Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,148,7930AC53.3% ‑5.8 / 17.1 31V153G (GTC→GGC) PP_1007transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base A (11/3);  new base C (15/1);  total (27/4)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.47e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑ACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTT  >  NC_002947/1148658‑1148922
                                                                                                                                        |                                                                                                                                 
gggACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑a                                                                                                                                   <  2:753359/136‑1 (MQ=255)
     gtgtaaGTACGCCGCGCCGTGTAGCCGGGGGGCGAGCTGGCGGCCGTCCGGGGGTTCGCTGGCTATTGCCTATTTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCCGGCGCTGGCGCTCGCCG‑accacc                                                                                                                              <  2:598866/132‑1 (MQ=255)
          aGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGTGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑accacccgaag                                                                                                                         >  2:1593307/1‑131 (MQ=255)
          aGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGGCG‑gccccccggca                                                                                                                         >  1:598866/1‑131 (MQ=255)
          aGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑cccccccgaat                                                                                                                         >  1:563427/1‑131 (MQ=255)
          aGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCCCCGGaaa                                                                                                                         >  1:2425590/1‑135 (MQ=255)
                 cTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCCCCGGAAGGTGGTCg                                                                                                                  >  2:2643843/1‑136 (MQ=255)
                 cTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCACCGTAAGGTGGTCg                                                                                                                  >  2:212936/1‑136 (MQ=255)
                 cTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCACCGTAAGGTGGGCg                                                                                                                  >  2:2434101/1‑136 (MQ=255)
                  tCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCCCCCGAATGTGGTggg                                                                                                                 >  1:710766/1‑136 (MQ=255)
                  tCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑ACCACCGTAAGGTGGTCgg                                                                                                                 >  1:2142286/1‑136 (MQ=255)
                      ccTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCCCCGTAAGGTGGTCGGCCTg                                                                                                             >  2:1598730/1‑136 (MQ=255)
                      ccTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCCCCGGAAGGTGGTGGGccgg                                                                                                             >  1:2768213/1‑134 (MQ=255)
                      ccTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCCCCGGAAGGTGGTGGGCCTg                                                                                                             >  2:2734375/1‑136 (MQ=255)
                      ccTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCAGAAATGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑ACCCCCGGTAGGTGGGAGGccag                                                                                                             >  2:2973617/1‑134 (MQ=255)
                        tGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCCCCGGAAGGTGGTggggcaggc                                                                                                           >  1:1746937/1‑130 (MQ=255)
                             cAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCCCCGTTAGGTGGTCGGCCTGCAGgcgc                                                                                                      >  2:712185/1‑136 (MQ=255)
                             cAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑ACCCCCGGAAGGTGGGCGGGCGGCAGgcgc                                                                                                      >  2:31868/1‑136 (MQ=255)
                             cAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑ACCACCGTAAGGTGGTCGGCCTGCAGgcgc                                                                                                      >  2:2113946/1‑136 (MQ=255)
                             cAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCC‑GCCCCCCGGAGGGGGGGGGCCCGCAGgcgg                                                                                                      >  1:2224442/1‑135 (MQ=255)
                               ggcgggcgACCGGGCGGCGGTCCGGGCGTTCGCGGGCTACTGCCGAATTGGTGTTGCGCAGCACTTTTGCGCCGTCTTCCTGTTGCTGGCGCGGGCGCTCGCCG‑ACCGCCGTAAGGTGGTCGGCCTGCAGGCGCAt                                                                                                    <  1:1276678/136‑1 (MQ=255)
                                  gggcgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGCCCCACCG‑AAGGTGGTGGGGCACCAGgcgccaggg                                                                                                 >  1:1569730/1‑131 (MQ=255)
                                  gggcgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑ACCACCGTAAGGTGGTCGGCCTGCAGGCGCATggg                                                                                                 >  2:1760813/1‑136 (MQ=255)
                                   ggcgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCCCCGGAAGGTGGTCGGCCGGCCGGCGCCTgggg                                                                                                >  1:756792/1‑135 (MQ=255)
                                     cgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑ACCACCGGAATGGGGGGGGCCGGCAGGCGCAAGGGCAg                                                                                              >  2:9978/1‑136 (MQ=255)
                                       aCCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCCCCCGAAGGTGGGGGGCCGGGCGGCGCCTgggggggg                                                                                            >  1:1968377/1‑131 (MQ=255)
                                       aCCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑ACCCCCGTTAGGTGGTGGGGCTGCAGGGGCATGGGGAggg                                                                                            >  1:1509168/1‑135 (MQ=255)
                                              ggcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCCCCGGAAGGTGGTCGGCCGGCAGGCGCATGGGGAGGTTTACGAc                                                                                     >  1:1756198/1‑136 (MQ=255)
                                                                        ccctaaTGGGTGTTCCCCACCACTTTGGCCCCGTCTACCTGTGCCCGGGGGGGGCGCCCGCCC‑CCCCCCTTATGGTGGTCGCCCTGCAGGCGCGTGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAgcg                                                           <  1:1192890/132‑1 (MQ=255)
                                                                               tGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑ACCCCCGTAAGGTGGTCGGCCAGCAGGCGCAAGGGCCGGTTACCGACGGGGAATAGCCCCACCCCCAGCAGCGCTGCggg                                                    >  1:933708/1‑135 (MQ=255)
                                                                                               ttttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑ACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTGCAg                                    >  1:883507/1‑136 (MQ=255)
                                                                                               ttttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑ACCACCCGAAAGTGGTCGGCCGGCAGGCGCATGGGCCGGTTTCCGACGGGGTATATCCCCCCCCGCCGCAGCGCGGGGGGGGCCAGGGGGTTGCAg                                    >  1:1102119/1‑136 (MQ=255)
                                                                                                         tCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑CCCCCCGGAAGGTGGTCGGCCGGCCGGCGCCTGGGGCGGTTTCCGCCGGGGGATAGCCCGACCCGCCGCCGCGCTGCGGTGGGCCGGGGGTTGCAGTGTCAgcgcc                          >  1:1718028/1‑135 (MQ=255)
                                                                                                             cctgTTGCCGGCGCTGCCGCTCTCCC‑ACCCCCGTTCGGTGTGCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgcg                      <  2:1226804/133‑1 (MQ=255)
                                                                                                              cAGTTGCAGGCGCTGGCGCTCGCCG‑ACCACCGGAAGGTGGGCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATATCCCGGCCAGCCGCAGCGGTGCGGGGGCCAGGGGGTTACAGTGAATGCGCAGgcgc                     >  1:986021/1‑136 (MQ=255)
                                                                                                                  gcccggcgcggccgccCCCCC‑ACCCCCGTCCGGGGGGCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCcgc                 <  1:2113946/132‑1 (MQ=255)
                                                                                                                                  ccccccacccccTTAGGGGGGGCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGtt  <  1:1617901/130‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
GGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCG‑ACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTT  >  NC_002947/1148658‑1148922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑