BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,970,415	A→C	5.9%	L45L (CTT→CTG) ‡	PP_5723 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,970,415	0	A	C	5.9%	310.8 / 7.2	100	L45L (CTT→CTG) ‡	PP_5723	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (35/57); new base C (4/2); total (41/59)
Fisher's exact test for biased strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

ATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGTG  >  NC_002947/5970267‑5970564

aTTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCGACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGaa                                                                                                                                                       <  2:2278784/150‑1 (MQ=255)

 tttGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAg                                                                                                                                                      <  1:1312272/150‑1 (MQ=255)

   gcatCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGGGTGGCGGTTGGGGGGGGGAgcg                                                                                                                                                    >  2:1830949/4‑150 (MQ=255)

    gCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAgcgc                                                                                                                                                   <  1:481101/150‑1 (MQ=255)

     cTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGcc                                                                                                                                                  <  1:870605/150‑1 (MQ=255)

      tCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGccc                                                                                                                                                 >  2:501291/1‑150 (MQ=255)

       cGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGcccc                                                                                                                                                <  1:4056275/150‑1 (MQ=255)

          gATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCa                                                                                                                                             <  1:803600/150‑1 (MQ=255)

           aTTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGccccaatc                                                                                                                                            <  1:4353419/150‑5 (MQ=255)

           aTTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCaa                                                                                                                                            <  1:1935296/150‑1 (MQ=255)

           aTTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCaa                                                                                                                                            <  1:3411418/150‑1 (MQ=255)

           aTTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCaa                                                                                                                                            <  1:3411442/150‑1 (MQ=255)

             tGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGa                                                                                                                                          <  2:3793665/150‑1 (MQ=255)

             tGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCGTGAAAACTGCACGCGATCGTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGa                                                                                                                                          <  2:1686204/150‑1 (MQ=255)

              ggCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAt                                                                                                                                         <  2:1178224/150‑1 (MQ=255)

              ggCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAt                                                                                                                                         <  2:1178182/150‑1 (MQ=255)

                ccAGTCGCCCGCACGGGAAACCTGCACACTTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAttt                                                                                                                                       <  1:2591045/150‑1 (MQ=255)

                ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAttt                                                                                                                                       <  2:4286466/150‑1 (MQ=255)

                ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCGGGGTTTGGGTGGGATGGGCCCCCCCAGGttt                                                                                                                                       >  1:4105499/1‑150 (MQ=255)

                  aGTCGCCAGCCCGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCACGCTGCGCGAAAAATGGGCAACAGCTTGAAAACGGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                                     <  2:2346173/150‑1 (MQ=255)

                  aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                                     <  2:3607952/150‑1 (MQ=255)

                  aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                                     <  2:1068511/150‑1 (MQ=255)

                  aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                                     <  2:3698848/150‑1 (MQ=255)

                  aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                                     <  1:220592/150‑1 (MQ=255)

                  aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGACTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                                     <  1:331987/150‑1 (MQ=255)

                    tCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGc                                                                                                                                   <  1:1930780/150‑1 (MQ=255)

                      gCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCt                                                                                                                                 <  1:2664550/150‑1 (MQ=255)

                       ccAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCtt                                                                                                                                <  2:1402872/150‑1 (MQ=255)

                       ccAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCtt                                                                                                                                <  2:1886651/150‑1 (MQ=255)

                       ccAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCtt                                                                                                                                <  1:960202/150‑1 (MQ=255)

                        cAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTg                                                                                                                               <  1:45622/150‑1 (MQ=255)

                               ggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGa                                                                                                                        <  1:3748552/150‑1 (MQ=255)

                                 aaaCATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGat‑a                                                                                                                     <  1:4071094/150‑1 (MQ=255)

                                   aCATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑Att                                                                                                                   <  1:206149/150‑1 (MQ=255)

                                     aTGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCCGCGCGCTTGGGGTGCGAAGCCCCCCCCCACCTTTGTGCCGTGTTGCTGTG‑ATTgg                                                                                                                 >  2:2941872/1‑149 (MQ=255)

                                      tGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGcc                                                                                                                <  1:822788/150‑1 (MQ=255)

                                        cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCgg                                                                                                              <  2:1209592/150‑1 (MQ=255)

                                        cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCgg                                                                                                              <  2:3851859/150‑1 (MQ=255)

                                        cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCgg                                                                                                              <  2:3851901/150‑1 (MQ=255)

                                        cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCgg                                                                                                              <  2:3146637/150‑1 (MQ=255)

                                        cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCgg                                                                                                              <  1:1210079/150‑1 (MQ=255)

                                         acaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCggg                                                                                                             <  1:536592/150‑1 (MQ=255)

                                               tCGGTGCTCAGCTGCTGGGCATTATTCGCGTAAAATGTGCTAAAGCTTGAAATCTGCTGGATATCTTTGTTGGAGGGGCCTTTCGTCGCGATTGGGTTTCGAAGCGGCCCCCTTGATTTGTTGCTTGATGCTGAT‑ATTGCCGTGTCCGCt                                                                                                       <  1:1210856/150‑1 (MQ=255)

                                                    gcgcAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCa                                                                                                  <  1:1862274/150‑1 (MQ=255)

                                                     cgcAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAg                                                                                                 <  1:501291/150‑1 (MQ=255)

                                                      gcagcaGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGc                                                                                                <  2:2069609/150‑1 (MQ=255)

                                                        agcagcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGGTTGGGGTGGGAAGCGCCCCCCAAGATTTGTGGGGTGTTTGTGGT‑ATTTGCGGGGGCGCGGTGCAGgcc                                                                                              >  2:3709076/1‑150 (MQ=255)

                                                            gcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATc                                                                                          <  1:2514035/150‑1 (MQ=255)

                                                            gcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATc                                                                                          <  1:1244889/150‑1 (MQ=255)

                                                            gcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATc                                                                                          <  2:1312872/150‑1 (MQ=255)

                                                              ggggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATcgc                                                                                        <  2:559063/150‑1 (MQ=255)

                                                                 gCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGAc                                                                                     <  1:1965385/150‑1 (MQ=255)

                                                                   aaGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGc                                                                                   <  2:1593232/150‑1 (MQ=255)

                                                                      cTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAg                                                                                <  2:1517274/150‑1 (MQ=255)

                                                                          gcgAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCGTGCGGCGCGAAGGGGGTTCCAAGCGACCCCCCAGAATTGTGGCCTGTTGGTGAT‑AATGGCCGGGCCCCCGGGCCGCCCCAACCCGCCGCAAGGCCg                                                                            >  2:1898748/1‑150 (MQ=255)

                                                                            gAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGGGCGCATGGGGTGGGCATCCCCCCCCCACCATTTTGCCCGGGTGCGGGTAATTGAC‑GGGCCGCAGGGCAGCCCCAACGCCACGCAACGccccc                                                                          >  2:2865736/1‑147 (MQ=255)

                                                                            gAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGGGTGGGGGGGGAAGCGCCCCCCAACAATTGTTGCTTGTAGCTGAT‑AATTGCGGGGCCCCGGGTGAGCCCAATAGCCACGCCAAGCCCca                                                                          >  2:1818164/1‑150 (MQ=255)

                                                                                                 aaaCTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGCGGCCTTGGGGTGGGGAACCCCCCCCAACAATTTTGGCGTGATGGTGGT‑GTTTGCGGGGCCGCGGTGCCGCCCAAACGCCAAGCCAGGCCCCACACAAAAGCCCAggtggaaga                                                     >  2:2707450/1‑145 (MQ=255)

                                                                                                        ccgctttCTTTGTGGGCGGGGCTTTGGGTGGGTTTGGGGGTGCGACCCCCCCCCCAATTGTGGGGCCTGTATGCTTAT‑TTGGCGGGGGCCGGTGTGCCCCCCATCCGCCCCCCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGcc                                              <  2:3595484/144‑1 (MQ=255)

                                                                                                         cgcgCTGTTGGTGGGCGCGGCCTGGGGGCGCGTTGGGGGGGCGACGCCCCCCCCAAGTTTGGTGCCGTGATGCTGAT‑ATCGCGGGGGCCGCGGTGCCCCCCACCCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCa                                             <  2:4105499/150‑1 (MQ=255)

                                                                                                          gcgATCTTTGTGGGAGCGGCCTTGCGGCGCGTTTGGGGTGCGAACCGCCCCCCCAGATTTGTTGCGGGGTGCTGGT‑AATGCCGGGGCCCCAGGGGAGCCCAAAGGCGACGCAAGGCCCCCCACCAAGGCCAATGGGGACAAAGGCGcccg                                            >  2:313241/1‑148 (MQ=255)

                                                                                                                ttggtgGGCGCGGCCTGGCGTGGCGTTGGGGGTGCGATGCCCCCCCCATTTTTGGTGCTGTGTGGCTTTT‑TTGGCGGGGGCGGCTGGGCCGCCCAATCGCGCCGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTtgt                                      <  2:3333926/147‑1 (MQ=255)

                                                                                                                  tgtgGGTGCGGCCTTGCTTCGCTTTTGGGGCGCGACGCCCCCCCCATGTTTTGTGCGTTGTGGCTGTT‑TTGGCCGGGGCCGGTGTGCCGCCCACTCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTgtgc                                    <  2:4467382/150‑1 (MQ=255)

                                                                                                                        aGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGcc                                                                                               >  2:2453104/1‑85 (MQ=255)

                                                                                                                        aGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGcc                                                                                               <  1:2453104/85‑1 (MQ=255)

                                                                                                                             ccTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTg                         >  1:1779021/1‑150 (MQ=255)

                                                                                                                              cTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTgg                        >  1:287916/1‑150 (MQ=255)

                                                                                                                               ttGCGTCGCTTTTGGGGTGCGACGCGCCCCAAAAGATTTGTGCATTTTTGGTTTT‑TTTGCGGGGGCGGGTGTGCAGCACAATCGCGCCGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                       <  2:2575931/150‑1 (MQ=255)

                                                                                                                               ttGCGTCGCAATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                       >  1:148076/1‑150 (MQ=255)

                                                                                                                                  cGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggcggc                    >  2:3053194/1‑150 (MQ=255)

                                                                                                                                   gTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                   >  2:4425912/1‑150 (MQ=255)

                                                                                                                                   gTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                   >  2:631565/1‑150 (MQ=255)

                                                                                                                                   gTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                   >  1:929827/1‑150 (MQ=255)

                                                                                                                                   gTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                   >  2:4426096/1‑150 (MQ=255)

                                                                                                                                   acaaatcttGGGGGGCGCTTCGCACCCCAATATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                   >  1:2499420/8‑150 (MQ=255)

                                                                                                                                    tCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCCGGTTTGTGCGGGCGACACTGGCGGCCa                  >  1:1575453/1‑150 (MQ=255)

                                                                                                                                    tCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCa                  >  1:3466522/1‑150 (MQ=255)

                                                                                                                                    tCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCa                  >  1:625315/1‑150 (MQ=255)

                                                                                                                                       atcttGGGGGGCGCTTCGCACCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                       >  2:679836/4‑142 (MQ=255)

                                                                                                                                       atcttGGGGGGCGCTTCGCACCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                       <  1:679907/139‑1 (MQ=255)

                                                                                                                                       atcttGGGGGGCGCTTCGCACCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                       >  2:679907/4‑142 (MQ=255)

                                                                                                                                       atcttGGGGGGCGCTTCGCACCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                       <  1:679836/139‑1 (MQ=255)

                                                                                                                                        gATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTaca                            >  1:1148636/1‑136 (MQ=255)

                                                                                                                                        gATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTtc              >  2:613138/1‑150 (MQ=255)

                                                                                                                                             gggTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGt         >  2:3551430/1‑150 (MQ=255)

                                                                                                                                              ggTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGtt        >  1:2794808/1‑150 (MQ=255)

                                                                                                                                              ggTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGtt        >  1:2613434/1‑150 (MQ=255)

                                                                                                                                               gTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGttt       >  2:1592061/1‑150 (MQ=255)

                                                                                                                                               gTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGttt       >  2:1470703/1‑150 (MQ=255)

                                                                                                                                               gTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGttt       >  2:1435162/1‑150 (MQ=255)

                                                                                                                                               gTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGttt       >  1:3609113/1‑150 (MQ=255)

                                                                                                                                                tGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAgg                                                                  >  2:452558/1‑90 (MQ=255)

                                                                                                                                                tGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAgg                                                                  <  1:452558/90‑1 (MQ=255)

                                                                                                                                                tGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTc      >  2:3989412/1‑150 (MQ=255)

                                                                                                                                                 gCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTACCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCa     >  1:3096666/1‑150 (MQ=255)

                                                                                                                                                   gAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGt   >  1:2986984/1‑150 (MQ=255)

                                                                                                                                                   gAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGt   >  2:2849274/1‑150 (MQ=255)

                                                                                                                                                   gAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGt   >  1:86954/1‑150 (MQ=255)

                                                                                                                                                   gAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTGTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGt   >  2:861569/1‑150 (MQ=255)

                                                                                                                                                    aaGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGTg  >  1:2348277/1‑150 (MQ=255)

ATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGTG  >  NC_002947/5970267‑5970564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`