Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,392,401 T→G 5.6% intergenic (+520/‑202) PP_4738 → / → PP_mr56 hypothetical protein/C4 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,392,4010TG 5.6% 624.6 / 16.6 206intergenic (+520/‑202)PP_4738/PP_mr56hypothetical protein/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base T (113/80);  new base G (9/4);  total (122/84)
Fisher's exact test for biased strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

CAACTCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCG  >  NC_002947/5392257‑5392549
                                                                                                                                                |                                                                                                                                                    
caACTCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGc                                                                                                                                                 <  2:3668142/150‑1 (MQ=255)
 aaCTCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCg                                                                                                                                                >  1:947747/1‑150 (MQ=255)
 aaCTCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCGCGGGCg                                                                                                                                                >  1:134960/1‑150 (MQ=255)
    tCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTcg                                                                                                                                             >  1:189302/1‑149 (MQ=255)
    tCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCt                                                                                                                                             >  2:1927872/1‑150 (MQ=255)
     cATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCtt                                                                                                                                            <  1:422818/150‑1 (MQ=255)
      aTCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCGAGGGCGTCttc                                                                                                                                           <  1:1807964/150‑2 (MQ=255)
         cAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCtttttt                                                                                                                                        >  2:576078/1‑150 (MQ=255)
          aaaGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCttttttt                                                                                                                                       >  1:1019499/1‑150 (MQ=255)
          aaaGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCttttttt                                                                                                                                       >  1:1265329/1‑150 (MQ=255)
          aaaGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCttttttt                                                                                                                                       >  2:3967090/1‑150 (MQ=255)
          aaaGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCttttttt                                                                                                                                       >  2:813531/1‑150 (MQ=255)
          aaaGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCttttttt                                                                                                                                       <  2:1617853/150‑1 (MQ=255)
           aaGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTtg                                                                                                                                      <  2:3929015/150‑1 (MQ=255)
            aGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTtgt                                                                                                                                     <  1:3423153/150‑1 (MQ=255)
            aGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAAACCAAAGAAAAAGACGCCCGAGGGCGTcttcttcttt                                                                                                                                     <  1:1696086/150‑5 (MQ=255)
                     cccGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCGCGGGCGTCTTTTGTTGTGGCTGGGAt                                                                                                                            >  1:1097038/1‑150 (MQ=255)
                        gTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTg                                                                                                                         <  2:33835/150‑1 (MQ=255)
                           gggCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGgc                                                                                                                      >  2:2889297/1‑150 (MQ=255)
                           gggCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGgc                                                                                                                      >  2:3738253/1‑150 (MQ=255)
                             gcggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGgcgc                                                                                                                    >  2:2554518/1‑150 (MQ=255)
                               ggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTg                                                                                                                  >  2:842011/1‑150 (MQ=255)
                               ggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTg                                                                                                                  >  2:2221735/1‑150 (MQ=255)
                                 cTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTAGCGAGAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATGCAAAGAAGAAGACGGCGTCGGGGGTGTTTTTTTGTGGGTGGTATTTGGGGGCTGCg                                                                                                                >  2:272075/1‑150 (MQ=255)
                                     ggATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTc                                                                                                            <  2:564834/150‑1 (MQ=255)
                                          aaaTCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGaaaa                                                                                                       >  2:3596609/1‑150 (MQ=255)
                                           aaTCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGaaaaa                                                                                                      >  2:582264/1‑150 (MQ=255)
                                                gTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGa                                                                                                 <  1:244356/150‑1 (MQ=255)
                                                gTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGa                                                                                                 <  2:1798591/150‑1 (MQ=255)
                                                           tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCtt                                                                                      >  2:2238367/1‑150 (MQ=255)
                                                            aaGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCttt                                                                                     <  1:3023936/150‑1 (MQ=255)
                                                                 tGTAGTACCGACAGCGGAGTACTGTCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTTTGGCTGGAATTTTGGCGCTGCGGGTCGTAAAATCGGTTTTAACCCCTTtgttgt                                                                                >  1:2741811/1‑150 (MQ=255)
                                                                 tGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGt                                                                                <  2:1013163/150‑1 (MQ=255)
                                                                  gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTa                                                                               <  2:765787/150‑1 (MQ=255)
                                                                  gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTa                                                                               <  1:2224883/150‑1 (MQ=255)
                                                                       aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCg                                                                          <  2:1597960/150‑1 (MQ=255)
                                                                              gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCa                                                                   >  2:1327806/1‑150 (MQ=255)
                                                                              gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTGTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCa                                                                   >  2:1838807/1‑150 (MQ=255)
                                                                               cGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCaa                                                                  <  1:401185/150‑1 (MQ=255)
                                                                                 gAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAg                                                                >  2:3132784/1‑150 (MQ=255)
                                                                                    tACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATa                                                             >  2:401197/1‑150 (MQ=255)
                                                                                     aCTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATAc                                                            <  2:2447242/150‑1 (MQ=255)
                                                                                     aCTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATAc                                                            >  2:51288/1‑150 (MQ=255)
                                                                                        ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgc                                                         >  1:1765621/1‑150 (MQ=255)
                                                                                         gCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgcc                                                        >  2:68280/1‑150 (MQ=255)
                                                                                           ggACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTTCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTGTTTTAGGGCGTTTCCCAAAGATAatgccag                                                      >  1:3844306/1‑148 (MQ=255)
                                                                                                   gTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGtt                                              >  2:2641950/1‑150 (MQ=255)
                                                                                                   gTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGtt                                              >  2:2342894/1‑150 (MQ=255)
                                                                                                   gTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGtt                                              <  2:2153868/150‑1 (MQ=255)
                                                                                                   gTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGCAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGtt                                              >  2:3090521/1‑150 (MQ=255)
                                                                                                      cAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTc                                           <  1:1363869/150‑1 (MQ=255)
                                                                                                      cAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTc                                           <  2:4198186/150‑1 (MQ=255)
                                                                                                      cAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTc                                           <  2:803100/150‑1 (MQ=255)
                                                                                                       aaaTCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTcc                                          <  1:183393/150‑1 (MQ=255)
                                                                                                       aaaTCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTcc                                          >  1:4455709/1‑150 (MQ=255)
                                                                                                         aTCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGt                                        >  2:4253743/1‑150 (MQ=255)
                                                                                                          tCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGtg                                       >  2:3880730/1‑149 (MQ=255)
                                                                                                          gccccccGGCTCCACCAACTCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGtt                                       <  2:2523919/149‑1 (MQ=255)
                                                                                                           ccccccGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTg                                      <  2:4335950/150‑1 (MQ=255)
                                                                                                             ccccGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCg                                    >  1:4460425/1‑150 (MQ=255)
                                                                                                              cccGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                                   >  2:830760/1‑150 (MQ=255)
                                                                                                                cGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTACGTTGCGGTc                                 >  1:1198162/1‑150 (MQ=255)
                                                                                                                    tCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTTCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttt                             >  1:2365821/1‑150 (MQ=255)
                                                                                                                       accaAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAtttttt                          >  1:2810388/1‑150 (MQ=255)
                                                                                                                        ccaAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTc                         >  2:1364484/1‑150 (MQ=255)
                                                                                                                        ccaAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTc                         <  1:3361399/150‑1 (MQ=255)
                                                                                                                         cacaTCCAAAGACGAAGACGCCCTCGGGCGTCTTATTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAACTCGGATTTAACCCCTTACTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCa                        <  2:1097038/147‑1 (MQ=255)
                                                                                                                         caAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCa                        >  2:2500065/1‑150 (MQ=255)
                                                                                                                         caAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCa                        >  1:1850794/1‑150 (MQ=255)
                                                                                                                         caAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCa                        >  1:649226/1‑150 (MQ=255)
                                                                                                                          aaaTCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAg                       <  1:576084/150‑1 (MQ=255)
                                                                                                                           aaTCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAgg                      <  2:2865346/150‑1 (MQ=255)
                                                                                                                           aaTCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAgg                      >  2:4174557/1‑150 (MQ=255)
                                                                                                                              ccAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGtt                   >  2:1960245/1‑150 (MQ=255)
                                                                                                                               cAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGttt                  <  1:813531/150‑1 (MQ=255)
                                                                                                                               cAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCATTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTACGTTGCGGTCATTTTTTAAGGGttt                  >  1:260659/1‑150 (MQ=255)
                                                                                                                                aaaGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGtttt                 >  2:2802724/1‑150 (MQ=255)
                                                                                                                                 aagaagaagACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTa                >  2:1655406/1‑150 (MQ=255)
                                                                                                                                   gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGt              <  2:947747/150‑1 (MQ=255)
                                                                                                                                   aaaaaagaCGCCCGAGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGt              >  2:1528486/5‑150 (MQ=255)
                                                                                                                                    aagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtc             >  2:3337194/1‑150 (MQ=255)
                                                                                                                                    aagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAACCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTACGTTGCGGTCATTTTTTCAGGGTTTTAGtg             >  1:2700579/1‑149 (MQ=255)
                                                                                                                                    aaaaagaCGCCCGAGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGAtt                                                                                               >  2:3625898/4‑68 (MQ=38)
                                                                                                                                    aaaaagaCGCCCGAGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGAtt                                                                                               <  1:3625898/65‑1 (MQ=38)
                                                                                                                                      gaagaCGCCCTCGGGCGTCTTTTTTTGTGCCGGGAATTTGGGCGCGGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGGTTTAGtctc           >  2:3571316/1‑150 (MQ=255)
                                                                                                                                      aaagaCGCCCGAGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc           >  1:2434379/2‑150 (MQ=255)
                                                                                                                                        agaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCGGTTTTCCGTTGCGGTCATTTTTTCCGGGGTTTAGGCTCgg         >  1:964396/1‑150 (MQ=255)
                                                                                                                                         gaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCGGGGTTTTAGTCTCGGt        <  2:216665/150‑1 (MQ=255)
                                                                                                                                         gaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGt        >  2:1992826/1‑150 (MQ=255)
                                                                                                                                         gaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCAGCGCCGGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGt        >  1:3122667/1‑150 (MQ=255)
                                                                                                                                         gaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCATTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGt        >  1:1952271/1‑150 (MQ=255)
                                                                                                                                          aCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTg       <  2:4074788/150‑1 (MQ=255)
                                                                                                                                           cGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTgg      <  1:1061036/150‑1 (MQ=255)
                                                                                                                                           cGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTgg      >  1:1483969/1‑150 (MQ=255)
                                                                                                                                           cGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTgg      <  1:4397579/150‑1 (MQ=255)
                                                                                                                                             cccTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGt    <  1:1642088/150‑1 (MQ=255)
                                                                                                                                              ccTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGTCGCTGCGGGTCGAAAAATAGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTAAGGGTTTTAGTCTCGGTGGGTc   >  1:1115560/1‑150 (MQ=255)
                                                                                                                                              ccTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTc   <  2:2072035/150‑1 (MQ=255)
                                                                                                                                               cTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCg  >  2:646242/1‑150 (MQ=255)
                                                                                                                                               cTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCg  >  1:3705893/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CAACTCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCG  >  NC_002947/5392257‑5392549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑