Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,163,732 A→G N304D (AAC→GAC)  gtsA → mannose/glucose ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,163,7320AG99.5% 773.5 / NA 218N304D (AAC→GAC) gtsAmannose/glucose ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (93/124);  minor base T (1/0);  total (94/124)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

TGGAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTT  >  NC_002947/1163586‑1163878
                                                                                                                                                  |                                                                                                                                                  
tGGAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCAGGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTacgaca                                                                                                                                                 >  2:3019362/1‑150 (MQ=255)
 ggAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACAt                                                                                                                                                >  1:1038079/1‑150 (MQ=255)
  gAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATc                                                                                                                                               <  1:3089111/150‑1 (MQ=255)
  gAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATc                                                                                                                                               <  1:5486629/150‑1 (MQ=255)
  gAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCAGTACTGACAAGTCCTTCCTGTACGACATc                                                                                                                                               <  1:4510698/150‑1 (MQ=255)
       cgccAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTc                                                                                                                                          <  1:4434920/150‑1 (MQ=255)
          cAAGGTCATTAACGTCAAGGCCGGCATTCAGATCATGGGCGACTGGGCCAAGAGAGAATGGACGCTGGCGAAGAAAACTTCTGGCAAGGATTACCAGTGCGTGCCTTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGtt                                                                                                                                       <  1:4339140/150‑1 (MQ=255)
           aaGGTCATTAACGGCAAGGCCGGCATGCAGTTCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTtg                                                                                                                                      <  1:4854556/150‑1 (MQ=255)
           aaGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTtg                                                                                                                                      <  2:4185512/150‑1 (MQ=255)
           aaGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACCGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTtg                                                                                                                                      >  1:5822335/1‑150 (MQ=255)
              gTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTtggtg                                                                                                                                   <  1:1549839/150‑1 (MQ=255)
              gTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTtggtg                                                                                                                                   <  2:4801287/150‑1 (MQ=255)
              gTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTtggtg                                                                                                                                   <  1:3199546/150‑1 (MQ=255)
                cATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTggtggt                                                                                                                                 >  1:1927921/1‑150 (MQ=255)
                cATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTggtggt                                                                                                                                 <  1:2512502/150‑1 (MQ=255)
                  ttAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGgtgt                                                                                                                               >  2:3311553/1‑150 (MQ=255)
                   tAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGtt                                                                                                                              >  2:1642803/1‑150 (MQ=255)
                    aaCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTc                                                                                                                             <  1:5652639/150‑1 (MQ=255)
                     aCGGCAAGTCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCa                                                                                                                            >  1:1135457/1‑150 (MQ=255)
                     aCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCa                                                                                                                            >  2:2512446/1‑150 (MQ=255)
                        gCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGc                                                                                                                         >  1:2332062/1‑150 (MQ=255)
                        gCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGc                                                                                                                         <  1:2213255/150‑1 (MQ=255)
                           aGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGa                                                                                                                      <  1:5042108/150‑1 (MQ=255)
                            ggCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGaa                                                                                                                     <  2:2607120/150‑1 (MQ=255)
                                 gCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGATACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACg                                                                                                                >  2:1164666/1‑150 (MQ=255)
                                   aTGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCt                                                                                                              <  1:2587939/150‑1 (MQ=255)
                                     gCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTgg                                                                                                            <  1:2057083/150‑1 (MQ=255)
                                       aGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCa                                                                                                          >  1:4241925/1‑150 (MQ=255)
                                       aGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCa                                                                                                          <  2:5645996/150‑1 (MQ=255)
                                         aTCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCAcc                                                                                                        >  2:1815895/1‑150 (MQ=255)
                                             tGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATc                                                                                                                                               >  2:4420602/1‑107 (MQ=255)
                                              gggCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgc                                                                                                   <  1:3901378/150‑1 (MQ=255)
                                                gCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgctg                                                                                                 <  2:495069/150‑1 (MQ=255)
                                                gCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgctg                                                                                                 >  2:2922113/1‑150 (MQ=255)
                                                  gACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGt                                                                                               >  1:679610/1‑150 (MQ=255)
                                                  gACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGt                                                                                               >  2:3401923/1‑150 (MQ=255)
                                                   aCTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTc                                                                                              <  1:4369261/150‑1 (MQ=255)
                                                    cTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTca                                                                                             <  1:4738310/150‑1 (MQ=255)
                                                          cAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGa                                                                                       <  2:5822335/150‑1 (MQ=255)
                                                                 gAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGcc                                                                                >  1:2138917/1‑150 (MQ=255)
                                                                       aCCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACCACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAg                                                                          >  2:3951779/1‑150 (MQ=255)
                                                                       aCCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAg                                                                          >  1:4785098/1‑150 (MQ=255)
                                                                         ccTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGgt                                                                        >  2:3075175/1‑150 (MQ=255)
                                                                            ggCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCACGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTTGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCATCAGGACATCGCCCGCAATTTGtt                                                                     <  2:3327829/150‑1 (MQ=255)
                                                                             gCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTg                                                                    <  2:1519912/150‑1 (MQ=255)
                                                                             gCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTg                                                                    <  2:1727004/150‑1 (MQ=255)
                                                                             gCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTg                                                                    <  2:5002978/150‑1 (MQ=255)
                                                                              cGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTgg                                                                   <  1:1761008/150‑1 (MQ=255)
                                                                              cGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTgg                                                                   <  1:466924/150‑1 (MQ=255)
                                                                               gaagaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTggg                                                                  >  1:3302998/1‑150 (MQ=255)
                                                                                   aaaaCTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAg                                                              <  2:2168947/150‑1 (MQ=255)
                                                                                              cAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCTACTCTTTGGTGGTGTTCTAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCATGACATCGCCCGCAAGGTGTTGTTTGAGGACTTCCAGaa                                                   <  2:4874081/150‑1 (MQ=255)
                                                                                              cAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGaa                                                   <  1:5308401/150‑1 (MQ=255)
                                                                                               aaGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAg                                                  >  1:4830143/1‑150 (MQ=255)
                                                                                               aaGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAg                                                  <  2:1023717/150‑1 (MQ=255)
                                                                                                 ggATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGt                                                <  1:4092477/150‑1 (MQ=255)
                                                                                                  gtttACCAGTGCGTGCCGTTCCCCGGTACGGACACGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTc                                               <  2:5387652/148‑1 (MQ=255)
                                                                                                  gATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGATGGCACCTCTGCTGGTCAGCAGGACATCGACCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTc                                               >  2:447693/1‑150 (MQ=255)
                                                                                                   aTTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGTCTTCCAGAAGGTCt                                              <  1:62118/150‑1 (MQ=255)
                                                                                                   aTTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCt                                              >  1:4885457/1‑150 (MQ=255)
                                                                                                     tACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTc                                            >  2:4038612/1‑150 (MQ=255)
                                                                                                      aCCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCa                                           <  1:2922113/150‑1 (MQ=255)
                                                                                                      aCCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCa                                           <  1:443063/150‑1 (MQ=255)
                                                                                                       ccAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAg                                          >  2:4237677/1‑150 (MQ=255)
                                                                                                        cAGTGCGTGCCGTTCCCCGGTACTGGCAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGc                                         <  2:606969/150‑1 (MQ=255)
                                                                                                        cAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATc                                                                                   >  1:4996270/1‑108 (MQ=255)
                                                                                                        cAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGTGGACTTCCAGAAGGTCTTCAGc                                         >  2:298503/1‑150 (MQ=255)
                                                                                                        cAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGc                                         >  1:1484540/1‑150 (MQ=255)
                                                                                                         aGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCTGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCa                                        <  1:2841105/150‑1 (MQ=255)
                                                                                                          gtgcgtgcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAg                                                  <  2:2383692/139‑1 (MQ=255)
                                                                                                          gtgcgtgcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAg                                                  >  1:2383692/1‑139 (MQ=255)
                                                                                                          gtgcgtgcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCAt                                       >  2:463647/1‑150 (MQ=255)
                                                                                                            gcgtgcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATca                                     >  1:4409871/1‑150 (MQ=255)
                                                                                                             cgtgcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATcaa                                    <  1:4179603/150‑1 (MQ=255)
                                                                                                              gtgcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATcaac                                   <  2:701739/150‑1 (MQ=255)
                                                                                                                gcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATcaacaa                                 <  1:5598534/150‑1 (MQ=255)
                                                                                                                 ccGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAg                                >  2:4277831/1‑150 (MQ=255)
                                                                                                                 ccGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAg                                >  2:2557330/1‑150 (MQ=255)
                                                                                                                  cGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAgg                               <  1:2624847/150‑1 (MQ=255)
                                                                                                                      ccccGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTc                           <  1:1994687/150‑1 (MQ=255)
                                                                                                                        ccGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGa                         >  1:4602445/1‑150 (MQ=255)
                                                                                                                        ccGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGa                         >  1:2755229/1‑150 (MQ=255)
                                                                                                                        ccGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGa                         >  2:4412586/1‑150 (MQ=255)
                                                                                                                        ccGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGa                         <  2:4850270/150‑1 (MQ=255)
                                                                                                                           gTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATcc                      >  1:5321656/1‑150 (MQ=255)
                                                                                                                           ctACTGACAACACCTTCCTGTACGACATCGACTCGATGGTGGAGTTCAAGCACAACAACGCTGGCACCACTGCTGGACAGCAGCACATCGCCCGCAAGGTGTTGGGTGAGGACTACCAGAAGGTCTTCAGCATCAACAAGGGTTCGATcc                      <  2:3681373/149‑1 (MQ=255)
                                                                                                                             aCTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCg                    <  1:54593/150‑1 (MQ=255)
                                                                                                                              cTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCgg                   >  2:3752837/1‑150 (MQ=255)
                                                                                                                              cTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCgg                   >  1:3317177/1‑150 (MQ=255)
                                                                                                                                 aCAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAgg                                                                         >  2:3171770/1‑93 (MQ=255)
                                                                                                                                 aCAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTAc                >  1:2684082/1‑150 (MQ=255)
                                                                                                                                 aCAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTAc                >  1:3124616/1‑150 (MQ=255)
                                                                                                                                      tccttcctGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAAc           >  1:700882/1‑150 (MQ=255)
                                                                                                                                      tccttcctGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAAc           >  2:1335547/1‑150 (MQ=255)
                                                                                                                                      tccttcctGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAAc           >  1:1955469/1‑150 (MQ=255)
                                                                                                                                         ttcctGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGAc        >  1:85119/1‑150 (MQ=255)
                                                                                                                                         ttcctGTACGACATCGACTCATTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGAc        >  2:4308662/1‑150 (MQ=255)
                                                                                                                                             tGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGc    >  1:1364963/1‑150 (MQ=255)
                                                                                                                                             tGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCGGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGc    <  2:2763920/150‑1 (MQ=255)
                                                                                                                                               tACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCtt  >  2:2074288/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGGAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTT  >  NC_002947/1163586‑1163878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑