BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,970,421	C→A	5.2%	G43G (GGG→GGT)	PP_5723 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,970,421	0	C	A	5.2%	327.4 / 6.4	96	G43G (GGG→GGT)	PP_5723	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (38/53); new base A (3/2); total (41/55)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 103 total aligned reads displayed.

CTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGTGGACAG  >  NC_002947/5970272‑5970569

cTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGcc                                                                                                                                                       <  1:870605/150‑1 (MQ=255)

 tCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGccc                                                                                                                                                      >  2:501291/1‑150 (MQ=255)

  cGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGcccc                                                                                                                                                     <  1:4056275/150‑1 (MQ=255)

     gATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCa                                                                                                                                                  <  1:803600/150‑1 (MQ=255)

      aTTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCaa                                                                                                                                                 <  1:3411442/150‑1 (MQ=255)

      aTTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCaa                                                                                                                                                 <  1:3411418/150‑1 (MQ=255)

      aTTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCaa                                                                                                                                                 <  1:1935296/150‑1 (MQ=255)

        tGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGa                                                                                                                                               <  2:3793665/150‑1 (MQ=255)

        tGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCGTGAAAACTGCACGCGATCGTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGa                                                                                                                                               <  2:1686204/150‑1 (MQ=255)

         ggCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAt                                                                                                                                              <  2:1178182/150‑1 (MQ=255)

         ggCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAt                                                                                                                                              <  2:1178224/150‑1 (MQ=255)

           ccAGTCGCCCGCACGGGAAACCTGCACACTTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAttt                                                                                                                                            <  1:2591045/150‑1 (MQ=255)

           ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAttt                                                                                                                                            <  2:4286466/150‑1 (MQ=255)

           ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCGGGGTTTGGGTGGGATGGGCCCCCCCAGGttt                                                                                                                                            >  1:4105499/1‑150 (MQ=255)

             aGTCGCCAGCCCGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCACGCTGCGCGAAAAATGGGCAACAGCTTGAAAACGGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                                          <  2:2346173/150‑1 (MQ=255)

             aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                                          <  1:220592/150‑1 (MQ=255)

             aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                                          <  2:3698848/150‑1 (MQ=255)

             aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                                          <  2:3607952/150‑1 (MQ=255)

             aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                                          <  2:1068511/150‑1 (MQ=255)

             aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGACTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                                          <  1:331987/150‑1 (MQ=255)

               tCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGc                                                                                                                                        <  1:1930780/150‑1 (MQ=255)

                 gCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCt                                                                                                                                      <  1:2664550/150‑1 (MQ=255)

                  ccAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCtt                                                                                                                                     <  1:960202/150‑1 (MQ=255)

                  ccAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCtt                                                                                                                                     <  2:1402872/150‑1 (MQ=255)

                  ccAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCtt                                                                                                                                     <  2:1886651/150‑1 (MQ=255)

                   cAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTg                                                                                                                                    <  1:45622/150‑1 (MQ=255)

                          ggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGa                                                                                                                             <  1:3748552/150‑1 (MQ=255)

                            aaaCATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGat‑a                                                                                                                          <  1:4071094/150‑1 (MQ=255)

                              aCATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑Att                                                                                                                        <  1:206149/150‑1 (MQ=255)

                                aTGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCCGCGCGCTTGGGGTGCGAAGCCCCCCCCCACCTTTGTGCCGTGTTGCTGTG‑ATTgg                                                                                                                      >  2:2941872/1‑149 (MQ=255)

                                 tGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGcc                                                                                                                     <  1:822788/150‑1 (MQ=255)

                                   cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCgg                                                                                                                   <  2:3146637/150‑1 (MQ=255)

                                   cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCgg                                                                                                                   <  2:1209592/150‑1 (MQ=255)

                                   cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCgg                                                                                                                   <  1:1210079/150‑1 (MQ=255)

                                   cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCgg                                                                                                                   <  2:3851859/150‑1 (MQ=255)

                                   cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCgg                                                                                                                   <  2:3851901/150‑1 (MQ=255)

                                    acaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCggg                                                                                                                  <  1:536592/150‑1 (MQ=255)

                                          tCGGTGCTCAGCTGCTGGGCATTATTCGCGTAAAATGTGCTAAAGCTTGAAATCTGCTGGATATCTTTGTTGGAGGGGCCTTTCGTCGCGATTGGGTTTCGAAGCGGCCCCCTTGATTTGTTGCTTGATGCTGAT‑ATTGCCGTGTCCGCt                                                                                                            <  1:1210856/150‑1 (MQ=255)

                                               gcgcAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCa                                                                                                       <  1:1862274/150‑1 (MQ=255)

                                                cgcAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAg                                                                                                      <  1:501291/150‑1 (MQ=255)

                                                 gcagcaGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGc                                                                                                     <  2:2069609/150‑1 (MQ=255)

                                                   agcagcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGGTTGGGGTGGGAAGCGCCCCCCAAGATTTGTGGGGTGTTTGTGGT‑ATTTGCGGGGGCGCGGTGCAGgcc                                                                                                   >  2:3709076/1‑150 (MQ=255)

                                                       gcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATc                                                                                               <  1:1244889/150‑1 (MQ=255)

                                                       gcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATc                                                                                               <  1:2514035/150‑1 (MQ=255)

                                                       gcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATc                                                                                               <  2:1312872/150‑1 (MQ=255)

                                                         ggggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATcgc                                                                                             <  2:559063/150‑1 (MQ=255)

                                                            gCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGAc                                                                                          <  1:1965385/150‑1 (MQ=255)

                                                              aaGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGc                                                                                        <  2:1593232/150‑1 (MQ=255)

                                                                 cTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAg                                                                                     <  2:1517274/150‑1 (MQ=255)

                                                                     gcgAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCGTGCGGCGCGAAGGGGGTTCCAAGCGACCCCCCAGAATTGTGGCCTGTTGGTGAT‑AATGGCCGGGCCCCCGGGCCGCCCCAACCCGCCGCAAGGCCg                                                                                 >  2:1898748/1‑150 (MQ=255)

                                                                       gAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGGGCGCATGGGGTGGGCATCCCCCCCCCACCATTTTGCCCGGGTGCGGGTAATTGAC‑GGGCCGCAGGGCAGCCCCAACGCCACGCAACGccccc                                                                               >  2:2865736/1‑147 (MQ=255)

                                                                       gAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGGGTGGGGGGGGAAGCGCCCCCCAACAATTGTTGCTTGTAGCTGAT‑AATTGCGGGGCCCCGGGTGAGCCCAATAGCCACGCCAAGCCCca                                                                               >  2:1818164/1‑150 (MQ=255)

                                                                                            aaaCTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGCGGCCTTGGGGTGGGGAACCCCCCCCAACAATTTTGGCGTGATGGTGGT‑GTTTGCGGGGCCGCGGTGCCGCCCAAACGCCAAGCCAGGCCCCACACAAAAGCCCAggtggaaga                                                          >  2:2707450/1‑145 (MQ=255)

                                                                                                   ccgctttCTTTGTGGGCGGGGCTTTGGGTGGGTTTGGGGGTGCGACCCCCCCCCCAATTGTGGGGCCTGTATGCTTAT‑TTGGCGGGGGCCGGTGTGCCCCCCATCCGCCCCCCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGcc                                                   <  2:3595484/144‑1 (MQ=255)

                                                                                                    cgcgCTGTTGGTGGGCGCGGCCTGGGGGCGCGTTGGGGGGGCGACGCCCCCCCCAAGTTTGGTGCCGTGATGCTGAT‑ATCGCGGGGGCCGCGGTGCCCCCCACCCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCa                                                  <  2:4105499/150‑1 (MQ=255)

                                                                                                     gcgATCTTTGTGGGAGCGGCCTTGCGGCGCGTTTGGGGTGCGAACCGCCCCCCCAGATTTGTTGCGGGGTGCTGGT‑AATGCCGGGGCCCCAGGGGAGCCCAAAGGCGACGCAAGGCCCCCCACCAAGGCCAATGGGGACAAAGGCGcccg                                                 >  2:313241/1‑148 (MQ=255)

                                                                                                           ttggtgGGCGCGGCCTGGCGTGGCGTTGGGGGTGCGATGCCCCCCCCATTTTTGGTGCTGTGTGGCTTTT‑TTGGCGGGGGCGGCTGGGCCGCCCAATCGCGCCGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTtgt                                           <  2:3333926/147‑1 (MQ=255)

                                                                                                             tgtgGGTGCGGCCTTGCTTCGCTTTTGGGGCGCGACGCCCCCCCCATGTTTTGTGCGTTGTGGCTGTT‑TTGGCCGGGGCCGGTGTGCCGCCCACTCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTgtgc                                         <  2:4467382/150‑1 (MQ=255)

                                                                                                                   aGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGcc                                                                                                    <  1:2453104/85‑1 (MQ=255)

                                                                                                                        ccTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTg                              >  1:1779021/1‑150 (MQ=255)

                                                                                                                         cTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTgg                             >  1:287916/1‑150 (MQ=255)

                                                                                                                          ttGCGTCGCTTTTGGGGTGCGACGCGCCCCAAAAGATTTGTGCATTTTTGGTTTT‑TTTGCGGGGGCGGGTGTGCAGCACAATCGCGCCGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                            <  2:2575931/150‑1 (MQ=255)

                                                                                                                          ttGCGTCGCAATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                            >  1:148076/1‑150 (MQ=255)

                                                                                                                             cGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggcggc                         >  2:3053194/1‑150 (MQ=255)

                                                                                                                              gTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                        >  2:4426096/1‑150 (MQ=255)

                                                                                                                              gTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                        >  2:4425912/1‑150 (MQ=255)

                                                                                                                              gTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                        >  2:631565/1‑150 (MQ=255)

                                                                                                                              gTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                        >  1:929827/1‑150 (MQ=255)

                                                                                                                              acaaatcttGGGGGGCGCTTCGCACCCCAATATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                        >  1:2499420/8‑150 (MQ=255)

                                                                                                                               tCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCCGGTTTGTGCGGGCGACACTGGCGGCCa                       >  1:1575453/1‑150 (MQ=255)

                                                                                                                               tCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCa                       >  1:3466522/1‑150 (MQ=255)

                                                                                                                               tCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCa                       >  1:625315/1‑150 (MQ=255)

                                                                                                                                  atcttGGGGGGCGCTTCGCACCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                            >  2:679836/4‑142 (MQ=255)

                                                                                                                                  atcttGGGGGGCGCTTCGCACCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                            >  2:679907/4‑142 (MQ=255)

                                                                                                                                  atcttGGGGGGCGCTTCGCACCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                            <  1:679836/139‑1 (MQ=255)

                                                                                                                                  atcttGGGGGGCGCTTCGCACCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                            <  1:679907/139‑1 (MQ=255)

                                                                                                                                   gATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTaca                                 >  1:1148636/1‑136 (MQ=255)

                                                                                                                                   gATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTaca                                 <  2:1148636/136‑1 (MQ=255)

                                                                                                                                   gATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTtc                   >  2:613138/1‑150 (MQ=255)

                                                                                                                                        gggTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGt              >  2:3551430/1‑150 (MQ=255)

                                                                                                                                         ggTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGtt             >  1:2794808/1‑150 (MQ=255)

                                                                                                                                         ggTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGtt             >  1:2613434/1‑150 (MQ=255)

                                                                                                                                          gTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGttt            >  2:1592061/1‑150 (MQ=255)

                                                                                                                                          gTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGttt            >  1:3609113/1‑150 (MQ=255)

                                                                                                                                          gTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGttt            >  2:1435162/1‑150 (MQ=255)

                                                                                                                                          gTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGttt            >  2:1470703/1‑150 (MQ=255)

                                                                                                                                           tGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAgg                                                                       <  1:452558/90‑1 (MQ=255)

                                                                                                                                           tGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAgg                                                                       >  2:452558/1‑90 (MQ=255)

                                                                                                                                           tGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTc           >  2:3989412/1‑150 (MQ=255)

                                                                                                                                            gCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTACCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCa          >  1:3096666/1‑150 (MQ=255)

                                                                                                                                             cccaGCGCCCCCCATGTTTGGTGCTTTGCTGCTGAT‑TTGGCGGGGGCCGCTGGGCCCCCCACCCGCGCCGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAg         <  2:2191581/147‑1 (MQ=255)

                                                                                                                                              gAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGt        >  1:2986984/1‑150 (MQ=255)

                                                                                                                                              gAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGt        >  2:2849274/1‑150 (MQ=255)

                                                                                                                                              gAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGt        >  1:86954/1‑150 (MQ=255)

                                                                                                                                              gAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTGTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGt        >  2:861569/1‑150 (MQ=255)

                                                                                                                                               aaGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGTg       >  1:2348277/1‑150 (MQ=255)

                                                                                                                                                 gccccccccAAGTTTGGTGCCTTGCTGCTGTT‑TTTGCGGGGGCCGGTGTGCCGCCCACCCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGTGGa     <  2:1832802/147‑1 (MQ=255)

                                                                                                                                                   gcCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGTGGACa   >  1:1381956/1‑150 (MQ=255)

                                                                                                                                                    ccccccAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGTGGACAg  >  2:2983198/1‑150 (MQ=255)

                                                                                                                                                    ccccccAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGTGGACAg  >  2:263884/1‑150 (MQ=255)

CTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGAT‑ATTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGTGGACAG  >  NC_002947/5970272‑5970569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`