BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,392,927:1	+A	15.4%	intergenic (+91/‑95)	PP_1214 → / → ruvC	transcriptional regulator/crossover junction endodeoxyribonuclease RuvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,392,927	1	.	A	15.4%	508.8 / 96.5	136	intergenic (+91/‑95)	PP_1214/ruvC	transcriptional regulator/crossover junction endodeoxyribonuclease RuvC
Reads supporting (aligned to +/- strand): ref base . (53/62); new base A (8/13); total (61/75)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 142 total aligned reads displayed.

TGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGG  >  NC_002947/1392778‑1393076

tGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑cc                                                                                                                                                         <  1:989890/150‑1 (MQ=255)

 gCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑cc                                                                                                                                                         >  1:1354501/1‑149 (MQ=255)

 gCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑cc                                                                                                                                                         <  2:1354501/149‑1 (MQ=255)

   aGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CAt                                                                                                                                                     <  1:4328799/150‑1 (MQ=255)

    gAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CAtt                                                                                                                                                    <  2:4096019/150‑1 (MQ=255)

         tCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCaaa                                                                                                                                               <  2:3274142/150‑1 (MQ=255)

         tCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCaaa                                                                                                                                               <  2:3778592/150‑1 (MQ=255)

          cTATTCCAATGCCCAGCTTGCCGCCGAGAACTAGGAAAACCTCCGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGAGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GTGTAAA‑CCCGCTCCTACAGGTAGTACG‑CC‑CCTTTCAAAt                                                                                                                                              <  1:1634907/150‑1 (MQ=255)

           tATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAAtg                                                                                                                                             <  2:1439770/150‑1 (MQ=255)

             ttCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAAtgtg                                                                                                                                           <  1:4190189/150‑1 (MQ=255)

             ttCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAAtgtg                                                                                                                                           <  1:2868141/150‑1 (MQ=255)

             ttCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAAtgtg                                                                                                                                           <  2:4193509/150‑1 (MQ=255)

             ttCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAAtgtg                                                                                                                                           <  1:3560335/150‑1 (MQ=255)

               ccAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggt                                                                                                                                         <  1:1585910/150‑1 (MQ=255)

               ccAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggt                                                                                                                                         <  2:896129/150‑1 (MQ=255)

                cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggtg                                                                                                                                        <  2:4363537/150‑1 (MQ=255)

                cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggtg                                                                                                                                        <  2:1885807/150‑1 (MQ=255)

                cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCGAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggtg                                                                                                                                        <  2:124308/150‑1 (MQ=255)

                    gCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGt                                                                                                                                    <  1:3484319/150‑1 (MQ=255)

                     cccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTa                                                                                                                                   >  2:3832984/1‑150 (MQ=255)

                      ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTAc                                                                                                                                  <  2:1774636/150‑1 (MQ=255)

                      ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTAc                                                                                                                                  <  2:2368626/150‑1 (MQ=255)

                      ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTAc                                                                                                                                  <  2:2368662/150‑1 (MQ=255)

                        aGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCt                                                                                                                                <  2:2217255/150‑1 (MQ=255)

                        aGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCt                                                                                                                                <  2:4399851/150‑1 (MQ=255)

                         gATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTg                                                                                                                               <  1:2998880/150‑1 (MQ=255)

                          aTTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGt                                                                                                                              <  2:1016199/150‑1 (MQ=21)

                            ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGTTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                            <  1:1103546/150‑1 (MQ=255)

                            ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                            <  2:3786896/150‑1 (MQ=255)

                            ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                            <  1:3281105/150‑1 (MQ=255)

                             tCCGCCGAGATCATGGAACACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                           <  1:1916314/150‑1 (MQ=18)

                             tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                           <  1:1916310/150‑1 (MQ=18)

                              ccGACGAGATCATGGAAACCCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGa                                                                                                                          <  1:285505/150‑1 (MQ=17)

                              ccGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGa                                                                                                                          <  1:361716/150‑1 (MQ=255)

                               cgacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAg                                                                                                                         <  2:2454211/150‑1 (MQ=18)

                                gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                        <  1:973625/150‑1 (MQ=255)

                                gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                        <  1:4128711/150‑1 (MQ=255)

                                 acgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑g                                                                                                                      <  2:303805/150‑1 (MQ=255)

                                  cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                     <  2:4160555/150‑1 (MQ=255)

                                  cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                     <  1:372196/150‑1 (MQ=255)

                                  cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                     <  1:2688420/150‑1 (MQ=255)

                                  cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑gg                                                                                                                     <  2:3769324/150‑1 (MQ=17)

                                   gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑ggg                                                                                                                    <  2:4002427/150‑1 (MQ=255)

                                   gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑ggg                                                                                                                    <  2:3629026/150‑1 (MQ=255)

                                    agaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGt                                                                                                                   <  2:4355879/150‑1 (MQ=18)

                                     gaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCCCGCCAACACGTATTAAG‑CC‑CCAATAAAAAGTGGTGCAGGACCGGGAGGGGC‑ggggt                                                                                                                  >  2:1907814/1‑148 (MQ=255)

                                     gaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCATTCGC‑GGGTAAA‑CCCCCGCCTACAGGTATTACG‑CC‑CAATTAAAAAGTGGTGCAGTACCAGGAGGGGC‑ggggt                                                                                                                  >  2:1907894/1‑148 (MQ=255)

                                      aTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGATTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGttt                                                                                                                 <  1:2024226/150‑1 (MQ=255)

                                        cATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTAc                                                                                                               <  1:490568/150‑1 (MQ=255)

                                         aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CAATTGAAATGTTGTGGCGTACCTGTAGGAGC‑GGGTTTAcc                                                                                                              >  2:2667504/1‑150 (MQ=38)

                                         aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCGTTCGC‑GGGTAAA‑ACCCCACCGACAGGTATTACG‑CC‑CAATTAAAATGTGGTGGCGTACCGGTGGGAGG‑GGGTTTaac                                                                                                              >  2:2667444/1‑148 (MQ=255)

                                           ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTTAA‑ACCCCGCCGACAGGGATTAAG‑CC‑CATTTGAAATGTGGGGGAGTAACGGTGTGAGCGGGGTTTAccg                                                                                                             >  2:1554695/1‑149 (MQ=255)

                                           ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCcg                                                                                                            <  1:3052465/150‑1 (MQ=17)

                                           ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CACCCTCCAACAGGTATTAAGCCC‑CATTACAAA‑GGGGGGCCGTACCAGTAGGAGC‑GGGTTTAACcg                                                                                                            >  2:1554687/1‑150 (MQ=37)

                                           ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGGAAA‑CCCGCGCCGACAGGTATTACG‑CC‑CCTTTCCAATTTGGTGGAGTACCGGGTGGTGC‑GGGTTTagacg                                                                                                            >  1:2218242/1‑146 (MQ=255)

                                            gAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCcgc                                                                                                           <  1:2732012/150‑1 (MQ=255)

                                            gAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCcgc                                                                                                           <  2:3196375/150‑1 (MQ=17)

                                                 ccTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑ACCGCGCCAACAGGTATTACG‑CC‑CATTTCAAATGTGGGGCAGTACCGGTAGGAGC‑GGGTTTACCCGCGAAAg                                                                                                      >  2:711280/1‑150 (MQ=38)

                                                   tCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCCCAACAACAGGTATTACG‑CC‑CCATTCAAATGGGGTGCCGTACCGGTAGGGGC‑GGGTTGAACCGCGCAAggg                                                                                                    >  2:172372/1‑150 (MQ=255)

                                                      gCTATGGTGATTGGGAATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCGGTGCGGGCCCTTTCGC‑GGGTAAC‑CCCGCTCCTACAGGTATTACG‑CC‑AATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGTTTTACCCGCGAAAGGGCCg                                                                                                 <  2:2154507/150‑1 (MQ=37)

                                                      gCTAAGGTGATTTGGCAGTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGGTAAA‑CCC‑CACCGACCGGGATTAAG‑CC‑CCATTCAAATGTGGTGCGCTACCGGGTGGAGG‑GGGGTTGCCCCCGACAAGGCCg                                                                                                 >  2:3779046/1‑150 (MQ=255)

                                                                            tgCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCCCTCCAACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCCCGAAAGGGCCGGCGCAGGTTGTAGAGATGcaac                                                                           >  2:4033761/1‑148 (MQ=255)

                                                                            tgCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑ACCGCTACAACAGGTATTAAG‑CC‑CATTTAAAATGTTGTGGAGTACATGTAGGAGC‑GGGTTTAACCGCGAAAGGGCCGGCGCAGGTTGTAGAGATTcaaa                                                                           >  2:238531/1‑148 (MQ=25)

                                                                              ccAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                            >  2:291253/1‑100 (MQ=255)

                                                                                aaCTCTGTGCGTTGGATAGCGCGTGGGCCGCCCTTTTCG‑‑GGTTAAACCCCCCCCCCCCTGGTTTGCCG‑CC‑ATTTTCAATTTTGGTGCTGTACCGTTAGGTGG‑GGTTTGCCCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCAttt                                                                       <  2:3223686/150‑1 (MQ=255)

                                                                                     tGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTGAA‑AACCCGCCAACACGTAGTACG‑CC‑CCATTCAAAGGTGGTGGCGTACCGGTTGGGGC‑GGGGGTTCCCCCGCAAGGGCCCGCGCCGGGTGTTGAGGTGGAACCttttttgg                                                                  >  2:843466/1‑148 (MQ=255)

                                                                                          gTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑ACCGCGCCTAAACGGCTTAAG‑CC‑CAGTTAAAATGTGGTGCCGGACCGGTAGGAGC‑GGGTTTAACCGCGCAAGGGCCGGCGCGGGGTGGAGAGATGCCTGATTTTTTCGACggg                                                             >  2:3095040/1‑150 (MQ=255)

                                                                                               aTAGCGCCTGTGCCGGCCCATTCGC‑GGGTAAA‑ACCGCACCAAAAGGTGTTACC‑CC‑CCATTCAAAACTGGGGCCGTACCGGTGGGAGC‑GGGGTTACCCCCGCAAGGGCCGGCGCCGTTTGGAGCGGAGCCACATTTTTTAGAAGGGCtttt                                                        >  2:3128043/1‑149 (MQ=255)

                                                                                                       tgtgCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                     <  2:508792/81‑1 (MQ=255)

                                                                                                       tgtgCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                     >  1:508792/1‑81 (MQ=255)

                                                                                                            cGGCCCTTTCGG‑GGCAAAA‑CCCGCACCTCCTGTTATTACC‑CC‑CTTTTCACTTGTGGTGCCTAACCTTTCGGCGG‑GGTTTAACCCGCGAACGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCt                                           <  2:1990483/150‑1 (MQ=255)

                                                                                                                 cTTTCGC‑GGGTAAA‑ACCGCTCCTACAGGTATTAAG‑CC‑CCTTTCAAATGTGGTGCAGTACCTGTATGAGC‑GGGTTTAACCGCGAAAGGGCCGGCGCAGTTTTTAGAGATGCAACAATTTTTATAAGGGCTTTATTAATCTGATTCTATGTa                                      >  2:2073681/1‑150 (MQ=255)

                                                                                                                    tCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCCGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCg                                   >  2:266095/1‑150 (MQ=255)

                                                                                                                      gc‑gGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTAACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAc                                 >  2:364614/1‑150 (MQ=255)

                                                                                                                            tAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCgg                            >  1:4235469/1‑150 (MQ=255)

                                                                                                                               a‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTc                         >  1:176582/1‑150 (MQ=255)

                                                                                                                                ccccGCGCCGACAGGTATTCCG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGG‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTc                         <  2:2625821/149‑1 (MQ=255)

                                                                                                                                 cccGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg                        >  1:561863/1‑150 (MQ=18)

                                                                                                                                  ccGCTCCTACAGGTATTACG‑CC‑CATTTCACATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGGTTGTAGAGATGCATCATTTTTTCGACGGGCGTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                       >  2:3996063/1‑150 (MQ=255)

                                                                                                                                  ccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                       >  1:4409362/1‑150 (MQ=255)

                                                                                                                                  ccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                       >  1:3061460/1‑150 (MQ=255)

                                                                                                                                  ccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                       >  1:3331845/1‑150 (MQ=255)

                                                                                                                                     cTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCa                    >  1:3821846/1‑150 (MQ=18)

                                                                                                                                     cTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCa                    >  1:3821816/1‑150 (MQ=18)

                                                                                                                                      tCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGa                                                                                                                          <  1:322888/48‑1 (MQ=255)

                                                                                                                                      tCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTGTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGGATCGACCCCGGCTCGCGCAt                   >  1:2026848/1‑150 (MQ=18)

                                                                                                                                        cTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTCGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCa                 >  1:1897715/1‑150 (MQ=18)

                                                                                                                                         tACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                >  2:393620/1‑150 (MQ=255)

                                                                                                                                            aGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCgg             >  1:2995928/1‑150 (MQ=255)

                                                                                                                                             ggTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGc            >  1:1038516/1‑150 (MQ=255)

                                                                                                                                              gTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCt           >  1:3694618/1‑150 (MQ=255)

                                                                                                                                              gTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCt           >  1:2511448/1‑150 (MQ=255)

                                                                                                                                                   aCG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGc      >  1:241693/1‑150 (MQ=255)

                                                                                                                                                     g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt    >  1:479090/1‑150 (MQ=255)

                                                                                                                                                     g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt    >  2:3719808/1‑150 (MQ=255)

                                                                                                                                                     g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt    >  1:2877975/1‑150 (MQ=255)

                                                                                                                                                     g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt    >  1:1821022/1‑150 (MQ=255)

                                                                                                                                                     g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt    >  2:2821119/1‑150 (MQ=255)

                                                                                                                                                        c‑cATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTgg  >  2:1577085/1‑150 (MQ=255)

                                                                                                                                                        c‑cATTTCAAAGGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTgg  >  2:144029/1‑150 (MQ=255)

TGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGG  >  NC_002947/1392778‑1393076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`