Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,708,097 A→G 6.8% H46R (CAC→CGC) ‡ PP_5009 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,708,0970AG 6.8% 657.1 / 24.1 205H46R (CAC→CGC) ‡PP_5009hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (109/82);  new base G (5/9);  total (114/91)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

Only 100 of 209 total aligned reads displayed.

GGACATCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAG  >  NC_002947/5707948‑5708246
                                                                                                                                                     |                                                                                                                                                     
    aTCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGCTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGc                                                                                                                                                   <  1:3808430/150‑1 (MQ=255)
    aTCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTAAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACTTCTTATCAAAAGAAGCATTTCTGGTTTTCAGCGACCGGTATCCATGGTTGCACCGc                                                                                                                                                   <  1:2842931/150‑1 (MQ=255)
     tCGACGCCATGACTGCTGTAGCACACCCACCCAGGCTGACGTTCAAGCAATGCGTGGCAGCCTGTTTCGCCAAACGACCCAGCCTGAGGGACGTCTGATCACCAGAAGCATTTCAGGGTTTCAGCGACCGGTAGCCATGGTTGCACCGcc                                                                                                                                                  >  2:3574536/1‑149 (MQ=255)
          gCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccat                                                                                                                                             <  2:892647/150‑2 (MQ=255)
          gCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccat                                                                                                                                             <  2:1565877/150‑2 (MQ=255)
           ccATGACTGCTGTAGCAAACCCCCCCCGGCTGACGTTCAAGCACAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccatg                                                                                                                                            <  2:1264204/150‑3 (MQ=255)
           ccATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTATTCAGCGACCGGTATCCATGGTTGCGGTGCAaccatg                                                                                                                                            <  2:2220155/150‑3 (MQ=255)
            cATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCAccc                                                                                                                                           >  1:2157664/1‑150 (MQ=255)
             aTGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCt                                                                                                                                          >  1:829383/1‑150 (MQ=255)
             aTGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCt                                                                                                                                          >  1:4189530/1‑150 (MQ=255)
             aTGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCt                                                                                                                                          >  1:4317392/1‑150 (MQ=255)
             aTGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCt                                                                                                                                          >  2:327194/1‑150 (MQ=255)
              tGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtc                                                                                                                                         <  2:4232855/150‑1 (MQ=255)
               gACTGCTGTAGCAAACCCACCCCGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccatggata                                                                                                                                        <  2:948070/150‑7 (MQ=255)
               gACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtca                                                                                                                                        <  1:1717382/150‑1 (MQ=255)
                  tgctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacat                                                                                                                                     >  2:2101332/1‑150 (MQ=255)
                  tgctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacat                                                                                                                                     >  2:4037984/1‑150 (MQ=255)
                  tgctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacat                                                                                                                                     >  1:3291629/1‑150 (MQ=255)
                       tAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacatcacag                                                                                                                                >  2:47803/1‑149 (MQ=255)
                       tAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTcacatcacat                                                                                                                                >  1:247247/1‑150 (MQ=255)
                       tAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTcacatcacat                                                                                                                                >  1:3446824/1‑150 (MQ=255)
                       tAGAAAACCCACCCAGGCTGACGTTAAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTcacatcacat                                                                                                                                <  1:864293/150‑1 (MQ=255)
                        aGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGTTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCacatcacatc                                                                                                                               <  1:4091489/150‑1 (MQ=255)
                         gCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCg                                                                                                                              >  2:1176307/1‑150 (MQ=255)
                           aaaCCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCt                                                                                                                            <  1:2750883/150‑1 (MQ=255)
                           aaaCCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCt                                                                                                                            >  2:3356400/1‑150 (MQ=255)
                             acccacccAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCg                                                                                                                          >  2:559346/1‑150 (MQ=255)
                               ccacccaGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGAt                                                                                                                        >  1:4439700/1‑150 (MQ=255)
                                   ccaGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCtt                                                                                                                    >  2:1673436/1‑150 (MQ=255)
                                     aGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTc                                                                                                                  <  2:3386059/150‑1 (MQ=255)
                                     aGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTc                                                                                                                  <  2:236058/150‑1 (MQ=255)
                                     aGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTc                                                                                                                  <  1:253487/150‑1 (MQ=255)
                                     aGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTc                                                                                                                  <  2:635504/150‑1 (MQ=255)
                                         tGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCa                                                                                                              >  2:2961727/1‑150 (MQ=255)
                                                 aaGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCAt                                                                                                      >  1:3462143/1‑150 (MQ=255)
                                                 aaGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCAt                                                                                                      >  1:2884489/1‑150 (MQ=255)
                                                         cGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGc                                                                                              <  1:351314/150‑1 (MQ=255)
                                                         cGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGc                                                                                              <  2:2006698/150‑1 (MQ=255)
                                                          gTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGcc                                                                                             <  2:1636720/150‑1 (MQ=255)
                                                           tGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCa                                                                                            <  1:369017/150‑1 (MQ=255)
                                                           tGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCa                                                                                            <  2:1094419/150‑1 (MQ=255)
                                                              cAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGca                                                                                         >  2:1816837/1‑150 (MQ=255)
                                                               aGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCAAGGTTGCACCGCAACCACCCTCACATCACATCGCACGATCCTTTCGTCATCCTGCATGAACCGGCCAGcac                                                                                        >  2:2090920/1‑150 (MQ=255)
                                                               aGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCAGTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGcac                                                                                        >  2:2266971/1‑150 (MQ=255)
                                                                  cTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAgg                                                                                     >  1:301151/1‑150 (MQ=255)
                                                                     tttCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCt                                                                                                                            <  1:3694748/108‑1 (MQ=255)
                                                                     tttCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCt                                                                                                                            >  2:3694748/1‑108 (MQ=255)
                                                                      ttCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGAt                                                                                 <  1:2805072/150‑1 (MQ=255)
                                                                         tcCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGAtctc                                                                              >  1:1024613/1‑150 (MQ=255)
                                                                         tcCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGAtctc                                                                              >  1:3209209/1‑150 (MQ=255)
                                                                          ccAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTcc                                                                             <  2:1029382/150‑1 (MQ=255)
                                                                           cAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTccc                                                                            <  1:240196/150‑1 (MQ=255)
                                                                           cAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTccc                                                                            >  2:4450528/1‑150 (MQ=255)
                                                                            aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACAACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCa                                                                           <  2:3894928/150‑1 (MQ=255)
                                                                             aaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAg                                                                          <  1:1885652/150‑1 (MQ=255)
                                                                               cGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGt                                                                        <  2:2753173/150‑1 (MQ=255)
                                                                                   ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                    >  2:3814560/1‑150 (MQ=255)
                                                                                   ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                    >  2:3050413/1‑150 (MQ=255)
                                                                                   ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                    >  2:1091335/1‑150 (MQ=255)
                                                                                        cTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCggg                                                               <  1:415730/150‑1 (MQ=255)
                                                                                         tGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgc                                                              <  2:1106569/150‑1 (MQ=255)
                                                                                         tGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgc                                                              >  1:4215159/1‑150 (MQ=255)
                                                                                         tGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgc                                                              >  1:739632/1‑150 (MQ=255)
                                                                                             ggACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGTgctgat                                                          <  2:192480/150‑3 (MQ=255)
                                                                                             ggACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgctgct                                                          <  2:192047/150‑1 (MQ=255)
                                                                                             ggACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgctgct                                                          >  2:454000/1‑150 (MQ=255)
                                                                                             ggACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgctgct                                                          >  2:2924777/1‑150 (MQ=255)
                                                                                              gACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGctgctg                                                         <  1:687256/150‑1 (MQ=255)
                                                                                                cGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGa                                                       >  1:796065/1‑150 (MQ=255)
                                                                                                cGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTATCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGa                                                       >  1:1448990/1‑150 (MQ=255)
                                                                                                      aTCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCt                                                 >  2:2415024/1‑150 (MQ=255)
                                                                                                       tCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCtt                                                <  1:2905594/150‑1 (MQ=255)
                                                                                                        cAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTtc                                               >  1:2797396/1‑150 (MQ=255)
                                                                                                           aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                            >  2:1814118/1‑150 (MQ=255)
                                                                                                           aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                            <  1:4463084/150‑1 (MQ=255)
                                                                                                            agaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAg                                           >  1:4484520/1‑150 (MQ=255)
                                                                                                              aagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGc                                         >  2:782194/1‑150 (MQ=255)
                                                                                                                   tttCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCAtttt                                    >  2:3977051/1‑150 (MQ=255)
                                                                                                                     tCAGTTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCt                                  <  2:824672/150‑1 (MQ=255)
                                                                                                                     tCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCt                                  <  1:1519789/150‑1 (MQ=255)
                                                                                                                     tCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCt                                  <  2:2176300/150‑1 (MQ=255)
                                                                                                                      cAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTg                                 <  1:310150/150‑1 (MQ=255)
                                                                                                                       aGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTgg                                >  1:590416/1‑150 (MQ=255)
                                                                                                                           tttCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCaa                            <  1:559346/150‑1 (MQ=255)
                                                                                                                               aGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGaa                        >  1:1074534/1‑150 (MQ=255)
                                                                                                                                  gACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACgt                     <  1:2173676/150‑1 (MQ=255)
                                                                                                                                   aCCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACgtg                    <  2:247247/150‑1 (MQ=255)
                                                                                                                                          tCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACt             >  1:3981183/1‑150 (MQ=255)
                                                                                                                                           ccATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTa            >  1:1195326/1‑150 (MQ=255)
                                                                                                                                            cATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTaa           >  1:1653422/1‑150 (MQ=255)
                                                                                                                                            cATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTaa           >  2:4328965/1‑150 (MQ=255)
                                                                                                                                             gtGGTTGCGGTGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgctgct                                                          <  2:2503352/101‑1 (MQ=255)
                                                                                                                                             gtGGTTGCGGTGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg          >  1:5173/2‑150 (MQ=255)
                                                                                                                                             aTGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg          >  2:2803559/1‑150 (MQ=255)
                                                                                                                                              tGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAgc         <  2:1072014/150‑1 (MQ=255)
                                                                                                                                                 ttGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGcgcc      >  1:1964919/1‑150 (MQ=255)
                                                                                                                                                  tGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGcgccg     <  2:340294/150‑1 (MQ=255)
                                                                                                                                                   gCACCGCAACCACCCTCACCTCACATCGCTCGATCCTTTCGTCATCCGGCAAGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGcgccgc    <  2:4062597/150‑1 (MQ=255)
                                                                                                                                                   gCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGcgccgc    >  2:3282890/1‑150 (MQ=255)
                                                                                                                                                     aCCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAg  >  1:1576319/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGACATCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAG  >  NC_002947/5707948‑5708246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑