BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	450,924	G→A	7.0%	noncoding (118/127 nt)	PP_mr05 →	RsmY ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	450,924	0	G	A	7.0%	627.0 / 24.0	200	noncoding (118/127 nt)	PP_mr05	RsmY ncRNA
Reads supporting (aligned to +/- strand): ref base G (68/118); new base A (3/11); total (71/129)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

TCTAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCAGGCCGA  >  NC_002947/450775‑451073

  tAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTcggc                                                                                                                                                     >  2:3023302/1‑150 (MQ=255)

  tAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTcggc                                                                                                                                                     >  2:599946/1‑150 (MQ=255)

  tAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTcggc                                                                                                                                                     >  2:3564140/1‑150 (MQ=255)

   aaGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTcggcg                                                                                                                                                    >  2:1513693/1‑150 (MQ=255)

   aaGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTcggcg                                                                                                                                                    <  2:2856129/150‑1 (MQ=255)

   aaGATAGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTcggcg                                                                                                                                                    >  2:4133245/1‑150 (MQ=255)

    aGATTGGATCCCCTGTTACTCTTTACCACATGGACGGTGCGCAGGAAGCGCAAAGGACAGAGCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGAGGTCACAGGGAAACAGTCTGGAAAACCCCGCGTcggcgg                                                                                                                                                   <  2:2541288/150‑1 (MQ=255)

    aGATTGGATCCACTGTGAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTcggcgg                                                                                                                                                   <  2:3036895/150‑1 (MQ=255)

     gATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCggg                                                                                                                                                  <  2:1544472/150‑1 (MQ=255)

         ggATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCCGAAGCGGGGttt                                                                                                                                              <  1:1879205/150‑1 (MQ=255)

         ggATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCCGAAGCGGGGttt                                                                                                                                              <  2:2727009/150‑1 (MQ=255)

         ggATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCCGAAGCGGGGttt                                                                                                                                              <  2:2080252/150‑1 (MQ=255)

         ggATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTAGAAAACCCCGCCGAAGCGGGGttt                                                                                                                                              <  1:3062848/150‑1 (MQ=255)

         ggATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTAGAAAACCCCGCCGAAGCGGGGttt                                                                                                                                              <  1:793366/150‑1 (MQ=255)

           aTCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCCGAAGCGGGGTTTTc                                                                                                                                            <  2:174625/150‑1 (MQ=255)

            tCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTAGAAAACCCCGCCGAAGCGGGGTTTTcc                                                                                                                                           <  2:2399667/150‑2 (MQ=255)

               aCTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTtg                                                                                                                                        <  1:1040228/150‑1 (MQ=255)

               aCTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTAGAAAACCCCGCCGAAGCGGGGTTTTccaga                                                                                                                                        <  2:4063268/150‑5 (MQ=255)

                 tGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTtgcg                                                                                                                                      >  2:1239064/1‑150 (MQ=255)

                 tGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTtgcg                                                                                                                                      <  2:3691168/150‑1 (MQ=255)

                  gtgaaTCCTTACCACAGGGACGTCGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGACACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTtgcgt                                                                                                                                     <  2:829137/147‑1 (MQ=255)

                   ttAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAATCCCCGCTTCGGCGGGGTTTTCTTtgcgtg                                                                                                                                    >  2:3028383/1‑150 (MQ=255)

                    tAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTtgcgtgc                                                                                                                                   <  1:2022657/150‑1 (MQ=255)

                      aTCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTgcgtgcgt                                                                                                                                 <  1:446373/150‑1 (MQ=255)

                             ccACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTc                                                                                                                          <  2:2882274/150‑1 (MQ=255)

                              cacaTGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCa                                                                                                                         <  1:3152109/150‑1 (MQ=255)

                              cacaTGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCa                                                                                                                         <  2:1868473/150‑1 (MQ=255)

                               acaTGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAg                                                                                                                        <  2:1460574/150‑1 (MQ=255)

                                   ggACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCa                                                                                                                    <  1:3723595/150‑1 (MQ=255)

                                    gACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAg                                                                                                                   <  1:522717/150‑1 (MQ=255)

                                    gACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAg                                                                                                                   <  2:4391815/150‑1 (MQ=255)

                                          gcgcAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATc                                                                                                             <  1:977785/150‑1 (MQ=255)

                                            gcAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCa                                                                                                           >  1:2052579/1‑150 (MQ=255)

                                            gcAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCa                                                                                                           >  1:3417348/1‑150 (MQ=255)

                                                 aaGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGt                                                                                                      <  1:1766640/150‑1 (MQ=255)

                                                   gcgcAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGa                                                                                                    <  2:3959539/150‑1 (MQ=255)

                                                    cgcAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGTGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGAt                                                                                                   <  2:1995509/150‑1 (MQ=255)

                                                     gcAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATg                                                                                                  <  2:2870902/150‑1 (MQ=255)

                                                              aGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGTCAGCACATCCAGCGGTGATGCTCCCATCa                                                                                         <  1:4075161/150‑1 (MQ=255)

                                                                   aaGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGa                                                                                    <  1:3194733/150‑1 (MQ=255)

                                                                   aaGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGa                                                                                    <  1:4170062/150‑1 (MQ=255)

                                                                    aGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAg                                                                                   >  2:114127/1‑150 (MQ=255)

                                                                         gacgacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtcc                                                                              >  1:3960770/1‑150 (MQ=255)

                                                                            gacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtccgtc                                                                           <  1:65000/150‑1 (MQ=255)

                                                                            gacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtccgtc                                                                           >  1:2228138/1‑150 (MQ=255)

                                                                            gacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtccgtc                                                                           <  1:3517892/150‑1 (MQ=255)

                                                                              cgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGtctc                                                                         <  2:2254644/150‑1 (MQ=255)

                                                                               gaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTctct                                                                        <  1:2784089/150‑1 (MQ=255)

                                                                               gaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTctct                                                                        >  1:2455883/1‑150 (MQ=255)

                                                                               gaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGCTGTTCTTTGCGTGCGTGTTTTACAGGCAAGCACATCCAGAGGAGATGCTCCCATCAGGCGAGCGTCAGTctct                                                                        >  2:4472991/1‑150 (MQ=255)

                                                                                 aGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTtc                                                                      <  1:4040739/150‑1 (MQ=255)

                                                                                  ggACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTtcc                                                                     <  1:4230895/150‑1 (MQ=255)

                                                                                   gACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTtcct                                                                    <  1:142496/150‑1 (MQ=255)

                                                                                      caccTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCa                                                                 >  1:1113714/1‑150 (MQ=255)

                                                                                          tGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCaa                                                             <  1:1440708/150‑1 (MQ=255)

                                                                                           gCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAAc                                                            >  1:3470852/1‑150 (MQ=255)

                                                                                           gCCAGGATGGCGGGGCGATACGGATATCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAAc                                                            <  1:3765135/150‑1 (MQ=255)

                                                                                              aGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTg                                                         <  1:2297528/150‑1 (MQ=255)

                                                                                                gATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTgcg                                                       <  1:3621512/150‑1 (MQ=255)

                                                                                                gATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTgcg                                                       <  1:1492106/150‑1 (MQ=255)

                                                                                                     cGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCgcagca                                                  <  1:4475404/150‑1 (MQ=255)

                                                                                                     cGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCgcagca                                                  <  1:4348026/150‑1 (MQ=255)

                                                                                                      ggggCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCttt                                                                                                                                         <  2:854251/62‑1 (MQ=255)

                                                                                                      ggggCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCttt                                                                                                                                         >  1:854251/1‑62 (MQ=255)

                                                                                                      ggggCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCCGCAACGTGCGccgcag                                                 >  2:2568149/1‑150 (MQ=255)

                                                                                                      ggggCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGcagcag                                                 >  1:1184457/1‑150 (MQ=255)

                                                                                                       gggCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCagcagc                                                <  2:3457737/150‑1 (MQ=255)

                                                                                                             tACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCcaca                                          <  1:1552068/150‑1 (MQ=255)

                                                                                                              aCGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACag                                         >  1:258071/1‑150 (MQ=255)

                                                                                                              aCGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACag                                         >  1:257962/1‑150 (MQ=255)

                                                                                                                ggATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACagag                                       <  2:3898273/150‑1 (MQ=255)

                                                                                                                ggATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACagag                                       <  2:1058509/150‑1 (MQ=255)

                                                                                                                 gATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAgg                                      <  1:2388156/150‑1 (MQ=255)

                                                                                                                  aTGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGc                                     <  1:3851869/150‑1 (MQ=255)

                                                                                                                     tcgcaGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCctg                                  <  2:2194744/147‑1 (MQ=255)

                                                                                                                      cacaGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCctgc                                 >  2:730311/1‑150 (MQ=255)

                                                                                                                       acaGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCctgct                                >  2:2172247/1‑150 (MQ=255)

                                                                                                                         aGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTgg                              <  1:1827560/150‑1 (MQ=255)

                                                                                                                          gggAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgc                             >  2:1526801/1‑150 (MQ=255)

                                                                                                                           ggAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgcg                            <  1:732305/150‑1 (MQ=255)

                                                                                                                           ggAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgcg                            <  2:3137562/150‑1 (MQ=255)

                                                                                                                           ggAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgcg                            >  2:4454868/1‑150 (MQ=255)

                                                                                                                           ggAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgcg                            >  1:2408539/1‑150 (MQ=255)

                                                                                                                            gAAACAGTCTGGAACACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCCGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCACCGTGCGCAGCAGCTCCCCAGAGGCctgctgttcg                           <  2:4104288/150‑5 (MQ=255)

                                                                                                                            gAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTATCTTTGCGTGCGTGATATTCAGGCCAGCACATCCAGAGGTGATGCTCCCATCAGTCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgcgc                           >  1:2983277/1‑150 (MQ=255)

                                                                                                                               aCAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                        <  1:579046/150‑1 (MQ=255)

                                                                                                                               aCAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                        <  2:1321505/150‑1 (MQ=255)

                                                                                                                                 aGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGc                      >  1:2334765/1‑150 (MQ=255)

                                                                                                                                   tCTGGAAATCCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCAt                    >  2:283350/1‑150 (MQ=255)

                                                                                                                                   tCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCAt                    >  1:68735/1‑150 (MQ=255)

                                                                                                                                    cTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATc                   <  2:694389/150‑1 (MQ=255)

                                                                                                                                      agAAAACCCCGCCGAAGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATcgc                 >  1:3788138/2‑150 (MQ=255)

                                                                                                                                         aaaCCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGaa              <  2:2953653/150‑1 (MQ=255)

                                                                                                                                          aaCCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGaaa             <  2:1387360/150‑1 (MQ=255)

                                                                                                                                           aCCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAAt            <  2:3232465/150‑1 (MQ=255)

                                                                                                                                              ccGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGaaa             >  1:2652350/1‑146 (MQ=255)

                                                                                                                                               cGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGt                                                          >  2:4221811/1‑100 (MQ=255)

                                                                                                                                               cGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCa        >  2:1772484/1‑150 (MQ=255)

                                                                                                                                                 cTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCAgg      >  1:2841329/1‑150 (MQ=255)

                                                                                                                                                     ggcggGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGAGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCAGGCCGa  >  2:3769273/1‑150 (MQ=255)

TCTAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCAGGCCGA  >  NC_002947/450775‑451073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`