Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,094,120 A→C 8.7% E189D (GAA→GAC)  PP_5343 → putative Transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,094,1200AC 8.7% 648.5 / 3.2 214E189D (GAA→GAC) PP_5343putative Transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (164/17);  new base C (2/24);  total (167/47)
Fisher's exact test for biased strand distribution p-value = 2.65e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

GAACCATGGAAAGTCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCTTTGGCAGGCCTACC  >  NC_002947/6093971‑6094267
                                                                                                                                                     |                                                                                                                                                   
gAACCATGGAAAGTCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGaa                                                                                                                                                     <  1:3422725/150‑1 (MQ=255)
   ccctGGAAAGTCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCACGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGc                                                                                                                                                  <  2:4050712/147‑1 (MQ=255)
     aTGGAAAGTCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCt                                                                                                                                                >  2:2197311/1‑150 (MQ=255)
      tGGAAAGTCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTg                                                                                                                                               >  1:4302002/1‑150 (MQ=255)
      aaaaaaGTAAACGACGCCACTGAAGACTAAATCCAGGGCATGCTCCGCGCCATCGACGGTATCCGTCTGCCCATCGCTCGCCTCCAAGGTGCTCGCAACCTCAGCCAGAACCGCTCCGGGCAAGACATAGCTGGTGTGCGCGAAGCCCTg                                                                                                                                               >  2:10902/4‑150 (MQ=255)
       ggAAAGTCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGa                                                                                                                                              >  2:1250098/1‑150 (MQ=255)
       ggAAAGTCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGa                                                                                                                                              <  2:2866040/150‑1 (MQ=255)
       ggAAAGTCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGa                                                                                                                                              <  2:2082496/150‑1 (MQ=255)
         aaaGTCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAgc                                                                                                                                            <  1:1667597/150‑1 (MQ=255)
            gTCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGcc                                                                                                                                         <  1:2587784/150‑1 (MQ=255)
            gTCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGcc                                                                                                                                         <  1:2219208/150‑1 (MQ=255)
             tCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCTCAGGCCTTCgcgcac                                                                                                                                        <  1:3239459/150‑3 (MQ=255)
             tCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCa                                                                                                                                        >  2:1993558/1‑150 (MQ=255)
             tCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCa                                                                                                                                        >  1:803561/1‑150 (MQ=255)
             tCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCa                                                                                                                                        >  1:2481662/1‑150 (MQ=255)
                 cgccGCCCCGGCCGACTACTTGGTGGGCTTGCTCCGCGCCTTGGTGGGTATGCGTCTGCCCTGGGCGCGCCTGCTTGGTGCTCGCAAGCACAGCCAGAACCGCTCGGGTAAAGACATGTGTGGTGTGCGGGAAGGCCTGAGCGCCAGccc                                                                                                                                    <  2:351540/147‑1 (MQ=255)
                 cGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGccc                                                                                                                                    >  1:1797551/1‑150 (MQ=255)
                 cGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGccc                                                                                                                                    >  2:1070341/1‑150 (MQ=255)
                      ccccTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATc                                                                                                                               >  1:4351511/1‑150 (MQ=255)
                       cccTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGATGGCCTGAGCGCCAGCCCCGATCa                                                                                                                              >  1:1946171/1‑150 (MQ=255)
                       cccTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCa                                                                                                                              >  2:1287870/1‑150 (MQ=255)
                           gCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTg                                                                                                                          >  2:2344360/1‑150 (MQ=255)
                            ccGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTgg                                                                                                                         >  2:4318303/1‑150 (MQ=255)
                              gACTACATGGTGGGCTGGCGCCGCGCCATGGGCGGTATCGGTCGGCCCCGCGCGCGCCTGCACGGTGCGCCCAAGCTCAGCCCCAACGGCTCGGGGAAAGACATCGGTGGTGTGCGCGACGGCCTGCGCGCCAGCCCCGATCAACTGGAc                                                                                                                       <  2:3284822/150‑1 (MQ=255)
                              gACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGAc                                                                                                                       >  1:2333772/1‑150 (MQ=255)
                               aCTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACa                                                                                                                      >  2:597510/1‑150 (MQ=255)
                                 tACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAAt                                                                                                                    >  2:2145241/1‑150 (MQ=255)
                                 tACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAAt                                                                                                                    >  1:4102207/1‑150 (MQ=255)
                                 tACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAAt                                                                                                                    >  1:1238328/1‑150 (MQ=255)
                                  ccATCGTGGGCTTGCGCGGCGCCTTGGTGTGTATCCGTCTGCCCCGGGCGCGCCTGCTGGGTGCGCGAAAGCTCAGCCAGAACGGCTCGGGTAAAGACTTGGGTGGTGTGCGCTACGGCGTGCGCGCCAGCCCCGATCAACTGGACAATc                                                                                                                   <  2:4472395/149‑1 (MQ=255)
                                  aCATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATc                                                                                                                   >  1:3442945/1‑150 (MQ=255)
                                   gccaaaGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCa                                                                                                                  >  2:3320807/6‑150 (MQ=255)
                                   cATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACACTCa                                                                                                                  >  2:3676065/1‑150 (MQ=255)
                                   cATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCa                                                                                                                  >  2:662303/1‑150 (MQ=255)
                                    aTCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCaa                                                                                                                 >  1:77505/1‑150 (MQ=255)
                                     tCGTGGGCATGCTCCGCGCCTTGGGGGGTATCCGTCTGCCCCGCGCGCGCGTGCCGGGTGCGCGCCCGCACCGCCCGACCGGCTGCGGGAATGACCTGGGTGGTGTGCGCGCTGGCCTGCGCGCCAGCCCCGATCAACTGGACAATCAAc                                                                                                                <  2:4429367/150‑1 (MQ=255)
                                     tCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAAc                                                                                                                >  2:4499556/1‑150 (MQ=255)
                                          ggCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTcgcg                                                                                                           >  2:3357975/1‑150 (MQ=255)
                                             aTGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCgcggcg                                                                                                        >  1:606108/1‑150 (MQ=255)
                                                 tCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGcaca                                                                                                    >  2:2859907/1‑150 (MQ=255)
                                                          tcgtcgGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAAc                                                                                           >  2:2678099/1‑150 (MQ=255)
                                                          tcgtcgGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAAc                                                                                           >  2:3401447/1‑150 (MQ=255)
                                                             tcgGTATCCGTCTGCCCCCCGCGCGCCGGCACGGGGCGCCCACCCCCAGCCCGACCCGCTCGGGGCAAGACTGGGCTGGTGTGCGCGACGGCGTGAGCCCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAActct                                                                                        <  2:1424504/150‑1 (MQ=255)
                                                              cgGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTg                                                                                       >  1:3736134/1‑150 (MQ=255)
                                                                ttatggttCTGCCCATGGGGCGGTTTCTTGGTGCGCGCAAGCTCAGCAAGACCCGCTCGGGGAATGACTTCGGTGGTGGGCGCGACGGCCTGAGCCCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGaa                                                                                     <  2:3003192/143‑1 (MQ=255)
                                                                gtctCCGTCTGCCCAGCGCGCGCCTGCCCGGGGCGCGCAAGCTCAGCCAGACCCGCTCCGGGCAAGACATCGGGGGTGTGCGCGAAGGCCCGCGCCCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGaa                                                                                     <  1:671689/147‑1 (MQ=255)
                                                                gTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGaa                                                                                     >  1:1534697/1‑150 (MQ=255)
                                                                  aTCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAgg                                                                                   >  1:4087229/1‑150 (MQ=255)
                                                                     cGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAAt                                                                                >  1:2320390/1‑150 (MQ=255)
                                                                       tCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAg                                                                              >  1:840225/1‑150 (MQ=255)
                                                                           cccATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCa                                                                          >  1:2156446/1‑150 (MQ=255)
                                                                             cctggcgcgcCTGCTGGGGGCGCGCACCCTCCCCCACAACCGCGCGGGGCAATACTTGGGGGGTGTGCGGGAGGGCGTGCGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATg                                                                        <  2:1197315/146‑1 (MQ=255)
                                                                              aTCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGc                                                                       >  2:167249/1‑150 (MQ=255)
                                                                                   gcgcCTGCATGGTGCGCGCAAGCTCAGCCAGAACCGCTCGGGGCAAGACTGCGCGGGTGTGCGCGACGGCCTGCGCCCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAAc                                                                  <  2:1557293/150‑1 (MQ=255)
                                                                                   gcgcCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAAc                                                                  >  1:240577/1‑150 (MQ=255)
                                                                                   gcgcCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAAc                                                                  >  1:4491985/1‑150 (MQ=255)
                                                                                     gcCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCt                                                                >  1:1563259/1‑150 (MQ=255)
                                                                                       cTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTa                                                              >  2:2931970/1‑150 (MQ=255)
                                                                                        tGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTAc                                                             >  2:1036591/1‑150 (MQ=255)
                                                                                        tGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTAc                                                             >  2:845192/1‑150 (MQ=255)
                                                                                        tGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTAc                                                             >  2:3362136/1‑150 (MQ=255)
                                                                                        tGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTAc                                                             >  2:3362309/1‑150 (MQ=255)
                                                                                         gCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTAcc                                                            >  1:4369493/1‑150 (MQ=255)
                                                                                          cAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTAccc                                                           >  1:2125867/1‑150 (MQ=255)
                                                                                           aaGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCt                                                          >  1:1269486/1‑150 (MQ=255)
                                                                                             ggTGCGCCCCAGCTCCGCCAGACCCGCTCGGGGCTTGACCGCGCGGGTGTGCCCGCCGCCCCCCCCCCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTgc                                                        <  1:3309096/150‑1 (MQ=255)
                                                                                              gTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTgcg                                                       >  2:1150229/1‑150 (MQ=255)
                                                                                                gcgcgcAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCCTGCCCAACCTTACCCTGCGcc                                                     >  1:328530/1‑150 (MQ=255)
                                                                                                    gcACGCCCACCCACACCCGCGCCGGGCAATACTTGGTGGTTGTGCGCGATGGCCTGCGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGt                                                 <  2:1740806/150‑1 (MQ=255)
                                                                                                    gcAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGt                                                 >  2:1277086/1‑150 (MQ=255)
                                                                                                          tCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGc                                           >  2:4002678/1‑150 (MQ=255)
                                                                                                          tCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGc                                           >  1:3491331/1‑150 (MQ=255)
                                                                                                             gCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGACGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCa                                        >  2:101401/1‑150 (MQ=255)
                                                                                                              ccAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCaa                                       >  1:1720971/1‑150 (MQ=255)
                                                                                                               cAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAg                                      >  1:1801650/1‑150 (MQ=255)
                                                                                                               cAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAg                                      >  2:1504350/1‑150 (MQ=255)
                                                                                                                 gAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGAt                                    >  2:4150459/1‑150 (MQ=255)
                                                                                                                   aCCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCa                                  >  2:1428254/1‑150 (MQ=255)
                                                                                                                        tCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAg                             >  2:907001/1‑150 (MQ=255)
                                                                                                                        tCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAg                             >  1:262745/1‑150 (MQ=255)
                                                                                                                        tCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAg                             >  1:3738528/1‑150 (MQ=255)
                                                                                                                        cgggcccAAGACTTGGGTGGTGTGCGCCAAGGCCTGCGCCCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAg                             <  2:3906897/144‑1 (MQ=255)
                                                                                                                          cGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGcc                           >  1:731449/1‑150 (MQ=255)
                                                                                                                              cAAGACCTGGGTGGTGTGCGCGACGGCCTGAGCCCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGc                       <  2:563681/150‑1 (MQ=255)
                                                                                                                               aaGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGcc                      >  1:1349639/1‑149 (MQ=255)
                                                                                                                               aaGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCa                      >  1:1485030/1‑150 (MQ=255)
                                                                                                                                  aCATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGc                   >  2:278134/1‑150 (MQ=255)
                                                                                                                                    aTCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGccc                 >  2:2157706/1‑150 (MQ=255)
                                                                                                                                     tCGGTGGTGTGGGCCACGGCCTGCGCCCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCt                <  2:3825887/150‑1 (MQ=255)
                                                                                                                                     tCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCt                >  1:3845821/1‑150 (MQ=255)
                                                                                                                                     tCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCt                >  1:997323/1‑150 (MQ=255)
                                                                                                                                     tCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCt                >  2:2641092/1‑150 (MQ=255)
                                                                                                                                     tCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCt                >  2:475463/1‑150 (MQ=255)
                                                                                                                                       gCTGGGGCGCGCCACGGCGCGAGCCCCAGCCCCGATCAAGTGGACAATCAACTCGCGTATCACATGCGGAAACTCGGAAGGAATAGCCCATGCCCAACCTTACACTGCGCCCTGTCACGGCCCTAGATCACGCAGCCTGGCACGCCCttt              <  1:10902/150‑1 (MQ=255)
                                                                                                                                        ggggTGTGCGCGTTGGCCTGCGCCCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCTTTg             <  2:4102207/148‑1 (MQ=255)
                                                                                                                                            tgggcgcGACGCCCCGCGCCCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCTTTGGCAg         <  1:794048/147‑1 (MQ=255)
                                                                                                                                              ggcgcgcCGGCCTGCGCCCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCTTTGGCAGGc       <  2:4382229/149‑1 (MQ=255)
                                                                                                                                                cgcgAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGAGCACAAGCGCCAACTCTGAAGGAATAGCCCATGCCCAACATAACCCTGAGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCGATGGCAGGCCt     >  1:3093509/1‑150 (MQ=255)
                                                                                                                                                 gcgAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCTTTGGCAGGCCTa    >  2:445287/1‑150 (MQ=255)
                                                                                                                                                   gAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCTTTGGCAGGCCTAcc  >  2:1908433/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GAACCATGGAAAGTCACCGACGCCCCTGCCGACTACATCGAGGGCATGCTCCGCGCCATCGTCGGTATCCGTCTGCCCATCGCGCGCCTGCAAGGTGCGCGCAAGCTCAGCCAGAACCGCTCCGGGCAAGACATCGCTGGTGTGCGCGAAGGCCTGAGCGCCAGCCCCGATCAACTGGACAATCAACTCGCGGCGCACATGCGCCAACTCTGAAGGAATAGCCCATGCCCAACCTTACCCTGCGCCCTGTCACGGCCCAAGATCACGCAGCCTGGCACGCCCTTTGGCAGGCCTACC  >  NC_002947/6093971‑6094267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑