BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,876,599	G→C	5.7%	L259V (CTG→GTG)	PP_3420 ←	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,876,599	0	G	C	5.7%	824.5 / 29.7	244	L259V (CTG→GTG)	PP_3420	sensor histidine kinase
Reads supporting (aligned to +/- strand): ref base G (120/109); new base C (6/8); total (126/118)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 253 total aligned reads displayed.

CTTCTGCGCAGCAGCGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGTAGATTTCCACCATG  >  NC_002947/3876450‑3876747

cttctGCGCAGCAGCGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAg                                                                                                                                                      <  2:1980631/150‑1 (MQ=255)

 ttctGCGCAGCAGCGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAgg                                                                                                                                                     >  1:3051768/1‑150 (MQ=255)

 ttctGCGCAGCAGCGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAgg                                                                                                                                                     >  1:52399/1‑150 (MQ=255)

 ttctGCGCAGCAGCGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCAACCAGCCTGTCCTGCTGCAgg                                                                                                                                                     >  1:5409666/1‑150 (MQ=255)

  tctGCGCAGCAGCGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGt                                                                                                                                                    >  2:3311639/1‑150 (MQ=255)

    tGCGCAGCAGCGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTgc                                                                                                                                                  <  2:3685808/150‑1 (MQ=255)

     gcgcAGCAGCGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTgca                                                                                                                                                 >  1:1018750/1‑150 (MQ=255)

      cgcAGCAGCGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTgcag                                                                                                                                                >  2:4053257/1‑150 (MQ=255)

       gcagcaGCGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTgcagc                                                                                                                                               >  2:4366774/1‑150 (MQ=255)

        cagcagCGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCACCTgcagca                                                                                                                                              <  2:2260791/150‑1 (MQ=255)

         agcagcGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCat                                                                                                                                             >  1:2393761/1‑150 (MQ=255)

         agcagcGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCACCTgcagcag                                                                                                                                             <  2:1281676/150‑2 (MQ=255)

          gcagcGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCACCTgcagcagg                                                                                                                                            <  2:4082692/150‑3 (MQ=255)

           cagcGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCatat                                                                                                                                           <  1:5162759/150‑1 (MQ=255)

             gcGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATAtgt                                                                                                                                         <  2:3128141/150‑1 (MQ=255)

              cGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATAtgtg                                                                                                                                        <  1:5097812/150‑1 (MQ=255)

                ttGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTgcc                                                                                                                                      <  2:2784200/150‑1 (MQ=255)

                        ttCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGgcg                                                                                                                              <  1:763131/150‑1 (MQ=255)

                           cTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCAc                                                                                                                           <  2:4520027/150‑1 (MQ=255)

                           cTGGGCAAGTTCAGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCAc                                                                                                                           >  2:5572461/1‑150 (MQ=255)

                            tGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCata                                                                                                                          <  1:2808255/150‑3 (MQ=255)

                             gggCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGa                                                                                                                         >  1:14680/1‑150 (MQ=255)

                              ggCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGAt                                                                                                                        >  1:5221971/1‑150 (MQ=255)

                               gCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATc                                                                                                                       <  2:891254/150‑1 (MQ=255)

                                    ttCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCCGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGaa                                                                                                                  <  1:5200691/150‑1 (MQ=255)

                                     tCGGCGCTGGCGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAAc                                                                                                                 >  1:1777770/1‑150 (MQ=255)

                                      cGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACa                                                                                                                <  2:2060838/150‑1 (MQ=255)

                                      cGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACa                                                                                                                <  1:188912/150‑1 (MQ=255)

                                        gcgcTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAgg                                                                                                              >  1:2631688/1‑150 (MQ=255)

                                        gcgcTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAgg                                                                                                              <  2:5627541/150‑1 (MQ=255)

                                           cTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCg                                                                                                           <  1:3263857/150‑1 (MQ=255)

                                             ggAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGaa                                                                                                         <  1:2878095/150‑1 (MQ=255)

                                             ggAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGaa                                                                                                         >  1:2254278/1‑150 (MQ=255)

                                                gCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTa                                                                                                      <  1:5472274/150‑1 (MQ=255)

                                                gCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTa                                                                                                      >  2:3556070/1‑150 (MQ=255)

                                                gCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTa                                                                                                      >  2:2576629/1‑150 (MQ=255)

                                                  gTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGa                                                                                                    <  1:4651988/150‑1 (MQ=255)

                                                   tATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGCGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGAt                                                                                                   <  1:5547918/150‑1 (MQ=255)

                                                    aTGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGCAGATc                                                                                                  <  2:5386201/150‑1 (MQ=255)

                                                      gTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCaa                                                                                                >  2:5205365/1‑150 (MQ=255)

                                                       ttGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAAc                                                                                               <  2:2633440/150‑1 (MQ=255)

                                                           ccAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGccc                                                                                           <  1:1687365/150‑1 (MQ=255)

                                                             aGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCccca                                                                                         >  1:2065482/1‑150 (MQ=255)

                                                             aGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCccca                                                                                         >  2:3345435/1‑150 (MQ=255)

                                                                  gTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCccacccac                                                                                    >  1:2180073/1‑150 (MQ=255)

                                                                  gTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCccacccac                                                                                    <  1:1500925/150‑1 (MQ=255)

                                                                      cGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAgt                                                                                <  2:3406931/150‑1 (MQ=255)

                                                                            cTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAg                                                                          >  1:644896/1‑150 (MQ=255)

                                                                            cTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAg                                                                          <  1:1373760/150‑1 (MQ=255)

                                                                               tgAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCa                                                                                                                            <  1:810124/97‑1 (MQ=255)

                                                                               tgAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCa                                                                                                                            >  2:811941/1‑97 (MQ=255)

                                                                                gAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGccc                                                                      <  2:5016250/150‑1 (MQ=255)

                                                                                gAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGccc                                                                      >  1:3380412/1‑150 (MQ=255)

                                                                                  gCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAg                                                                    <  2:2929685/150‑1 (MQ=255)

                                                                                  gCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAg                                                                    >  1:3351175/1‑150 (MQ=255)

                                                                                   ccACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGa                                                                   <  1:3127832/150‑1 (MQ=255)

                                                                                   ccACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGa                                                                   >  2:2838971/1‑150 (MQ=255)

                                                                                        gCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGATGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGc                                                              <  2:4223333/150‑1 (MQ=255)

                                                                                        gCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGc                                                              <  2:3351175/150‑1 (MQ=255)

                                                                                         ccGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGca                                                             <  2:3995859/150‑1 (MQ=255)

                                                                                           gacgacATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGcaca                                                           >  2:5135210/1‑150 (MQ=255)

                                                                                           gacgacATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGcaca                                                           <  2:3800802/150‑1 (MQ=255)

                                                                                            acgacATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACAt                                                          >  1:4944134/1‑150 (MQ=255)

                                                                                            acgacATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACAt                                                          >  2:3763172/1‑150 (MQ=255)

                                                                                                 aTTTCACCCAGCGCAACGTAGGTTGCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCcg                                                     >  1:2027342/1‑150 (MQ=255)

                                                                                                  tttCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCcgc                                                    >  1:1287902/1‑150 (MQ=255)

                                                                                                  tttCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCAGATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCCGTGTCGAGGCCCAGATCAGCACATAGCcgc                                                    >  2:1542035/1‑150 (MQ=255)

                                                                                                       cccAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTgg                                               >  2:3457934/1‑150 (MQ=255)

                                                                                                       cccAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTgg                                               <  1:3421159/150‑1 (MQ=255)

                                                                                                       cccAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTgg                                               >  1:2516725/1‑150 (MQ=255)

                                                                                                        ccAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGGAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTggg                                              >  2:778688/1‑149 (MQ=255)

                                                                                                         cAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGAt                                             <  2:2997050/150‑1 (MQ=255)

                                                                                                          aGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATg                                            <  1:1949669/150‑1 (MQ=255)

                                                                                                             gcAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATgcgc                                         >  1:3790397/1‑150 (MQ=255)

                                                                                                             gcAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATgcgc                                         <  1:3094204/150‑1 (MQ=255)

                                                                                                              cAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTTCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGcc                                        <  2:2444293/150‑1 (MQ=255)

                                                                                                                aCGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCgg                                      >  2:1833851/1‑150 (MQ=255)

                                                                                                                   tAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATg                                   >  1:824228/1‑150 (MQ=255)

                                                                                                                     ggTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAg                                 <  2:5285979/150‑1 (MQ=255)

                                                                                                                     ggTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAg                                 <  2:2109033/150‑1 (MQ=255)

                                                                                                                      gTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCccca                                                                                         >  1:1100130/1‑93 (MQ=255)

                                                                                                                      gTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCccca                                                                                         <  2:1100122/93‑1 (MQ=255)

                                                                                                                      gTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAgg                                >  2:709357/1‑150 (MQ=255)

                                                                                                                      gTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAgg                                >  2:491102/1‑150 (MQ=255)

                                                                                                                           gCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTg                           <  1:4290410/150‑1 (MQ=255)

                                                                                                                            cTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGc                          >  1:3593081/1‑150 (MQ=255)

                                                                                                                            cTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGc                          <  1:4018381/150‑1 (MQ=255)

                                                                                                                             ttGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCg                         <  1:503593/150‑1 (MQ=255)

                                                                                                                                      gCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGt                <  1:5518739/150‑1 (MQ=255)

                                                                                                                                       ccTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGTa               <  1:3940121/150‑1 (MQ=255)

                                                                                                                                         tGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGa                                  >  1:5259422/1‑129 (MQ=255)

                                                                                                                                         tGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGTAGa             >  1:4803737/1‑150 (MQ=255)

                                                                                                                                           tCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGTAGAtt           >  1:2076445/1‑150 (MQ=255)

                                                                                                                                           tCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGTAGAtt           >  2:1681756/1‑150 (MQ=255)

                                                                                                                                             ctgctgCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGTAGATTTc         <  2:2973033/150‑1 (MQ=255)

                                                                                                                                              tgctgcAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGTAGATTTcc        >  1:650809/1‑150 (MQ=255)

                                                                                                                                              tgctgcAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGTAGATTTcc        >  2:2726671/1‑150 (MQ=255)

                                                                                                                                               gctgcAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGTAGATTTcca       >  1:372570/1‑150 (MQ=255)

                                                                                                                                               gctgcAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGTAGATTTcca       >  1:981416/1‑150 (MQ=255)

                                                                                                                                                    aGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGTAGATTTCCACCATg  >  1:2026703/1‑150 (MQ=255)

CTTCTGCGCAGCAGCGTTGGCGGTTTCCTGGGCAAGTTCGGCGCTGGAGCGTATGTTGGCCAGCGGGTTACGCATGCTGTGAGCCACTGCCGACGACATTTCACCCAGCGCAACGTAGGTTTCGCTTGCCACCAGCCTGTCCTGCTGCAGGTGCAGCATATGTGCCGCCCGGCGCACGATCCAGAACAGGCCGAAGTAGATCAACGCCCCACCCACCAGTGTCGAGGCCCAGATCAGCACATAGCCGCGCTGGATGCGCCGGATGAGGTCTTGCGGCTCCTTGTAGATTTCCACCATG  >  NC_002947/3876450‑3876747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`