Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,858,755 C→T 5.3% A235V (GCC→GTC)  PP_5133 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,858,7550CT 5.3% 989.6 / 27.3 304A235V (GCC→GTC) PP_5133membrane protein
Reads supporting (aligned to +/- strand):  ref base C (170/117);  new base T (8/8);  total (179/125)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 310 total aligned reads displayed.

GCTGGCGATGCTCACCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGC  >  NC_002947/5858606‑5858904
                                                                                                                                                     |                                                                                                                                                     
 ctgGCGATGCTCACCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGcc                                                                                                                                                      <  1:410422/150‑1 (MQ=255)
  tgGCGATGCTCACCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCg                                                                                                                                                     >  2:1195003/1‑150 (MQ=255)
             aCCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGTCGGGCACGGCGaa                                                                                                                                          <  2:4403613/150‑1 (MQ=255)
             aCCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGTCGGGCACGGCGaa                                                                                                                                          <  2:2135670/150‑1 (MQ=255)
             aCCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGaa                                                                                                                                          <  2:1970538/150‑1 (MQ=255)
               cgcgCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGc                                                                                                                                        >  2:1157303/1‑150 (MQ=255)
                 cgcTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCa                                                                                                                                      <  1:2963996/150‑1 (MQ=255)
                  gcTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGTCGGGCACGGCGAAGccca                                                                                                                                     <  1:3650425/150‑3 (MQ=255)
                  gcTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCAt                                                                                                                                     <  1:1945771/150‑1 (MQ=255)
                             tACTGGTCATGAAGGAGACCCGGCTCTATGGCTTAGTCTGGTAAACCAACTTGCTCGCCTCCGACCCGTTCATACACCTGACCAAAGCACTGGGCGCCCTCCCGTCGTTGCTGTGATTCTCCGTGCCCGACGAAGCCATGATCAGCGCCa                                                                                                                          >  2:4174804/1‑150 (MQ=255)
                                   tCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAgcggcg                                                                                                                    >  1:3049559/1‑150 (MQ=255)
                                    cTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGa                                                                                                                   <  1:4260338/150‑1 (MQ=255)
                                     ttGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCCGCGGCGAc                                                                                                                  >  2:914168/1‑150 (MQ=255)
                                       gCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAg                                                                                                                <  2:2086504/150‑1 (MQ=255)
                                         tGGCGACCCGGCGCTATGGCGTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTGCATTCACCGGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGGGCCCGCCGAAGCCAGGATCCGCGCCAGCGGCGACAGcc                                                                                                              >  2:3839891/1‑150 (MQ=255)
                                          ggCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCt                                                                                                             <  1:3783413/150‑1 (MQ=255)
                                          ggCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCt                                                                                                             >  2:338551/1‑150 (MQ=255)
                                            cGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCAACAGCCTGc                                                                                                           <  2:3149155/150‑1 (MQ=255)
                                              aCCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCa                                                                                                         <  1:3897636/150‑1 (MQ=255)
                                              aCCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCa                                                                                                         <  1:2537434/150‑1 (MQ=255)
                                                ccGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGc                                                                                                       >  1:681207/1‑150 (MQ=255)
                                                  ggCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGccc                                                                                                     >  2:3601125/1‑150 (MQ=255)
                                                   gcgcTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCt                                                                                                    <  2:2768723/150‑1 (MQ=255)
                                                   gcgcTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCt                                                                                                    >  1:4109512/1‑150 (MQ=255)
                                                   gcgcTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCt                                                                                                    <  2:1784525/150‑1 (MQ=255)
                                                    cgcTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTg                                                                                                   <  1:2055678/150‑1 (MQ=255)
                                                      cTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGa                                                                                                 <  1:1540170/150‑1 (MQ=255)
                                                          ggCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTg                                                                                             <  1:2131284/150‑1 (MQ=255)
                                                           gCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTgg                                                                                            <  2:2862247/150‑1 (MQ=255)
                                                             tttGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGcc                                                                                          >  1:2206969/1‑150 (MQ=255)
                                                             tttGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGcc                                                                                          <  1:4213152/150‑1 (MQ=255)
                                                                  cTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGccctggc                                                                                     <  1:1641381/150‑5 (MQ=255)
                                                                  cTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCa                                                                                     <  2:148376/150‑1 (MQ=255)
                                                                   tGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAg                                                                                    >  2:2262393/1‑150 (MQ=255)
                                                                   tGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAg                                                                                    >  2:3031299/1‑150 (MQ=255)
                                                                   tGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAg                                                                                    >  1:261752/1‑150 (MQ=255)
                                                                   tGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAg                                                                                    >  2:4476264/1‑150 (MQ=255)
                                                                   tGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCAAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCAGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAg                                                                                    >  1:2697367/1‑150 (MQ=255)
                                                                    gggAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAgg                                                                                   >  1:3971491/1‑150 (MQ=255)
                                                                      gAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGcc                                                                                 >  2:3278584/1‑150 (MQ=255)
                                                                      gAAACCACCTTGCTCGCCGCCGACCCGAACATTCACCTGACCCAAGCACAGGGCGCCCTCACGTCGTAGCTGGGCTACGCCGTGCCCGACGAAGCCAAGATACCCGACACCCCCGACAGACTGCCAGCACAGGACGTGGCCCGCCAGGcc                                                                                 >  1:2909230/1‑150 (MQ=255)
                                                                         accaccTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTgg                                                                              <  1:2737829/150‑1 (MQ=255)
                                                                           caccTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGc                                                                            <  1:3704365/150‑1 (MQ=255)
                                                                           caccTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGc                                                                            >  1:1418093/1‑150 (MQ=255)
                                                                           caccTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGc                                                                            >  2:3339700/1‑150 (MQ=255)
                                                                             ccTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCa                                                                          <  2:1421280/150‑1 (MQ=255)
                                                                              cTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCac                                                                         <  1:1792749/150‑2 (MQ=255)
                                                                                tGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgct                                                                       <  1:515823/150‑1 (MQ=255)
                                                                                tGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgct                                                                       <  2:2570300/150‑1 (MQ=255)
                                                                                  cTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgctgg                                                                     <  2:4014547/150‑1 (MQ=255)
                                                                                  cTCGCCGCCGACCCGTTCAGTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgctgg                                                                     <  2:4239746/150‑1 (MQ=255)
                                                                                   tCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgctggc                                                                    >  1:658740/1‑150 (MQ=255)
                                                                                   tCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgctggc                                                                    >  2:2837091/1‑150 (MQ=255)
                                                                                    cgccgcCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCACCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgctggct                                                                   <  2:3126141/150‑1 (MQ=255)
                                                                                     gccgccGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgctggctg                                                                  <  2:2024722/150‑1 (MQ=255)
                                                                                         ccgACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCg                                                              >  1:3531885/1‑150 (MQ=255)
                                                                                           gACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGc                                                            >  1:174620/1‑150 (MQ=255)
                                                                                           gACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGAAGAAGACAATATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCAGGCTGCTCGGc                                                            >  1:871588/1‑150 (MQ=255)
                                                                                           gACCCGTTCATTCACCTGACCCAAGCACGGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGc                                                            <  2:3650706/150‑1 (MQ=255)
                                                                                              ccGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtg                                                         <  2:2632738/150‑1 (MQ=255)
                                                                                              ccGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtg                                                         >  2:3926568/1‑150 (MQ=255)
                                                                                               cGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtgg                                                        <  1:453228/150‑1 (MQ=255)
                                                                                                gTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATTATACGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtggt                                                       >  1:800727/1‑150 (MQ=255)
                                                                                                  tcattcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGc                                                     <  1:1165/150‑1 (MQ=255)
                                                                                                    attcaCCTGACCCACGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTg                                                   <  2:1921550/150‑1 (MQ=255)
                                                                                                    attcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTg                                                   >  1:1363490/1‑150 (MQ=255)
                                                                                                     ttcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTgg                                                  >  1:2452802/1‑150 (MQ=255)
                                                                                                      tcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGt                                                 <  1:1069803/150‑1 (MQ=255)
                                                                                                      tcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGt                                                 <  1:2851631/150‑1 (MQ=255)
                                                                                                        aCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGtat                                               >  1:4393701/1‑150 (MQ=255)
                                                                                                         ccTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGtata                                              >  1:3664674/1‑150 (MQ=255)
                                                                                                           tGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACg                                            >  1:1283319/1‑150 (MQ=255)
                                                                                                               ccAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCAGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCct                                        >  1:3142738/1‑150 (MQ=255)
                                                                                                               ccAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCGGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCct                                        >  1:2259676/1‑150 (MQ=255)
                                                                                                                cAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTCTACGGCctg                                       >  2:3519516/1‑150 (MQ=255)
                                                                                                                     aCTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGcc                                  >  1:1479213/1‑150 (MQ=255)
                                                                                                                       tGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGcccc                                >  2:1445201/1‑150 (MQ=255)
                                                                                                                       tGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGcccc                                <  2:3109406/150‑1 (MQ=255)
                                                                                                                          gcgcCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGcc                             >  2:2831778/1‑150 (MQ=255)
                                                                                                                          gcgcCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGGGCTGGTATACGGCCTGCTGCCCCGcc                             >  2:3463193/1‑150 (MQ=255)
                                                                                                                            gcCCTCCCGTCGTTGCTGGGCTTCGCCCTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctg                           >  1:1309904/1‑150 (MQ=255)
                                                                                                                               cTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctg                        <  2:1394764/150‑1 (MQ=255)
                                                                                                                               cTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctg                        >  2:2551486/1‑150 (MQ=255)
                                                                                                                               cTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctg                        <  1:2155412/150‑1 (MQ=255)
                                                                                                                                tCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctgc                       >  2:739888/1‑150 (MQ=255)
                                                                                                                                 cccGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctgct                      >  1:3272928/1‑150 (MQ=255)
                                                                                                                                  ccGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctgctg                     >  2:2649729/1‑150 (MQ=255)
                                                                                                                                      cgtTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCg                 <  2:584088/150‑1 (MQ=255)
                                                                                                                                         tGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGt              >  1:594184/1‑150 (MQ=255)
                                                                                                                                            tGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTAt           <  1:3422434/150‑1 (MQ=255)
                                                                                                                                            tGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCC‑GGTTATg          >  2:2352796/1‑150 (MQ=255)
                                                                                                                                             gggCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATg          >  2:1728138/1‑150 (MQ=255)
                                                                                                                                             gggCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATg          >  1:953512/1‑150 (MQ=255)
                                                                                                                                             gggCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATg          <  2:1288100/150‑1 (MQ=255)
                                                                                                                                                 ttCGTCGGGCACGGCGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCtg      >  2:4368468/1‑150 (MQ=255)
                                                                                                                                                 ttCGTCGGGCACGGCGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGCCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTCTGCCtg      >  2:4518186/1‑150 (MQ=255)
                                                                                                                                                 ttCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGccct      <  2:3885394/150‑3 (MQ=255)
                                                                                                                                                 ttCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCtg      <  2:1539022/150‑1 (MQ=255)
                                                                                                                                                   cGCCGTGCCCGACGACGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCtgtg    <  1:3839891/150‑1 (MQ=255)
                                                                                                                                                    gCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCTGtgg   <  1:3209793/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTGGCGATGCTCACCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGC  >  NC_002947/5858606‑5858904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑