Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,278,866 G→C 5.9% P165A (CCG→GCG) ‡ PP_2004 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,278,8660GC 5.9% 1079.1 / 42.6 320P165A (CCG→GCG) ‡PP_2004AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (137/164);  new base C (11/8);  total (148/172)
Fisher's exact test for biased strand distribution p-value = 3.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 333 total aligned reads displayed.

CCCGGGGTTGGCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGT  >  NC_002947/2278717‑2279015
                                                                                                                                                     |                                                                                                                                                     
cccGGGGTTGGCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCgg                                                                                                                                                       <  1:3588291/150‑1 (MQ=255)
cccGGGGTAGGCAAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCAGTCGGCGGCGAACTGCACAGGCGTGCTGAAAAGCGGTTGATAGCGCGCGGAATAGTAGGGCGCGTCAAACTCGATCTCAACGCGTTCGGACTGCACCgg                                                                                                                                                       >  2:4616723/1‑150 (MQ=255)
        tGGCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCgg                                                                                                                                               <  2:3088506/150‑1 (MQ=255)
        tGGCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCgg                                                                                                                                               <  1:4183357/150‑1 (MQ=255)
         ggCCAGTTGCACGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCGGCACCCGGGTGCAGGc                                                                                                                                              <  2:789576/150‑1 (MQ=255)
         ggCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGc                                                                                                                                              <  1:1504439/150‑1 (MQ=255)
         ggCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGc                                                                                                                                              <  1:4109593/150‑1 (MQ=255)
         ggCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCCCGGTGCAGGc                                                                                                                                              <  1:1660183/150‑1 (MQ=255)
           ccAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCa                                                                                                                                            <  1:1277975/150‑1 (MQ=255)
            cAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAg                                                                                                                                           >  1:3143748/1‑150 (MQ=255)
            cAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCGGCACCCCGGTGCAGGccga                                                                                                                                           <  2:932454/150‑3 (MQ=255)
              gTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCGGCACCCCGGTGCAGGccgaac                                                                                                                                         <  1:2451044/150‑5 (MQ=255)
                tGCAAGGTAGCGCGGCGCAGACGCAGCGGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCg                                                                                                                                       <  2:5518880/150‑1 (MQ=255)
                  cAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGc                                                                                                                                     >  1:4969528/1‑150 (MQ=255)
                     ggTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCaa                                                                                                                                  >  2:2984425/1‑150 (MQ=255)
                         gcgcGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTgg                                                                                                                              <  2:2349556/150‑1 (MQ=255)
                         gcgcGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTgg                                                                                                                              <  1:4474659/150‑1 (MQ=255)
                           gcgGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGgc                                                                                                                            >  2:859527/1‑150 (MQ=255)
                               cTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGgccagc                                                                                                                        >  1:551627/1‑150 (MQ=255)
                                tCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGgccagcc                                                                                                                       >  2:741122/1‑150 (MQ=255)
                                tCAGACGCAGCTGATTGCCGAAGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGACGGACAGCTCGGCCAACTGGgccagcc                                                                                                                       >  2:2594/1‑150 (MQ=255)
                                  aGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGccagccag                                                                                                                     >  2:2631083/1‑150 (MQ=255)
                                  aGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGccagccag                                                                                                                     >  1:4620972/1‑150 (MQ=255)
                                  aGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGccagccag                                                                                                                     <  1:3699334/150‑1 (MQ=255)
                                     cGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCTTAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCg                                                                                                                  <  2:671875/150‑1 (MQ=255)
                                     cGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCg                                                                                                                  <  2:1761363/150‑1 (MQ=255)
                                     cGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCg                                                                                                                  <  2:3657852/150‑1 (MQ=255)
                                        aGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGcc                                                                                                               >  2:1198178/1‑150 (MQ=255)
                                        aGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTGCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGcc                                                                                                               >  1:710629/1‑150 (MQ=255)
                                         gCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCca                                                                                                              <  2:905715/150‑1 (MQ=255)
                                          cTGATTGCCGTCGGCGGCGAACGGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCcag                                                                                                             <  2:1926207/150‑1 (MQ=255)
                                           tGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCTGGCTGGCCCAGTTGGCCGAGCTGGCCGGcaccc                                                                                                            <  2:2203944/150‑5 (MQ=37)
                                              ttGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGt                                                                                                         <  1:4758736/150‑1 (MQ=255)
                                                  cGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGt                                                                                                     >  2:4512381/1‑150 (MQ=255)
                                                   gTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTc                                                                                                    >  2:2444952/1‑150 (MQ=255)
                                                       gcggcgAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGAcc                                                                                                >  2:4100907/1‑150 (MQ=255)
                                                       gcggcgAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGAcc                                                                                                <  1:2631083/150‑1 (MQ=255)
                                                        cggcgAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACca                                                                                               <  2:4584845/150‑1 (MQ=255)
                                                         ggcgAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACcac                                                                                              <  2:5165344/150‑1 (MQ=255)
                                                                tGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCgg                                                                                       >  1:4415047/1‑150 (MQ=255)
                                                                 gCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGt                                                                                      >  1:4847375/1‑150 (MQ=255)
                                                                 gCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGt                                                                                      >  1:2015411/1‑150 (MQ=255)
                                                                  cacaGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGtt                                                                                     <  2:4272247/150‑1 (MQ=255)
                                                                  cacaGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGtt                                                                                     <  1:521557/150‑1 (MQ=255)
                                                                  cacaGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGtt                                                                                     >  1:3751252/1‑150 (MQ=255)
                                                                  cacaGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGtt                                                                                     <  1:795008/150‑1 (MQ=255)
                                                                  cacaGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGtt                                                                                     >  2:5050344/1‑150 (MQ=255)
                                                                      ggCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAg                                                                                 <  2:3482705/150‑1 (MQ=255)
                                                                           gctgcAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGtt                                                                            <  1:2777747/150‑1 (MQ=255)
                                                                               cAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCa                                                                                                                                            >  1:5296247/1‑82 (MQ=255)
                                                                               cAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAg                                                                        <  2:3696887/150‑1 (MQ=255)
                                                                                aaaGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAgg                                                                       >  2:1507347/1‑150 (MQ=255)
                                                                                aaaGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAgg                                                                       >  2:718559/1‑150 (MQ=255)
                                                                                       ttGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGAt                                                                >  1:5356603/1‑150 (MQ=255)
                                                                                           tAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGCTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGaa                                                            <  2:1480270/150‑1 (MQ=255)
                                                                                            aGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCCGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAAt                                                           >  1:5080062/1‑150 (MQ=255)
                                                                                             gcgcgcGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTGGTAGTCGTTGTAGGGGCTGATGGAATa                                                          >  1:5153106/1‑150 (MQ=255)
                                                                                               gcgcgGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCTGCCAGCTCGGCCAACTGGGCCAGCCAGCCGACCAGCAGTGAGTCGACCACAAAGCGGATGTAGTCGTTGTAGGGGCTGATGGAATAGa                                                        >  2:5419865/1‑150 (MQ=255)
                                                                                               gcgcgGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGa                                                        <  2:4822119/150‑1 (MQ=255)
                                                                                                cgcgGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGaa                                                       <  1:1218340/150‑1 (MQ=255)
                                                                                                cgcgGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGaa                                                       <  1:3509412/150‑1 (MQ=255)
                                                                                                   ggCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAgcg                                                    >  1:225399/1‑150 (MQ=255)
                                                                                                     cATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCa                                                  >  2:2019293/1‑150 (MQ=255)
                                                                                                      aTAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAg                                                 <  2:1066124/150‑1 (MQ=255)
                                                                                                          gaggGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCt                                                                   <  1:859954/128‑1 (MQ=255)
                                                                                                          gaggGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAg                                             <  2:5241301/150‑1 (MQ=255)
                                                                                                               cgcgGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAgg                                                                       <  2:3891466/119‑1 (MQ=255)
                                                                                                               cgcgGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAgg                                                                       >  1:3891466/1‑119 (MQ=255)
                                                                                                                  ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCCGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTg                                     >  1:3229269/1‑150 (MQ=255)
                                                                                                                      aaCTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCa                                 >  2:3225301/1‑150 (MQ=255)
                                                                                                                       aCTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCAt                                <  1:1409159/150‑1 (MQ=255)
                                                                                                                        cTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGAt                                                                >  1:284517/1‑117 (MQ=255)
                                                                                                                        cTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGAt                                                                <  2:284517/117‑1 (MQ=255)
                                                                                                                         tCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTg                                     <  2:3641149/143‑1 (MQ=255)
                                                                                                                         tCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTg                                     >  1:3641149/1‑143 (MQ=255)
                                                                                                                         tCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCt                              >  2:2334881/1‑150 (MQ=255)
                                                                                                                           gATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTc                            >  2:5268904/1‑150 (MQ=255)
                                                                                                                               tcCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCa                        >  2:1260215/1‑150 (MQ=255)
                                                                                                                                ccAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCaa                       >  2:3179179/1‑150 (MQ=255)
                                                                                                                                ccAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCACGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCaa                       <  2:5598933/150‑1 (MQ=255)
                                                                                                                                 cAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATATAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCaaa                      >  1:318703/1‑150 (MQ=255)
                                                                                                                                 cAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCaaa                      <  2:1088197/150‑1 (MQ=255)
                                                                                                                                 cAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCaaa                      >  1:3350149/1‑150 (MQ=255)
                                                                                                                                     gagctggCCGGCACCCCGGTGCAGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCgg                  >  2:163645/6‑150 (MQ=255)
                                                                                                                                     gagctggCCGGCACCCCGGTGCAGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCgg                  >  1:1745157/6‑150 (MQ=255)
                                                                                                                                     gagctggCCGGCACCCCGGTGCAGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCgg                  >  1:4674567/6‑150 (MQ=255)
                                                                                                                                        ctggCCGGCACCCCGGTGCAGGCCGGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGaa               >  1:2535308/3‑150 (MQ=255)
                                                                                                                                         cGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAAt              <  2:61342/150‑1 (MQ=255)
                                                                                                                                         cGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAAt              <  1:2318931/150‑1 (MQ=255)
                                                                                                                                         cGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAAt              <  1:2041473/150‑1 (MQ=255)
                                                                                                                                          ggCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCCGCCGGCCAGCAGGGAGTCGACCACAAAGCGGTTGTAGTCGTGGTAGGGGCTGATGGACTAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCACAGCGGGAATg             >  1:193580/1‑150 (MQ=255)
                                                                                                                                          ggCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATg             >  1:3008973/1‑150 (MQ=255)
                                                                                                                                           gCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCCGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATgg            >  1:1326872/1‑150 (MQ=255)
                                                                                                                                                 aCCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCgg      >  1:2164747/1‑150 (MQ=255)
                                                                                                                                                  ccGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGt     >  2:1591050/1‑150 (MQ=255)
                                                                                                                                                   cGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTa    <  2:5475829/150‑1 (MQ=255)
                                                                                                                                                   cGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTa    >  1:4526154/1‑150 (MQ=255)
                                                                                                                                                    ggggTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCACAGCGGGAATGGCCGCGGTAg   <  2:5128166/150‑1 (MQ=255)
                                                                                                                                                    ggggTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAg   <  2:1868280/150‑1 (MQ=255)
                                                                                                                                                     gggTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGt  >  2:1388508/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCCGGGGTTGGCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGT  >  NC_002947/2278717‑2279015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑