Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,708,099 C→T 6.8% R47C (CGC→TGC)  PP_5009 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,708,0990CT 6.8% 641.7 / 25.8 205R47C (CGC→TGC) PP_5009hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (111/79);  new base T (5/9);  total (116/89)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 206 total aligned reads displayed.

ACATCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAG  >  NC_002947/5707950‑5708246
                                                                                                                                                     |                                                                                                                                                   
  aTCGACGCCATGACTGCTGTAGCAACCCCACCCCGGCTGACGTTCAAGCAACGCGTGGCAGCCTGCTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGc                                                                                                                                                   <  1:3808072/150‑1 (MQ=255)
  aTCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGc                                                                                                                                                   >  2:369017/1‑150 (MQ=255)
  aTCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGc                                                                                                                                                   >  1:2365850/1‑150 (MQ=255)
  aTCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTAAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACTTCTTATCAAAAGAAGCATTTCTGGTTTTCAGCGACCGGTATCCATGGTTGCACCGc                                                                                                                                                   <  1:2842931/150‑1 (MQ=255)
   tCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCa                                                                                                                                                  >  2:3574247/1‑150 (MQ=255)
     gACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTAGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAac                                                                                                                                                <  2:932584/150‑1 (MQ=255)
      aCGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAacc                                                                                                                                               <  2:171757/150‑1 (MQ=255)
        gCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccat                                                                                                                                             <  2:1565877/150‑2 (MQ=255)
         ccATGACTGCTGTAGCAAACCCCCCCCGGCTGACGTTCAAGCACAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccatg                                                                                                                                            <  2:1264204/150‑3 (MQ=255)
         ccATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccatg                                                                                                                                            <  1:821215/150‑3 (MQ=255)
          cATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCAccc                                                                                                                                           >  1:2157664/1‑150 (MQ=255)
           aTGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCt                                                                                                                                          >  1:829430/1‑150 (MQ=255)
           aTGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCt                                                                                                                                          >  2:327194/1‑150 (MQ=255)
             gACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtca                                                                                                                                        <  1:1717382/150‑1 (MQ=255)
                tgctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacat                                                                                                                                     >  1:2421303/1‑150 (MQ=255)
                tgctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacat                                                                                                                                     >  2:4037984/1‑150 (MQ=255)
                   tgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacatcac                                                                                                                                  >  2:2411348/1‑150 (MQ=255)
                     tAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTcacatcacat                                                                                                                                <  2:490523/150‑1 (MQ=255)
                       gCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCg                                                                                                                              >  2:1176307/1‑150 (MQ=255)
                       gCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCg                                                                                                                              >  1:95591/1‑150 (MQ=255)
                         aaaCCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCt                                                                                                                            <  1:496155/150‑1 (MQ=255)
                          aaCCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTc                                                                                                                           >  2:1635797/1‑150 (MQ=255)
                             ccacccaGGCTGACGTTCAATCAAAGCGTGGCAGCCTTTTTCTCCAAACGACCAAGCCTGAGGGACGTATTATCAAAAGAAGAATTTCAGTTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGAt                                                                                                                        <  1:3562100/150‑1 (MQ=255)
                             ccacccaGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGAt                                                                                                                        >  1:3911084/1‑150 (MQ=255)
                             ccacccaGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGAt                                                                                                                        >  2:2267666/1‑150 (MQ=255)
                             ccacccaGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGAt                                                                                                                        <  1:3165334/150‑1 (MQ=255)
                               acccaGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGCTcc                                                                                                                      >  1:4402533/1‑150 (MQ=255)
                                 ccaGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCtt                                                                                                                    >  2:413225/1‑150 (MQ=255)
                                 ccaGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCtt                                                                                                                    >  1:340294/1‑150 (MQ=255)
                                   aGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTc                                                                                                                  <  2:1059438/150‑1 (MQ=255)
                                       tGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCa                                                                                                              >  2:2961727/1‑150 (MQ=255)
                                        gACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCAt                                                                                                             <  2:2468885/150‑1 (MQ=255)
                                            ttCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTg                                                                                                         >  1:4164225/1‑150 (MQ=255)
                                               aaGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCAt                                                                                                      >  1:2884489/1‑150 (MQ=255)
                                                   aaaGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAAc                                                                                                  >  1:4210538/1‑150 (MQ=255)
                                                       cGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGc                                                                                              <  2:2006944/150‑1 (MQ=255)
                                                        gTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGcc                                                                                             <  2:580456/150‑1 (MQ=255)
                                                         tGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCa                                                                                            <  2:1094419/150‑1 (MQ=255)
                                                           gCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGc                                                                                          >  1:2620544/1‑150 (MQ=255)
                                                            cAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGca                                                                                         >  2:1816699/1‑150 (MQ=255)
                                                             aGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGcac                                                                                        >  2:2091260/1‑150 (MQ=255)
                                                                cTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAgg                                                                                     >  1:301151/1‑150 (MQ=255)
                                                                cTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAgg                                                                                     <  2:4337233/150‑1 (MQ=255)
                                                                    ttCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGAt                                                                                 <  1:2805072/150‑1 (MQ=255)
                                                                       tcCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGAtctc                                                                              >  1:4481108/1‑150 (MQ=255)
                                                                       tcCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGAtctc                                                                              >  1:1024613/1‑150 (MQ=255)
                                                                          aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCa                                                                           >  2:3398868/1‑150 (MQ=255)
                                                                          aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACAACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCa                                                                           <  2:3894928/150‑1 (MQ=255)
                                                                           aaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAg                                                                          <  1:1885652/150‑1 (MQ=255)
                                                                             cGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGt                                                                        <  2:2753173/150‑1 (MQ=255)
                                                                                 ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                    >  2:3814560/1‑150 (MQ=255)
                                                                                 ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                    >  2:3050413/1‑150 (MQ=255)
                                                                                 ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                    >  2:1091335/1‑150 (MQ=255)
                                                                                    gCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCg                                                                 <  2:4301635/150‑1 (MQ=255)
                                                                                       tGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgc                                                              <  2:1106569/150‑1 (MQ=255)
                                                                                       tGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgc                                                              >  1:739632/1‑150 (MQ=255)
                                                                                           ggACGTCTTATCAAACGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCGCACATCACCTCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgctgct                                                          <  2:792924/150‑1 (MQ=255)
                                                                                           ggACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgctgct                                                          >  2:2924777/1‑150 (MQ=255)
                                                                                           ggACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgctgct                                                          <  2:3285428/150‑1 (MQ=255)
                                                                                            gACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGctgctg                                                         <  1:687256/150‑1 (MQ=255)
                                                                                              cGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTATCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGa                                                       >  1:1448990/1‑150 (MQ=255)
                                                                                                 cTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGa                                                    <  1:3461419/150‑1 (MQ=255)
                                                                                                 cTTAACCAAAGACGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCCGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGa                                                    <  1:4147203/150‑1 (MQ=255)
                                                                                                     tCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCtt                                                >  2:455780/1‑150 (MQ=255)
                                                                                                     tCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCtt                                                >  2:827966/1‑150 (MQ=255)
                                                                                                     tCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCtt                                                >  2:3370582/1‑150 (MQ=255)
                                                                                                     tCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCtt                                                <  1:2905594/150‑1 (MQ=255)
                                                                                                        aaaGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTtctc                                             >  1:2782106/1‑150 (MQ=255)
                                                                                                         aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                            <  1:4462897/150‑1 (MQ=255)
                                                                                                         aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                            <  2:4481108/150‑1 (MQ=255)
                                                                                                         aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                            <  2:1906838/150‑1 (MQ=255)
                                                                                                          agaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAg                                           <  2:2257860/150‑1 (MQ=255)
                                                                                                            aagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACGACCCTCACCTCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGc                                         <  2:669891/150‑1 (MQ=255)
                                                                                                                 tttCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCAtttt                                    >  2:3977051/1‑150 (MQ=255)
                                                                                                                 tttCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCAtttt                                    >  2:1672656/1‑150 (MQ=255)
                                                                                                                  ttCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTc                                   >  1:4270366/1‑150 (MQ=255)
                                                                                                                   tCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCt                                  <  1:1519789/150‑1 (MQ=255)
                                                                                                                    cAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTg                                 <  1:310150/150‑1 (MQ=255)
                                                                                                                    cAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTg                                 >  2:1922216/1‑150 (MQ=255)
                                                                                                                     aGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTgg                                >  1:590416/1‑150 (MQ=255)
                                                                                                                       gTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGcc                              >  1:3254248/1‑150 (MQ=255)
                                                                                                                         tttCAGCGACCTTTATCCATGGTTGCACAGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCAGCATGAACCGGCCAGCAAAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCaa                            <  2:2187588/150‑1 (MQ=255)
                                                                                                                         tttCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCaa                            <  1:4037120/150‑1 (MQ=255)
                                                                                                                         tttCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCaa                            <  1:559346/150‑1 (MQ=255)
                                                                                                                          ttCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAg                           >  1:3494981/1‑150 (MQ=255)
                                                                                                                             aGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGaa                        >  1:1074534/1‑150 (MQ=255)
                                                                                                                                gACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACgt                     <  1:2173676/150‑1 (MQ=255)
                                                                                                                                    ggTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGc                 >  2:1958287/1‑150 (MQ=255)
                                                                                                                                         ccATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTa            >  1:1195326/1‑150 (MQ=255)
                                                                                                                                          cATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTaa           >  1:1653422/1‑150 (MQ=255)
                                                                                                                                          cATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTaa           >  1:369110/1‑150 (MQ=255)
                                                                                                                                           gtGGTTGCGGTGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgctgct                                                          >  1:2503352/2‑102 (MQ=255)
                                                                                                                                           gtGGTTGCGGTGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgctgct                                                          <  2:2503352/101‑1 (MQ=255)
                                                                                                                                           aTGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg          >  2:2803559/1‑150 (MQ=255)
                                                                                                                                           aTGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg          >  2:2140205/1‑150 (MQ=255)
                                                                                                                                            tGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAgc         >  1:767224/1‑150 (MQ=255)
                                                                                                                                               ttGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGcgcc      >  1:1964919/1‑150 (MQ=255)
                                                                                                                                                 gCACCGCAACCACCCTCACCTCACATCGCTCGATCCTTTCGTCATCCGGCAAGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGcgccgc    <  2:4062597/150‑1 (MQ=255)
                                                                                                                                                 gCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGcgccgc    >  2:3282806/1‑150 (MQ=255)
                                                                                                                                                   aCCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAg  >  1:1576319/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACATCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAG  >  NC_002947/5707950‑5708246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑