BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,594,948	T→A	5.1%	Y360F (TAC→TTC) ‡	PP_4921 ←	NCS1 family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,594,948	0	T	A	5.1%	936.9 / 28.0	272	Y360F (TAC→TTC) ‡	PP_4921	NCS1 family transporter
Reads supporting (aligned to +/- strand): ref base T (106/151); new base A (5/9); total (112/160)
Fisher's exact test for biased strand distribution p-value = 7.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 273 total aligned reads displayed.

TGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGCGGT  >  NC_002947/5594800‑5595097

tGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTa                                                                                                                                                      <  2:3257276/150‑1 (MQ=255)

tGCAAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTa                                                                                                                                                      >  2:2341991/1‑150 (MQ=255)

ggCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTa                                                                                                                                                      <  2:263786/149‑1 (MQ=255)

   cagcagCTATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTg                                                                                                                                                   <  2:1670199/150‑1 (MQ=255)

   cagcagCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTg                                                                                                                                                   <  2:3081300/150‑1 (MQ=255)

   cagcagCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTg                                                                                                                                                   <  2:4390344/150‑1 (MQ=255)

    agcagcAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTgg                                                                                                                                                  >  2:3555087/1‑150 (MQ=255)

       agcaATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTcc                                                                                                                                               >  1:3636740/1‑150 (MQ=255)

       agcaATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTcc                                                                                                                                               >  1:731409/1‑150 (MQ=255)

        gcaATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCa                                                                                                                                              >  2:4259978/1‑150 (MQ=255)

        gcaATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCa                                                                                                                                              <  1:4349946/150‑1 (MQ=255)

         caATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACTACGTGGTccgt                                                                                                                                             <  1:3537887/150‑3 (MQ=255)

          aaTGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCGAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATc                                                                                                                                            >  1:1171788/1‑150 (MQ=255)

          aaTGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGATGGACCACTACGTGGTCCGTc                                                                                                                                            <  1:3771750/150‑1 (MQ=255)

           aTGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCa                                                                                                                                           >  1:1790151/1‑150 (MQ=255)

           aTGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCa                                                                                                                                           >  1:479209/1‑150 (MQ=255)

             gCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGATGGACCACTACGTGGTCCGTcgac                                                                                                                                         <  1:2018844/150‑4 (MQ=255)

             gCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGATGGACCACTACGTGGTCCGTcgac                                                                                                                                         <  1:922128/150‑4 (MQ=255)

               gggCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGcac                                                                                                                                       >  1:3849768/1‑150 (MQ=255)

                       tGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGa                                                                                                                               >  1:1822033/1‑150 (MQ=255)

                       tGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGa                                                                                                                               <  1:3439548/150‑1 (MQ=255)

                        ggCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACAGACCACGTAGTGGTCCATCAGCACCACGCCGaa                                                                                                                              <  2:1322439/150‑1 (MQ=255)

                            cccAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGc                                                                                                                          >  1:1432768/1‑150 (MQ=255)

                               aGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGt                                                                                                                       <  1:3689811/150‑1 (MQ=255)

                                gTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTg                                                                                                                      <  1:4243728/150‑1 (MQ=255)

                                 ttCGGGCGCCTTGGCGGCAATCAGGTGCTAGGCCACCACTCCTGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGc                                                                                                                     <  1:674337/150‑1 (MQ=255)

                                 ttCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGc                                                                                                                     <  2:4112889/150‑1 (MQ=255)

                                 ttCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGc                                                                                                                     >  2:969030/1‑150 (MQ=255)

                                    gggCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGaa                                                                                                                  <  2:1369614/150‑1 (MQ=255)

                                    gggCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGaa                                                                                                                  <  1:1649450/150‑1 (MQ=255)

                                        gcCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACacc                                                                                                              >  1:3984445/1‑150 (MQ=255)

                                           ttGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACaccgaa                                                                                                           >  1:2904735/1‑150 (MQ=255)

                                           ttGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACaccgaa                                                                                                           >  2:2943365/1‑150 (MQ=255)

                                            tGGCGGCAATCAGGTGATAGGACACCACTCAGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACaccgaac                                                                                                          >  1:2763142/1‑150 (MQ=255)

                                               cggcAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACaccgaaccga                                                                                                       <  1:792952/150‑1 (MQ=255)

                                                 gcAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATc                                                                                                     <  2:3324570/150‑1 (MQ=255)

                                                        ggTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACa                                                                                              >  2:4462952/1‑150 (MQ=255)

                                                        ggTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACa                                                                                              >  2:155392/1‑150 (MQ=255)

                                                          tGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAgg                                                                                            >  2:2092416/1‑150 (MQ=255)

                                                          tGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAgg                                                                                            <  2:3213864/150‑1 (MQ=255)

                                                            aTAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGaa                                                                                          >  1:2090475/1‑150 (MQ=255)

                                                             tAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAg                                                                                         <  2:2764118/150‑1 (MQ=255)

                                                                gCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGttt                                                                                      >  2:1261616/1‑150 (MQ=255)

                                                                  caccacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTc                                                                                    <  2:2534198/150‑1 (MQ=255)

                                                                  caccacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTc                                                                                    <  1:1332352/150‑1 (MQ=255)

                                                                  caccacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTc                                                                                    <  1:1022379/150‑1 (MQ=255)

                                                                     cacTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTa                                                                                 >  1:962811/1‑150 (MQ=255)

                                                                       cTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACt                                                                               <  1:1273106/150‑1 (MQ=255)

                                                                           ggTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGc                                                                           <  1:3719497/150‑1 (MQ=255)

                                                                           ggTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGc                                                                           <  1:1816650/150‑1 (MQ=255)

                                                                             tAAACCAGGCCCCCACCGCCTGCCAGTGCAAGCCATCGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCa                                                                         <  2:2505044/150‑1 (MQ=255)

                                                                               aaCCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGt                                                                       <  1:4434037/150‑1 (MQ=255)

                                                                                  catgCCACCAACGCCTGCCAGTGCAATCCATTGACCTGTGCTGGCAGACGGCGTCGACTGACCACTTAGTTGTCCATCAGCACCACGCCTAACAGCGGTGCGAACACCGAACCGATCAGAAACAGGAAGTTTTCGTACTGTGCCAGTGgc                                                                    <  1:1993397/147‑1 (MQ=255)

                                                                                  cAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGgc                                                                    >  2:1447932/1‑150 (MQ=255)

                                                                                  cAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGgc                                                                    >  1:1048134/1‑150 (MQ=255)

                                                                                     gCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGcc                                                                 >  2:4030953/1‑150 (MQ=255)

                                                                                       caccaACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCaa                                                               >  2:3423310/1‑150 (MQ=255)

                                                                                        accaACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAAc                                                              >  1:4380942/1‑150 (MQ=255)

                                                                                          caACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAg                                                            >  2:746138/1‑150 (MQ=255)

                                                                                          caACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAg                                                            >  1:2390696/1‑150 (MQ=255)

                                                                                          caACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTGTTCGTACTGTGCCAGTGGCGCCAACAg                                                            >  1:4076892/1‑150 (MQ=255)

                                                                                            aCGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAggg                                                          <  1:4259978/150‑1 (MQ=255)

                                                                                                  gCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCa                                                    >  2:3636791/1‑150 (MQ=255)

                                                                                                  gCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCa                                                    <  2:555862/150‑1 (MQ=255)

                                                                                                    cAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAgg                                                  <  2:1799793/150‑1 (MQ=255)

                                                                                                    cAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAgg                                                  >  2:1929340/1‑150 (MQ=255)

                                                                                                     aGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAggg                                                 >  1:2486972/1‑150 (MQ=255)

                                                                                                      gTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGt                                                <  2:2166759/150‑1 (MQ=255)

                                                                                                      gTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGt                                                <  2:3226548/150‑1 (MQ=255)

                                                                                                      gTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGt                                                <  1:2640093/150‑1 (MQ=255)

                                                                                                      gTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGt                                                <  1:1628123/150‑1 (MQ=255)

                                                                                                       tGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTg                                               <  2:1841829/150‑1 (MQ=255)

                                                                                                       tGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTg                                               <  1:3309931/150‑1 (MQ=255)

                                                                                                        gCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGc                                              <  2:2418186/150‑1 (MQ=255)

                                                                                                          aaGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGcac                                            >  1:4006627/1‑150 (MQ=255)

                                                                                                          aaGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGcac                                            >  2:1696492/1‑150 (MQ=255)

                                                                                                           aGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGcaca                                           >  2:3281319/1‑150 (MQ=255)

                                                                                                            gCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCacaa                                          >  1:217897/1‑150 (MQ=255)

                                                                                                                 tGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGt                                                                                                                       <  2:840606/68‑1 (MQ=255)

                                                                                                                   aCCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCg                                   >  1:2555035/1‑150 (MQ=255)

                                                                                                                   aCCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCg                                   >  2:2718432/1‑150 (MQ=255)

                                                                                                                     cTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGAt                                 >  2:4294176/1‑150 (MQ=255)

                                                                                                                                gACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCa                      <  1:2727054/150‑1 (MQ=255)

                                                                                                                                gACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCa                      >  1:3188456/1‑150 (MQ=255)

                                                                                                                                gACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCa                      <  1:3974184/150‑1 (MQ=255)

                                                                                                                                gACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCa                      <  1:1966922/150‑1 (MQ=255)

                                                                                                                                 aCGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAg                     >  1:3581353/1‑150 (MQ=255)

                                                                                                                                 aCGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAg                     <  2:39034/150‑1 (MQ=255)

                                                                                                                                      gTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCt                <  1:4462952/150‑1 (MQ=255)

                                                                                                                                         gACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGa             >  2:2039193/1‑150 (MQ=255)

                                                                                                                                          atggACCACTACGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGAc            >  1:3782635/3‑150 (MQ=255)

                                                                                                                                          aCGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGAc            >  1:849594/1‑150 (MQ=255)

                                                                                                                                           cGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGAcc           <  2:72476/150‑1 (MQ=255)

                                                                                                                                            ggACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCt          >  2:523717/1‑150 (MQ=255)

                                                                                                                                               ccACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCa       <  2:1622661/150‑1 (MQ=255)

                                                                                                                                                 aCGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGc     >  1:1593455/1‑150 (MQ=255)

                                                                                                                                                 aCGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGc     <  2:3849768/150‑1 (MQ=255)

                                                                                                                                                 aCGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGc     <  2:1171788/150‑1 (MQ=255)

                                                                                                                                                   gTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGCgg   <  1:1524560/150‑1 (MQ=255)

                                                                                                                                                    tAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGCGGt  <  2:479209/150‑1 (MQ=255)

TGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAACACCGAACCGATCAGCAACAGGAAGTTTTCGTACTGTGCCAGTGGCGCCAACAGGGCGATCAGGGTGCACAACACGCCGATGGCCAACGCCAGGTGCTCGACCTTCAGCGGT  >  NC_002947/5594800‑5595097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`