BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,858,758	T→G	5.1%	V236G (GTG→GGG)	PP_5133 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,858,758	0	T	G	5.1%	1035.9 / 29.0	305	V236G (GTG→GGG)	PP_5133	membrane protein
Reads supporting (aligned to +/- strand): ref base T (168/120); new base G (9/8); total (177/128)
Fisher's exact test for biased strand distribution p-value = 8.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

GCGATGCTCACCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCT  >  NC_002947/5858610‑5858907

   aTGCTCACCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGccc                                                                                                                                                   <  1:2673824/150‑1 (MQ=255)

     gCTCACCGCGCTGGGCATGATACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAAACACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGACcga                                                                                                                                                 >  2:2294302/1‑150 (MQ=255)

      cTCACCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCcgac                                                                                                                                                >  1:3170480/1‑150 (MQ=255)

       tCACCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCcgacg                                                                                                                                               >  1:3236604/1‑150 (MQ=255)

         aCCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGTCGGGCACGGCGaa                                                                                                                                             <  2:2135644/150‑1 (MQ=255)

          ccGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGTCGGGCACGGCGAAg                                                                                                                                            <  1:2043645/150‑1 (MQ=255)

             cgcTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGGTTTGGCTTTGTCTGGGTAACCTCCTTGCTCGCCGCCGACCCGTTCATTCAGCTGACCCAAGCACTGGGCGCCCTCCCTTCGTTGCTGGGCTTCGCCGTGCCCGAGGAAGCCa                                                                                                                                         <  2:4206135/150‑1 (MQ=255)

             cgcTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCa                                                                                                                                         <  1:2963911/150‑1 (MQ=255)

              gcTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGTCGGGCACGGCGAAGccca                                                                                                                                        <  2:1639840/150‑3 (MQ=255)

                  ggCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATc                                                                                                                                    >  2:2569778/1‑150 (MQ=255)

                     aTGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCcgc                                                                                                                                 >  2:1405062/1‑150 (MQ=255)

                         tACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCa                                                                                                                             >  2:3946881/1‑150 (MQ=255)

                         tACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCa                                                                                                                             >  1:3698636/1‑150 (MQ=255)

                             ggTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAgcgg                                                                                                                         <  1:2784471/150‑1 (MQ=255)

                              gTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAgcggc                                                                                                                        >  2:2055908/1‑150 (MQ=255)

                               tCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAgcggcg                                                                                                                       >  1:3049559/1‑150 (MQ=255)

                                 ttGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCCGCGGCGAc                                                                                                                     >  2:914168/1‑150 (MQ=255)

                                 ttGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGAc                                                                                                                     >  2:3238713/1‑150 (MQ=255)

                                  tGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACa                                                                                                                    <  1:289887/150‑1 (MQ=255)

                                    cTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGc                                                                                                                  >  1:3898448/1‑150 (MQ=255)

                                        cGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGc                                                                                                              <  1:621376/150‑1 (MQ=255)

                                        cGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCAACAGCCTGc                                                                                                              <  2:3149121/150‑1 (MQ=255)

                                          aCCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCa                                                                                                            <  1:2537434/150‑1 (MQ=255)

                                          aCCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCa                                                                                                            >  2:3767734/1‑150 (MQ=255)

                                           cccGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAg                                                                                                           >  2:3474046/1‑150 (MQ=255)

                                            ccGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGc                                                                                                          >  1:681354/1‑150 (MQ=255)

                                            ccGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCGGCCAGc                                                                                                          >  1:4155555/1‑150 (MQ=255)

                                                  cTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGa                                                                                                    <  1:1540170/150‑1 (MQ=255)

                                                   tATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGAc                                                                                                   >  2:1533293/1‑150 (MQ=255)

                                                     tGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGt                                                                                                 >  1:3612159/1‑150 (MQ=255)

                                                      ggCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTg                                                                                                <  1:2131284/150‑1 (MQ=255)

                                                         tttGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGcc                                                                                             >  1:2206969/1‑150 (MQ=255)

                                                            gTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGc                                                                                          <  1:67760/150‑1 (MQ=255)

                                                              cTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCACGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCa                                                                                        <  2:148348/150‑1 (MQ=255)

                                                              cTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGccctggc                                                                                        <  1:1641381/150‑5 (MQ=255)

                                                              cTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCa                                                                                        <  2:288001/150‑1 (MQ=255)

                                                              cTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCa                                                                                        <  2:1758382/150‑1 (MQ=255)

                                                              cTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCa                                                                                        >  1:1849991/1‑150 (MQ=255)

                                                               tGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAg                                                                                       >  2:1932175/1‑150 (MQ=255)

                                                               tGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAg                                                                                       >  1:261752/1‑150 (MQ=255)

                                                               tGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAg                                                                                       >  2:4476264/1‑150 (MQ=255)

                                                                gggAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAgg                                                                                      >  2:1431564/1‑150 (MQ=255)

                                                                 ggAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGc                                                                                     >  2:2615090/1‑150 (MQ=255)

                                                                  gAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGcc                                                                                    >  2:3278721/1‑150 (MQ=255)

                                                                       caccTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGc                                                                               >  1:1418093/1‑150 (MQ=255)

                                                                       caccTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGc                                                                               <  2:283021/150‑1 (MQ=255)

                                                                         ccgtGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACCGCCGGCCCGCCCGGGACGTGGCCCGCCCGGCCGGGGCCa                                                                             >  1:542808/4‑150 (MQ=255)

                                                                            tGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgct                                                                          <  2:1971798/150‑1 (MQ=255)

                                                                                cgccgcCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAgctggct                                                                      >  2:2462677/1‑150 (MQ=255)

                                                                                    gccgACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTc                                                                  >  2:338932/1‑150 (MQ=255)

                                                                                       gACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGc                                                               >  1:174620/1‑150 (MQ=255)

                                                                                       gACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGAAGAAGACAATATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCAGGCTGCTCGGc                                                               >  1:871588/1‑150 (MQ=255)

                                                                                       gACCCGTTCATTCACCTGACCCAAGCACGGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGc                                                               <  2:3650706/150‑1 (MQ=255)

                                                                                          ccGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTTGGCTTAGCCGTGACCGACGAAGCCATGATCCGCGCCAGCGGCGACACCCTGCCACCCCAGGACGTGGCCCCCCAGGCCAGGGCCAGCTGGCTGCTCGGCgtg                                                            >  1:410602/1‑150 (MQ=255)

                                                                                          ccGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtg                                                            >  1:3500437/1‑150 (MQ=255)

                                                                                          ccGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtg                                                            >  2:3926568/1‑150 (MQ=255)

                                                                                            gTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATTATACGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCgtggt                                                          >  1:800727/1‑150 (MQ=255)

                                                                                              tcattcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGc                                                        <  1:1165/150‑1 (MQ=255)

                                                                                              tcattcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGc                                                        <  1:4415406/150‑1 (MQ=255)

                                                                                              tcattcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGGTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGc                                                        <  2:3116088/150‑1 (MQ=255)

                                                                                                attcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTg                                                      >  1:1363490/1‑150 (MQ=255)

                                                                                                attcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTg                                                      >  2:2694586/1‑150 (MQ=255)

                                                                                                 ttcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTgg                                                     >  1:2452802/1‑150 (MQ=255)

                                                                                                  tcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGt                                                    <  1:1069803/150‑1 (MQ=255)

                                                                                                  tcaCCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGt                                                    <  2:3368792/150‑1 (MQ=255)

                                                                                                       tGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACg                                               >  1:1283319/1‑150 (MQ=255)

                                                                                                           ccAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCct                                           >  2:119673/1‑150 (MQ=255)

                                                                                                           ccAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCct                                           >  2:4394976/1‑150 (MQ=255)

                                                                                                           ccAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCGGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCct                                           >  1:2259676/1‑150 (MQ=255)

                                                                                                            cAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCctg                                          >  2:3519913/1‑150 (MQ=255)

                                                                                                            cAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCAGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCctg                                          >  2:4316403/1‑150 (MQ=255)

                                                                                                                 aCTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGcc                                     >  1:1479213/1‑150 (MQ=255)

                                                                                                                   tGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGcccc                                   <  2:3109303/150‑1 (MQ=255)

                                                                                                                      gcgcCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGcc                                >  2:2831781/1‑150 (MQ=255)

                                                                                                                        gcCCTCCCGTCGTTGCTGGGCTTCGCCCTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctg                              >  1:1309904/1‑150 (MQ=255)

                                                                                                                          ccTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCCGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgct                            <  1:1786237/150‑1 (MQ=255)

                                                                                                                          ccTCCCGCCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgct                            <  1:3820940/150‑1 (MQ=255)

                                                                                                                           cTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctg                           >  2:2238052/1‑150 (MQ=255)

                                                                                                                           cTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctg                           <  1:2155412/150‑1 (MQ=255)

                                                                                                                           cTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctg                           <  1:4236107/150‑1 (MQ=255)

                                                                                                                            tCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctgc                          >  2:739888/1‑150 (MQ=255)

                                                                                                                              ccGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCctgctgctg                        <  2:1803602/150‑1 (MQ=255)

                                                                                                                                  cgtTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCg                    <  2:584088/150‑1 (MQ=255)

                                                                                                                                   gtTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCgg                   <  1:4363912/150‑1 (MQ=255)

                                                                                                                                   gtTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCgg                   >  1:2715766/1‑150 (MQ=255)

                                                                                                                                   gtTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCgg                   <  2:283766/150‑1 (MQ=255)

                                                                                                                                        tgtgCTTCGCCGTGCCCGACGAAGCCATTATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGACATGCCTGTGCCAGCTGGCTGCTCGGCGTGTTGCTTGTATACTGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTAt              <  1:3356539/147‑1 (MQ=255)

                                                                                                                                         tggCTTCGTCGGGCACGGCGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATg             >  2:1352633/2‑150 (MQ=255)

                                                                                                                                         gggCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATg             >  1:953512/1‑150 (MQ=255)

                                                                                                                                         gggCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATg             >  1:3663968/1‑150 (MQ=255)

                                                                                                                                         gggCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATg             >  1:464830/1‑150 (MQ=255)

                                                                                                                                          ggCTTCGTCGGGCACGGCGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGc            >  2:2636821/1‑150 (MQ=255)

                                                                                                                                           gCTTCGTCGGGCACGGCGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGcc           >  1:3864111/1‑150 (MQ=255)

                                                                                                                                            cTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCt          >  2:410785/1‑150 (MQ=255)

                                                                                                                                             ttCGTCGGGCACGGCGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGCCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTCTGCCtg         >  2:4518186/1‑150 (MQ=255)

                                                                                                                                             ttCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGccct         <  2:3885394/150‑3 (MQ=255)

                                                                                                                                             ttCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGccct         <  2:1136312/150‑3 (MQ=255)

                                                                                                                                             ttCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCtg         >  1:3736345/1‑150 (MQ=255)

                                                                                                                                                 ccGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCTGtggc     <  1:3133742/150‑1 (MQ=255)

                                                                                                                                                   gTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCTGtggcgc   >  2:3613275/1‑150 (MQ=255)

GCGATGCTCACCGCGCTGGGCATGCTACTGGTCTTGCTGGCGACCCGGCGCTATGGCTTTGTCTGGGAAACCACCTTGCTCGCCGCCGACCCGTTCATTCACCTGACCCAAGCACTGGGCGCCCTCCCGTCGTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGCCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCT  >  NC_002947/5858610‑5858907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`