BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,789,136	G→C	5.2%	Q199H (CAG→CAC)	PP_2437 →	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,789,136	0	G	C	5.2%	786.7 / 24.0	231	Q199H (CAG→CAC)	PP_2437	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (127/92); new base C (7/5); total (134/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 252 total aligned reads displayed.

GCCACCAACATGGCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGATCCTTGGTG  >  NC_002947/2788987‑2789284

  caccaACATGGCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAgcagca                                                                                                                                                    >  2:3923922/1‑150 (MQ=255)

  caccaACATGGCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAgcagca                                                                                                                                                    >  2:1807899/1‑150 (MQ=255)

  caccaACATGGCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAgcagca                                                                                                                                                    >  2:5532758/1‑150 (MQ=255)

  caccaACATGGCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAgcagca                                                                                                                                                    >  2:1424657/1‑150 (MQ=255)

  caccaACATGGCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAgcagca                                                                                                                                                    >  1:2153764/1‑150 (MQ=255)

       aCATGGCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCa                                                                                                                                               >  2:3332339/1‑150 (MQ=255)

       aCATGGCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCa                                                                                                                                               <  1:5475589/150‑1 (MQ=255)

         aTGGCTGCTACCGCCATACGGGATGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCAtg                                                                                                                                             <  1:2206112/150‑1 (MQ=255)

          tGGCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCAtgg                                                                                                                                            >  2:2416546/1‑150 (MQ=255)

          tGGCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCAtgg                                                                                                                                            <  2:1148249/150‑1 (MQ=255)

          tGGCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCAtgg                                                                                                                                            <  1:4601868/150‑1 (MQ=255)

           ggCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCAgggt                                                                                                                                           >  2:4317288/1‑150 (MQ=255)

            gctgctACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCAtg                                                                                                                                             <  2:5225367/147‑1 (MQ=255)

              tgctACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCAtggtgct                                                                                                                                        >  2:3001661/1‑150 (MQ=255)

               gctACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCAtggtgctg                                                                                                                                       >  1:4025805/1‑150 (MQ=255)

               gctACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCACCATGGAAtgctgctg                                                                                                                                       <  1:1922701/150‑1 (MQ=255)

               gctACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCACCATGGAAtgctgctg                                                                                                                                       <  1:1822978/150‑1 (MQ=255)

                 tACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCAtggtgctggt                                                                                                                                     >  2:270960/1‑150 (MQ=255)

                  aCCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCAtggtgctggtg                                                                                                                                    <  1:1785657/150‑1 (MQ=255)

                   ccGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCAtggtgctggtgc                                                                                                                                   >  1:1318014/1‑150 (MQ=255)

                    cGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGcc                                                                                                                                  >  2:5397575/1‑150 (MQ=255)

                          aCGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGccgaccga                                                                                                                            >  1:897008/1‑150 (MQ=255)

                           cGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCcgaccgac                                                                                                                           <  1:5224892/150‑1 (MQ=255)

                            gggACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGaca                                                                                                                          >  2:1679844/1‑150 (MQ=255)

                              gACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACg                                                                                                                        >  2:2825386/1‑150 (MQ=255)

                                    gACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGc                                                                                                                  <  1:662151/150‑1 (MQ=255)

                                    gACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGc                                                                                                                  >  1:5491330/1‑150 (MQ=255)

                                     aCCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCg                                                                                                                 <  2:3932971/150‑1 (MQ=255)

                                      ccAGTGGGGGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCCGCGACCCGCGCTGCAAGGTCATGATCTTCATCGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCAGTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGt                                                                                                                <  2:3857589/150‑1 (MQ=255)

                                       cAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTg                                                                                                               >  1:5239933/1‑150 (MQ=255)

                                        aGTGGGTGATCAACGGCCGCTAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCAGACCGACACGCCGGGCGTGa                                                                                                              <  2:4114263/150‑1 (MQ=255)

                                        aGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGa                                                                                                              <  1:3631737/150‑1 (MQ=255)

                                            ggTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGAt                                                                                                          >  2:2760496/1‑150 (MQ=255)

                                             gTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATc                                                                                                         >  1:2714262/1‑150 (MQ=255)

                                               gATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGt                                                                                                       >  2:3030654/1‑150 (MQ=255)

                                                 tCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTcc                                                                                                     >  2:5244302/1‑150 (MQ=255)

                                                   aaCGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTccgc                                                                                                   >  2:4934513/1‑150 (MQ=255)

                                                   aaCGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTccgc                                                                                                   >  2:3401130/1‑150 (MQ=255)

                                                          gCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGc                                                                                            <  1:3556511/150‑1 (MQ=255)

                                                                 gtggACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGtt                                                                                     >  2:4410784/1‑150 (MQ=255)

                                                                  tggACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTc                                                                                    >  1:1034515/1‑150 (MQ=255)

                                                                   ggACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCg                                                                                   >  2:4008537/1‑150 (MQ=255)

                                                                   ggACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCg                                                                                   >  1:775501/1‑150 (MQ=255)

                                                                    gACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCgg                                                                                  <  1:4773668/150‑1 (MQ=255)

                                                                    gACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCgg                                                                                  <  1:4266704/150‑1 (MQ=255)

                                                                     aCGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGt                                                                                 <  2:709605/150‑1 (MQ=255)

                                                                      cGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGtt                                                                                <  2:5023961/150‑1 (MQ=255)

                                                                      cGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGtt                                                                                <  2:2587732/150‑1 (MQ=255)

                                                                      cGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGtt                                                                                >  1:2828176/1‑150 (MQ=255)

                                                                        tCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTAt                                                                              <  1:2758033/150‑1 (MQ=255)

                                                                            gcgcCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGGTCGGTTATGACg                                                                          >  2:2092093/1‑150 (MQ=255)

                                                                              gcCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGAt                                                                        >  1:5019132/1‑150 (MQ=255)

                                                                                cTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATgc                                                                      >  1:4835110/1‑150 (MQ=255)

                                                                                 tGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTCTGACGATgcg                                                                     >  1:4234905/1‑150 (MQ=255)

                                                                                 tGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCGGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCGGCCGGTGTTCGGTTATGACGGTgcg                                                                     >  1:2527519/1‑150 (MQ=255)

                                                                                     aCCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCCGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGcccc                                                                 >  1:1349324/1‑150 (MQ=255)

                                                                                       ccGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCAt                                                               >  2:46307/1‑150 (MQ=255)

                                                                                        cgcgCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATg                                                              <  1:3346522/150‑1 (MQ=255)

                                                                                        cgcgCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATg                                                              <  1:4439741/150‑1 (MQ=255)

                                                                                         gcgcTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATgg                                                             >  2:955994/1‑150 (MQ=255)

                                                                                         gcgcTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATgg                                                             <  2:1382432/150‑1 (MQ=255)

                                                                                           gcTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTc                                                           <  2:2673976/150‑1 (MQ=255)

                                                                                               cAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGc                                                       >  2:2162269/1‑150 (MQ=255)

                                                                                               cAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGc                                                       >  2:2311742/1‑150 (MQ=255)

                                                                                               cAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGc                                                       <  2:1228323/150‑1 (MQ=255)

                                                                                                aaGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGcc                                                      >  2:1995393/1‑150 (MQ=255)

                                                                                                   gTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGaa                                                   <  2:1012893/150‑1 (MQ=255)

                                                                                                    tCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAg                                                  >  1:3302044/1‑150 (MQ=255)

                                                                                                    tCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGGTATGACGATGCGCCCCATGGTCATGCCGAAg                                                  >  2:311930/1‑150 (MQ=255)

                                                                                                      aTGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCAtggtgctggt                                                                                                                                     >  1:2804593/1‑65 (MQ=255)

                                                                                                         aTCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCtt                                             <  1:1995393/150‑1 (MQ=255)

                                                                                                         aTCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCtt                                             <  2:2453216/150‑1 (MQ=255)

                                                                                                          tCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCttt                                            >  1:2969406/1‑150 (MQ=255)

                                                                                                            ttCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTc                                          <  1:3190062/150‑1 (MQ=255)

                                                                                                                    ccTGTCTGACCCTGAAGGGCCACGCAACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAAtgt                                  >  1:184370/1‑150 (MQ=255)

                                                                                                                      tGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGACTGTGc                                >  2:3776448/1‑150 (MQ=255)

                                                                                                                      tGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGc                                <  1:3986197/150‑1 (MQ=255)

                                                                                                                        tCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCGTGCCGAAGTCCTTTTCGAGAATGTGCgg                              >  1:374091/1‑150 (MQ=255)

                                                                                                                        tCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGACTGTGCgg                              >  2:3493811/1‑150 (MQ=255)

                                                                                                                         cTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCggg                             <  1:704991/150‑1 (MQ=255)

                                                                                                                            aCCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGc                          >  1:1700615/1‑150 (MQ=255)

                                                                                                                              ccTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGGTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCa                        >  2:866528/1‑150 (MQ=255)

                                                                                                                                tGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATa                      >  1:4429592/1‑150 (MQ=255)

                                                                                                                                tGAAGGGCCACACCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATa                      >  1:2904485/1‑150 (MQ=255)

                                                                                                                                      gccacgccacCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGaaaa                <  1:60139/150‑1 (MQ=255)

                                                                                                                                      gccacgccacCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATAGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGACTAAGTCCTTATCGAGAATGTGCGGGAGCCATAAGaaaa                >  1:115363/1‑150 (MQ=255)

                                                                                                                                       ccacgccacCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAAt               <  2:387468/150‑1 (MQ=255)

                                                                                                                                       ccacgccacCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAAt               >  2:3654126/1‑150 (MQ=255)

                                                                                                                                           ggcaccaGCACCATGGAATGCTGCTGGTGGCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGa           >  2:4577933/3‑150 (MQ=255)

                                                                                                                                           gccacCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGa           <  1:2416546/150‑1 (MQ=255)

                                                                                                                                           gccacCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGa           <  2:1965827/150‑1 (MQ=255)

                                                                                                                                           gccacCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGa           <  2:360685/150‑1 (MQ=255)

                                                                                                                                             caccaGCACCATGGAATGCTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGATc         >  1:2022370/1‑150 (MQ=255)

                                                                                                                                              accaGCACCATGGAATGCTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGATcc        >  2:3393189/1‑150 (MQ=255)

                                                                                                                                               ccaGCACCATGGAATGCTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGATCCt       >  1:1460210/1‑150 (MQ=255)

                                                                                                                                                 agcagcATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGATCCTTg     <  1:3829807/150‑1 (MQ=255)

                                                                                                                                                 agcagcATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGATCCTTg     <  2:2219930/150‑1 (MQ=255)

                                                                                                                                                  gcagcaTTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGATCCTTgg    <  1:5627040/150‑1 (MQ=255)

                                                                                                                                                    agcaTTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGATCCTTGGTg  >  1:5346921/1‑150 (MQ=255)

                                                                                                                                                    agcaTTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGATCCTTGGTg  >  1:449913/1‑150 (MQ=255)

GCCACCAACATGGCTGCTACCGCCATACGGGACGGTGACCAGTGGGTGATCAACGGCCGCAAGTGGTGGACGTCCGGCGCCTGCGACCCGCGCTGCAAGGTCATGATCTTCATGGGCCTGTCTGACCCTGAAGGGCCACGCCACCAGCAGCATTCCATGGTGCTGGTGCCGACCGACACGCCGGGCGTGAAGATCGTCCGCCCGCTGCCGGTGTTCGGTTATGACGATGCGCCCCATGGTCATGCCGAAGTCCTTTTCGAGAATGTGCGGGTGCCATACGAAAATGTGATCCTTGGTG  >  NC_002947/2788987‑2789284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`