| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,544,446 | 0 | T | G | 47.2% | 28.1 / 11.3 | 36 | V403G (GTG→GGG) | mdfA | multidrug MFS transporter |
| Reads supporting (aligned to +/- strand): ref base T (12/7); new base G (0/17); total (12/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTA > NC_002947/1544315‑1544550 | gatCAGGATGGCGTTGGTGGGGTTTGGCGCAGGTGTGCTGGCGATGATTGGCGGCGGTGCCTGGTTGCTGCATTTTGCCG‑TGGGGGTGGGGGGGGGGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTTTGGTTTGTggtgggt < 2:2867155/136‑1 (MQ=255) cACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGtgggtggg > 1:1163630/1‑136 (MQ=255) cACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGtgggtggg > 2:2759862/1‑136 (MQ=255) cACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGtgggtggg > 2:209915/1‑136 (MQ=255) aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGTCGATGATTGGCGACGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCAGCTGTGGTTTGTGGTGGGTGggc > 1:1591724/1‑136 (MQ=255) aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGggc > 1:63509/1‑136 (MQ=255) aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGggc > 1:1204741/1‑136 (MQ=255) ttGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGGGCGGcc > 1:1327410/1‑136 (MQ=255) ttGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGGGCGGcc > 1:2658771/1‑136 (MQ=255) gggTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAgggg > 1:2443290/1‑136 (MQ=255) gggTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAgggg > 2:3150985/1‑136 (MQ=255) ggCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAg > 1:2463826/1‑136 (MQ=255) cGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCt > 1:2230964/1‑136 (MQ=255) cGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCt > 2:2469294/1‑136 (MQ=255) atgatTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGc > 2:549521/1‑136 (MQ=255) gcccAGTTTGCT‑CATTTTCCCCGGGGGGGGGGGGGGGGGGGGGGC‑GTG‑GGCGCTGT‑GGCCGCGGTGTTTTGTGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCctct < 2:2743860/134‑1 (MQ=255) cggCTTGCTGAATTGGGCCC‑GGGGGGGGGGGGGGGGGGGGGC‑GTG‑GGCGGTGT‑GGCCGGTGGGGTTGGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTcg < 2:2962100/134‑1 (MQ=255) ttgcTTTTTGCCG‑GGGGGGGGGGGGGGGGGGGGGC‑GTG‑GGCGGTGG‑GGCCGCTGTGGTTTGTGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 1:16759/135‑1 (MQ=255) ctgcTTTTGGCCC‑TGGGGGGGGGGGGGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 2:1836872/136‑1 (MQ=255) gtgtttttGCCCGGGGGGGGGGGGGGGGGGGGGGC‑GTG‑GGCGCTGG‑GGCCGCGGTGGTTTGTGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 1:2787623/132‑1 (MQ=255) gtgaTTTTGCCCGGGGGGGGGGGGGGGGGGGGGGC‑GTG‑GGCGCTGG‑GGCCGCGGTGGTTGGTGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 2:1211259/133‑1 (MQ=255) tttGGCC‑GGGGGGGGGGGGGGGGGGGGGG‑GTTGGGCGTGGT‑GGCGGGTGGGGTTTGGGGGGGGTGGGCGGCCGGGGGATGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCg < 1:2400308/136‑1 (MQ=255) gggggggggCGGGGGCGGTG‑GGCGGGGG‑GGCCGCGGTGGTTGGGGGGGGGGGGGCGGCCGGGGCAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAggg < 1:913098/135‑1 (MQ=25) gggggggggCGGGGGC‑CGG‑GGGGCCGGGGGCCGCGGGGGTTGGGGGGGGGGGGGCGGCGGGGGTAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAggg < 1:930013/135‑1 (MQ=19) ggggggggggggggg‑gTG‑GGGGCGGG‑GGCGGGGGGGGTTGGTGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgcc < 1:3013920/136‑1 (MQ=25) ggggggggggggggC‑TTG‑GGCGCGGT‑GGCCGCTGTGGTTTGGGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgcc < 1:2639721/136‑1 (MQ=38) ggggggggggggggC‑GTT‑GGGGCGGT‑GGCGGGGGTGGTTTGGGGTGGGTGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgcc < 1:1280579/136‑1 (MQ=37) gggggggggggggg‑GTG‑GGGGGGGT‑GGGGGTTGTGGTTTTTGGGGGGGGGGGGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccg < 1:372130/136‑1 (MQ=25) gggggggggggggg‑GTG‑GGCGCGGG‑GGCGGGGGGGGTGGGGGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccg < 1:453467/136‑1 (MQ=25) ggggggggggggGC‑GTT‑GGCGCGGT‑GGCCGCTGTGGTTTGGGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccg < 2:161856/136‑1 (MQ=39) ggggggggggggGC‑GTG‑GGCGCGGG‑GGCCGCTGGGGTTGGGGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccg < 1:209915/136‑1 (MQ=37) gggggggggggc‑ggg‑ggggcggT‑GGCGGGGGGGGTTGGGGGGGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccgcg < 2:2483061/130‑1 (MQ=25) gggggCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccgcg < 1:2838895/133‑1 (MQ=255) gggggggggC‑GTT‑GGCGCTGT‑GGCCGCGGTGGTTTGTGGGGGGGGGGGGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccgcgcc < 1:819690/132‑1 (MQ=39) gggggg‑GTG‑GGCGTTGT‑GGCCGGTGGGGTGTGGGTGGGGGGGGGGGCCTGGGGAAGGGGCTGTAGCTCGATATTCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACTCAGGGCCGCGCCGCtt < 2:1797061/136‑1 (MQ=25) gC‑GTG‑GGGGCGGG‑GGCCGCGGTGGTTGGGGGGGGGTGGGGGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTTTTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTa < 2:2802830/136‑1 (MQ=37) gggTT‑GGCGCTGG‑GGCCGCTGGGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTa < 1:2243226/134‑1 (MQ=255) gggtgggCGCGGT‑GGCGGGTGTGGTTGGTGGTGGGGGGGCGGCCTGGGTAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTa < 1:986268/131‑1 (MQ=38) gCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa > 2:113246/1‑86 (MQ=255) gCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 1:113246/86‑1 (MQ=255) ggtgggtgGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa > 1:603741/1‑74 (MQ=255) ggtgggtgGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 2:603741/74‑1 (MQ=255) | GATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGC‑GTT‑GGCGCTGT‑GGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTA > NC_002947/1544315‑1544550 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |