Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 699,725 A→G 53.8% intergenic (+115/‑129) PP_t06 → / → PP_23SD tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947699,7250AG53.8% 16.4 / 316.2 225intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (50/54);  new base G (69/52);  total (119/106)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

Only 100 of 227 total aligned reads displayed.

GGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCG  >  NC_002947/699576‑699808
                                                                                                                                                      |                                                                                   
ggaggTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGa                                                                                     <  1:4425084/150‑1 (MQ=37)
    gTCAGCGGTTCGATCCCGCTTGGCTCCGCCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGa                                                                                 >  1:306143/1‑150 (MQ=255)
     tCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGaa                                                                                >  2:2275030/1‑150 (MQ=37)
       aGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAAGGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAaca                                                                              >  1:3492877/1‑150 (MQ=255)
          ggTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCa                                                                           <  1:773444/150‑1 (MQ=37)
          ggTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCa                                                                           <  2:753891/150‑1 (MQ=37)
            ttCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGt                                                                         >  2:3029995/1‑150 (MQ=37)
            ttCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGt                                                                         <  2:3113563/150‑1 (MQ=37)
             tCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGtt                                                                        <  1:1318547/150‑1 (MQ=37)
              cGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGttt                                                                       <  1:142895/150‑1 (MQ=37)
               gATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTc                                                                      <  2:394869/150‑1 (MQ=37)
               gATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTc                                                                      >  1:4143599/1‑150 (MQ=37)
                aTCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCa                                                                     <  1:1685839/150‑1 (MQ=37)
                aTCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCa                                                                     >  1:1003605/1‑150 (MQ=37)
                aTCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCa                                                                     >  2:1059930/1‑150 (MQ=37)
                aTCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCa                                                                     >  1:1523822/1‑150 (MQ=37)
                aTCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGACAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCa                                                                     <  2:1513105/150‑1 (MQ=255)
                 tCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCAc                                                                    >  2:141521/1‑150 (MQ=37)
                 tCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCAc                                                                    <  1:2010330/150‑1 (MQ=37)
                 tCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCAc                                                                    <  2:2734101/150‑1 (MQ=37)
                  cccGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCAct                                                                   <  1:1187206/150‑1 (MQ=37)
                  cccGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCAct                                                                   >  1:1363398/1‑150 (MQ=37)
                      cTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCActgctg                                                               <  1:1879230/150‑1 (MQ=37)
                      cTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCActgctg                                                               <  2:1342326/150‑1 (MQ=37)
                      cTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCActgctg                                                               <  1:3566923/150‑1 (MQ=37)
                       ttGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCtgg                                                              >  1:281464/1‑150 (MQ=37)
                       ttGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAAAGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTATGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCtgg                                                              >  1:655339/1‑150 (MQ=255)
                        tGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCtggt                                                             <  2:1781948/150‑1 (MQ=37)
                           cTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGa                                                          >  1:4092902/1‑150 (MQ=37)
                              caccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCa                                                       >  2:928732/1‑150 (MQ=37)
                              caccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCa                                                       <  2:3419047/150‑1 (MQ=37)
                               accacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAg                                                      >  1:2252323/1‑150 (MQ=37)
                                ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAgg                                                     <  1:2100762/150‑1 (MQ=37)
                                ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAgg                                                     <  2:4229604/150‑1 (MQ=37)
                                    tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGGTTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTaa                                                 <  2:4069816/150‑1 (MQ=255)
                                    tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTaa                                                 <  1:4279088/150‑1 (MQ=37)
                                    tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTaa                                                 >  1:3264752/1‑150 (MQ=37)
                                    tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTaa                                                 >  1:851225/1‑150 (MQ=37)
                                    tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTaa                                                 >  1:4336440/1‑150 (MQ=37)
                                     ttCGCCGTACGCAGTTACTTGACAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAg                                                <  2:4092129/150‑1 (MQ=37)
                                      tCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAgg                                               >  2:2864812/1‑150 (MQ=37)
                                       cGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGt                                              <  1:3899240/150‑1 (MQ=37)
                                            tACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAAt                                         >  2:1992086/1‑150 (MQ=37)
                                            tACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAAt                                         >  2:3186153/1‑150 (MQ=37)
                                               gCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtg                                      >  1:694335/1‑150 (MQ=37)
                                               gCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtg                                      >  1:835916/1‑150 (MQ=37)
                                               gCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtg                                      <  1:240476/150‑1 (MQ=37)
                                               gCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtg                                      <  1:3384771/150‑1 (MQ=37)
                                                cAGTTACTTGTCGGAACTTAGAAATGAACAGTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                     <  1:533458/150‑1 (MQ=255)
                                                cAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                     <  2:3593753/150‑1 (MQ=37)
                                                cAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                     <  2:448489/150‑1 (MQ=37)
                                                cAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                     <  2:1238269/150‑1 (MQ=37)
                                                cAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                     <  2:1368069/150‑1 (MQ=37)
                                                cAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                     <  2:5381/150‑1 (MQ=37)
                                                cAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                     <  1:38396/150‑1 (MQ=37)
                                                 aGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGTTCAGCAGTTTCTCTGCTGGTGGATCAGGCTAAGGTAAAATTtgtg                                    >  2:4050432/1‑150 (MQ=11)
                                                  gTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAGAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGa                                   <  1:1958336/150‑1 (MQ=255)
                                                  gTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGa                                   <  2:4035290/150‑1 (MQ=37)
                                                  gTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGa                                   <  1:3762542/150‑1 (MQ=37)
                                                  gTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGa                                   >  1:2691694/1‑150 (MQ=37)
                                                  gTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGa                                   <  2:2656442/150‑1 (MQ=37)
                                                  gTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGa                                   >  2:2177489/1‑150 (MQ=37)
                                                     aCTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTGTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGtt                                >  2:2548621/1‑150 (MQ=21)
                                                      cTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTc                               >  1:225030/1‑150 (MQ=21)
                                                      cTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTc                               <  2:468282/150‑1 (MQ=34)
                                                       ttGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCAGGCTTTTGTCAGATCGATCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCt                              >  1:2557059/1‑150 (MQ=255)
                                                         gTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGc                            <  1:1406782/150‑1 (MQ=21)
                                                          tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCt                           >  1:4337830/1‑150 (MQ=21)
                                                          tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCt                           <  2:187019/150‑1 (MQ=21)
                                                          tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCt                           >  2:4357938/1‑150 (MQ=21)
                                                           cAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTc                          >  1:569009/1‑150 (MQ=21)
                                                            aGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCg                         >  2:2302438/1‑150 (MQ=21)
                                                            aGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAgg                                                     <  1:2579859/122‑1 (MQ=35)
                                                            aGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAgg                                                     >  2:2579859/1‑122 (MQ=35)
                                                             gAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGa                        >  2:1638248/1‑150 (MQ=21)
                                                             gAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGa                        >  1:1550795/1‑150 (MQ=34)
                                                               aCTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCTGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaaa                      <  2:862054/150‑1 (MQ=21)
                                                               aCTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTTAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTTCTCGaaa                      <  1:1159533/150‑1 (MQ=255)
                                                               aCTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaaa                      <  2:2740096/150‑1 (MQ=34)
                                                                cTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAag                     >  1:1913833/1‑150 (MQ=21)
                                                                 ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAaga                    <  2:1833590/150‑1 (MQ=21)
                                                                 ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAaga                    <  1:2205024/150‑1 (MQ=21)
                                                                 ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAaga                    <  2:1696103/150‑1 (MQ=34)
                                                                 ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAaga                    >  1:1753466/1‑150 (MQ=34)
                                                                  tAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAagag                   >  1:1808405/1‑150 (MQ=21)
                                                                  caGAAATCAACATTCGAGCT‑GAATGTTCATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAagag                   >  2:79191/2‑150 (MQ=11)
                                                                      aaTGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAAc               <  1:1056945/150‑1 (MQ=34)
                                                                       aTGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACg              >  2:3120653/1‑150 (MQ=21)
                                                                        tcaaCATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGt             >  1:2646648/3‑150 (MQ=21)
                                                                        tGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGt             >  2:3778713/1‑150 (MQ=34)
                                                                         gAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTa            >  2:2172536/1‑150 (MQ=21)
                                                                         gAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTa            <  2:4006148/150‑1 (MQ=34)
                                                                         gAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTa            <  2:4336440/150‑1 (MQ=34)
                                                                         gAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTa            >  1:4501169/1‑150 (MQ=34)
                                                                             aTTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGaa        <  2:3274388/150‑1 (MQ=21)
                                                                              ttC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAAt       <  1:2837356/150‑1 (MQ=21)
                                                                                  aGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAAtttt    >  2:2456811/1‑150 (MQ=17)
                                                                                  aGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAAtttt    >  2:3918992/1‑150 (MQ=17)
                                                                                  aGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAAtttt    <  1:2943266/150‑1 (MQ=17)
                                                                                  aGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGAAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAAtttt    >  1:3947598/1‑150 (MQ=17)
                                                                                                                                                      |                                                                                   
GGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCG  >  NC_002947/699576‑699808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑