Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,773,267 C→A 5.2% P97Q (CCG→CAG)  PP_5600 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,773,2670CA 5.2% 782.1 / 22.3 229P97Q (CCG→CAG) PP_5600hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (118/99);  new base A (6/6);  total (124/105)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 233 total aligned reads displayed.

CACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCAGCGGGCGTGTGCTCAC  >  NC_002947/3773118‑3773415
                                                                                                                                                     |                                                                                                                                                     
 aCGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCg                                                                                                                                                      >  1:1324619/1‑150 (MQ=255)
 aCGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCg                                                                                                                                                      >  1:3967992/1‑150 (MQ=255)
   ggCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCggg                                                                                                                                                    >  2:5556861/1‑150 (MQ=255)
    gccgccAGCCGCGGCATGGTCCAAGGTGAGCACGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCCGCGCCACACTGCTGGCCGTCTTTTTCGGCGTGCTGACCCGGCTCGCCGGCGAGCAgggt                                                                                                                                                   <  2:5352270/150‑2 (MQ=255)
    gCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCGGCTCGCCGGCGAGCAgggt                                                                                                                                                   <  1:5322390/150‑2 (MQ=255)
       gACAGCAGCGGCAGGGTCCACGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACCCTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGc                                                                                                                                               <  1:2428804/150‑1 (MQ=255)
        aCAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTG‑CCCGGCTCGCCGGCGAGCAgggtcagca                                                                                                                                              <  1:3448656/150‑7 (MQ=255)
         cagcagCGGCATGGTCCAAGGTTAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCTTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGccc                                                                                                                                             <  1:1876922/150‑1 (MQ=255)
          agcagcGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGcccc                                                                                                                                            >  1:4485658/1‑150 (MQ=255)
            cagcGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCtt                                                                                                                                          >  1:4982791/1‑150 (MQ=255)
            cagcGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTGCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCtt                                                                                                                                          >  2:3906593/1‑150 (MQ=255)
              gcGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTAc                                                                                                                                        <  2:5451030/150‑1 (MQ=255)
                  cATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGTTCGCCGGCGAGCCGGG‑CAGCCCCTTACccaa                                                                                                                                    <  1:2960950/150‑1 (MQ=255)
                  cATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACccaa                                                                                                                                    >  2:5035520/1‑150 (MQ=255)
                   aTGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACccaac                                                                                                                                   >  1:4606001/1‑150 (MQ=255)
                   aTGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCATTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACccaac                                                                                                                                   <  2:374329/150‑1 (MQ=255)
                     ggTCCGAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACccaacca                                                                                                                                 >  1:285185/1‑150 (MQ=255)
                       tCCAAGGTGCGAAAGACTTCAGCGATCCGACCCTGCCTGCCGGCGGTGTCTGCGTGATCGCCAGCCTGTTCAGCGCCACCCTGCTGGCCGTCTTTTTCGGCCGGCTGACCCTGCTCGCGGGCGAGCCGGG‑CAGCCCCTTACCcaaccaac                                                                                                                               <  1:407349/150‑1 (MQ=255)
                       tCCAAGGTGATCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCcaaccaac                                                                                                                               <  1:2903062/150‑1 (MQ=255)
                       tCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCcaaccaac                                                                                                                               <  1:443402/150‑1 (MQ=255)
                       tCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCcaaccaac                                                                                                                               >  2:2738204/1‑150 (MQ=255)
                          aaGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCaaccacccgc                                                                                                                            >  2:4185503/1‑148 (MQ=255)
                          aaGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTc                                                                                                                            >  2:4370922/1‑150 (MQ=255)
                             gTGAGCAATACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGc                                                                                                                         <  1:3948783/150‑1 (MQ=255)
                             gTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGc                                                                                                                         <  1:3514765/150‑1 (MQ=255)
                             gTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGc                                                                                                                         >  1:1732609/1‑150 (MQ=255)
                               gAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CCGCCCCTTACCCAACCAACCTCCGCCa                                                                                                                       >  1:2404244/1‑150 (MQ=255)
                                  cAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGGTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGc                                                                                                                    <  1:129356/150‑1 (MQ=255)
                                  cAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGc                                                                                                                    >  1:708381/1‑150 (MQ=255)
                                      aCTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGc                                                                                                                <  2:4304697/150‑1 (MQ=255)
                                        ttCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGGCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGccc                                                                                                              >  2:4731895/1‑150 (MQ=255)
                                          cAGGGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTATTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCaa                                                                                                            <  1:4744372/150‑1 (MQ=255)
                                           aGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAag                                                                                                           >  1:5411499/1‑150 (MQ=255)
                                           aGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAag                                                                                                           >  1:2064979/1‑150 (MQ=255)
                                           aGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAag                                                                                                           >  2:1339069/1‑150 (MQ=255)
                                             cGATCCGACCCTGTCTGGGGGCGGTGACTGCTTGCTCGCCAGCCTGTTCAGCGCCACACTGCTGGCCTTCTTTTTCGGCGTGGTGACCCTGCTCGCCGGCGCGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAagca                                                                                                         <  1:4512526/150‑1 (MQ=255)
                                               aTCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCTTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAagcagc                                                                                                       <  2:4497201/150‑1 (MQ=255)
                                               aTCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAagcagc                                                                                                       <  1:433702/150‑1 (MQ=255)
                                                tCCGACCCTGTCTGCCGGCGGTGACTGCGTGTTCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAgcg                                                                                                      <  1:677329/150‑1 (MQ=255)
                                                tCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAgcg                                                                                                      <  1:1866339/150‑1 (MQ=255)
                                                 ccGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAgcgc                                                                                                     <  1:892659/150‑1 (MQ=255)
                                                  cGACCCTGTCTGCGGGCGGTGACGGCGTGATCGCCAGCCTGTTCAGCGCCACACTGGGGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCt                                                                                                    <  1:5329096/150‑1 (MQ=255)
                                                  cGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCGGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGCGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCt                                                                                                    <  1:2670727/150‑1 (MQ=255)
                                                   gACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTg                                                                                                   <  2:4106859/150‑1 (MQ=255)
                                                     cccTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCGGCTGACCCGGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGc                                                                                                 <  1:2556412/150‑1 (MQ=255)
                                                      ccTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGcc                                                                                                >  1:4253564/1‑150 (MQ=255)
                                                          tCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CCGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTg                                                                                            >  1:1579677/1‑150 (MQ=255)
                                                          tCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTg                                                                                            <  1:4214245/150‑1 (MQ=255)
                                                          tCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTg                                                                                            <  1:3855504/150‑1 (MQ=255)
                                                            tGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCACCCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGt                                                                                          >  2:3960486/1‑150 (MQ=255)
                                                            tGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGt                                                                                          >  2:2546872/1‑150 (MQ=255)
                                                              ccGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CCGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCa                                                                                        >  1:347544/1‑150 (MQ=255)
                                                              ccGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTC‑TTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCGTACCCAACCAACCTCCGCCCCGCCGGCCCAAGCGGCGCTGGCCACTGGTCaa                                                                                       >  1:1884403/1‑150 (MQ=255)
                                                                   ggTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CCGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATccc                                                                                   >  1:588299/1‑150 (MQ=255)
                                                                         tGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGcc                                                                             >  1:494166/1‑150 (MQ=255)
                                                                         tGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGcc                                                                             >  1:5580188/1‑150 (MQ=255)
                                                                              gATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCGTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTcc                                                                        >  2:77295/1‑150 (MQ=255)
                                                                               ttCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTccc                                                                       <  1:4997307/149‑1 (MQ=255)
                                                                               aTCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCGTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTccc                                                                       <  1:4759568/150‑1 (MQ=255)
                                                                                 cGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgc                                                                     >  1:2197123/1‑150 (MQ=255)
                                                                                   ccAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CCGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCACGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgcgt                                                                   >  2:337077/1‑148 (MQ=255)
                                                                                      gCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgcttcgc                                                                <  1:3747704/150‑1 (MQ=255)
                                                                                          gTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTtct                                                            <  2:1991922/150‑1 (MQ=255)
                                                                                           ttCAGCGCCACACTGTTGGCCGTCTTTTTGGGCATGCTGACCCGGCGCGCGGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTtctc                                                           <  1:2743853/150‑1 (MQ=255)
                                                                                           ttCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTtctc                                                           >  2:1156572/1‑150 (MQ=255)
                                                                                           ttCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCCCGCCTCTCCCGCTTCGCTgctc                                                           >  2:5097453/1‑150 (MQ=255)
                                                                                            tCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTcg                                                          >  2:708210/1‑150 (MQ=255)
                                                                                             cAGCGCCACCCTGCTGGCCGTCTTTTACGGCCTGCTGCCCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTCCCCACCCCACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCACTCCCCGCGCCTCTCCCGCTTCGCTTCTcgc                                                         <  2:3657924/150‑1 (MQ=255)
                                                                                              aGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTcgcg                                                        >  2:1576473/1‑150 (MQ=255)
                                                                                                cgcCACCCGGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCGCCCGCTTCGCTTCTCGCGtt                                                      <  2:73219/150‑1 (MQ=255)
                                                                                                 gcCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCGGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTc                                                     <  1:211605/150‑1 (MQ=255)
                                                                                                  ccACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCt                                                    <  1:5134979/150‑1 (MQ=255)
                                                                                                  ccACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCt                                                    >  1:3294449/1‑150 (MQ=255)
                                                                                                  ccACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCt                                                    <  2:2003324/150‑1 (MQ=255)
                                                                                                  ccACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCAGCGCCTCTCCCGCTTCGCTTCTCGCGTTCt                                                    >  1:1039640/1‑150 (MQ=255)
                                                                                                  ccACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGAGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCACGCTTCGCTTCTCGCGTTCt                                                    >  2:2144716/1‑150 (MQ=255)
                                                                                                      attgctgGCCGGCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCAACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTACTCGCGTTCTGACa                                                >  2:5583997/3‑150 (MQ=255)
                                                                                                      acTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCGCCCCAAGCAGCGCGCGCCACTCGTCAATCCCCGCGCCACTCCCGCGACGCTTCTCGCGTTCTGACa                                                >  2:2881222/1‑150 (MQ=255)
                                                                                                           tgGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGcc                                           <  2:5542949/150‑1 (MQ=255)
                                                                                                             gCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCAc                                         >  2:347356/1‑150 (MQ=255)
                                                                                                                gTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTc                                      >  1:4802014/1‑150 (MQ=255)
                                                                                                                   tttttCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTcccc                                   >  1:3670058/1‑150 (MQ=255)
                                                                                                                     tttCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGt                                 <  2:4976480/150‑1 (MQ=255)
                                                                                                                        cGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTgcc                              <  1:2328886/150‑1 (MQ=255)
                                                                                                                         ggCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CCGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCGTCGCTTCTCGCGGTCTGACATCGCCACGTCCCCGTgccg                             >  2:2319661/1‑150 (MQ=255)
                                                                                                                                ctgcccGGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATc                      >  1:4530488/4‑150 (MQ=255)
                                                                                                                                 tgcccGGCTCGCCGGCGAGCAGGGTCAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCAACTGGTCAATCCCCGCGCCTCTCCCGCTTAGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATc                      >  1:1453242/3‑150 (MQ=255)
                                                                                                                                 tgcccGGCTCGCCGGCGAGCAGGG‑TAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCg                     >  1:5576152/3‑150 (MQ=255)
                                                                                                                                 gACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCACCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCg                     >  1:1156483/1‑150 (MQ=255)
                                                                                                                                  aCCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCgg                    >  1:3137967/1‑150 (MQ=255)
                                                                                                                                  aCCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCgg                    >  2:4150883/1‑150 (MQ=255)
                                                                                                                                  aCCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCgg                    <  2:1017325/150‑1 (MQ=255)
                                                                                                                                    ccggCTCGCCGGCGAGCAGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCa                  >  2:4894808/4‑150 (MQ=255)
                                                                                                                                    ccTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCa                  >  1:3184174/1‑150 (MQ=255)
                                                                                                                                      tGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCAGc                >  2:1748307/1‑150 (MQ=255)
                                                                                                                                       gCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCAGCg               >  1:4119908/1‑150 (MQ=255)
                                                                                                                                          cGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCAGCGGGc            >  2:4389931/1‑150 (MQ=255)
                                                                                                                                            ccGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACAACGCCACGTCCCCGTGCCGCCTGATCGGCAGCGGGCgt          <  2:2816610/150‑1 (MQ=255)
                                                                                                                                               gCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCAGCGGGCGtgtg       <  2:4807542/150‑1 (MQ=255)
                                                                                                                                                 gAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCAGCGGGCGTGTGCt     >  2:2018772/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGCCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCAGCGGGCGTGTGCTCAC  >  NC_002947/3773118‑3773415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑