCCAATGCCggtg                                                                                                                                               <  1:4289820/150‑3 (MQ=255)

         tgtCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGccc                                                                                                                                              >  1:1032839/1‑150 (MQ=255)

         tgtCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGccc                                                                                                                                              >  1:3861490/1‑150 (MQ=255)

             ggATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGcccaccc                                                                                                                                          >  1:4683376/1‑150 (MQ=255)

             ggATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGcccaccc                                                                                                                                          >  2:2124585/1‑150 (MQ=255)

             ggATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGcccaccc                                                                                                                                          >  2:2247544/1‑150 (MQ=255)

               atatCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCccacccac                                                                                                                                        <  2:3319177/150‑1 (MQ=255)

               atatCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCccacccac                                                                                                                                        <  2:5608326/150‑1 (MQ=255)

                  tCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAg                                                                                                                                     >  2:1414492/1‑150 (MQ=255)

                   cAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAgc                                                                                                                                    >  2:675394/1‑150 (MQ=255)

                   cAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAgc                                                                                                                                    <  2:5280804/150‑1 (MQ=255)

                     gCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAgcgc                                                                                                                                  <  2:1185911/150‑1 (MQ=255)

                        ttGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAg                                                                                                                               <  1:4884870/150‑1 (MQ=255)

                           gCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAg                                                                                                                            >  2:1543251/1‑150 (MQ=255)

                             aCGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGaa                                                                                                                          <  2:2569809/150‑1 (MQ=255)

                             aCGGAGATCACCTTCATGCAGGTGGCCACTGCGGTGCTCTACGTAGTTTGCGTTCGCTTGCTGGTCAGAGGCCGCGGCATGCGGGCCTGAAGCCTTCCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAgaagaa                                                                                                                          >  2:4656071/1‑150 (MQ=255)

                                    tCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCGTTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAtggtg                                                                                                                   <  1:1703965/150‑1 (MQ=255)

                                    tCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAtggtg                                                                                                                   <  2:3901718/150‑1 (MQ=255)

                                      aCCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGga                                                                                                                 >  2:2442434/1‑150 (MQ=255)

                                      aCCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGga                                                                                                                 <  2:799685/150‑1 (MQ=255)

                                        cTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGgaga                                                                                                               >  2:3495419/1‑150 (MQ=255)

                                           cATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGt                                                                                                            >  2:4562477/1‑150 (MQ=255)

                                             tGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCCGCGCCAGTAGACCATGGTGGAGAAGTAg                                                                                                          >  1:2163428/1‑150 (MQ=255)

                                             tGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAg                                                                                                          <  2:4596205/150‑1 (MQ=255)

                                             tGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAg                                                                                                          >  1:1145291/1‑150 (MQ=255)

                                                tggtggCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCa                                                                                                       >  2:2207435/1‑150 (MQ=255)

                                                 ggtggCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCaa                                                                                                      <  1:3093589/150‑1 (MQ=255)

                                                  gtggCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAg                                                                                                     <  1:4516245/150‑1 (MQ=255)

                                                  gtggCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAg                                                                                                     <  1:2031504/150‑1 (MQ=255)

                                                  gtggCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAg                                                                                                     >  2:4736174/1‑150 (MQ=255)

                                                       cACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGc                                                                                                >  1:1160703/1‑150 (MQ=255)

                                                       cACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGc                                                                                                >  1:625916/1‑150 (MQ=255)

                                                        aCTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGcc                                                                                               >  2:4204815/1‑150 (MQ=255)

                                                                gCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGcacca                                                                                       >  1:4437096/1‑150 (MQ=255)

                                                                  tctACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCAcg                                                                                     <  1:5011316/150‑1 (MQ=255)

                                                                    tACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCAcgcc                                                                                   >  2:5423728/1‑150 (MQ=255)

                                                                      cGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCAcgccgc                                                                                 <  1:3056810/150‑1 (MQ=255)

                                                                              gCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACaccacc                                                                         <  1:490839/150‑1 (MQ=255)

                                                                                gTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccga                                                                       <  1:2355615/150‑1 (MQ=255)

                                                                                    gCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagccg                                                                   >  1:1482364/1‑150 (MQ=255)

                                                                                    gCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagccg                                                                   >  1:1482125/1‑150 (MQ=255)

                                                                                      ttGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagccgag                                                                 >  1:3807093/1‑150 (MQ=255)

                                                                                        gCTGGTCAGCGGCCGCGGCATGCGGTCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCa                                                               <  1:1970094/150‑1 (MQ=255)

                                                                                        gCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCa                                                               <  1:5344609/150‑1 (MQ=255)

                                                                                          tGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAgg                                                             >  2:3265071/1‑150 (MQ=255)

                                                                                            gTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCTCACCACCGAGCCGAGCAGGaa                                                           >  2:4053251/1‑150 (MQ=255)

                                                                                             tCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAg                                                          <  1:3046676/150‑1 (MQ=255)

                                                                                             tCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAg                                                          >  2:3798320/1‑150 (MQ=255)

                                                                                              cAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAgg                                                         <  1:528090/150‑1 (MQ=255)

                                                                                              cAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAgg                                                         <  2:280899/150‑1 (MQ=255)

                                                                                                 cGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCa                                                               >  2:4454603/1‑141 (MQ=255)

                                                                                                 cGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGtt                                                      >  1:290707/1‑150 (MQ=255)

                                                                                                   gCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGttac                                                    <  2:1862914/150‑3 (MQ=255)

                                                                                                   gCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGc                                                    <  1:2479761/150‑1 (MQ=255)

                                                                                                   gCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGc                                                    <  1:5630383/150‑1 (MQ=255)

                                                                                                   gCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGc                                                    <  1:5423728/150‑1 (MQ=255)

                                                                                                   gCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGc                                                    <  2:643928/150‑1 (MQ=255)

                                                                                                       cgGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCcaca                                                <  2:2727070/150‑1 (MQ=255)

                                                                                                         gCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGc                                              <  1:1543251/150‑1 (MQ=255)

                                                                                                         gCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGc                                              <  1:4379423/150‑1 (MQ=255)

                                                                                                           aTGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCACACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGt                                            <  2:1063001/150‑1 (MQ=255)

                                                                                                             gCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCATCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCg                                          >  2:698287/1‑150 (MQ=255)

                                                                                                               gggCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGAc                                        >  1:521586/1‑150 (MQ=255)

                                                                                                               gggCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGAc                                        >  1:2698597/1‑150 (MQ=255)

                                                                                                                 gCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGTGCCGAGCa                                                               <  2:5055929/125‑1 (MQ=255)

                                                                                                                 gCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAg                                      >  2:2630527/1‑150 (MQ=255)

                                                                                                                  ccTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGc                                     >  1:1416129/1‑150 (MQ=255)

                                                                                                                   cTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCt                                    <  2:3463693/150‑1 (MQ=255)

                                                                                                                      aaGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGt                                 <  2:1022446/150‑1 (MQ=255)

                                                                                                                         gggtGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagccgag                                                                 >  1:3645606/4‑115 (MQ=255)

                                                                                                                         ccTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCgg                              <  2:2849457/150‑1 (MQ=255)

                                                                                                                          cTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGt                             <  2:3067113/150‑1 (MQ=255)

                                                                                                                              ggccGGCAGTGGCGTCGTCGCCAATGCCGGTCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATc                         >  2:2916435/3‑150 (MQ=255)

                                                                                                                              ggccGGCAGTGGCGTCGTCGCCAATGCCGGTCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATc                         >  1:726028/3‑150 (MQ=255)

                                                                                                                              cACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATc                         <  2:3011591/150‑1 (MQ=255)

                                                                                                                                 cGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCAc                      >  1:2374351/1‑150 (MQ=255)

                                                                                                                                 cGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCAc                      <  1:2797371/150‑1 (MQ=255)

                                                                                                                                 cGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCAc                      <  1:31644/150‑1 (MQ=255)

                                                                                                                                   gCAGTGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTg                    >  2:4387410/1‑150 (MQ=255)

                                                                                                                                      ttGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGaa                 <  1:1133803/150‑1 (MQ=255)

                                                                                                                                         gCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGt              <  1:902703/150‑1 (MQ=255)

                                                                                                                                         gCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGt              >  1:1204512/1‑150 (MQ=255)

                                                                                                                                          cgacgaCGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTc             >  2:4129363/1‑150 (MQ=255)

                                                                                                                                             cgacgCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAGc          <  1:5478592/150‑1 (MQ=255)

                                                                                                                                               tcgCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAGCtc        >  2:1837835/2‑150 (MQ=255)

                                                                                                                                               acgCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAGCtc        <  1:2726048/150‑1 (MQ=255)

                                                                                                                                                cgCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAGCtcg       >  2:4004059/1‑150 (MQ=255)

                                                                                                                                                 gCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAGCtcgt      >  2:4389624/1‑150 (MQ=255)

                                                                                                                                                  ccACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAGCtcgtc     <  1:3445113/150‑1 (MQ=255)

                                                                                                                                                   cACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAGCtcgtcg    >  2:3647905/1‑150 (MQ=255)

                                                                                                                                                    aCTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAGCTCGTCgg   <  2:3101331/150‑1 (MQ=255)

                                                                                                                                                    aCTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAGCTCGTCgg   <  2:3101128/150‑1 (MQ=255)

                                                                                                                                                     cTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAGCTCGTCggg  <  1:4777571/150‑1 (MQ=255)

TTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAGCTCGTCGGG  >  NC_002947/2144480‑2144778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`