BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,594,947	G→T	5.4%	Y360* (TAC→TAA) ‡	PP_4921 ←	NCS1 family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,594,947	0	G	T	5.4%	920.3 / 26.4	268	Y360* (TAC→TAA) ‡	PP_4921	NCS1 family transporter
Reads supporting (aligned to +/- strand): ref base G (107/146); new base T (5/10); total (112/156)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

TGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGCGG  >  NC_002947/5594800‑5595096

tGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTa                                                                                                                                                     >  2:3974184/1‑150 (MQ=255)

tGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTa                                                                                                                                                     <  2:3257202/150‑1 (MQ=255)

tGCCAGCAGCAATGCGGGCAGGGTGGCGCACAGTTCGGGCGGCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCCTCGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCTGACGGCGTCGACGGACCTCGTa                                                                                                                                                     <  2:1971203/150‑1 (MQ=255)

   cagcagCTATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTg                                                                                                                                                  <  2:1670199/150‑1 (MQ=255)

       agcaATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTcc                                                                                                                                              >  1:3636740/1‑150 (MQ=255)

        gcaATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCa                                                                                                                                             <  1:4349946/150‑1 (MQ=255)

        gcaATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCa                                                                                                                                             >  2:4259978/1‑150 (MQ=255)

         caATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACTACGTGGTccgt                                                                                                                                            <  1:3537887/150‑3 (MQ=255)

          aaTGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCGAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATc                                                                                                                                           >  1:1171788/1‑150 (MQ=255)

          aaTGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCAGGTAGTGGTCCATc                                                                                                                                           >  2:25512/1‑150 (MQ=255)

           aTGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCa                                                                                                                                          >  1:479209/1‑150 (MQ=255)

           aTGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCa                                                                                                                                          >  1:1790151/1‑150 (MQ=255)

             gCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGATGGACCACTACGTGGTCCGTcgac                                                                                                                                        <  1:2018844/150‑4 (MQ=255)

               gggCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGcac                                                                                                                                      >  1:3849768/1‑150 (MQ=255)

                       tGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGa                                                                                                                              >  1:1822033/1‑150 (MQ=255)

                       tGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGa                                                                                                                              <  1:3439548/150‑1 (MQ=255)

                        ggCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGac                                                                                                                             <  1:634637/150‑2 (MQ=255)

                         gcgcCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAAc                                                                                                                            >  2:4097090/1‑150 (MQ=255)

                           gcCCAGTTCGGGCGCCTTGGCGGCAAGCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCCACGCCTGCCAGTGCAAGCCCTTGACCTGTGCTGGCAGACGGCGGCGACGGACCACGGAGTGGTCCATCAGCACCACGCCgcccag                                                                                                                          >  2:4284466/1‑145 (MQ=255)

                            cccAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGc                                                                                                                         >  1:1432768/1‑150 (MQ=255)

                              cAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCgg                                                                                                                       >  2:4506891/1‑150 (MQ=255)

                              cAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGATGGCAGACGGCGACGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCgg                                                                                                                       >  2:3030445/1‑150 (MQ=255)

                               aGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGt                                                                                                                      <  1:3689811/150‑1 (MQ=255)

                                gTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTg                                                                                                                     <  2:2076675/150‑1 (MQ=255)

                                gTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTg                                                                                                                     <  1:4243701/150‑1 (MQ=255)

                                 ttCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGc                                                                                                                    >  2:969030/1‑150 (MQ=255)

                                   cGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGa                                                                                                                  <  1:870889/150‑1 (MQ=255)

                                    gggCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGaa                                                                                                                 <  2:1369614/150‑1 (MQ=255)

                                    gggCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGaa                                                                                                                 <  1:1649450/150‑1 (MQ=255)

                                      gcgcCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAaca                                                                                                               >  2:1693597/1‑150 (MQ=255)

                                           ttGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACaccgaa                                                                                                          >  1:2904735/1‑150 (MQ=255)

                                           ttGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACaccgaa                                                                                                          >  2:2943365/1‑150 (MQ=255)

                                            tGGCGGCAATCAGGTGATAGGACACCACTCAGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACaccgaac                                                                                                         >  1:2763142/1‑150 (MQ=255)

                                               cggcAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACaccgaaccga                                                                                                      <  1:792952/150‑1 (MQ=255)

                                                        ggTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACa                                                                                             >  2:4462846/1‑150 (MQ=255)

                                                            aTAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGaa                                                                                         <  1:2722545/150‑1 (MQ=255)

                                                            aTAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGaa                                                                                         <  1:3759900/150‑1 (MQ=255)

                                                            aTAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGaa                                                                                         >  1:2090475/1‑150 (MQ=255)

                                                              aGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGt                                                                                       <  2:3504642/150‑1 (MQ=255)

                                                                gCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGttt                                                                                     >  2:4300082/1‑150 (MQ=255)

                                                                gCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGttt                                                                                     >  1:4384612/1‑150 (MQ=255)

                                                                  caccacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCCTTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGacggtgtc                                                                                   >  2:3800429/1‑143 (MQ=255)

                                                                  caccacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTc                                                                                   <  2:2534198/150‑1 (MQ=255)

                                                                  caccacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTc                                                                                   <  1:1332352/150‑1 (MQ=255)

                                                                  caccacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTc                                                                                   <  1:1022379/150‑1 (MQ=255)

                                                                  caccacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTc                                                                                   >  2:1524560/1‑150 (MQ=255)

                                                                  caccacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTc                                                                                   <  2:1307291/150‑1 (MQ=255)

                                                                   accacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCg                                                                                  <  2:2663837/150‑1 (MQ=255)

                                                                   accacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCg                                                                                  >  2:1243840/1‑150 (MQ=255)

                                                                     cacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTa                                                                                >  1:962811/1‑150 (MQ=255)

                                                                       cTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACt                                                                              <  1:4378617/150‑1 (MQ=255)

                                                                       cTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACt                                                                              <  1:1273106/150‑1 (MQ=255)

                                                                           ggTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGc                                                                          >  1:23540/1‑150 (MQ=255)

                                                                           ggTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGc                                                                          <  1:3719497/150‑1 (MQ=255)

                                                                                  catgCCACCAACGCCTGCCAGTGCAATCCATTGACCTGTGCTGGCAGACGGCGTCGACTGACCACTTAGTTGTCCATCAGCACCACGCCTAACAGCGGTGCGAACACCGAACCGATCAGAAACAGGAAGTTTTCGTACTGTGCCAGTGgc                                                                   <  1:1993397/147‑1 (MQ=255)

                                                                                  cAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGCACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGgc                                                                   <  2:2416668/150‑1 (MQ=255)

                                                                                  cAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGgc                                                                   >  1:1048134/1‑150 (MQ=255)

                                                                                  cAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGgc                                                                   >  2:1447932/1‑150 (MQ=255)

                                                                                       caccaACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCaa                                                              >  2:3423310/1‑150 (MQ=255)

                                                                                       caccaACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCaa                                                              >  2:2480114/1‑150 (MQ=255)

                                                                                         ccaACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACa                                                            <  1:3551171/150‑1 (MQ=255)

                                                                                          caACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAg                                                           >  2:2726030/1‑150 (MQ=255)

                                                                                          caACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAg                                                           >  2:746138/1‑150 (MQ=255)

                                                                                          caACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTGTTCGTACTGTGCCAGTGGCGCCAACAg                                                           >  1:4076892/1‑150 (MQ=255)

                                                                                           aaCGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAgg                                                          <  1:719762/150‑1 (MQ=255)

                                                                                            aCGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAggg                                                         <  2:3636740/150‑1 (MQ=255)

                                                                                            aCGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAggg                                                         <  1:4259978/150‑1 (MQ=255)

                                                                                                  gCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCa                                                   <  2:555862/150‑1 (MQ=255)

                                                                                                    cAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAgg                                                 >  2:1929340/1‑150 (MQ=255)

                                                                                                     aGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAggg                                                >  1:2486972/1‑150 (MQ=255)

                                                                                                      gTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGt                                               <  2:227851/150‑1 (MQ=255)

                                                                                                      gTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGt                                               <  2:2166759/150‑1 (MQ=255)

                                                                                                      gTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGt                                               <  2:3314227/150‑1 (MQ=255)

                                                                                                      gTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGt                                               <  1:2640093/150‑1 (MQ=255)

                                                                                                      gTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGt                                               <  1:1628123/150‑1 (MQ=255)

                                                                                                      gTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGt                                               <  2:3226548/150‑1 (MQ=255)

                                                                                                       tGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTg                                              >  1:3868417/1‑150 (MQ=255)

                                                                                                       tGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTg                                              <  2:1841829/150‑1 (MQ=255)

                                                                                                       tGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTg                                              <  1:3309931/150‑1 (MQ=255)

                                                                                                          aaGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGcac                                           >  1:4006627/1‑150 (MQ=255)

                                                                                                           aGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGcaca                                          >  2:3281252/1‑150 (MQ=255)

                                                                                                            gCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCacaa                                         >  1:217897/1‑150 (MQ=255)

                                                                                                                ttGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACg                                     >  2:318565/1‑150 (MQ=255)

                                                                                                                 tGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGt                                                                                                                      <  2:840606/68‑1 (MQ=255)

                                                                                                                   aCCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCg                                  >  1:2555035/1‑150 (MQ=255)

                                                                                                                      tgtgCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATg                               <  2:1790151/150‑1 (MQ=255)

                                                                                                                               aGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGcc                      <  1:1800852/150‑1 (MQ=255)

                                                                                                                                gACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCa                     >  1:3188456/1‑150 (MQ=255)

                                                                                                                                gACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCa                     <  1:1966922/150‑1 (MQ=255)

                                                                                                                                      gTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCt               <  1:4462952/150‑1 (MQ=255)

                                                                                                                                          atggACCACTACGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGAc           >  1:3782635/3‑150 (MQ=255)

                                                                                                                                          aCGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGAc           >  1:849594/1‑150 (MQ=255)

                                                                                                                                           cGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGAcc          <  2:72476/150‑1 (MQ=255)

                                                                                                                                            ggACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCt         >  2:523717/1‑150 (MQ=255)

                                                                                                                                               ccACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCa      <  1:3983938/150‑1 (MQ=255)

                                                                                                                                               ccACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCa      <  2:1622660/150‑1 (MQ=255)

                                                                                                                                                 aCGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGc    <  2:1171788/150‑1 (MQ=255)

                                                                                                                                                 aCGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGc    >  1:1593455/1‑150 (MQ=255)

                                                                                                                                                 aCGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGc    <  2:3849899/150‑1 (MQ=255)

                                                                                                                                                   gTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGCgg  <  1:1524560/150‑1 (MQ=255)

TGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGCGG  >  NC_002947/5594800‑5595096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`