BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,527,449	T→C	7.1%	R89R (CGT→CGC)	PP_4858 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,527,449	0	T	C	7.1%	388.3 / 13.7	145	R89R (CGT→CGC)	PP_4858	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (62/67); new base C (3/7); total (70/75)
Fisher's exact test for biased strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

Only 100 of 148 total aligned reads displayed.

CAGCCGCTGAACACCGTAGCTGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCGTGTTGCCGAAGGCGG  >  NC_002947/5527300‑5527598

 aGCCGCTGAACACCGTAGCTGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCgtg                                                                                                                                                      >  1:4495908/1‑150 (MQ=255)

            acCGTAGCTGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGt                                                                                                                                           <  1:1314396/150‑1 (MQ=255)

            acCGTAGCTGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGt                                                                                                                                           <  1:1616758/150‑1 (MQ=255)

            acCGTAGCTGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGt                                                                                                                                           <  1:3702470/150‑1 (MQ=255)

              cGTAGCTGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTgg                                                                                                                                         <  2:1372627/150‑1 (MQ=255)

              cGTAGCTGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTgg                                                                                                                                         <  1:2434561/150‑1 (MQ=255)

              cGTAGCTGAAGGCGGCTCCGATCGCCTGATCGAACGGTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTgg                                                                                                                                         <  2:1212876/150‑1 (MQ=255)

              cGTAGCGGCAGGCGGCTCCGATGGCCTGTTCGAACGTCCGGGCCGCGTTGCTGTAGGGGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTgg                                                                                                                                         <  2:2549636/150‑1 (MQ=14)

               gTAGCTGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGc                                                                                                                                        >  2:220125/1‑150 (MQ=17)

                 aGCTGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTc                                                                                                                                      <  1:2697735/150‑1 (MQ=40)

                        ggcggcTCCGATCGCCTGATCGAACGTTCGGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGt                                                                                                                               <  2:1116997/150‑1 (MQ=255)

                              tCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTGCTGGACGGGTAGCTGAAGGTGGCTCGGATCGCCTGTTCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATc                                                                                                                         >  2:180110/1‑150 (MQ=11)

                                cGATCGCCTGTTCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGa                                                                                                                       <  2:4343483/150‑1 (MQ=37)

                                 gATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGAACGCCTGATCGAACGTTCTGGCCGCGTTGCAGAAGGTGGCTCCGATAGACTGATCGAACATACTGGCCGGGTTGCGGAAGGAGGCTCCGATAGTCTGATAGaa                                                                                                                      >  2:596739/1‑150 (MQ=11)

                                   tCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACg                                                                                                                    >  2:4125927/1‑150 (MQ=37)

                                     gCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGtt                                                                                                                  <  2:3233982/150‑1 (MQ=37)

                                     gCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGtt                                                                                                                  <  2:772807/150‑1 (MQ=37)

                                      ccTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGGCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGtgc                                                                                                                 >  2:2368503/1‑148 (MQ=21)

                                       cTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCt                                                                                                                >  1:299046/1‑150 (MQ=37)

                                        tgctCGACCGGTCGGGCCGCGTTGCTGAAGGGGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTg                                                                                                               <  2:4426307/147‑1 (MQ=11)

                                         gATCGACCGGTCGGGCCGCGTTGCGGAAGGGGGCTCCGCTCGCCTGATCGACCGGTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTgg                                                                                                              <  2:4043382/150‑1 (MQ=11)

                                         gATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTgg                                                                                                              >  1:3611723/1‑150 (MQ=37)

                                         gATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTgg                                                                                                              <  2:3800799/150‑1 (MQ=37)

                                             gAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCAGATCGAACGTTCTGGCcgc                                                                                                          >  1:4155927/1‑150 (MQ=38)

                                                 gTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTg                                                                                                      >  2:1238891/1‑150 (MQ=21)

                                                 gTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTg                                                                                                      >  2:323902/1‑150 (MQ=21)

                                                  ttCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGc                                                                                                     <  1:2284301/150‑1 (MQ=39)

                                                  gtCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGc                                                                                                     <  2:4477537/149‑1 (MQ=39)

                                                   tCTTGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCt                                                                                                    <  2:2988480/150‑1 (MQ=255)

                                                      ggCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGaa                                                                                                 <  1:3023970/150‑1 (MQ=255)

                                                       gCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGATCTGGCCGCGTAGCTGAAGGTGGCTCCGATCGCCTGATCGAACGGTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTCGCTGAAg                                                                                                >  1:1221460/1‑150 (MQ=255)

                                                         cgcgTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTc                                                                                                                                      >  1:1251487/1‑110 (MQ=40)

                                                         cgcgTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGt                                                                                              <  1:1120357/150‑1 (MQ=255)

                                                         cgcgTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGt                                                                                              <  1:2753813/150‑1 (MQ=255)

                                                                 tGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTACTGGCCGCGTTGCTGAAGGTGGCTCCGa                                                                                      >  1:2665009/1‑150 (MQ=255)

                                                                 tGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGa                                                                                      <  1:1098895/150‑1 (MQ=21)

                                                                   aaGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATc                                                                                    <  2:3445536/150‑1 (MQ=39)

                                                                   aaGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATc                                                                                    <  1:4000366/150‑1 (MQ=39)

                                                                      ggggCTCCGATCGCCTGATCGAACGTTCTGTCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTc                                                                                 <  2:2459776/148‑1 (MQ=37)

                                                                      gTGGCTCCGATCGCCTGATCGACCGTACTGGCCGCGTTGCTGAAGGGGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTc                                                                                 <  2:214944/150‑1 (MQ=255)

                                                                        tgCTCTGATCTCCTGTTCGAACGTTCTTCCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTg                                                                               <  2:410992/149‑1 (MQ=11)

                                                                        ggCTCCGATCCCATGTCCGATCGTCCTTGCAGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTg                                                                               <  2:1576684/150‑1 (MQ=11)

                                                                         gCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGa                                                                              <  2:4238916/150‑1 (MQ=37)

                                                                         gCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGATCTGGCCGCGTTGCGGAAGGAGGCTCCGATCGTCTGa                                                                              >  2:3997323/1‑150 (MQ=255)

                                                                          cTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGAt                                                                             <  2:2789411/150‑1 (MQ=37)

                                                                           tCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATc                                                                            >  1:4214749/1‑150 (MQ=37)

                                                                           tCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGGTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCTAACGGTCTGGCCGGGTTGCTGAAGGTGGCTCCGATCGACTGAtg                                                                            >  2:3150490/1‑149 (MQ=11)

                                                                           tCCGATCGCCTGATCGAACGTTCTGGCCGCGTAGCTGAAGGTGGCTCCGATCGCCTGATCGAACTATCTGGCCGAGTTGCGGAAGGTGGCTCCGATCGACTGATCGAACTGTCTGGCCGCGTAGCTGAAGGTGGCTCCGATCGTCTGGTc                                                                            >  1:1314987/1‑150 (MQ=11)

                                                                             cGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGa                                                                          <  2:4051067/150‑1 (MQ=37)

                                                                                       aTCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGATCTGGc                                                                >  1:1917730/1‑150 (MQ=37)

                                                                                          gAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGGGTTGCGGAAGGTGGCTCCGATCGTCTGATCGAACAATCTGGCCgt                                                             >  2:2722908/1‑150 (MQ=255)

                                                                                             cGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGtt                                                          <  2:1570362/150‑1 (MQ=39)

                                                                                               ttCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGc                                                        <  2:3955568/150‑1 (MQ=39)

                                                                                                    gCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACTATCTGGCCGTGTTGCTGAAg                                                   >  2:397735/1‑150 (MQ=255)

                                                                                                    gCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAg                                                   <  2:3049931/150‑1 (MQ=255)

                                                                                                    gCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGATCTGGCCGTGTTGCAGAAg                                                   >  2:1191917/1‑150 (MQ=255)

                                                                                                    ccagcgTTGCTGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGAGTTGCTGAAGGTGGCTCCGATTGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAg                                                   <  2:3049767/147‑1 (MQ=255)

                                                                                                     ccGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAgg                                                  >  1:2642542/1‑150 (MQ=255)

                                                                                                       gagTTGCTGTAGGTTGCTCAGATCGACTGATCGATCGTTCTTTCCGTGTTGCTGAAGGTGTCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTg                                                <  2:3900926/148‑1 (MQ=14)

                                                                                                           tgctgcAGCGGGCTCCGATCGCCTGATCGAACGGTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTc                                            <  2:3210077/150‑1 (MQ=11)

                                                                                                           tGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTc                                            <  2:1778372/150‑1 (MQ=37)

                                                                                                            gCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCg                                           >  2:2297129/1‑150 (MQ=255)

                                                                                                              tGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGa                                         >  2:361739/1‑150 (MQ=255)

                                                                                                              tGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGa                                         >  1:3783953/1‑150 (MQ=255)

                                                                                                              tGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGa                                         >  1:1543970/1‑150 (MQ=255)

                                                                                                              tGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGGGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTCGCTGAAGGTg                                                >  2:718796/1‑143 (MQ=255)

                                                                                                               gAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGAt                                        >  2:243402/1‑150 (MQ=255)

                                                                                                               gAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGATCTGGCCGTGTTGCCGAAGGAGGCTCGGAt                                        >  2:2995193/1‑150 (MQ=255)

                                                                                                                caGGTGGCTCCGATCGCCTGATCGAACGGTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATc                                       <  2:2524787/149‑1 (MQ=255)

                                                                                                                aaGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGGGTTGCTGAAGGTGGCTCCGATCGTCGGATCGAAGGTTCTGGCCGCGGTGCGGACGGTGGCTCCGATCGTCTGATCGAACGGTCTGGCCGGGTTGCCGAGGGTGGCTCGGATc                                       >  2:3852793/1‑150 (MQ=14)

                                                                                                                     ggCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTg                                  >  2:2728670/1‑150 (MQ=255)

                                                                                                                      gCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGa                                 >  2:619255/1‑150 (MQ=255)

                                                                                                                       cTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTGTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGAt                                >  1:954147/1‑150 (MQ=255)

                                                                                                                        tCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATc                               >  1:896776/1‑150 (MQ=255)

                                                                                                                        tCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATc                               >  1:472598/1‑150 (MQ=255)

                                                                                                                        tCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATc                               <  2:3611951/150‑1 (MQ=37)

                                                                                                                            aTCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAAc                           >  1:3206406/1‑150 (MQ=255)

                                                                                                                               gCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAg                        <  2:2463685/150‑1 (MQ=255)

                                                                                                                                ccTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGc                       >  2:385538/1‑150 (MQ=255)

                                                                                                                                ccTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGc                       >  2:2950731/1‑150 (MQ=255)

                                                                                                                                  tGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACTTACTGGCCGCGTTGCAGAACGTGGCTCCGATAGTCTGAGAGAACTGACTCGCCGTGGTGCCGAACGTGGCGCGGATCGTCAGATCGAAGAACCTg                     >  2:3148928/1‑150 (MQ=14)

                                                                                                                                    aTCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGc                   >  1:3322101/1‑150 (MQ=255)

                                                                                                                                    aTCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGCGGCTCGGATCGTCTGATCGAACAAGCTGGc                   >  2:674934/1‑150 (MQ=255)

                                                                                                                                     tCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGcc                  <  2:2305217/150‑1 (MQ=255)

                                                                                                                                     tCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGcc                  <  2:22972/150‑1 (MQ=255)

                                                                                                                                      cGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGGGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACACGCTGGCCg                 >  1:3563742/1‑150 (MQ=255)

                                                                                                                                       gAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCgt                >  1:555210/1‑150 (MQ=255)

                                                                                                                                       gAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCgt                >  1:3857577/1‑150 (MQ=255)

                                                                                                                                       gAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCgt                >  1:1807075/1‑150 (MQ=255)

                                                                                                                                       gAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGATCTGGCCGAGTAGCTGAAGGTGGCTCCGATCGACTGGTAGAACGATCACGACGTGTAGCCGAACCAGGCGCGGAACGTCACAACGAACACGCTAGCCgt                >  1:217998/1‑150 (MQ=9)

                                                                                                                                        aaCGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGATCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCgtg               >  1:1122909/1‑150 (MQ=255)

                                                                                                                                            ttCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTTGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCGTGTTGc           <  2:3928555/150‑1 (MQ=255)

                                                                                                                                             tCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCGTGTTGcc          <  2:3485831/150‑1 (MQ=255)

                                                                                                                                              cTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCGTGTTGCCg         >  2:1281223/1‑150 (MQ=255)

                                                                                                                                                ggCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCGTGTTGCCGaa       >  2:3193111/1‑150 (MQ=255)

                                                                                                                                                 gCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCGTGTTGCCGAAg      >  2:4004244/1‑150 (MQ=255)

                                                                                                                                                 gCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCGTGTTGCCGAAg      <  2:596581/150‑1 (MQ=255)

                                                                                                                                                  ccGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCGTGTTGCCGaacg     >  1:139800/1‑148 (MQ=255)

                                                                                                                                                  ccGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCGTGTTGCCGAAgg     <  1:611993/150‑1 (MQ=255)

                                                                                                                                                  ccGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCGTGTTGCCGAAgg     <  1:600065/150‑1 (MQ=255)

CAGCCGCTGAACACCGTAGCTGAAGGCGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGCCTGATCGAACGTTCTGGCCGTGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGCGTTGCTGAAGGTGGCTCCGATCGTCTGATCGAACGTTCTGGCCGTGTTGCCGAAGGTGGCTCGGATCGTCTGATCGAACAAGCTGGCCGTGTTGCCGAAGGCGG  >  NC_002947/5527300‑5527598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`