Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,576,669 C→G 5.1% R236G (CGC→GGC)  yneJ → LysR family DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,576,6690CG 5.1% 745.1 / 19.4 219R236G (CGC→GGC) yneJLysR family DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (111/96);  new base G (3/9);  total (114/105)
Fisher's exact test for biased strand distribution p-value = 7.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

CGCCTTCCGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCTTGAAGAAGG  >  NC_002947/3576521‑3576817
                                                                                                                                                    |                                                                                                                                                    
cGCCTTCCGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCcgcg                                                                                                                                                     >  2:2190292/1‑150 (MQ=255)
cGCCTTCCGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCcgcg                                                                                                                                                     >  1:231350/1‑150 (MQ=255)
   cTTCCGCGCCAACTGTTCATACCGCCATCACGTCGAACACTGGGTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCgcag                                                                                                                                                  <  2:64099/150‑1 (MQ=255)
    ttCCGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCgcagc                                                                                                                                                 >  1:585709/1‑150 (MQ=255)
    ttCCGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCgcagc                                                                                                                                                 <  2:4700653/150‑1 (MQ=255)
    ttCCGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCgcagc                                                                                                                                                 <  2:1867208/150‑1 (MQ=255)
      ccGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCGCGGCAGCAt                                                                                                                                               <  2:3992804/150‑1 (MQ=255)
       cgcgCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCGCGGCAGCATg                                                                                                                                              <  2:3743354/150‑1 (MQ=255)
         cgcCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGGGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCGCGGCAGCATggc                                                                                                                                            <  1:5003578/150‑3 (MQ=255)
          gcCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCGCGGCAGCATggcc                                                                                                                                           <  2:691122/150‑4 (MQ=255)
          gcCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTc                                                                                                                                           <  1:3302118/150‑1 (MQ=255)
          gcCAACTGTTCATACCGCCATCACTTCGAAAACTGGGTCGTGCAGGACCAGGCGGGACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTc                                                                                                                                           <  1:3644862/150‑1 (MQ=255)
           ccAACTCATCATACCGCCATCACGTCCAAAACAGGTTCCTCCAGGACCAGGCGGTACCGGGCAAGATTCACCAAATGGAGTCGTACCACGGCATGATGGCCTGCGACAGTGCGGGTGCCGGCCTGCCCATGCTGCGCGGCAGCATggcca                                                                                                                                          <  2:3211569/150‑5 (MQ=255)
             aaCTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGAc                                                                                                                                        >  1:2969259/1‑150 (MQ=255)
             aaCTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGAc                                                                                                                                        >  1:2969132/1‑150 (MQ=255)
                tGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAat                                                                                                                                     >  1:927483/1‑150 (MQ=255)
                tGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAat                                                                                                                                     >  1:2402974/1‑150 (MQ=255)
                  ttCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAatat                                                                                                                                   >  1:1471785/1‑150 (MQ=255)
                     aTACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTc                                                                                                                                           <  1:3241452/139‑1 (MQ=255)
                      tACCGCCATCACTTCGAAAACTGGGTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGccc                                                                                                                               <  1:3977736/150‑1 (MQ=255)
                       aCCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCg                                                                                                                              >  1:5495027/1‑150 (MQ=255)
                          gCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGt                                                                                                                           >  2:2775410/1‑150 (MQ=255)
                          gCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGt                                                                                                                           <  1:350510/150‑1 (MQ=255)
                                aCTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTgcagca                                                                                                                     >  2:4577005/1‑150 (MQ=255)
                                  ttCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCAcc                                                                                                                   >  1:5064467/1‑150 (MQ=255)
                                  ttCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCAcc                                                                                                                   >  2:1136964/1‑150 (MQ=255)
                                    cGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGTTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGt                                                                                                                 >  2:4934419/1‑150 (MQ=255)
                                     gAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTc                                                                                                                >  2:2709721/1‑150 (MQ=255)
                                           gtgTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGcc                                                                                                          >  2:1818086/3‑150 (MQ=255)
                                            ggTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCt                                                                                                         <  2:4649728/150‑1 (MQ=255)
                                             gTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTg                                                                                                        >  1:1036256/1‑150 (MQ=255)
                                             gTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTg                                                                                                        <  2:154878/150‑1 (MQ=255)
                                             gTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTg                                                                                                        >  2:5102145/1‑150 (MQ=255)
                                                 gTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCa                                                                                                    >  2:2623759/1‑150 (MQ=255)
                                                   gCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAAt                                                                                                  <  1:2480224/150‑1 (MQ=255)
                                                   gCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAAt                                                                                                  >  1:5544135/1‑150 (MQ=255)
                                                     aGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCAGGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGt                                                                                                <  2:4256895/150‑1 (MQ=255)
                                                      ggACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTc                                                                                               >  2:276174/1‑150 (MQ=255)
                                                      ggACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTc                                                                                               >  2:5184203/1‑150 (MQ=255)
                                                         ccAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCgga                                                                                            <  2:4019704/150‑1 (MQ=255)
                                                         ccAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCgga                                                                                            <  1:4286755/150‑1 (MQ=255)
                                                         ccAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCgga                                                                                            >  1:579766/1‑150 (MQ=255)
                                                          cAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCggag                                                                                           >  2:2040826/1‑150 (MQ=255)
                                                          cAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCggag                                                                                           >  2:1966011/1‑150 (MQ=255)
                                                                      ggCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTAt                                                                               >  2:2279627/1‑150 (MQ=255)
                                                                      ggCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTAt                                                                               <  1:4365309/150‑1 (MQ=255)
                                                                      ggCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTAt                                                                               <  1:3866780/150‑1 (MQ=255)
                                                                           caTTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATCCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGaa                                                                          <  1:3738472/149‑1 (MQ=255)
                                                                             ttCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGaaaa                                                                        <  1:4948974/150‑1 (MQ=255)
                                                                              tCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAAc                                                                       >  2:911227/1‑150 (MQ=255)
                                                                                aCGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACct                                                                     >  1:805381/1‑150 (MQ=255)
                                                                                aCGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACct                                                                     >  1:3441224/1‑150 (MQ=255)
                                                                                  gAAATGGAGTCGGACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACctgg                                                                   <  2:4246851/150‑1 (MQ=255)
                                                                                   aaaTGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACctggc                                                                  <  1:1792710/150‑1 (MQ=255)
                                                                                   aaaTGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACctggc                                                                  >  1:1510087/1‑150 (MQ=255)
                                                                                    aaTGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACctggct                                                                 <  1:1139553/150‑1 (MQ=255)
                                                                                      tGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACctggctgg                                                               <  1:1105782/150‑1 (MQ=255)
                                                                                       ggAGTCGTACAACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGt                                                              <  2:4188167/150‑1 (MQ=255)
                                                                                         aGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTAt                                                            <  1:1567633/150‑1 (MQ=255)
                                                                                             gTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGcg                                                        >  1:1983070/1‑150 (MQ=255)
                                                                                               aCCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGcgac                                                      >  1:1177576/1‑150 (MQ=255)
                                                                                                ccACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGcgacg                                                     >  1:2679824/1‑150 (MQ=255)
                                                                                                ccACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGcgacg                                                     >  1:3290914/1‑150 (MQ=255)
                                                                                                 cACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCgga                                                                                            >  2:1508063/1‑110 (MQ=255)
                                                                                                 cACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGcgacga                                                    >  2:1317762/1‑150 (MQ=255)
                                                                                                 cACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGcgacga                                                    >  2:4178021/1‑150 (MQ=255)
                                                                                                   cGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAgg                                                  >  1:531870/1‑150 (MQ=255)
                                                                                                      cATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTAc                                               >  2:2333031/1‑150 (MQ=255)
                                                                                                       aTGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACg                                              <  1:2118074/150‑1 (MQ=255)
                                                                                                            ggCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGtctc                                         >  2:5432937/1‑150 (MQ=255)
                                                                                                            ggCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGtctc                                         >  2:3533253/1‑150 (MQ=255)
                                                                                                              ccTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTcgc                                       >  1:2702247/1‑150 (MQ=255)
                                                                                                               cTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTcgcg                                      >  1:2016656/1‑150 (MQ=255)
                                                                                                                 gCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCa                                    <  2:2660956/150‑1 (MQ=255)
                                                                                                                    tCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGtt                                 >  2:3585136/1‑150 (MQ=255)
                                                                                                                    tCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGtt                                 >  2:3407092/1‑150 (MQ=255)
                                                                                                                     cAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGttt                                <  2:2969132/150‑1 (MQ=255)
                                                                                                                     cAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGttt                                <  1:688133/150‑1 (MQ=255)
                                                                                                                      aGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTg                               <  2:742918/150‑1 (MQ=255)
                                                                                                                       gTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGa                              <  1:2893255/150‑1 (MQ=255)
                                                                                                                        tGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAg                             <  2:3369188/150‑1 (MQ=255)
                                                                                                                         gCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGc                            <  2:1052685/150‑1 (MQ=255)
                                                                                                                           gggTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCAt                          >  2:2925686/1‑150 (MQ=255)
                                                                                                                           gggTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCAt                          >  2:2925325/1‑150 (MQ=255)
                                                                                                                           gggTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCAt                          >  2:5386688/1‑150 (MQ=255)
                                                                                                                                 cGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTcat                    >  2:4909167/1‑148 (MQ=255)
                                                                                                                                   gCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGa                  <  1:682881/150‑1 (MQ=255)
                                                                                                                                     cTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAg                >  1:5474428/1‑150 (MQ=255)
                                                                                                                                      tGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAAc                                                                       <  2:439837/94‑1 (MQ=255)
                                                                                                                                      tGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAAc                                                                       >  1:439837/1‑94 (MQ=255)
                                                                                                                                      tGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGt               <  2:4283380/150‑1 (MQ=255)
                                                                                                                                       gagcATGCTGCGCGGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGtt              >  2:2473786/4‑150 (MQ=255)
                                                                                                                                         gcATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGc            <  1:4796739/149‑1 (MQ=255)
                                                                                                                                           aTGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCtt          <  1:519946/150‑1 (MQ=255)
                                                                                                                                             gctgcCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCTTga        <  1:3540835/150‑1 (MQ=255)
                                                                                                                                             gctgcCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCTTga        <  2:2866234/150‑1 (MQ=255)
                                                                                                                                               tgcCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCTTgaag      >  1:4869132/1‑150 (MQ=255)
                                                                                                                                                gcCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCTTgaaga     >  1:3077272/1‑150 (MQ=255)
                                                                                                                                                   gcgcAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCTTGAAGAAgg  >  1:2271676/1‑150 (MQ=255)
                                                                                                                                                   gcgcAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCTTGAAGAAgg  >  1:2918213/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGCCTTCCGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCTTGAAGAAGG  >  NC_002947/3576521‑3576817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑