BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,113,955	T→A	7.1%	H118Q (CAT→CAA)	PP_5362 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,113,955	0	T	A	7.1%	644.1 / 28.8	198	H118Q (CAT→CAA)	PP_5362	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (90/93); new base A (7/7); total (98/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

Only 100 of 204 total aligned reads displayed.

TGGGCACAAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGCCGTAACCG  >  NC_002947/6113806‑6114104

tgGGCACAAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCAt                                                                                                                                                       >  2:3608162/1‑150 (MQ=255)

 gggCACAAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCAtt                                                                                                                                                      >  2:1930453/1‑150 (MQ=255)

    cacaAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCa                                                                                                                                                   <  1:2969900/150‑1 (MQ=255)

    cacaAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCa                                                                                                                                                   <  2:1313138/150‑1 (MQ=255)

    cacaAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCa                                                                                                                                                   <  2:1180116/150‑1 (MQ=255)

    cacaAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCa                                                                                                                                                   <  2:73345/150‑1 (MQ=255)

     acaAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAg                                                                                                                                                  >  2:2478681/1‑150 (MQ=255)

       aaaCCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCt                                                                                                                                                <  1:1778002/150‑1 (MQ=255)

       aaaCCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCt                                                                                                                                                >  1:258134/1‑150 (MQ=255)

       aaaCCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCt                                                                                                                                                >  2:120743/1‑150 (MQ=255)

       aaaCCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCt                                                                                                                                                >  1:2619735/1‑150 (MQ=255)

           cAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGAt                                                                                                                                            >  2:4344046/1‑150 (MQ=255)

           cAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCAATGCAGCTTGaa                                                                                                                                            <  2:4250909/150‑2 (MQ=255)

           cAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCAATGCAGCTTGaa                                                                                                                                            <  2:4325115/150‑2 (MQ=255)

           cAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCAATGCAGCTTGaa                                                                                                                                            <  2:2793767/150‑2 (MQ=255)

           cAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCAATGCAGCTTGaa                                                                                                                                            <  2:1429416/150‑2 (MQ=255)

            aaCAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATc                                                                                                                                           >  1:4159887/1‑150 (MQ=255)

               aGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAg                                                                                                                                        >  1:3695110/1‑150 (MQ=255)

                gCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAgg                                                                                                                                       <  1:212672/150‑1 (MQ=255)

                 ccGCTATGGCACATTGAC‑TTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAgggg                                                                                                                                     >  2:2616361/1‑150 (MQ=255)

                  cGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAgggg                                                                                                                                     <  2:3952755/150‑1 (MQ=255)

                         gCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGtt                                                                                                                              >  1:2507954/1‑150 (MQ=255)

                           acaTTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGa                                                                                                                            >  1:3137404/1‑150 (MQ=255)

                           acaTTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGa                                                                                                                            <  2:1065063/150‑1 (MQ=255)

                           acaTTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGa                                                                                                                            >  2:3181368/1‑150 (MQ=255)

                              ttGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGc                                                                                                                         <  2:1824293/150‑1 (MQ=255)

                               tGACTTTTTGTCCCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGcc                                                                                                                        <  2:773864/150‑1 (MQ=255)

                                 aCTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGcctc                                                                                                                      >  1:4395397/1‑150 (MQ=255)

                                 aCTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGcctc                                                                                                                      >  2:780502/1‑150 (MQ=255)

                                   tttttttACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGcctcct                                                                                                                    >  1:1820525/1‑150 (MQ=255)

                                    ttttttACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCtt                                                                                                                   <  1:3971770/150‑1 (MQ=255)

                                         tACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCAtt                                                                                                              >  2:3705288/1‑150 (MQ=255)

                                           ccGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCAtttt                                                                                                            >  2:2654535/1‑150 (MQ=255)

                                            cGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTg                                                                                                           >  1:1070147/1‑150 (MQ=255)

                                            cGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTg                                                                                                           >  2:1405136/1‑150 (MQ=255)

                                              gCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGaa                                                                                                         <  2:3291632/150‑1 (MQ=255)

                                                cTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTGTGAACg                                                                                                       >  2:337691/1‑150 (MQ=255)

                                                   gCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGttt                                                                                                    >  1:856955/1‑150 (MQ=255)

                                                    cctgCGATGGTGAGGCCTGGGTTGGATACAGCCGCCGCGTTGGCTGTACCACCTTGAAACGCGTATCAAGTTCCTGCGGGGAGCGATTCAAGCTGCTTTGCTGATTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGtttt                                                                                                   <  1:3055345/147‑1 (MQ=255)

                                                     cAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGttttt                                                                                                  >  1:272716/1‑150 (MQ=255)

                                                     cAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGttttt                                                                                                  <  1:2078183/150‑1 (MQ=255)

                                                      aGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGtttttt                                                                                                 <  1:4214418/150‑1 (MQ=255)

                                                       gCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTtc                                                                                                >  2:3074382/1‑150 (MQ=255)

                                                        cGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTtct                                                                                               >  1:979707/1‑150 (MQ=255)

                                                          aTGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTctct                                                                                             >  2:1504394/1‑150 (MQ=255)

                                                          aTGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTctct                                                                                             <  1:791395/150‑1 (MQ=255)

                                                                     tgggtgggCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTAttt                                                                                  >  1:2951373/1‑150 (MQ=255)

                                                                     tgggtgggCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTAttt                                                                                  <  1:3607869/150‑1 (MQ=255)

                                                                     tgggtgggCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTGGTTTCTCTGCTGCCTAttt                                                                                  >  2:2575606/1‑150 (MQ=255)

                                                                         tgggCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCTGTTCCAGCGGGGAGCGCTTCACGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACCCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTa                                                                              <  2:1389648/150‑1 (MQ=255)

                                                                                  gccgccGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCtt                                                                     >  1:1631746/1‑150 (MQ=255)

                                                                                   ccgccgCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTc                                                                    >  1:2364328/1‑150 (MQ=255)

                                                                                     gccgcGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGa                                                                  <  2:1070210/150‑1 (MQ=255)

                                                                                     gccgcGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGa                                                                  <  2:626901/150‑1 (MQ=255)

                                                                                     gccgcGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGa                                                                  <  2:2890594/150‑1 (MQ=255)

                                                                                      ccgcGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAg                                                                 <  2:348609/150‑1 (MQ=255)

                                                                                        gcgTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCa                                                               <  1:717831/150‑1 (MQ=255)

                                                                                                tGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGa                                                       <  1:1409167/150‑1 (MQ=255)

                                                                                                 ggACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGaa                                                      <  1:3344558/150‑1 (MQ=255)

                                                                                                  gACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGaaa                                                     >  1:3732690/1‑150 (MQ=255)

                                                                                                  gACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGaaa                                                     >  1:3763687/1‑150 (MQ=255)

                                                                                                   accaccTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGaaaa                                                    <  1:3915898/150‑1 (MQ=255)

                                                                                                   accaccTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGaaaa                                                    <  1:1835881/150‑1 (MQ=255)

                                                                                                    ccaccTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAac                                                   >  2:1870595/1‑150 (MQ=255)

                                                                                                     caccTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAaca                                                  >  2:2089118/1‑150 (MQ=255)

                                                                                                      accTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAacac                                                 <  1:4367393/150‑1 (MQ=255)

                                                                                                        cTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCa                                               >  1:2672783/1‑150 (MQ=255)

                                                                                                        cTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCa                                               >  1:2274958/1‑150 (MQ=255)

                                                                                                        cTTGAACCGCGTATCCAGTTCCACCGGGGAGCGCTTCAAGCTGCAGTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTGTCATTTTGAACGTTGGTGCTCTGCTGCCGATTTCGTACGTCAGCGTCGAGCACGCGCTGAACACAccc                                               >  2:4093011/1‑149 (MQ=255)

                                                                                                             aCCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCtt                                          <  2:157087/150‑1 (MQ=255)

                                                                                                             aCCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCtt                                          <  1:4097261/150‑1 (MQ=255)

                                                                                                             aCCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCtt                                          <  2:3363391/150‑1 (MQ=255)

                                                                                                              ccGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTg                                         >  2:2763157/1‑150 (MQ=255)

                                                                                                                gcgTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGAc                                       >  1:3039994/1‑150 (MQ=255)

                                                                                                                  gTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCa                                     >  2:1035169/1‑150 (MQ=255)

                                                                                                                      ccAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAAccc                                 >  1:2182874/1‑150 (MQ=255)

                                                                                                                         gTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCttt                              <  2:460673/150‑1 (MQ=255)

                                                                                                                         gTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCttt                              <  1:1963890/150‑1 (MQ=255)

                                                                                                                           tCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGt                            <  2:4159887/150‑1 (MQ=255)

                                                                                                                           tCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGt                            <  2:3311893/150‑1 (MQ=255)

                                                                                                                           tCCAGCGGGGAGCCCATCAAGCTGCATTCCAGCTTGATCGAGGGGAGCCGTTGACGCCACCTTTCAATTAGAACGATTTTTCTCAGCTCCCTATTTTGTAAGTCAGCTTCGACCACGCGCTGAAAACACCACCCATGACCAACCCTTTGt                            <  2:149442/150‑1 (MQ=255)

                                                                                                                             cAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTATTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCa                          >  1:1135995/1‑150 (MQ=255)

                                                                                                                              aGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAg                         >  1:2589927/1‑150 (MQ=255)

                                                                                                                               gCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGa                        <  2:4035850/150‑1 (MQ=255)

                                                                                                                                   ggAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGa                    >  1:3218315/1‑150 (MQ=255)

                                                                                                                                     cctcgatCAAGCTGCAATGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGaaa                  >  2:3413890/7‑150 (MQ=255)

                                                                                                                                     cctcgatCAAGCTGCAATGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGaaa                  >  2:4178505/7‑150 (MQ=255)

                                                                                                                                        gcTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGc               >  1:1478593/1‑150 (MQ=255)

                                                                                                                                         gatCAAGCTGCAATGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCt              >  2:1125294/3‑150 (MQ=255)

                                                                                                                                         gatCAAGCTGCAATGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCt              >  1:3252157/3‑150 (MQ=255)

                                                                                                                                         cTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCt              <  1:1405652/150‑1 (MQ=255)

                                                                                                                                          ttCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTa             >  1:446331/1‑150 (MQ=255)

                                                                                                                                          ttCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTa             >  2:3884345/1‑150 (MQ=255)

                                                                                                                                          ttCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTa             >  1:1579915/1‑150 (MQ=255)

                                                                                                                                             aaGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAAAGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGc          <  1:2537148/150‑1 (MQ=255)

                                                                                                                                             aaGCTGCAATGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGGCTATTTTGTAAGTCAGATTCGAGCACGCTCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGc          >  2:1329898/1‑150 (MQ=255)

                                                                                                                                                   cATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGCCGTAAc    <  1:1293123/150‑1 (MQ=255)

                                                                                                                                                    aTTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGCCGTAAcc   <  1:1819316/150‑1 (MQ=255)

                                                                                                                                                     ttGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGCCGTAACcg  >  2:2529897/1‑150 (MQ=255)

                                                                                                                                                     ttGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGCCGTAACcg  >  2:2041310/1‑150 (MQ=255)

TGGGCACAAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGCCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGCCGTAACCG  >  NC_002947/6113806‑6114104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`