BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,291,559	A→T	7.5%	L246H (CTC→CAC) ‡	PP_4663 ←	transmembrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,291,559	0	A	T	7.5%	617.2 / 19.9	225	L246H (CTC→CAC) ‡	PP_4663	transmembrane protein
Reads supporting (aligned to +/- strand): ref base A (113/76); new base T (7/11); total (120/105)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

AAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATCCTGC  >  NC_002947/5291410‑5291705

aaGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACCTGGCCGCCTTCTTCGAAAAGCGCATGGACGACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGa                                                                                                                                                    <  2:3996751/150‑1 (MQ=255)

 aGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAg                                                                                                                                                   <  1:2427300/150‑1 (MQ=255)

  ggCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAgg                                                                                                                                                  <  1:871788/150‑1 (MQ=255)

     cgcgCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGggcg                                                                                                                                               >  2:642241/1‑150 (MQ=255)

        gcTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGcc                                                                                                                                            <  1:566054/150‑1 (MQ=255)

        gcTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGcc                                                                                                                                            <  1:3533517/150‑1 (MQ=255)

          tGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCCTCGGCGGCCac                                                                                                                                          >  2:4129708/1‑149 (MQ=255)

            gACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGc                                                                                                                                        <  2:872329/150‑1 (MQ=255)

             aCGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGcc                                                                                                                                       <  2:3053817/150‑1 (MQ=255)

               gTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGa                                                                                                                                     >  2:4206456/1‑150 (MQ=255)

                    cTCGGCCCTTCCGCACTTGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCTGGTGGCCGCCGAGGGCGGCCAGGCCGATGaaa                                                                                                                                <  2:3485835/150‑1 (MQ=255)

                     tCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGaaaa                                                                                                                               >  2:3315497/1‑150 (MQ=255)

                      cGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGTGCGCGATGTCGATATCCCGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTTAACAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGaaaaa                                                                                                                              <  2:2851115/150‑1 (MQ=255)

                      cGGCCCTTCCGCACAGGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCAAGGCCGATGaaaaa                                                                                                                              <  1:1323036/150‑1 (MQ=255)

                       ggCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAg                                                                                                                             >  2:744485/1‑150 (MQ=255)

                          ccgtCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCaa                                                                                                                          <  2:4444231/147‑1 (MQ=255)

                          ccTTCGGCACATGGCCGCCTTCTGCGAAAAGCGCATGGACTCCCTGGTGCGCTATTTCTATAGCCAGGGGGTCGACATGCTCAGCGCTACGTCCGCTAACCGTTGACCAGCAGGGGGCCGCCGTGGGCGGCCAGGCCGATGAAAAAGCaa                                                                                                                          <  2:1700580/150‑1 (MQ=255)

                           cTTCCGCACTTGGCCGCCTTCTCCCAAAAGCGCATGGACTACGTGGCGCGCGTTTTCGTTAACCCGGGGGTCGACCTGCTCAGCGCTTCGCCCGCTACCCGTTGATCAGCCGGTGGCCGCCGCGGGCGGCCAGGCCGATGAAAAAGCAAc                                                                                                                         <  1:3247982/150‑1 (MQ=255)

                            ttCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACg                                                                                                                        >  2:2626017/1‑150 (MQ=255)

                                  acaTGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGaa                                                                                                                  <  2:3004960/150‑1 (MQ=255)

                                      ggCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAAcagc                                                                                                              >  2:2871317/1‑150 (MQ=255)

                                       gccgccTTCTTCTAAAAGCGCATGGACTACCTGGAGCGCTATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAAcagca                                                                                                             <  2:4184357/150‑1 (MQ=255)

                                       gccgccTTCTTCGAAAAGCGCTTGGACTACCTGGAGCGCGATTTCGATATCCAGGGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAAcagca                                                                                                             <  1:4082829/150‑1 (MQ=255)

                                       gccgccTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGTTTTCGATATCCCGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAAcagca                                                                                                             <  2:3436021/150‑1 (MQ=255)

                                        ccgccTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAAcagcag                                                                                                            >  1:1759640/1‑150 (MQ=255)

                                        ccgccTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAAcagcag                                                                                                            >  1:3844595/1‑150 (MQ=255)

                                         cgccTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCTTTTTCGATATCCAGCGGGTCGACTTGCTCAGCGCTTCGTCCGATAACCGTTGATCAGCAGGTGGCCGCCGCGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACagcagc                                                                                                           <  1:4017574/150‑1 (MQ=255)

                                         cgccTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACagcagc                                                                                                           >  2:1573534/1‑150 (MQ=255)

                                          gccTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAgcg                                                                                                          >  2:4295385/1‑150 (MQ=255)

                                            cttcttCGAAACGCGCATGGACTACCTGGAGCGCGATTTCGATTTCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAgcgcg                                                                                                        <  2:619579/150‑1 (MQ=255)

                                                ttcGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGa                                                                                                    >  1:1887051/1‑150 (MQ=255)

                                                 tcGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTGAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGAt                                                                                                   >  1:3746358/1‑150 (MQ=255)

                                                 tcGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGAt                                                                                                   >  2:2805901/1‑150 (MQ=255)

                                                      aaGCGACTGGACTCCGTGGTGCGCTTTTTCGATATCCCGGGGGTCGACATGCTCCGCGCTTGGGCCGCTACCCGTGGATCAGCTGGGGGCCGCCGCGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCt                                                                                              <  1:2674420/150‑1 (MQ=255)

                                                           cATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCCGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGc                                                                                         >  1:2861902/1‑150 (MQ=255)

                                                           cATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGc                                                                                         >  2:1049005/1‑150 (MQ=255)

                                                              ggACTACCTGGTGCGCTATTTCGATATCCCGCGGGTCTACATGCTCAGCGCTTCGTCCGCTAACCGTTGAACCGCCGGTGGCCGCCGCGGGGGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGc                                                                                      <  1:3087017/150‑1 (MQ=255)

                                                                aCTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCa                                                                                    <  2:3678587/150‑1 (MQ=255)

                                                                  tACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACt                                                                                  >  2:3068356/1‑150 (MQ=255)

                                                                   ccgtGGCGCGCGATTTCGATATCCGGGGGGGCGACCGGCTCAGGGCTTCGTCCGCTAACGGTGGATCCGCGGGGGGCCGCGGGGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTg                                                                                 <  1:3991977/147‑1 (MQ=255)

                                                                   aCCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTg                                                                                 <  1:1839858/150‑1 (MQ=255)

                                                                    ccTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTgg                                                                                >  2:3187698/1‑150 (MQ=255)

                                                                      tGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCAACTCGcc                                                                              >  1:3452104/1‑150 (MQ=255)

                                                                           cgcgATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCa                                                                         >  1:3607138/1‑150 (MQ=255)

                                                                             cgATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGACAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGc                                                                       >  2:3076218/1‑150 (MQ=255)

                                                                             cgATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGc                                                                       >  2:806867/1‑150 (MQ=255)

                                                                                tttCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATg                                                                    >  1:2750056/1‑150 (MQ=255)

                                                                                   cGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCa                                                                                                                                           <  1:1583187/76‑1 (MQ=255)

                                                                                   cGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCg                                                                 >  1:231558/1‑150 (MQ=255)

                                                                                   cGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCg                                                                 >  1:2516781/1‑150 (MQ=255)

                                                                                    tatatCCCGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCCGCTGGTGGCCGCCGCGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGa                                                                <  1:3839116/149‑1 (MQ=255)

                                                                                    gATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGa                                                                >  2:3738967/1‑150 (MQ=255)

                                                                                    gATATCCAGCGGGTCGACATGCTCAGCGCTCCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGa                                                                <  2:1290978/150‑1 (MQ=255)

                                                                                      tatCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGc                                                              <  1:4110636/150‑1 (MQ=255)

                                                                                        tCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTTATCAGCAGGTGGCCGCCGTGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCaa                                                            <  1:668417/150‑1 (MQ=255)

                                                                                         ccAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAg                                                           <  2:3469585/150‑1 (MQ=255)

                                                                                                gTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGgcg                                                    >  2:1820578/1‑150 (MQ=255)

                                                                                                gTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGgcg                                                    >  2:3189307/1‑150 (MQ=255)

                                                                                                   gACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCa                                                 <  2:1581775/150‑1 (MQ=255)

                                                                                                   gACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCa                                                 <  2:2606279/150‑1 (MQ=255)

                                                                                                    aCATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAg                                                >  1:1634774/1‑150 (MQ=255)

                                                                                                    aCATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAg                                                >  1:1634586/1‑150 (MQ=255)

                                                                                                    aCATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAg                                                <  2:241517/150‑1 (MQ=255)

                                                                                                    aCATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAg                                                >  1:142459/1‑150 (MQ=255)

                                                                                                       tGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCAt                                             >  2:2583995/1‑150 (MQ=255)

                                                                                                         cTCAGCGCTTCGTCCGCTAACCGTTGATCAGCTGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATca                                           <  2:530628/150‑1 (MQ=255)

                                                                                                          tCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCCGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATcag                                          >  1:3173533/1‑150 (MQ=255)

                                                                                                          tCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATcag                                          >  1:164684/1‑150 (MQ=255)

                                                                                                          tCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATcag                                          >  1:164520/1‑150 (MQ=255)

                                                                                                            aGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATcagca                                        >  2:2372267/1‑150 (MQ=255)

                                                                                                             gcgcTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGCGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATcagcag                                       <  2:1118624/150‑1 (MQ=255)

                                                                                                              cgcTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCagcagc                                      >  2:1268758/1‑150 (MQ=255)

                                                                                                              cgcTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCagcagc                                      <  1:3315497/150‑1 (MQ=255)

                                                                                                              cgcTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCagcagc                                      >  1:1923962/1‑150 (MQ=255)

                                                                                                              cgcTTCGTCCGCTAACCGGTGATCAGCAGGTGGCCGCCGCGGGCGGCCAGGCCGATGAACAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCagcagc                                      <  2:2218658/150‑1 (MQ=255)

                                                                                                                   cGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGc                                 >  2:1817816/1‑150 (MQ=255)

                                                                                                                    gTCCGCTACCCGTGGATCCGCCGGGGGCCGCCGGGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGcc                                <  1:2002157/150‑1 (MQ=255)

                                                                                                                     tCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGccc                               >  1:1018337/1‑150 (MQ=255)

                                                                                                                      ccGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGcccc                              <  1:1054318/150‑1 (MQ=255)

                                                                                                                      ccGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGcccc                              <  2:1535752/150‑1 (MQ=255)

                                                                                                                       cGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGccccc                             >  2:1188112/1‑150 (MQ=255)

                                                                                                                         cTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAg                           >  1:4202460/1‑150 (MQ=255)

                                                                                                                          tAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAgc                          >  2:559009/1‑150 (MQ=255)

                                                                                                                          tAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAgc                          <  1:4206456/150‑1 (MQ=255)

                                                                                                                           aaCCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAgcg                         <  1:4510358/150‑1 (MQ=255)

                                                                                                                                  gATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGcc                  <  2:48099/150‑1 (MQ=255)

                                                                                                                                    tCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCAc                >  2:4329106/1‑150 (MQ=255)

                                                                                                                                    tCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCAc                >  1:2143559/1‑150 (MQ=255)

                                                                                                                                    tCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCAc                >  2:2072804/1‑150 (MQ=255)

                                                                                                                                     cagcagGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCAcc               <  2:623190/150‑1 (MQ=255)

                                                                                                                                       gcagGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCa             >  2:3128328/1‑150 (MQ=255)

                                                                                                                                       gcagGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAAAGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCa             >  1:2696444/1‑150 (MQ=255)

                                                                                                                                        gagGTGGCCGCCGAGGGGGGCCAGGCCGATGAAAAAGCAACTCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGTCCGTCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCaa            <  2:1925078/149‑1 (MQ=255)

                                                                                                                                        ccggTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCaa            <  1:3096900/148‑1 (MQ=255)

                                                                                                                                         gcctGGCCGCCCTCGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAg           >  2:3580245/4‑150 (MQ=255)

                                                                                                                                          ggTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAgg          <  2:1480823/150‑1 (MQ=255)

                                                                                                                                            tGGCCGCCCTCGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCa        >  1:3728402/1‑150 (MQ=255)

                                                                                                                                             ggCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCAt       >  2:3788142/1‑150 (MQ=255)

                                                                                                                                              gccgccGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATc      <  1:3437214/150‑1 (MQ=255)

                                                                                                                                                  ccgAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATCCTGc  <  2:2556246/150‑1 (MQ=255)

AAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAGGTGGCCGCCGAGGGCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATCCTGC  >  NC_002947/5291410‑5291705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`