Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 727,319 A→T 6.3% W6R (TGG→AGG) ‡ PP_0621 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947727,3190AT 6.3% 735.4 / 25.9 218W6R (TGG→AGG) ‡PP_0621hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (110/94);  new base T (11/3);  total (121/97)
Fisher's exact test for biased strand distribution p-value = 9.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

GTGCGACTGCTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGCGGCA  >  NC_002947/727170‑727466
                                                                                                                                                     |                                                                                                                                                   
gTGCGACTGCTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCa                                                                                                                                                     >  1:2228182/1‑150 (MQ=255)
   cGACTGCTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAgg                                                                                                                                                  <  1:952886/150‑1 (MQ=255)
     aCTGCTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGcc                                                                                                                                                >  2:4507420/1‑150 (MQ=255)
          tggctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCt                                                                                                                                           >  1:4209286/1‑150 (MQ=255)
            gctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCttt                                                                                                                                         <  2:3957738/150‑1 (MQ=255)
             ctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCTTGGGCCTCCCtttt                                                                                                                                        <  2:3558610/150‑2 (MQ=255)
             ctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttc                                                                                                                                        <  2:453050/150‑1 (MQ=255)
             ctgACTTTGTATTAGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCtttt                                                                                                                                        <  1:2016765/150‑2 (MQ=255)
              tgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttca                                                                                                                                       <  1:2361199/150‑1 (MQ=255)
                  tttGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcattca                                                                                                                                   >  1:531764/1‑150 (MQ=255)
                  tttGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcattca                                                                                                                                   >  2:2656524/1‑150 (MQ=255)
                        tttGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTg                                                                                                                             >  2:4012161/1‑150 (MQ=255)
                        tttGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTg                                                                                                                             >  1:197225/1‑150 (MQ=255)
                         ttGCGCCACAGCCAGAACGCGGCGGCGAT‑AGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGaa                                                                                                                           >  1:3825219/1‑150 (MQ=255)
                          tGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGaa                                                                                                                           <  1:825411/150‑1 (MQ=255)
                          tGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGaa                                                                                                                           <  1:4155814/150‑1 (MQ=255)
                          tGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGaa                                                                                                                           >  2:2084455/1‑150 (MQ=255)
                          tGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGaa                                                                                                                           <  2:912302/150‑1 (MQ=255)
                            cgcCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAgg                                                                                                                         >  1:2327301/1‑150 (MQ=255)
                               cacaGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCa                                                                                                                      <  1:301067/150‑1 (MQ=255)
                               cacaGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCa                                                                                                                      <  1:1419153/150‑1 (MQ=255)
                               cacaGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCa                                                                                                                      >  1:1333500/1‑150 (MQ=255)
                                 caGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGt                                                                                                                    <  2:438922/150‑1 (MQ=255)
                                  aGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTc                                                                                                                   <  2:2777565/150‑1 (MQ=255)
                                      aGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAAc                                                                                                               >  1:2404958/1‑150 (MQ=255)
                                      aGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAAc                                                                                                               <  2:1333500/150‑1 (MQ=255)
                                          cgcgGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAATAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGAc                                                                                                           <  2:2279318/150‑1 (MQ=255)
                                          cgcgGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGAc                                                                                                           <  1:2931444/150‑1 (MQ=255)
                                               cggcgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAg                                                                                                      <  2:1569529/150‑1 (MQ=255)
                                                   gATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAATTGGGAGGCCCTAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGATCAGTCCAAAGGTCa                                                                                                  >  2:3746656/1‑150 (MQ=255)
                                                   gATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCa                                                                                                  >  2:3282075/1‑150 (MQ=255)
                                                            gATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTCTCGATTGAAGAAGCCAACACTCTACGACGCGGCCAATAAAAAAGCGAGGCCCAACGCCTCCCTTTCATTCACCGCTGAAGGACAGTCGAACAGACCAAAGGTCATGTAGCTGa                                                                                         <  2:1686781/150‑1 (MQ=255)
                                                                cAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCa                                                                                     <  2:676437/150‑1 (MQ=255)
                                                                 aGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAg                                                                                    >  1:1344714/1‑150 (MQ=255)
                                                                 aGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAg                                                                                    >  2:2879210/1‑150 (MQ=255)
                                                                   aaaaGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGa                                                                                  <  2:2287372/150‑1 (MQ=255)
                                                                    aaaGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGaa                                                                                 <  1:2086057/150‑1 (MQ=255)
                                                                       gTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACgg                                                                              >  1:4001179/1‑150 (MQ=255)
                                                                             gAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTtc                                                                        >  2:2480206/1‑150 (MQ=255)
                                                                             gAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTtc                                                                        >  2:843809/1‑150 (MQ=255)
                                                                             gAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTtc                                                                        <  1:459685/150‑1 (MQ=255)
                                                                              aaCCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTtct                                                                       >  1:1819952/1‑150 (MQ=255)
                                                                               aCCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTtctc                                                                      <  1:4089968/150‑1 (MQ=255)
                                                                                  aTGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAATAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGc                                                                   >  2:1849162/1‑150 (MQ=255)
                                                                                        tCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCttt                                                             <  1:2544513/150‑1 (MQ=255)
                                                                                         ccAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTc                                                            >  1:309592/1‑150 (MQ=255)
                                                                                         ccAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTc                                                            >  2:252166/1‑150 (MQ=255)
                                                                                          cAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCg                                                           >  1:262380/1‑150 (MQ=255)
                                                                                          cAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCg                                                           <  1:3848074/150‑1 (MQ=255)
                                                                                           aGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCgg                                                          <  1:4445121/150‑1 (MQ=255)
                                                                                           aGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCgg                                                          >  2:2067042/1‑150 (MQ=255)
                                                                                           aGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCgg                                                          <  2:218540/150‑1 (MQ=255)
                                                                                           aGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCgg                                                          >  2:4317814/1‑150 (MQ=255)
                                                                                             cTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGc                                                        <  2:3047570/150‑1 (MQ=255)
                                                                                              ttGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGcc                                                       >  1:1268248/1‑150 (MQ=255)
                                                                                              ttGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGcc                                                       >  1:4510145/1‑150 (MQ=255)
                                                                                                 tCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTc                                                    <  2:1507243/150‑1 (MQ=255)
                                                                                                  cGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTcc                                                   >  2:3126483/1‑150 (MQ=255)
                                                                                                      tGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCa                                               <  2:399309/150‑1 (MQ=255)
                                                                                                       gaagaaGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTTAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCAc                                              >  2:1472323/1‑150 (MQ=255)
                                                                                                       gaagaaGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCAc                                              <  2:4209286/150‑1 (MQ=255)
                                                                                                        aagaagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACg                                             >  2:4234227/1‑150 (MQ=255)
                                                                                                         agaagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGa                                            >  2:835583/1‑150 (MQ=255)
                                                                                                          gaagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGAt                                           <  2:3735202/150‑1 (MQ=255)
                                                                                                           aagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGAtt                                          >  1:3182025/1‑150 (MQ=255)
                                                                                                             gCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGATCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTgg                                        <  1:1481539/150‑1 (MQ=255)
                                                                                                               cAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGt                                      >  1:393641/1‑150 (MQ=255)
                                                                                                               cAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTC‑TTCGGGCCTTCCGGCACGATTGGGTc                                     >  2:3903130/1‑150 (MQ=255)
                                                                                                                aaCAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGcttc                                                                <  2:2014808/123‑1 (MQ=255)
                                                                                                                aaCAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTc                                     >  1:717330/1‑150 (MQ=255)
                                                                                                                aaCAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTc                                     >  2:1415241/1‑150 (MQ=255)
                                                                                                                     tCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTATCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCCGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGt                                >  2:2600336/1‑150 (MQ=255)
                                                                                                                      cTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGtt                               <  2:2327301/150‑1 (MQ=255)
                                                                                                                       tAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTtc                              >  1:1612320/1‑150 (MQ=255)
                                                                                                                       tAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTtc                              >  2:1907815/1‑150 (MQ=255)
                                                                                                                        aGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGAGTGGGTCGCCGTtct                             >  2:2444435/1‑150 (MQ=255)
                                                                                                                         ggACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTtctc                            >  2:4443593/1‑150 (MQ=255)
                                                                                                                             gcgGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTggg                                       <  1:1052543/135‑1 (MQ=255)
                                                                                                                             gcgGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCt                        <  1:2056856/150‑1 (MQ=255)
                                                                                                                             gcgGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCt                        <  1:907226/150‑1 (MQ=255)
                                                                                                                               ggCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCttt                      >  1:3302100/1‑150 (MQ=255)
                                                                                                                                gCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTa                     <  2:2622817/150‑1 (MQ=255)
                                                                                                                                gCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTa                     >  2:4118682/1‑150 (MQ=255)
                                                                                                                                 ccAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAg                    >  2:1983419/1‑150 (MQ=255)
                                                                                                                                  cAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAgg                   >  2:1753631/1‑150 (MQ=255)
                                                                                                                                  cAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAgg                   >  1:4321205/1‑150 (MQ=255)
                                                                                                                                  cAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAgg                   <  2:2866286/150‑1 (MQ=255)
                                                                                                                                       aaaaaGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGgcagca              >  1:1175284/1‑150 (MQ=255)
                                                                                                                                        aaaaGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTAGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGcagcag             >  2:3350103/1‑150 (MQ=255)
                                                                                                                                        aaaaGGGAGGCCCAAGGCCTCCCTGTCCTTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACCATTGGGTCGCCGTTCTCGTCTTTCGGcagcag             >  2:3350140/1‑150 (MQ=255)
                                                                                                                                         aaaGGGAGGCCTTGGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCagcagc            >  2:643681/1‑150 (MQ=255)
                                                                                                                                         aaaGGGAGGCCTTGGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCagcagc            >  2:1192576/1‑150 (MQ=255)
                                                                                                                                          aaGGGAGGCCTTGGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGct           >  1:3831972/1‑150 (MQ=255)
                                                                                                                                           aGGGAGGCCTTGGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGctc          >  2:334344/1‑150 (MQ=255)
                                                                                                                                             ggAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCtctc        >  1:4214057/1‑150 (MQ=255)
                                                                                                                                             ggAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCtctc        >  2:3669259/1‑150 (MQ=255)
                                                                                                                                             ggAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCtctc        >  1:3262251/1‑150 (MQ=255)
                                                                                                                                               aGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTcgc      <  1:1849162/150‑1 (MQ=255)
                                                                                                                                                   ccAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGCGGCa  >  2:3486160/1‑150 (MQ=255)
                                                                                                                                                   ccAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGCGGCa  >  1:884359/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GTGCGACTGCTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGCGGCA  >  NC_002947/727170‑727466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑