BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,737,574	T→G	6.2%	V640G (GTG→GGG)	polB →	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,737,574	0	T	G	6.2%	596.7 / 2.6	219	V640G (GTG→GGG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand): ref base T (77/122); new base G (16/4); total (93/126)
Fisher's exact test for biased strand distribution p-value = 5.54e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

CGCCCTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGC  >  NC_002947/2737425‑2737723

 gcCCTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTg                                                                                                                                                      <  1:450531/150‑1 (MQ=255)

  cccTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTgc                                                                                                                                                     <  2:2695575/150‑1 (MQ=255)

  accTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGGgc                                                                                                                                                     >  2:1553121/2‑150 (MQ=255)

   ccTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTgcg                                                                                                                                                    >  1:4441786/1‑150 (MQ=255)

   ccTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTgcg                                                                                                                                                    >  2:4004050/1‑150 (MQ=255)

    cTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTgcgc                                                                                                                                                   >  2:2278108/1‑150 (MQ=255)

    cTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTgcgc                                                                                                                                                   >  2:4193663/1‑150 (MQ=255)

    cTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTgcgc                                                                                                                                                   >  2:4193709/1‑150 (MQ=255)

     tCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTACTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTgcgcc                                                                                                                                                  >  1:4897290/1‑149 (MQ=255)

       gaccTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCAcc                                                                                                                                                <  1:497768/147‑1 (MQ=255)

        aaCTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCg                                                                                                                                               <  1:3972023/150‑1 (MQ=255)

           tGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACt                                                                                                                                            <  1:871997/150‑1 (MQ=255)

            gctgTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACtc                                                                                                                                           <  2:1847851/147‑2 (MQ=255)

            gCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTg                                                                                                                                           >  2:3468450/1‑150 (MQ=255)

            gCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCAGCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCGGGTGCAGCGGGCCGACGGTGCTGAAGAAAGGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTg                                                                                                                                           <  1:4521345/150‑1 (MQ=255)

             cAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTgg                                                                                                                                          >  2:5455546/1‑150 (MQ=255)

               tttCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTc                                                                                                                                        <  2:559440/149‑1 (MQ=255)

                ttCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTTTGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGGGCGCCCCGACAggggc                                                                                                                                       >  1:533476/1‑147 (MQ=255)

                   gAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACAGGTCACCg                                                                                                                                    >  2:4466141/1‑150 (MQ=255)

                    aGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGGGCGCCCCGCCAGGTCACCGc                                                                                                                                   >  1:3609026/1‑150 (MQ=255)

                      gTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTg                                                                                                                                 <  1:894397/150‑1 (MQ=255)

                       tGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTgg                                                                                                                                <  2:5406321/150‑1 (MQ=255)

                          aCTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGccc                                                                                                                             <  1:2905309/150‑1 (MQ=255)

                            tACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCgg                                                                                                                           <  2:1315203/150‑1 (MQ=255)

                            gaCCGGCGCTGCCTGATGCCCACCATCCGCGGCACCGCCGAGGGCAGCACGAAGCGCTAGGCCGGCCGGGGGCAGCGGGCCGCCGGTGCTGAAGAAATGGTTTACACGGGCCTGGCGTCGGTGCGCACCGACTGGTCACCGCTGGCCCgg                                                                                                                           <  1:1170988/149‑1 (MQ=255)

                              ccGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTTCGCACCGACTGGTCACCGCTGGCCCGGcc                                                                                                                         >  2:1937262/1‑149 (MQ=255)

                              ccGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCa                                                                                                                         <  2:1357229/150‑1 (MQ=255)

                                ggCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTGACCGCTGGCCCGGCAGt                                                                                                                       >  2:3616206/1‑150 (MQ=255)

                                     ttCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCcag                                                                                                                  >  2:4621583/1‑150 (MQ=255)

                                        cTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCcagcag                                                                                                               <  2:3170604/150‑1 (MQ=255)

                                         tGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGGGGGCCCCCACAGGTCACCCCGGGCCCGGCCGGTCCAGCAgg                                                                                                              >  1:1555487/1‑150 (MQ=255)

                                         tGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGGGCGCCCCGCCTGGTCACCGCTGGCCCGGCCGGTCCAGCAgg                                                                                                              >  1:1067774/1‑150 (MQ=255)

                                         tGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGGGCGCCCCGCCTGGTCACCCCTGGCCCGGCCGTTCCAGCAgg                                                                                                              >  1:1067616/1‑150 (MQ=255)

                                           aTGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGaa                                                                                                            <  1:2747103/150‑1 (MQ=255)

                                             gCCCACCATCCGCGGCACCGACGCGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACACGGGCCTGGCGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAAct                                                                                                          <  1:265156/150‑1 (MQ=255)

                                              cccACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGGGCGCCCCCACGCGTCACCCCGGGCCCCGCCGGTCCAGCAGGAActc                                                                                                         >  1:1979328/1‑150 (MQ=255)

                                               ccaccaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCCACAGGTCACCGCAGGCCCGGCCCGTCCAGCAGGAAcgcg                                                                                                        >  1:516979/1‑147 (MQ=255)

                                                 cccaTCCGCGGCACCGACGAGGGCAGAAAGATGCGCTAGGCCGGCCTGGTGCAGCGGGCCGAGGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGGCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCTGCTGGAActgccg                                                                                                      <  1:1389463/149‑5 (MQ=255)

                                                 accaTCCGCGGCACCGACGAGGGCATCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTAc                                                                                                      <  1:486694/150‑1 (MQ=255)

                                                  ccaTCCGCGGCACCGCCGAGGGCAGCAAGACGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCTGCTGGAActgccgg                                                                                                     <  2:1503993/150‑6 (MQ=255)

                                                  ccaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACACGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCTGCTGGAActgccgg                                                                                                     <  1:1227322/150‑6 (MQ=255)

                                                  ccaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCCCCGACTGGTCACCGCAGGCCCGGCAGTTCCAGCAGGAACTCTACg                                                                                                     >  1:4126159/1‑150 (MQ=255)

                                                    aTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGc                                                                                                   <  2:260863/150‑1 (MQ=255)

                                                     tCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACACGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGcc                                                                                                  <  1:2331565/150‑1 (MQ=255)

                                                       cgcgGCACCGACGAGGGCAGCAAGAAGCGCTTTGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCgt                                                                                                <  1:2142322/150‑1 (MQ=255)

                                                       cgcgGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAATTGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCgt                                                                                                <  1:45950/150‑1 (MQ=255)

                                                         cgGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCgtgt                                                                                              <  2:4814039/150‑1 (MQ=255)

                                                         cgGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCAGGCCCCGCAGTTCCCGCAGGCACACAACGGCCgggg                                                                                              >  1:4031905/1‑147 (MQ=255)

                                                            cACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGAGGGTGCTGAAGAAATGTTTTACACGGGCCTGGAGTCGGTGCGCCCCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGtt                                                                                           <  1:948234/150‑1 (MQ=255)

                                                            cACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGtt                                                                                           <  1:2703446/150‑1 (MQ=255)

                                                                 ccgaGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTCAAGAATTGGTTTACACGGGCCGGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCa                                                                                      <  1:375660/149‑1 (MQ=255)

                                                                 acgaGGGCCGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTCCCAGGGCCTGGAGTCGGTGCGCACCGCCTGGTCACCCCAGGCCCGGCAGTTCCAGCAGGAACTCTCCGGCCGGGGGTTCCGCa                                                                                      >  2:519913/1‑150 (MQ=255)

                                                                 acgaGGCCACCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAACAAATGTTTTACAAGGGCCTGGAGTCGGTGCGCACCCACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCa                                                                                      <  1:3453661/150‑1 (MQ=255)

                                                                   gaGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCAACGACTGGTAACCGCTGGCACGGCAGTTACATCATGAACTCTACGGCCGTGTGTTACGCAGc                                                                                    >  2:908140/1‑150 (MQ=255)

                                                                    aGGGCAGCAAGAAGCCCTCTGCCGGCCTGGTGCAGCGGCCCGACGGCGCTGAAGAAATGTTTTCCAAGGGCCTGGAGTCGGTCCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCg                                                                                   <  1:2020886/150‑1 (MQ=255)

                                                                      ggCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTAACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAg                                                                                 >  2:2715124/1‑150 (MQ=255)

                                                                               aagCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAACGAGCCCTACCGt                                                                        >  2:199379/1‑150 (MQ=255)

                                                                                agCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGGGCGCCCCGCCGGGGCACCCCGGGCCCGGCCCGTCCAGCAGGACCACTACGGCCGGGTGTTACGCCGCCAGCCCGACCggg                                                                       >  1:996328/1‑148 (MQ=255)

                                                                                   gcTATGCCGGCCTGGTGCAGCGGCCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGAtt                                                                    <  2:247730/150‑1 (MQ=255)

                                                                                     tATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTTAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTAt                                                                  <  1:440765/150‑1 (MQ=255)

                                                                                          cGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGGGCACCCACGGGTCACCGCGGGCCCCGCAGGTACACCAGGGACACGACGGCCGGGTGTTACGCAGCGAGCCCGACCCGGATTATGTAcg                                                             >  1:3145576/1‑150 (MQ=255)

                                                                                          cGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCCCCGACGGGTCACCCCAGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTGCGCAGCCAGCCCTACCGTGATTATGTAcg                                                             >  1:1825984/1‑150 (MQ=255)

                                                                                          cGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGGGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCcg                                                             >  2:1794020/1‑150 (MQ=255)

                                                                                             ccTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGa                                                          <  2:4321060/150‑1 (MQ=255)

                                                                                             ccTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGa                                                          <  2:2661032/150‑1 (MQ=255)

                                                                                                      gCGGGCCGCCGGTGCGGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTGCGCGAGTAGGTACg                                                 >  2:207776/1‑150 (MQ=255)

                                                                                                           ccGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCa                                            <  2:5565929/150‑1 (MQ=255)

                                                                                                           ccGACGGTGCGGAAGACAGGGTTAACACGGGCCTGGAGGCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCa                                            <  1:3342021/150‑1 (MQ=255)

                                                                                                             gACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACg                                          >  2:2835962/1‑150 (MQ=255)

                                                                                                                ggTGCTGAAGACATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTg                                       <  2:5095923/150‑1 (MQ=255)

                                                                                                                  tGCTGATGAAATGTTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggc                                     <  1:5601114/150‑1 (MQ=255)

                                                                                                                  tGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggc                                     >  2:3720058/1‑150 (MQ=255)

                                                                                                                  tGCTGAAGAAATGGTTTACAAGGGCCTCGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggc                                     <  2:3662568/150‑1 (MQ=255)

                                                                                                                   gCTGACGAACTGGTTTACAAGGGCCTGGAGCCGGGGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggcg                                    <  1:2312985/150‑1 (MQ=255)

                                                                                                                   gCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggcg                                    <  2:4510551/150‑1 (MQ=255)

                                                                                                                     tGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggcggg                                  >  2:5294886/1‑150 (MQ=255)

                                                                                                                     tGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggcggg                                  >  2:97162/1‑150 (MQ=255)

                                                                                                                     tGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCCTGATTATGTCCGCGAGTATGTACGGCGCACGTTggcggg                                  >  2:270013/1‑150 (MQ=255)

                                                                                                                       aagaaATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggcgggcg                                >  2:141937/1‑150 (MQ=255)

                                                                                                                          acaTGGTTGACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGc                             <  2:4400276/148‑1 (MQ=255)

                                                                                                                           actgttttACATGGGCCGGGCGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCa                            <  1:4789591/145‑1 (MQ=255)

                                                                                                                           aaTGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCa                            <  1:1707570/150‑1 (MQ=255)

                                                                                                                           aaTGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACACAACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCa                            >  2:5145438/1‑150 (MQ=255)

                                                                                                                                   ccacgggCGTGGAGTCGGTGCGCCCCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAAc                    <  1:1029682/146‑1 (MQ=255)

                                                                                                                                     aaGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAActg                  >  2:3259878/1‑150 (MQ=255)

                                                                                                                                       gggCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAActgct                <  2:533476/150‑1 (MQ=255)

                                                                                                                                       gggCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAActgct                <  2:2329197/150‑1 (MQ=255)

                                                                                                                                       gggCCTGGAGTCGGTGCGCACCGACTGGTCACCCCTGGCCCGGCAGTTCCAGCAGGAACACAACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAActgcg                >  2:663018/1‑149 (MQ=255)

                                                                                                                                         gCCTGGCGGCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTgg              <  1:4294862/150‑1 (MQ=255)

                                                                                                                                         gCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTgg              <  1:5455546/150‑1 (MQ=255)

                                                                                                                                         gCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTgg              >  2:765857/1‑150 (MQ=255)

                                                                                                                                          ccTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTCCGGCCGTGTGTGCCGCAGCGAGCCCGACCGTGATTATGTCCGCGAGTCTGGACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGt             <  2:4949053/150‑1 (MQ=255)

                                                                                                                                              caGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTAt         >  2:2984774/2‑150 (MQ=255)

                                                                                                                                              caGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTAt         >  2:1120133/2‑150 (MQ=255)

                                                                                                                                               tgTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATc        <  1:1620200/149‑1 (MQ=255)

                                                                                                                                               aGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATc        <  2:3804781/150‑1 (MQ=255)

                                                                                                                                               aGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATc        <  2:1251198/150‑1 (MQ=255)

                                                                                                                                               aGTCGGTGCCCACCCACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATc        <  1:3573944/150‑1 (MQ=255)

                                                                                                                                                   ggTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCaa    <  2:1467134/150‑1 (MQ=255)

                                                                                                                                                   ggTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCACACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCaa    <  1:380356/150‑1 (MQ=255)

CGCCCTCGAACTGCAGTTCGAGGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGC  >  NC_002947/2737425‑2737723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`